Restriction map for
pFN21AB3035
(Linear) (Six-base) MAPSORT of: KIBB3035.seq Check: 6413 from: 1 to: 1863
KIBB3035.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 732 1157 1431 1863
Size: 732 425 274 432
Fragments arranged by size:
732 432 425 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 871 1552 1863
Size: 31 840 681 311
Fragments arranged by size:
840 681 311 31
AflIII A'CryG_T
Cuts at: 0 772 1863
Size: 772 1091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1165 1863
Size: 1165 698
AlwNI CAG_nnn'CTG
Cuts at: 0 846 849 1863
Size: 846 3 1014
Fragments arranged by size:
1014 846 3
ApaI G_GGCC'C
Cuts at: 0 1111 1179 1404 1863
Size: 1111 68 225 459
Fragments arranged by size:
1111 459 225 68
ApaLI G'TGCA_C
Cuts at: 0 1418 1863
Size: 1418 445
AvaI C'yCGr_G
Cuts at: 0 1104 1170 1397 1709 1863
Size: 1104 66 227 312 154
Fragments arranged by size:
1104 312 227 154 66
BanI G'GyrC_C
Cuts at: 0 516 574 1863
Size: 516 58 1289
Fragments arranged by size:
1289 516 58
BanII G_rGCy'C
Cuts at: 0 1044 1111 1169 1179 1404 1449 1690 1863
Size: 1044 67 58 10 225 45 241 173
Fragments arranged by size:
1044 241 225 173 67 58 45 10
BbvCI CC'TCA_GC
Cuts at: 0 1132 1385 1863
Size: 1132 253 478
Fragments arranged by size:
1132 478 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1570 1604 1714 1748 1863
Size: 1570 34 110 34 115
Fragments arranged by size:
1570 115 110 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 106 932 1158 1220 1311 1863
Size: 106 826 226 62 91 552
Fragments arranged by size:
826 552 226 106 91 62
BlpI GC'TnA_GC
Cuts at: 0 668 1043 1863
Size: 668 375 820
Fragments arranged by size:
820 668 375
Bme1580I G_kGCm'C
Cuts at: 0 102 185 521 1076 1111 1179 1302 1404
Size: 102 83 336 555 35 68 123 102
Cuts at: 1404 1422 1863
Size: 18 441
Fragments arranged by size:
555 441 336 123 102 102 83 68
35 18
BmgBI CAC'GTC
Cuts at: 0 1502 1508 1863
Size: 1502 6 355
Fragments arranged by size:
1502 355 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 473 721 906 1122 1183 1863
Size: 285 188 248 185 216 61 680
Fragments arranged by size:
680 285 248 216 188 185 61
Bpu10I CC'TnA_GC
Cuts at: 0 761 1132 1385 1863
Size: 761 371 253 478
Fragments arranged by size:
761 478 371 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 902 1863
Size: 902 961
BsaAI yAC'GTr
Cuts at: 0 81 773 1863
Size: 81 692 1090
Fragments arranged by size:
1090 692 81
BsaHI Gr'CG_yC
Cuts at: 0 1516 1661 1863
Size: 1516 145 202
Fragments arranged by size:
1516 202 145
BsaWI w'CCGG_w
Cuts at: 0 866 1863
Size: 866 997
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 417 447 1863
Size: 417 30 1416
Fragments arranged by size:
1416 417 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 430 724 1539 1708 1863
Size: 427 3 294 815 169 155
Fragments arranged by size:
815 427 294 169 155 3
BseYI C'CCAG_C
Cuts at: 0 197 1065 1254 1301 1306 1403 1449 1652
Size: 197 868 189 47 5 97 46 203
Cuts at: 1652 1664 1863
Size: 12 199
Fragments arranged by size:
868 203 199 197 189 97 47 46
12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 826 1600 1777 1863
Size: 619 207 774 177 86
Fragments arranged by size:
774 619 207 177 86
BsiEI CG_ry'CG
Cuts at: 0 255 1224 1863
Size: 255 969 639
Fragments arranged by size:
969 639 255
BsiHKAI G_wGCw'C
Cuts at: 0 863 892 1044 1238 1422 1863
Size: 863 29 152 194 184 441
Fragments arranged by size:
863 441 194 184 152 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 1507 1863
Size: 412 1095 356
Fragments arranged by size:
1095 412 356
Bsp1286I G_dGCh'C
Cuts at: 0 102 185 521 863 892 1044 1076 1111
Size: 102 83 336 342 29 152 32 35
Cuts at: 1111 1169 1179 1238 1302 1404 1422 1449 1690
Size: 58 10 59 64 102 18 27 241
Cuts at: 1690 1863
Size: 173
Fragments arranged by size:
342 336 241 173 152 102 102 83
64 59 58 35 32 29 27 18
10
BspEI T'CCGG_A
Cuts at: 0 866 1863
Size: 866 997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 732 1157 1431 1486 1622 1799 1863
Size: 732 425 274 55 136 177 64
Fragments arranged by size:
732 425 274 177 136 64 55
BsrDI GCAATG_nn'
Cuts at: 0 1209 1863
Size: 1209 654
BsrGI T'GTAC_A
Cuts at: 0 775 794 1863
Size: 775 19 1069
Fragments arranged by size:
1069 775 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1722 1863
Size: 1722 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 1863
Size: 549 1314
Bsu36I CC'TnA_GG
Cuts at: 0 103 1863
Size: 103 1760
BtgI C'CryG_G
Cuts at: 0 1156 1863
Size: 1156 707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1809 1863
Size: 1809 54
BtsI GCAGTG_nn'
Cuts at: 0 778 1863
Size: 778 1085
DraIII CAC_nnn'GTG
Cuts at: 0 359 1863
Size: 359 1504
EaeI y'GGCC_r
Cuts at: 0 252 746 1080 1190 1221 1863
Size: 252 494 334 110 31 642
Fragments arranged by size:
642 494 334 252 110 31
EagI C'GGCC_G
Cuts at: 0 252 1221 1863
Size: 252 969 642
Fragments arranged by size:
969 642 252
EarI CTCTTCn'nnn_
Cuts at: 0 562 1557 1863
Size: 562 995 306
Fragments arranged by size:
995 562 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 424 724 1863
Size: 424 300 1139
Fragments arranged by size:
1139 424 300
EcoICRI GAG'CTC
Cuts at: 0 1042 1863
Size: 1042 821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 285 473 721 871 906 1122 1183
Size: 31 254 188 248 150 35 216 61
Cuts at: 1183 1552 1863
Size: 369 311
Fragments arranged by size:
369 311 254 248 216 188 150 61
35 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 739 1863
Size: 703 36 1124
Fragments arranged by size:
1124 703 36
EcoO109I rG'GnC_Cy
Cuts at: 0 107 614 1175 1863
Size: 107 507 561 688
Fragments arranged by size:
688 561 507 107
EcoRV GAT'ATC
Cuts at: 0 1022 1863
Size: 1022 841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1599 1863
Size: 1599 264
HaeII r_GCGC'y
Cuts at: 0 10 948 1566 1863
Size: 10 938 618 297
Fragments arranged by size:
938 618 297 10
HincII GTy'rAC
Cuts at: 0 1056 1863
Size: 1056 807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1490 1863
Size: 269 1221 373
Fragments arranged by size:
1221 373 269
MscI TGG'CCA
Cuts at: 0 748 1863
Size: 748 1115
MspA1I CmG'CkG
Cuts at: 0 599 846 1158 1220 1781 1842 1848 1863
Size: 599 247 312 62 561 61 6 15
Fragments arranged by size:
599 561 312 247 62 61 15 6
NspI r_CATG'y
Cuts at: 0 795 1863
Size: 795 1068
PasI CC'CwG_GG
Cuts at: 0 267 1683 1863
Size: 267 1416 180
Fragments arranged by size:
1416 267 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 744 1863
Size: 744 1119
PfoI T'CCnGG_A
Cuts at: 0 523 1863
Size: 523 1340
PmlI CAC'GTG
Cuts at: 0 81 773 1863
Size: 81 692 1090
Fragments arranged by size:
1090 692 81
PspOMI G'GGCC_C
Cuts at: 0 1107 1175 1400 1863
Size: 1107 68 225 463
Fragments arranged by size:
1107 463 225 68
PspXI vC'TCGA_Gb
Cuts at: 0 1709 1863
Size: 1709 154
PstI C_TGCA'G
Cuts at: 0 689 785 977 1483 1853 1863
Size: 689 96 192 506 370 10
Fragments arranged by size:
689 506 370 192 96 10
PvuII CAG'CTG
Cuts at: 0 846 1848 1863
Size: 846 1002 15
Fragments arranged by size:
1002 846 15
SacI G_AGCT'C
Cuts at: 0 1044 1863
Size: 1044 819
SacII CC_GC'GG
Cuts at: 0 1159 1863
Size: 1159 704
SfcI C'TryA_G
Cuts at: 0 685 781 973 1479 1761 1849 1863
Size: 685 96 192 506 282 88 14
Fragments arranged by size:
685 506 282 192 96 88 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1220 1863
Size: 1220 643
SmaI CCC'GGG
Cuts at: 0 1106 1399 1863
Size: 1106 293 464
Fragments arranged by size:
1106 464 293
SmlI C'TyrA_G
Cuts at: 0 1709 1863
Size: 1709 154
StuI AGG'CCT
Cuts at: 0 1090 1863
Size: 1090 773
StyI C'CwwG_G
Cuts at: 0 177 903 1863
Size: 177 726 960
Fragments arranged by size:
960 726 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 641 1444 1666 1863
Size: 641 803 222 197
Fragments arranged by size:
803 641 222 197
TatI w'GTAC_w
Cuts at: 0 775 794 1595 1863
Size: 775 19 801 268
Fragments arranged by size:
801 775 268 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 417 449 558 590 757 789 1863
Size: 417 32 109 32 167 32 1074
Fragments arranged by size:
1074 417 167 109 32 32 32
XhoI C'TCGA_G
Cuts at: 0 1709 1863
Size: 1709 154
XmaI C'CCGG_G
Cuts at: 0 1104 1397 1863
Size: 1104 293 466
Fragments arranged by size:
1104 466 293
XmnI GAAnn'nnTTC
Cuts at: 0 1582 1863
Size: 1582 281
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbvCI BcgI BglI BlpI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrGI BstAPI BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII HincII MmeI MscI MspA1I
NspI PasI PflMI PfoI PmlI PspOMI PspXI PstI
PvuII SacI SacII SfcI SfiI SmaI SmlI StuI
StyI TaqII TatI TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BglII BmrI
BmtI BplI BpuEI BsaBI BsiWI BsmI BspHI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraI DrdI EcoRI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI ZraI