(Linear) (Six-base) MAPSORT of: KIBB3073.seq  Check: 2951  from: 1  to: 396



KIBB3073.orf

 With 182 enzymes: SgfI * 

                        November 15, 2011 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      84     396 
   Size:         84     312
 
AhdI GACnn_n'nnGTC

Cuts at:      0      38     396 
   Size:         38     358
 
ApoI r'AATT_y

Cuts at:      0     309     396 
   Size:        309      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     131     161     396 
   Size:        131      30     235

  Fragments arranged by size: 

                 235     131      30
 
BseYI C'CCAG_C

Cuts at:      0     198     396 
   Size:        198     198
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      84     396 
   Size:         84     312
 
BtsI GCAGTG_nn'

Cuts at:      0     221     396 
   Size:        221     175
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     148     396 
   Size:        148     248
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     396 
   Size:        200     196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56     396 
   Size:         24      32     340

  Fragments arranged by size: 

                 340      32      24
 
MspA1I CmG'CkG

Cuts at:      0     198     396 
   Size:        198     198
 
PstI C_TGCA'G

Cuts at:      0     228     396 
   Size:        228     168
 
PvuII CAG'CTG

Cuts at:      0     198     396 
   Size:        198     198
 
SfcI C'TryA_G

Cuts at:      0     224     396 
   Size:        224     172
 
TatI w'GTAC_w

Cuts at:      0      56     396 
   Size:         56     340
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     131     163     396 
   Size:        131      32     233

  Fragments arranged by size: 

                 233     131      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     396 
   Size:         93     303
 

 Enzymes that do cut:

     AarI     AhdI     ApoI    BsaXI    BseYI    BspMI     BtsI     DrdI
     EarI    Hin4I   MspA1I     PstI    PvuII     SfcI     TatI     TstI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI