Restriction map for
pFN21AB4048
(Linear) (Six-base) MAPSORT of: KIBB4048.seq Check: 5316 from: 1 to: 1788
KIBB4048.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
Acc65I G'GTAC_C
Cuts at: 0 1605 1788
Size: 1605 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1073 1184 1498 1788
Size: 736 337 111 314 290
Fragments arranged by size:
736 337 314 290 111
AflIII A'CryG_T
Cuts at: 0 167 1788
Size: 167 1621
ApaLI G'TGCA_C
Cuts at: 0 383 1788
Size: 383 1405
ApoI r'AATT_y
Cuts at: 0 1268 1433 1636 1732 1788
Size: 1268 165 203 96 56
Fragments arranged by size:
1268 203 165 96 56
BanI G'GyrC_C
Cuts at: 0 1605 1788
Size: 1605 183
BanII G_rGCy'C
Cuts at: 0 1314 1375 1788
Size: 1314 61 413
Fragments arranged by size:
1314 413 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 1788
Size: 742 1046
BclI T'GATC_A
Cuts at: 0 1722 1788
Size: 1722 66
BfrBI ATG'CAT
Cuts at: 0 624 1788
Size: 624 1164
Bme1580I G_kGCm'C
Cuts at: 0 387 1788
Size: 387 1401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1676 1788
Size: 414 1262 112
Fragments arranged by size:
1262 414 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 218 1007 1355 1788
Size: 218 789 348 433
Fragments arranged by size:
789 433 348 218
BsaHI Gr'CG_yC
Cuts at: 0 495 1788
Size: 495 1293
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1025 1292 1788
Size: 1025 267 496
Fragments arranged by size:
1025 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1788
Size: 453 1335
BsiEI CG_ry'CG
Cuts at: 0 41 1788
Size: 41 1747
BsiHKAI G_wGCw'C
Cuts at: 0 387 456 1325 1375 1788
Size: 387 69 869 50 413
Fragments arranged by size:
869 413 387 69 50
BsmI GAATG_Cn'
Cuts at: 0 576 624 954 1788
Size: 576 48 330 834
Fragments arranged by size:
834 576 330 48
Bsp1286I G_dGCh'C
Cuts at: 0 387 456 1314 1325 1375 1788
Size: 387 69 858 11 50 413
Fragments arranged by size:
858 413 387 69 50 11
BspHI T'CATG_A
Cuts at: 0 107 669 1107 1788
Size: 107 562 438 681
Fragments arranged by size:
681 562 438 107
BsrDI GCAATG_nn'
Cuts at: 0 159 713 848 1256 1788
Size: 159 554 135 408 532
Fragments arranged by size:
554 532 408 159 135
BsrGI T'GTAC_A
Cuts at: 0 232 1788
Size: 232 1556
BstAPI GCAn_nnn'nTGC
Cuts at: 0 518 582 1788
Size: 518 64 1206
Fragments arranged by size:
1206 518 64
Bsu36I CC'TnA_GG
Cuts at: 0 1502 1788
Size: 1502 286
BtgI C'CryG_G
Cuts at: 0 96 605 1788
Size: 96 509 1183
Fragments arranged by size:
1183 509 96
DraI TTT'AAA
Cuts at: 0 1617 1788
Size: 1617 171
EaeI y'GGCC_r
Cuts at: 0 417 1213 1788
Size: 417 796 575
Fragments arranged by size:
796 575 417
EarI CTCTTCn'nnn_
Cuts at: 0 199 777 1414 1474 1788
Size: 199 578 637 60 314
Fragments arranged by size:
637 578 314 199 60
EcoICRI GAG'CTC
Cuts at: 0 1373 1788
Size: 1373 415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 736 1073 1184 1498 1676 1788
Size: 414 322 337 111 314 178 112
Fragments arranged by size:
414 337 322 314 178 112 111
EcoNI CCTnn'n_nnAGG
Cuts at: 0 350 1529 1788
Size: 350 1179 259
Fragments arranged by size:
1179 350 259
EcoO109I rG'GnC_Cy
Cuts at: 0 144 1497 1788
Size: 144 1353 291
Fragments arranged by size:
1353 291 144
EcoRV GAT'ATC
Cuts at: 0 105 1788
Size: 105 1683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 333 365 601 633 666 698 910 942
Size: 333 32 236 32 33 32 212 32
Cuts at: 942 1788
Size: 846
Fragments arranged by size:
846 333 236 212 33 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1302 1788
Size: 1302 486
HindIII A'AGCT_T
Cuts at: 0 400 731 1788
Size: 400 331 1057
Fragments arranged by size:
1057 400 331
KpnI G_GTAC'C
Cuts at: 0 1609 1788
Size: 1609 179
MfeI C'AATT_G
Cuts at: 0 560 1788
Size: 560 1228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1788
Size: 230 1558
MscI TGG'CCA
Cuts at: 0 419 1788
Size: 419 1369
MspA1I CmG'CkG
Cuts at: 0 56 98 392 1788
Size: 56 42 294 1396
Fragments arranged by size:
1396 294 56 42
NcoI C'CATG_G
Cuts at: 0 605 1788
Size: 605 1183
NdeI CA'TA_TG
Cuts at: 0 1028 1788
Size: 1028 760
NsiI A_TGCA'T
Cuts at: 0 626 1788
Size: 626 1162
NspI r_CATG'y
Cuts at: 0 171 913 1063 1197 1788
Size: 171 742 150 134 591
Fragments arranged by size:
742 591 171 150 134
PciI A'CATG_T
Cuts at: 0 167 1788
Size: 167 1621
PflMI CCAn_nnn'nTGG
Cuts at: 0 206 1788
Size: 206 1582
PfoI T'CCnGG_A
Cuts at: 0 351 1788
Size: 351 1437
PpuMI rG'GwC_Cy
Cuts at: 0 1497 1788
Size: 1497 291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 794 826 1788
Size: 794 32 962
Fragments arranged by size:
962 794 32
PstI C_TGCA'G
Cuts at: 0 1080 1776 1788
Size: 1080 696 12
Fragments arranged by size:
1080 696 12
SacI G_AGCT'C
Cuts at: 0 1375 1788
Size: 1375 413
SacII CC_GC'GG
Cuts at: 0 99 1788
Size: 99 1689
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1788
Size: 199 1589
ScaI AGT'ACT
Cuts at: 0 803 1788
Size: 803 985
SexAI A'CCwGG_T
Cuts at: 0 71 1788
Size: 71 1717
SfcI C'TryA_G
Cuts at: 0 1076 1772 1788
Size: 1076 696 16
Fragments arranged by size:
1076 696 16
SspI AAT'ATT
Cuts at: 0 1626 1788
Size: 1626 162
StyI C'CwwG_G
Cuts at: 0 498 605 1788
Size: 498 107 1183
Fragments arranged by size:
1183 498 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 491 1788
Size: 491 1297
TatI w'GTAC_w
Cuts at: 0 232 801 1788
Size: 232 569 987
Fragments arranged by size:
987 569 232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 1788
Size: 804 984
Enzymes that do cut:
Acc65I AcuI AflIII ApaLI ApoI BanI BanII BbsI
BclI BfrBI Bme1580I BpmI BsaI BsaHI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI
Bsu36I BtgI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII HindIII KpnI MfeI MmeI
MscI MspA1I NcoI NdeI NsiI NspI PciI PflMI
PfoI PpuMI PsrI PstI SacI SacII SapI ScaI
SexAI SfcI SspI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraIII
DrdI EagI EciI EcoRI FalI FseI FspI FspAI
HaeII HpaI KasI MluI MslI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI