Restriction map for
pFN21AB3407
(Linear) (Six-base) MAPSORT of: KIBB3407.seq Check: 3989 from: 1 to: 3840
KIBB3407.orf
With 182 enzymes: SgfI *
October 14, 2014 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3685 3840
Size: 3685 155
AatII G_ACGT'C
Cuts at: 0 1790 3840
Size: 1790 2050
AccI GT'mk_AC
Cuts at: 0 1768 2073 3840
Size: 1768 305 1767
Fragments arranged by size:
1768 1767 305
Acc65I G'GTAC_C
Cuts at: 0 2775 3840
Size: 2775 1065
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1179 1264 2299 2714 3142 3840
Size: 782 397 85 1035 415 428 698
Fragments arranged by size:
1035 782 698 428 415 397 85
AflIII A'CryG_T
Cuts at: 0 625 3840
Size: 625 3215
AleI CACnn'nnGTG
Cuts at: 0 1747 3840
Size: 1747 2093
AlwNI CAG_nnn'CTG
Cuts at: 0 688 760 1289 3165 3195 3218 3246 3777
Size: 688 72 529 1876 30 23 28 531
Cuts at: 3777 3780 3840
Size: 3 60
Fragments arranged by size:
1876 688 531 529 72 60 30 28
23 3
ApaI G_GGCC'C
Cuts at: 0 1587 3840
Size: 1587 2253
ApaLI G'TGCA_C
Cuts at: 0 493 3840
Size: 493 3347
ApoI r'AATT_y
Cuts at: 0 249 837 1170 1177 1555 2109 3840
Size: 249 588 333 7 378 554 1731
Fragments arranged by size:
1731 588 554 378 333 249 7
AseI AT'TA_AT
Cuts at: 0 545 1015 3840
Size: 545 470 2825
Fragments arranged by size:
2825 545 470
BanI G'GyrC_C
Cuts at: 0 407 2775 2950 3840
Size: 407 2368 175 890
Fragments arranged by size:
2368 890 407 175
BanII G_rGCy'C
Cuts at: 0 1587 3359 3840
Size: 1587 1772 481
Fragments arranged by size:
1772 1587 481
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 429 1952 3840
Size: 332 97 1523 1888
Fragments arranged by size:
1888 1523 332 97
BciVI GTATCCnnnnn_n'
Cuts at: 0 3612 3840
Size: 3612 228
BclI T'GATC_A
Cuts at: 0 3029 3840
Size: 3029 811
BfrBI ATG'CAT
Cuts at: 0 922 3840
Size: 922 2918
BglII A'GATC_T
Cuts at: 0 258 1223 3840
Size: 258 965 2617
Fragments arranged by size:
2617 965 258
Bme1580I G_kGCm'C
Cuts at: 0 497 1148 1587 1632 3567 3840
Size: 497 651 439 45 1935 273
Fragments arranged by size:
1935 651 497 439 273 45
BmgBI CAC'GTC
Cuts at: 0 1673 3608 3840
Size: 1673 1935 232
Fragments arranged by size:
1935 1673 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 614 1581 3145 3840
Size: 614 967 1564 695
Fragments arranged by size:
1564 967 695 614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2365 3840
Size: 2365 1475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 770 2063 2360 2453 3193 3268 3840
Size: 340 430 1293 297 93 740 75 572
Fragments arranged by size:
1293 740 572 430 340 297 93 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2453 3840
Size: 2453 1387
BsaAI yAC'GTr
Cuts at: 0 628 1627 3840
Size: 628 999 2213
Fragments arranged by size:
2213 999 628
BsaHI Gr'CG_yC
Cuts at: 0 1787 3840
Size: 1787 2053
BsaWI w'CCGG_w
Cuts at: 0 2870 3141 3840
Size: 2870 271 699
Fragments arranged by size:
2870 699 271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1802 1832 2001 2031 2728 2758 3840
Size: 1802 30 169 30 697 30 1082
Fragments arranged by size:
1802 1082 697 169 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 41 1091 3840
Size: 41 1050 2749
Fragments arranged by size:
2749 1050 41
BseYI C'CCAG_C
Cuts at: 0 367 2677 3840
Size: 367 2310 1163
Fragments arranged by size:
2310 1163 367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 3840
Size: 1270 2570
BsiEI CG_ry'CG
Cuts at: 0 3142 3840
Size: 3142 698
BsiHKAI G_wGCw'C
Cuts at: 0 497 1303 3232 3333 3840
Size: 497 806 1929 101 507
Fragments arranged by size:
1929 806 507 497 101
BsmI GAATG_Cn'
Cuts at: 0 3720 3840
Size: 3720 120
Bsp1286I G_dGCh'C
Cuts at: 0 497 1148 1303 1587 1632 3232 3333 3359
Size: 497 651 155 284 45 1600 101 26
Cuts at: 3359 3567 3840
Size: 208 273
Fragments arranged by size:
1600 651 497 284 273 208 155 101
45 26
BspHI T'CATG_A
Cuts at: 0 200 311 1538 1833 1844 2903 3026 3840
Size: 200 111 1227 295 11 1059 123 814
Fragments arranged by size:
1227 1059 814 295 200 123 111 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2763 3685 3840
Size: 2763 922 155
Fragments arranged by size:
2763 922 155
BsrBI CCG'CTC
Cuts at: 0 3252 3840
Size: 3252 588
BsrDI GCAATG_nn'
Cuts at: 0 27 1619 2600 2846 3377 3389 3548 3840
Size: 27 1592 981 246 531 12 159 292
Fragments arranged by size:
1592 981 531 292 246 159 27 12
BssSI C'ACGA_G
Cuts at: 0 3560 3840
Size: 3560 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 928 3840
Size: 928 2912
BstBI TT'CG_AA
Cuts at: 0 1135 3840
Size: 1135 2705
BstXI CCAn_nnnn'nTGG
Cuts at: 0 187 1594 2961 3840
Size: 187 1407 1367 879
Fragments arranged by size:
1407 1367 879 187
BstYI r'GATC_y
Cuts at: 0 3 258 1223 1649 1975 2345 3840
Size: 3 255 965 426 326 370 1495
Fragments arranged by size:
1495 965 426 370 326 255 3
BstZ17I GTA'TAC
Cuts at: 0 2074 3840
Size: 2074 1766
BtgI C'CryG_G
Cuts at: 0 180 1487 2016 2954 2960 3840
Size: 180 1307 529 938 6 880
Fragments arranged by size:
1307 938 880 529 180 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1776 3840
Size: 1776 2064
BtsI GCAGTG_nn'
Cuts at: 0 1706 3219 3478 3840
Size: 1706 1513 259 362
Fragments arranged by size:
1706 1513 362 259
DraI TTT'AAA
Cuts at: 0 51 3840
Size: 51 3789
EaeI y'GGCC_r
Cuts at: 0 656 1470 2957 3534 3840
Size: 656 814 1487 577 306
Fragments arranged by size:
1487 814 656 577 306
EarI CTCTTCn'nnn_
Cuts at: 0 758 1132 1291 3220 3422 3840
Size: 758 374 159 1929 202 418
Fragments arranged by size:
1929 758 418 374 202 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 770 782 1179 1264 2063 2299 2360
Size: 340 430 12 397 85 799 236 61
Cuts at: 2360 2453 2714 3142 3193 3268 3840
Size: 93 261 428 51 75 572
Fragments arranged by size:
799 572 430 428 397 340 261 236
93 85 75 61 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2091 3840
Size: 2091 1749
EcoO109I rG'GnC_Cy
Cuts at: 0 1233 1583 3840
Size: 1233 350 2257
Fragments arranged by size:
2257 1233 350
EcoRI G'AATT_C
Cuts at: 0 1177 3840
Size: 1177 2663
EcoRV GAT'ATC
Cuts at: 0 231 291 3840
Size: 231 60 3549
Fragments arranged by size:
3549 231 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 60 3301 3840
Size: 60 3241 539
Fragments arranged by size:
3241 539 60
HaeII r_GCGC'y
Cuts at: 0 2678 3837 3840
Size: 2678 1159 3
Fragments arranged by size:
2678 1159 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 250 282 520 552 977 1009
Size: 182 32 36 32 238 32 425 32
Cuts at: 1009 1657 1689 1743 1775 1960 1992 2372 2404
Size: 648 32 54 32 185 32 380 32
Cuts at: 2404 3131 3163 3183 3215 3840
Size: 727 32 20 32 625
Fragments arranged by size:
727 648 625 425 380 238 185 182
54 36 32 32 32 32 32 32
32 32 32 32 20
HindIII A'AGCT_T
Cuts at: 0 2041 3840
Size: 2041 1799
KpnI G_GTAC'C
Cuts at: 0 2779 3840
Size: 2779 1061
MfeI C'AATT_G
Cuts at: 0 2588 3840
Size: 2588 1252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1014 1257 1551 1717 3840
Size: 613 401 243 294 166 2123
Fragments arranged by size:
2123 613 401 294 243 166
MscI TGG'CCA
Cuts at: 0 658 3536 3840
Size: 658 2878 304
Fragments arranged by size:
2878 658 304
MslI CAynn'nnrTG
Cuts at: 0 405 1411 1747 2756 2959 3025 3151 3840
Size: 405 1006 336 1009 203 66 126 689
Fragments arranged by size:
1009 1006 689 405 336 203 126 66
MspA1I CmG'CkG
Cuts at: 0 418 685 1314 1921 2938 3777 3840
Size: 418 267 629 607 1017 839 63
Fragments arranged by size:
1017 839 629 607 418 267 63
NcoI C'CATG_G
Cuts at: 0 180 1487 2954 3840
Size: 180 1307 1467 886
Fragments arranged by size:
1467 1307 886 180
NdeI CA'TA_TG
Cuts at: 0 2753 3840
Size: 2753 1087
NsiI A_TGCA'T
Cuts at: 0 924 3840
Size: 924 2916
NspI r_CATG'y
Cuts at: 0 3466 3840
Size: 3466 374
PflMI CCAn_nnn'nTGG
Cuts at: 0 3195 3246 3840
Size: 3195 51 594
Fragments arranged by size:
3195 594 51
PfoI T'CCnGG_A
Cuts at: 0 3245 3840
Size: 3245 595
PmlI CAC'GTG
Cuts at: 0 628 1627 3840
Size: 628 999 2213
Fragments arranged by size:
2213 999 628
PsiI TTA'TAA
Cuts at: 0 2158 3840
Size: 2158 1682
PspOMI G'GGCC_C
Cuts at: 0 1583 3840
Size: 1583 2257
PstI C_TGCA'G
Cuts at: 0 3376 3840
Size: 3376 464
PvuII CAG'CTG
Cuts at: 0 418 685 1314 1921 2938 3777 3840
Size: 418 267 629 607 1017 839 63
Fragments arranged by size:
1017 839 629 607 418 267 63
SapI GCTCTTCn'nnn_
Cuts at: 0 1291 3220 3840
Size: 1291 1929 620
Fragments arranged by size:
1929 1291 620
ScaI AGT'ACT
Cuts at: 0 992 3840
Size: 992 2848
SexAI A'CCwGG_T
Cuts at: 0 334 3840
Size: 334 3506
SfcI C'TryA_G
Cuts at: 0 893 1769 2483 3060 3372 3840
Size: 893 876 714 577 312 468
Fragments arranged by size:
893 876 714 577 468 312
SmlI C'TyrA_G
Cuts at: 0 2468 3840
Size: 2468 1372
SspI AAT'ATT
Cuts at: 0 888 976 1161 2192 3840
Size: 888 88 185 1031 1648
Fragments arranged by size:
1648 1031 888 185 88
StuI AGG'CCT
Cuts at: 0 2168 3074 3840
Size: 2168 906 766
Fragments arranged by size:
2168 906 766
StyI C'CwwG_G
Cuts at: 0 180 956 1487 1679 1949 2283 2954 3266
Size: 180 776 531 192 270 334 671 312
Cuts at: 3266 3840
Size: 574
Fragments arranged by size:
776 671 574 531 334 312 270 192
180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2848 3840
Size: 2848 992
TatI w'GTAC_w
Cuts at: 0 990 3840
Size: 990 2850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 1802 1834 2001 2033 3685 3717
Size: 1338 32 432 32 167 32 1652 32
Cuts at: 3717 3840
Size: 123
Fragments arranged by size:
1652 1338 432 167 123 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1344 1594 1677 2058 2087 3612 3840
Size: 1344 250 83 381 29 1525 228
Fragments arranged by size:
1525 1344 381 250 228 83 29
XmnI GAAnn'nnTTC
Cuts at: 0 1460 2292 3840
Size: 1460 832 1548
Fragments arranged by size:
1548 1460 832
ZraI GAC'GTC
Cuts at: 0 1788 3840
Size: 1788 2052
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI BanI BanII BbsI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HindIII KpnI MfeI MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PflMI
PfoI PmlI PsiI PspOMI PstI PvuII SapI ScaI
SexAI SfcI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AlfI AloI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BglI BlpI BmtI Bpu10I BsaI BsaBI BsiWI BsmBI
BspEI BsrFI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI
HincII HpaI KasI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI