Restriction map for
pFN21AB3389
(Linear) (Six-base) MAPSORT of: KIBB3389.seq Check: 7228 from: 1 to: 1356
KIBB3389.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 392 1039 1356
Size: 392 647 317
Fragments arranged by size:
647 392 317
AccI GT'mk_AC
Cuts at: 0 1064 1356
Size: 1064 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1356
Size: 1175 181
AlwNI CAG_nnn'CTG
Cuts at: 0 76 1107 1356
Size: 76 1031 249
Fragments arranged by size:
1031 249 76
ApaI G_GGCC'C
Cuts at: 0 366 1356
Size: 366 990
ApaLI G'TGCA_C
Cuts at: 0 581 1356
Size: 581 775
ApoI r'AATT_y
Cuts at: 0 39 500 625 1042 1135 1356
Size: 39 461 125 417 93 221
Fragments arranged by size:
461 417 221 125 93 39
AseI AT'TA_AT
Cuts at: 0 815 1356
Size: 815 541
AvaI C'yCGr_G
Cuts at: 0 172 1356
Size: 172 1184
BamHI G'GATC_C
Cuts at: 0 184 648 737 1356
Size: 184 464 89 619
Fragments arranged by size:
619 464 184 89
BanI G'GyrC_C
Cuts at: 0 167 201 306 1356
Size: 167 34 105 1050
Fragments arranged by size:
1050 167 105 34
BanII G_rGCy'C
Cuts at: 0 65 366 904 1356
Size: 65 301 538 452
Fragments arranged by size:
538 452 301 65
BbeI G_GCGC'C
Cuts at: 0 171 205 1356
Size: 171 34 1151
Fragments arranged by size:
1151 171 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 815 1356
Size: 815 541
BciVI GTATCCnnnnn_n'
Cuts at: 0 994 1088 1356
Size: 994 94 268
Fragments arranged by size:
994 268 94
Bme1580I G_kGCm'C
Cuts at: 0 311 366 585 1356
Size: 311 55 219 771
Fragments arranged by size:
771 311 219 55
BmgBI CAC'GTC
Cuts at: 0 285 1356
Size: 285 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 1356
Size: 405 951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 841 1356
Size: 93 748 515
Fragments arranged by size:
748 515 93
Bpu10I CC'TnA_GC
Cuts at: 0 367 1356
Size: 367 989
BsaAI yAC'GTr
Cuts at: 0 465 1356
Size: 465 891
BsaHI Gr'CG_yC
Cuts at: 0 168 202 1356
Size: 168 34 1154
Fragments arranged by size:
1154 168 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 1356
Size: 355 1001
BseYI C'CCAG_C
Cuts at: 0 141 150 669 1356
Size: 141 9 519 687
Fragments arranged by size:
687 519 141 9
BsiEI CG_ry'CG
Cuts at: 0 12 534 1356
Size: 12 522 822
Fragments arranged by size:
822 522 12
BsiHKAI G_wGCw'C
Cuts at: 0 585 1356
Size: 585 771
Bsp1286I G_dGCh'C
Cuts at: 0 65 311 366 585 904 1356
Size: 65 246 55 219 319 452
Fragments arranged by size:
452 319 246 219 65 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 1039 1356
Size: 392 647 317
Fragments arranged by size:
647 392 317
BsrBI CCG'CTC
Cuts at: 0 54 127 1356
Size: 54 73 1229
Fragments arranged by size:
1229 73 54
BsrGI T'GTAC_A
Cuts at: 0 972 1356
Size: 972 384
BssSI C'ACGA_G
Cuts at: 0 214 1356
Size: 214 1142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 1356
Size: 384 972
BstEII G'GTnAC_C
Cuts at: 0 411 1356
Size: 411 945
BstYI r'GATC_y
Cuts at: 0 184 648 737 1356
Size: 184 464 89 619
Fragments arranged by size:
619 464 184 89
Bsu36I CC'TnA_GG
Cuts at: 0 1328 1356
Size: 1328 28
BtgI C'CryG_G
Cuts at: 0 16 266 476 1356
Size: 16 250 210 880
Fragments arranged by size:
880 250 210 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 331 1356
Size: 331 1025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 780 815 1356
Size: 780 35 541
Fragments arranged by size:
780 541 35
DraI TTT'AAA
Cuts at: 0 1317 1356
Size: 1317 39
EaeI y'GGCC_r
Cuts at: 0 9 269 1356
Size: 9 260 1087
Fragments arranged by size:
1087 260 9
EagI C'GGCC_G
Cuts at: 0 9 1356
Size: 9 1347
EarI CTCTTCn'nnn_
Cuts at: 0 430 857 1189 1356
Size: 430 427 332 167
Fragments arranged by size:
430 427 332 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 240 1356
Size: 240 1116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 841 1175 1356
Size: 93 748 334 181
Fragments arranged by size:
748 334 181 93
EcoO109I rG'GnC_Cy
Cuts at: 0 362 363 399 1356
Size: 362 1 36 957
Fragments arranged by size:
957 362 36 1
EcoRV GAT'ATC
Cuts at: 0 837 1356
Size: 837 519
HaeII r_GCGC'y
Cuts at: 0 148 171 205 1356
Size: 148 23 34 1151
Fragments arranged by size:
1151 148 34 23
KasI G'GCGC_C
Cuts at: 0 167 201 1356
Size: 167 34 1155
Fragments arranged by size:
1155 167 34
MfeI C'AATT_G
Cuts at: 0 1292 1356
Size: 1292 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 917 1356
Size: 53 864 439
Fragments arranged by size:
864 439 53
MscI TGG'CCA
Cuts at: 0 271 1356
Size: 271 1085
MslI CAynn'nnrTG
Cuts at: 0 578 1037 1356
Size: 578 459 319
Fragments arranged by size:
578 459 319
MspA1I CmG'CkG
Cuts at: 0 18 73 1356
Size: 18 55 1283
Fragments arranged by size:
1283 55 18
NarI GG'CG_CC
Cuts at: 0 168 202 1356
Size: 168 34 1154
Fragments arranged by size:
1154 168 34
NcoI C'CATG_G
Cuts at: 0 266 476 1356
Size: 266 210 880
Fragments arranged by size:
880 266 210
NdeI CA'TA_TG
Cuts at: 0 1017 1356
Size: 1017 339
NotI GC'GGCC_GC
Cuts at: 0 9 1356
Size: 9 1347
NspI r_CATG'y
Cuts at: 0 661 1356
Size: 661 695
PfoI T'CCnGG_A
Cuts at: 0 857 1356
Size: 857 499
PpuMI rG'GwC_Cy
Cuts at: 0 399 1356
Size: 399 957
PshAI GACnn'nnGTC
Cuts at: 0 1070 1356
Size: 1070 286
PspOMI G'GGCC_C
Cuts at: 0 362 1356
Size: 362 994
PstI C_TGCA'G
Cuts at: 0 422 1356
Size: 422 934
PvuI CG_AT'CG
Cuts at: 0 534 1356
Size: 534 822
PvuII CAG'CTG
Cuts at: 0 73 1356
Size: 73 1283
SacII CC_GC'GG
Cuts at: 0 19 1356
Size: 19 1337
SanDI GG'GwC_CC
Cuts at: 0 399 1356
Size: 399 957
SapI GCTCTTCn'nnn_
Cuts at: 0 430 1356
Size: 430 926
ScaI AGT'ACT
Cuts at: 0 1312 1356
Size: 1312 44
SfcI C'TryA_G
Cuts at: 0 418 1068 1093 1356
Size: 418 650 25 263
Fragments arranged by size:
650 418 263 25
SfoI GGC'GCC
Cuts at: 0 169 203 1356
Size: 169 34 1153
Fragments arranged by size:
1153 169 34
SmaI CCC'GGG
Cuts at: 0 174 1356
Size: 174 1182
SspI AAT'ATT
Cuts at: 0 994 1356
Size: 994 362
StyI C'CwwG_G
Cuts at: 0 266 476 1281 1356
Size: 266 210 805 75
Fragments arranged by size:
805 266 210 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 1356
Size: 566 790
TatI w'GTAC_w
Cuts at: 0 972 1310 1356
Size: 972 338 46
Fragments arranged by size:
972 338 46
XmaI C'CCGG_G
Cuts at: 0 172 1356
Size: 172 1184
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BciVI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI CspCI DraI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRV HaeII KasI
MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI
NotI NspI PfoI PpuMI PshAI PspOMI PstI PvuI
PvuII SacII SanDI SapI ScaI SfcI SfoI SmaI
SspI StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AsiSI AvrII BaeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmtI BplI
BpuEI BsaI BsaBI BsaWI BsaXI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BssHII BstBI BstXI
BstZ17I BtsI ClaI DraIII DrdI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MluI NaeI NgoMIV NheI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI RsrII SacI SalI SbfI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI