Restriction map for
pFN21AB4181
(Linear) (Six-base) MAPSORT of: KIBB4181.seq Check: 3523 from: 1 to: 2706
KIBB4181.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AatII G_ACGT'C
Cuts at: 0 1382 2706
Size: 1382 1324
AccI GT'mk_AC
Cuts at: 0 1382 1541 2706
Size: 1382 159 1165
Fragments arranged by size:
1382 1165 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 737 1576 2706
Size: 530 207 839 1130
Fragments arranged by size:
1130 839 530 207
AflIII A'CryG_T
Cuts at: 0 2390 2582 2706
Size: 2390 192 124
Fragments arranged by size:
2390 192 124
AleI CACnn'nnGTG
Cuts at: 0 1780 1957 2377 2706
Size: 1780 177 420 329
Fragments arranged by size:
1780 420 329 177
AlwNI CAG_nnn'CTG
Cuts at: 0 27 723 1885 2569 2706
Size: 27 696 1162 684 137
Fragments arranged by size:
1162 696 684 137 27
ApoI r'AATT_y
Cuts at: 0 2491 2706
Size: 2491 215
AvaI C'yCGr_G
Cuts at: 0 766 2474 2706
Size: 766 1708 232
Fragments arranged by size:
1708 766 232
AvrII C'CTAG_G
Cuts at: 0 331 2706
Size: 331 2375
BamHI G'GATC_C
Cuts at: 0 668 998 2706
Size: 668 330 1708
Fragments arranged by size:
1708 668 330
BanI G'GyrC_C
Cuts at: 0 335 687 762 1303 1534 2706
Size: 335 352 75 541 231 1172
Fragments arranged by size:
1172 541 352 335 231 75
BanII G_rGCy'C
Cuts at: 0 239 798 1627 2706
Size: 239 559 829 1079
Fragments arranged by size:
1079 829 559 239
BbeI G_GCGC'C
Cuts at: 0 1307 2706
Size: 1307 1399
BbsI GAAGACnn'nnnn_
Cuts at: 0 1031 1564 1753 2390 2505 2706
Size: 1031 533 189 637 115 201
Fragments arranged by size:
1031 637 533 201 189 115
BbvCI CC'TCA_GC
Cuts at: 0 551 575 2706
Size: 551 24 2131
Fragments arranged by size:
2131 551 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 1368 2706
Size: 1368 1338
BclI T'GATC_A
Cuts at: 0 1784 2429 2706
Size: 1784 645 277
Fragments arranged by size:
1784 645 277
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1762 2291 2706
Size: 32 1730 529 415
Fragments arranged by size:
1730 529 415 32
BglII A'GATC_T
Cuts at: 0 1479 2646 2706
Size: 1479 1167 60
Fragments arranged by size:
1479 1167 60
Bme1580I G_kGCm'C
Cuts at: 0 291 767 2706
Size: 291 476 1939
Fragments arranged by size:
1939 476 291
BmgBI CAC'GTC
Cuts at: 0 2601 2706
Size: 2601 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 2029 2706
Size: 645 1384 677
Fragments arranged by size:
1384 677 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1036 1132 2542 2706
Size: 901 135 96 1410 164
Fragments arranged by size:
1410 901 164 135 96
Bpu10I CC'TnA_GC
Cuts at: 0 551 575 1796 2706
Size: 551 24 1221 910
Fragments arranged by size:
1221 910 551 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 2706
Size: 961 1745
BsaI GGTCTCn'nnnn_
Cuts at: 0 425 1312 2706
Size: 425 887 1394
Fragments arranged by size:
1394 887 425
BsaAI yAC'GTr
Cuts at: 0 1224 1566 2585 2706
Size: 1224 342 1019 121
Fragments arranged by size:
1224 1019 342 121
BsaHI Gr'CG_yC
Cuts at: 0 1304 1379 2706
Size: 1304 75 1327
Fragments arranged by size:
1327 1304 75
BsaWI w'CCGG_w
Cuts at: 0 427 2706
Size: 427 2279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 140 170 1192 1222 2706
Size: 140 30 1022 30 1484
Fragments arranged by size:
1484 1022 140 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1213 1516 2422 2706
Size: 1213 303 906 284
Fragments arranged by size:
1213 906 303 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1230 2095 2706
Size: 550 680 865 611
Fragments arranged by size:
865 680 611 550
BsiHKAI G_wGCw'C
Cuts at: 0 239 464 484 2706
Size: 239 225 20 2222
Fragments arranged by size:
2222 239 225 20
BsmI GAATG_Cn'
Cuts at: 0 1850 2706
Size: 1850 856
BsmBI CGTCTCn'nnnn_
Cuts at: 0 767 2706
Size: 767 1939
Bsp1286I G_dGCh'C
Cuts at: 0 239 291 464 484 767 798 1627 2706
Size: 239 52 173 20 283 31 829 1079
Fragments arranged by size:
1079 829 283 239 173 52 31 20
BspHI T'CATG_A
Cuts at: 0 1973 2157 2426 2706
Size: 1973 184 269 280
Fragments arranged by size:
1973 280 269 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 2684 2706
Size: 443 2241 22
Fragments arranged by size:
2241 443 22
BsrBI CCG'CTC
Cuts at: 0 2608 2706
Size: 2608 98
BsrDI GCAATG_nn'
Cuts at: 0 2120 2706
Size: 2120 586
BsrFI r'CCGG_y
Cuts at: 0 153 645 1457 1846 2706
Size: 153 492 812 389 860
Fragments arranged by size:
860 812 492 389 153
BsrGI T'GTAC_A
Cuts at: 0 488 1133 1466 2706
Size: 488 645 333 1240
Fragments arranged by size:
1240 645 488 333
BstBI TT'CG_AA
Cuts at: 0 2699 2706
Size: 2699 7
BstEII G'GTnAC_C
Cuts at: 0 813 1800 2706
Size: 813 987 906
Fragments arranged by size:
987 906 813
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1014 1863 1959 2706
Size: 1014 849 96 747
Fragments arranged by size:
1014 849 747 96
BstYI r'GATC_y
Cuts at: 0 668 998 1479 1616 1882 2646 2706
Size: 668 330 481 137 266 764 60
Fragments arranged by size:
764 668 481 330 266 137 60
Bsu36I CC'TnA_GG
Cuts at: 0 55 581 2706
Size: 55 526 2125
Fragments arranged by size:
2125 526 55
BtgI C'CryG_G
Cuts at: 0 272 672 2235 2486 2706
Size: 272 400 1563 251 220
Fragments arranged by size:
1563 400 272 251 220
BtsI GCAGTG_nn'
Cuts at: 0 17 322 939 1241 1397 2706
Size: 17 305 617 302 156 1309
Fragments arranged by size:
1309 617 305 302 156 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2475 2510 2706
Size: 2475 35 196
Fragments arranged by size:
2475 196 35
DraIII CAC_nnn'GTG
Cuts at: 0 75 2265 2706
Size: 75 2190 441
Fragments arranged by size:
2190 441 75
EaeI y'GGCC_r
Cuts at: 0 33 350 802 1394 1508 1899 2341 2603
Size: 33 317 452 592 114 391 442 262
Cuts at: 2603 2706
Size: 103
Fragments arranged by size:
592 452 442 391 317 262 114 103
33
EarI CTCTTCn'nnn_
Cuts at: 0 508 1276 2180 2706
Size: 508 768 904 526
Fragments arranged by size:
904 768 526 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 424 2706
Size: 424 2282
EcoICRI GAG'CTC
Cuts at: 0 237 2706
Size: 237 2469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 737 901 1036 1132 1576 2542 2706
Size: 530 207 164 135 96 444 966 164
Fragments arranged by size:
966 530 444 207 164 164 135 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 58 584 1674 2065 2342 2706
Size: 58 526 1090 391 277 364
Fragments arranged by size:
1090 526 391 364 277 58
EcoO109I rG'GnC_Cy
Cuts at: 0 344 1521 2334 2535 2706
Size: 344 1177 813 201 171
Fragments arranged by size:
1177 813 344 201 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1428 2706
Size: 1428 1278
HaeII r_GCGC'y
Cuts at: 0 1307 2706
Size: 1307 1399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 818 850 1311 1343 2421 2453 2706
Size: 818 32 461 32 1078 32 253
Fragments arranged by size:
1078 818 461 253 32 32 32
HincII GTy'rAC
Cuts at: 0 1383 1869 2580 2706
Size: 1383 486 711 126
Fragments arranged by size:
1383 711 486 126
HpaI GTT'AAC
Cuts at: 0 1869 2706
Size: 1869 837
KasI G'GCGC_C
Cuts at: 0 1303 2706
Size: 1303 1403
MscI TGG'CCA
Cuts at: 0 35 1510 2706
Size: 35 1475 1196
Fragments arranged by size:
1475 1196 35
MslI CAynn'nnrTG
Cuts at: 0 271 1780 1922 1957 2377 2706
Size: 271 1509 142 35 420 329
Fragments arranged by size:
1509 420 329 271 142 35
MspA1I CmG'CkG
Cuts at: 0 674 879 996 1167 1753 2217 2706
Size: 674 205 117 171 586 464 489
Fragments arranged by size:
674 586 489 464 205 171 117
NaeI GCC'GGC
Cuts at: 0 647 1459 1848 2706
Size: 647 812 389 858
Fragments arranged by size:
858 812 647 389
NarI GG'CG_CC
Cuts at: 0 1304 2706
Size: 1304 1402
NcoI C'CATG_G
Cuts at: 0 272 2706
Size: 272 2434
NgoMIV G'CCGG_C
Cuts at: 0 645 1457 1846 2706
Size: 645 812 389 860
Fragments arranged by size:
860 812 645 389
NspI r_CATG'y
Cuts at: 0 1260 1575 2394 2706
Size: 1260 315 819 312
Fragments arranged by size:
1260 819 315 312
PasI CC'CwG_GG
Cuts at: 0 123 2706
Size: 123 2583
PciI A'CATG_T
Cuts at: 0 2390 2706
Size: 2390 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 225 359 404 1885 2706
Size: 75 150 134 45 1481 821
Fragments arranged by size:
1481 821 150 134 75 45
PfoI T'CCnGG_A
Cuts at: 0 464 1013 1741 2706
Size: 464 549 728 965
Fragments arranged by size:
965 728 549 464
PmlI CAC'GTG
Cuts at: 0 1566 2706
Size: 1566 1140
PpuMI rG'GwC_Cy
Cuts at: 0 2334 2706
Size: 2334 372
PstI C_TGCA'G
Cuts at: 0 259 446 2706
Size: 259 187 2260
Fragments arranged by size:
2260 259 187
PvuII CAG'CTG
Cuts at: 0 879 1167 1753 2706
Size: 879 288 586 953
Fragments arranged by size:
953 879 586 288
SacI G_AGCT'C
Cuts at: 0 239 2706
Size: 239 2467
SacII CC_GC'GG
Cuts at: 0 675 2706
Size: 675 2031
SalI G'TCGA_C
Cuts at: 0 1381 2706
Size: 1381 1325
SapI GCTCTTCn'nnn_
Cuts at: 0 1276 2706
Size: 1276 1430
ScaI AGT'ACT
Cuts at: 0 166 2281 2706
Size: 166 2115 425
Fragments arranged by size:
2115 425 166
SfcI C'TryA_G
Cuts at: 0 255 442 2706
Size: 255 187 2264
Fragments arranged by size:
2264 255 187
SfoI GGC'GCC
Cuts at: 0 1305 2706
Size: 1305 1401
SmlI C'TyrA_G
Cuts at: 0 940 2706
Size: 940 1766
SphI G_CATG'C
Cuts at: 0 1260 2706
Size: 1260 1446
SspI AAT'ATT
Cuts at: 0 873 2706
Size: 873 1833
StuI AGG'CCT
Cuts at: 0 709 758 2706
Size: 709 49 1948
Fragments arranged by size:
1948 709 49
StyI C'CwwG_G
Cuts at: 0 272 331 1403 2402 2706
Size: 272 59 1072 999 304
Fragments arranged by size:
1072 999 304 272 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 2611 2706
Size: 70 2541 95
Fragments arranged by size:
2541 95 70
TatI w'GTAC_w
Cuts at: 0 164 488 1133 1466 2279 2706
Size: 164 324 645 333 813 427
Fragments arranged by size:
813 645 427 333 324 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1192 1224 1425 1457 2706
Size: 1192 32 201 32 1249
Fragments arranged by size:
1249 1192 201 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1273 1521 2706
Size: 1273 248 1185
Fragments arranged by size:
1273 1185 248
ZraI GAC'GTC
Cuts at: 0 1380 2706
Size: 1380 1326
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HpaI KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII
SacI SacII SalI SapI ScaI SfcI SfoI SmlI
SphI SspI StuI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BcgI
BfrBI BlpI BmtI BplI BsaBI BseYI BsiEI BsiWI
BspEI BssHII BssSI BstAPI BstZ17I BtgZI ClaI DraI
DrdI EagI EcoRI EcoRV FseI FspI FspAI HindIII
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI