Restriction map for
pFN21AB3278
(Linear) (Six-base) MAPSORT of: KIBB3278.seq Check: 1698 from: 1 to: 2727
KIBB3278.orf
With 182 enzymes: SgfI *
October 13, 2009 15:05 ..
AccI GT'mk_AC
Cuts at: 0 260 470 692 2727
Size: 260 210 222 2035
Fragments arranged by size:
2035 260 222 210
Acc65I G'GTAC_C
Cuts at: 0 1172 2727
Size: 1172 1555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1731 2727
Size: 1213 518 996
Fragments arranged by size:
1213 996 518
AflIII A'CryG_T
Cuts at: 0 734 1322 2567 2727
Size: 734 588 1245 160
Fragments arranged by size:
1245 734 588 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 1099 1399 2299 2540 2727
Size: 367 732 300 900 241 187
Fragments arranged by size:
900 732 367 300 241 187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 2727
Size: 1024 32 1671
Fragments arranged by size:
1671 1024 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1611 2300 2727
Size: 1611 689 427
Fragments arranged by size:
1611 689 427
ApaI G_GGCC'C
Cuts at: 0 1316 2628 2727
Size: 1316 1312 99
Fragments arranged by size:
1316 1312 99
ApaLI G'TGCA_C
Cuts at: 0 874 1426 1708 2727
Size: 874 552 282 1019
Fragments arranged by size:
1019 874 552 282
AvaI C'yCGr_G
Cuts at: 0 647 762 799 886 1166 2265 2727
Size: 647 115 37 87 280 1099 462
Fragments arranged by size:
1099 647 462 280 115 87 37
BanI G'GyrC_C
Cuts at: 0 1105 1172 1350 1542 1864 2081 2616 2727
Size: 1105 67 178 192 322 217 535 111
Fragments arranged by size:
1105 535 322 217 192 178 111 67
BanII G_rGCy'C
Cuts at: 0 727 884 1220 1316 2062 2628 2727
Size: 727 157 336 96 746 566 99
Fragments arranged by size:
746 727 566 336 157 99 96
BbeI G_GCGC'C
Cuts at: 0 1546 1868 2085 2727
Size: 1546 322 217 642
Fragments arranged by size:
1546 642 322 217
BbsI GAAGACnn'nnnn_
Cuts at: 0 952 971 2363 2439 2727
Size: 952 19 1392 76 288
Fragments arranged by size:
1392 952 288 76 19
BbvCI CC'TCA_GC
Cuts at: 0 2014 2727
Size: 2014 713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 695 2727
Size: 661 34 2032
Fragments arranged by size:
2032 661 34
BclI T'GATC_A
Cuts at: 0 1922 2727
Size: 1922 805
BglI GCCn_nnn'nGGC
Cuts at: 0 1757 1951 2155 2727
Size: 1757 194 204 572
Fragments arranged by size:
1757 572 204 194
BglII A'GATC_T
Cuts at: 0 758 2727
Size: 758 1969
BlpI GC'TnA_GC
Cuts at: 0 97 1516 2095 2727
Size: 97 1419 579 632
Fragments arranged by size:
1419 632 579 97
Bme1580I G_kGCm'C
Cuts at: 0 878 1316 1430 1712 2621 2628 2727
Size: 878 438 114 282 909 7 99
Fragments arranged by size:
909 878 438 282 114 99 7
BmgBI CAC'GTC
Cuts at: 0 1113 1325 2372 2727
Size: 1113 212 1047 355
Fragments arranged by size:
1113 1047 355 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 1282 2727
Size: 1282 1445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 559 571 661 2227 2267 2717 2727
Size: 234 325 12 90 1566 40 450 10
Fragments arranged by size:
1566 450 325 234 90 40 12 10
Bpu10I CC'TnA_GC
Cuts at: 0 403 2014 2727
Size: 403 1611 713
Fragments arranged by size:
1611 713 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2314 2727
Size: 2314 413
BsaAI yAC'GTr
Cuts at: 0 735 1713 2727
Size: 735 978 1014
Fragments arranged by size:
1014 978 735
BsaBI GATnn'nnATC
Cuts at: 0 2494 2727
Size: 2494 233
BsaHI Gr'CG_yC
Cuts at: 0 848 1543 1835 1865 2082 2727
Size: 848 695 292 30 217 645
Fragments arranged by size:
848 695 645 292 217 30
BsaWI w'CCGG_w
Cuts at: 0 1034 2727
Size: 1034 1693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 547 577 2727
Size: 232 30 285 30 2150
Fragments arranged by size:
2150 285 232 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 874 1279 1795 1798 1801 1804 1996
Size: 568 306 405 516 3 3 3 192
Cuts at: 1996 2636 2727
Size: 640 91
Fragments arranged by size:
640 568 516 405 306 192 91 3
3 3
BseYI C'CCAG_C
Cuts at: 0 354 2461 2727
Size: 354 2107 266
Fragments arranged by size:
2107 354 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1290 2727
Size: 1290 1437
BsiEI CG_ry'CG
Cuts at: 0 1761 1978 2727
Size: 1761 217 749
Fragments arranged by size:
1761 749 217
BsiHKAI G_wGCw'C
Cuts at: 0 7 103 878 884 1430 1648 1681 1712
Size: 7 96 775 6 546 218 33 31
Cuts at: 1712 2062 2101 2727
Size: 350 39 626
Fragments arranged by size:
775 626 546 350 218 96 39 33
31 7 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 535 2319 2727
Size: 535 1784 408
Fragments arranged by size:
1784 535 408
Bsp1286I G_dGCh'C
Cuts at: 0 7 103 727 878 884 1220 1316 1430
Size: 7 96 624 151 6 336 96 114
Cuts at: 1430 1648 1681 1712 2062 2101 2621 2628 2727
Size: 218 33 31 350 39 520 7 99
Fragments arranged by size:
624 520 350 336 218 151 114 99
96 96 39 33 31 7 7 6
BspHI T'CATG_A
Cuts at: 0 1058 2727
Size: 1058 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1073 1391 1742 2577 2591 2727
Size: 1073 318 351 835 14 136
Fragments arranged by size:
1073 835 351 318 136 14
BsrBI CCG'CTC
Cuts at: 0 957 1464 2264 2727
Size: 957 507 800 463
Fragments arranged by size:
957 800 507 463
BsrFI r'CCGG_y
Cuts at: 0 331 1760 1909 1973 2108 2727
Size: 331 1429 149 64 135 619
Fragments arranged by size:
1429 619 331 149 135 64
BssHII G'CGCG_C
Cuts at: 0 1566 1568 1885 2727
Size: 1566 2 317 842
Fragments arranged by size:
1566 842 317 2
BssSI C'ACGA_G
Cuts at: 0 112 511 877 2338 2727
Size: 112 399 366 1461 389
Fragments arranged by size:
1461 399 389 366 112
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 2344 2727
Size: 219 2125 383
Fragments arranged by size:
2125 383 219
BstYI r'GATC_y
Cuts at: 0 758 2480 2727
Size: 758 1722 247
Fragments arranged by size:
1722 758 247
BstZ17I GTA'TAC
Cuts at: 0 693 2727
Size: 693 2034
BtgI C'CryG_G
Cuts at: 0 913 2045 2063 2637 2663 2709 2727
Size: 913 1132 18 574 26 46 18
Fragments arranged by size:
1132 913 574 46 26 18 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 721 2727
Size: 721 2006
DraIII CAC_nnn'GTG
Cuts at: 0 1350 1434 1685 1716 2727
Size: 1350 84 251 31 1011
Fragments arranged by size:
1350 1011 251 84 31
EaeI y'GGCC_r
Cuts at: 0 1141 1718 1758 1814 1975 2438 2474 2634
Size: 1141 577 40 56 161 463 36 160
Cuts at: 2634 2712 2727
Size: 78 15
Fragments arranged by size:
1141 577 463 161 160 78 56 40
36 15
EagI C'GGCC_G
Cuts at: 0 1758 1975 2727
Size: 1758 217 752
Fragments arranged by size:
1758 752 217
EarI CTCTTCn'nnn_
Cuts at: 0 964 2727
Size: 964 1763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1163 2158 2589 2727
Size: 1163 995 431 138
Fragments arranged by size:
1163 995 431 138
EcoICRI GAG'CTC
Cuts at: 0 882 2060 2727
Size: 882 1178 667
Fragments arranged by size:
1178 882 667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 559 571 661 1213 1731 2227 2267
Size: 234 325 12 90 552 518 496 40
Cuts at: 2267 2717 2727
Size: 450 10
Fragments arranged by size:
552 518 496 450 325 234 90 40
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 2727
Size: 889 1838
EcoO109I rG'GnC_Cy
Cuts at: 0 240 1608 2624 2727
Size: 240 1368 1016 103
Fragments arranged by size:
1368 1016 240 103
EcoRV GAT'ATC
Cuts at: 0 2070 2727
Size: 2070 657
FspI TGC'GCA
Cuts at: 0 415 2287 2727
Size: 415 1872 440
Fragments arranged by size:
1872 440 415
HaeII r_GCGC'y
Cuts at: 0 1546 1868 2085 2727
Size: 1546 322 217 642
Fragments arranged by size:
1546 642 322 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 547 579 2727
Size: 232 32 283 32 2148
Fragments arranged by size:
2148 283 232 32 32
HincII GTy'rAC
Cuts at: 0 81 1278 2565 2727
Size: 81 1197 1287 162
Fragments arranged by size:
1287 1197 162 81
HindIII A'AGCT_T
Cuts at: 0 1875 2727
Size: 1875 852
HpaI GTT'AAC
Cuts at: 0 2565 2727
Size: 2565 162
KasI G'GCGC_C
Cuts at: 0 1542 1864 2081 2727
Size: 1542 322 217 646
Fragments arranged by size:
1542 646 322 217
KpnI G_GTAC'C
Cuts at: 0 1176 2727
Size: 1176 1551
MscI TGG'CCA
Cuts at: 0 1143 2636 2714 2727
Size: 1143 1493 78 13
Fragments arranged by size:
1493 1143 78 13
MslI CAynn'nnrTG
Cuts at: 0 217 349 370 1537 2281 2342 2522 2727
Size: 217 132 21 1167 744 61 180 205
Fragments arranged by size:
1167 744 217 205 180 132 61 21
MspA1I CmG'CkG
Cuts at: 0 476 915 1520 2547 2727
Size: 476 439 605 1027 180
Fragments arranged by size:
1027 605 476 439 180
NaeI GCC'GGC
Cuts at: 0 333 1911 2727
Size: 333 1578 816
Fragments arranged by size:
1578 816 333
NarI GG'CG_CC
Cuts at: 0 1543 1865 2082 2727
Size: 1543 322 217 645
Fragments arranged by size:
1543 645 322 217
NcoI C'CATG_G
Cuts at: 0 2709 2727
Size: 2709 18
NdeI CA'TA_TG
Cuts at: 0 2114 2727
Size: 2114 613
NgoMIV G'CCGG_C
Cuts at: 0 331 1909 2727
Size: 331 1578 818
Fragments arranged by size:
1578 818 331
NspI r_CATG'y
Cuts at: 0 375 1259 2182 2286 2727
Size: 375 884 923 104 441
Fragments arranged by size:
923 884 441 375 104
PasI CC'CwG_GG
Cuts at: 0 595 2621 2727
Size: 595 2026 106
Fragments arranged by size:
2026 595 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 449 941 2727
Size: 449 492 1786
Fragments arranged by size:
1786 492 449
PfoI T'CCnGG_A
Cuts at: 0 163 2727
Size: 163 2564
PmlI CAC'GTG
Cuts at: 0 1713 2727
Size: 1713 1014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 2727
Size: 106 32 2589
Fragments arranged by size:
2589 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 240 2727
Size: 240 2487
PspOMI G'GGCC_C
Cuts at: 0 1312 2624 2727
Size: 1312 1312 103
Fragments arranged by size:
1312 1312 103
PspXI vC'TCGA_Gb
Cuts at: 0 1166 2727
Size: 1166 1561
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1402 1434 2727
Size: 1402 32 1293
Fragments arranged by size:
1402 1293 32
PstI C_TGCA'G
Cuts at: 0 587 809 1070 2588 2727
Size: 587 222 261 1518 139
Fragments arranged by size:
1518 587 261 222 139
PvuII CAG'CTG
Cuts at: 0 476 2547 2727
Size: 476 2071 180
Fragments arranged by size:
2071 476 180
SacI G_AGCT'C
Cuts at: 0 884 2062 2727
Size: 884 1178 665
Fragments arranged by size:
1178 884 665
SacII CC_GC'GG
Cuts at: 0 916 2727
Size: 916 1811
SapI GCTCTTCn'nnn_
Cuts at: 0 964 2727
Size: 964 1763
SbfI CC_TGCA'GG
Cuts at: 0 2588 2727
Size: 2588 139
SexAI A'CCwGG_T
Cuts at: 0 1430 2234 2727
Size: 1430 804 493
Fragments arranged by size:
1430 804 493
SfcI C'TryA_G
Cuts at: 0 471 583 805 1050 1066 2584 2727
Size: 471 112 222 245 16 1518 143
Fragments arranged by size:
1518 471 245 222 143 112 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2155 2727
Size: 2155 572
SfoI GGC'GCC
Cuts at: 0 1544 1866 2083 2727
Size: 1544 322 217 644
Fragments arranged by size:
1544 644 322 217
SmaI CCC'GGG
Cuts at: 0 649 2727
Size: 649 2078
SmlI C'TyrA_G
Cuts at: 0 886 1166 2329 2727
Size: 886 280 1163 398
Fragments arranged by size:
1163 886 398 280
SphI G_CATG'C
Cuts at: 0 2182 2286 2727
Size: 2182 104 441
Fragments arranged by size:
2182 441 104
StuI AGG'CCT
Cuts at: 0 2218 2612 2727
Size: 2218 394 115
Fragments arranged by size:
2218 394 115
StyI C'CwwG_G
Cuts at: 0 566 2709 2727
Size: 566 2143 18
Fragments arranged by size:
2143 566 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1123 2727
Size: 1123 1604
TatI w'GTAC_w
Cuts at: 0 1574 2727
Size: 1574 1153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2356 2388 2727
Size: 2356 32 339
Fragments arranged by size:
2356 339 32
Tth111I GACn'n_nGTC
Cuts at: 0 1288 1806 2727
Size: 1288 518 921
Fragments arranged by size:
1288 921 518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1926 2727
Size: 1926 801
XhoI C'TCGA_G
Cuts at: 0 886 1166 2727
Size: 886 280 1561
Fragments arranged by size:
1561 886 280
XmaI C'CCGG_G
Cuts at: 0 647 2727
Size: 647 2080
XmnI GAAnn'nnTTC
Cuts at: 0 2347 2727
Size: 2347 380
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BssSI BstXI BstYI BstZ17I BtgI
BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI
SacII SapI SbfI SexAI SfcI SfiI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BfrBI BmtI BplI BsaI BsiWI BsmI BspEI BsrDI
BsrGI BstAPI BstBI BstEII Bsu36I BtsI ClaI CspCI
DraI DrdI EcoRI FalI FseI FspAI MfeI MluI
MmeI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PvuI RsrII SalI SanDI ScaI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI