Restriction map for
pFN21AB3014
(Linear) (Six-base) MAPSORT of: KIBB3014.seq Check: 97 from: 1 to: 3030
KIBB3014.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 748 1664 2330 2861 2947 3030
Size: 410 338 916 666 531 86 83
Fragments arranged by size:
916 666 531 410 338 86 83
AflIII A'CryG_T
Cuts at: 0 1727 2019 2322 3030
Size: 1727 292 303 708
Fragments arranged by size:
1727 708 303 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 988 3030
Size: 988 2042
AlwNI CAG_nnn'CTG
Cuts at: 0 115 1114 1927 2247 2542 2815 3030
Size: 115 999 813 320 295 273 215
Fragments arranged by size:
999 813 320 295 273 215 115
ApaLI G'TGCA_C
Cuts at: 0 2820 3030
Size: 2820 210
ApoI r'AATT_y
Cuts at: 0 202 1358 2617 2690 3030
Size: 202 1156 1259 73 340
Fragments arranged by size:
1259 1156 340 202 73
AseI AT'TA_AT
Cuts at: 0 2588 3030
Size: 2588 442
AvaI C'yCGr_G
Cuts at: 0 8 2483 3030
Size: 8 2475 547
Fragments arranged by size:
2475 547 8
BamHI G'GATC_C
Cuts at: 0 2123 3030
Size: 2123 907
BanI G'GyrC_C
Cuts at: 0 1771 3030
Size: 1771 1259
BanII G_rGCy'C
Cuts at: 0 1592 3030
Size: 1592 1438
BbsI GAAGACnn'nnnn_
Cuts at: 0 1846 2127 3030
Size: 1846 281 903
Fragments arranged by size:
1846 903 281
BciVI GTATCCnnnnn_n'
Cuts at: 0 1742 3030
Size: 1742 1288
BglI GCCn_nnn'nGGC
Cuts at: 0 541 981 2428 3030
Size: 541 440 1447 602
Fragments arranged by size:
1447 602 541 440
BglII A'GATC_T
Cuts at: 0 3008 3030
Size: 3008 22
Bme1580I G_kGCm'C
Cuts at: 0 2824 3030
Size: 2824 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 2825 3030
Size: 2825 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 685 1055 1114 1270 1340 1760 1891
Size: 569 116 370 59 156 70 420 131
Cuts at: 1891 1904 2139 3030
Size: 13 235 891
Fragments arranged by size:
891 569 420 370 235 156 131 116
70 59 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1219 3030
Size: 1064 155 1811
Fragments arranged by size:
1811 1064 155
BsaAI yAC'GTr
Cuts at: 0 2323 3030
Size: 2323 707
BsaWI w'CCGG_w
Cuts at: 0 2800 3030
Size: 2800 230
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 2795 2825 3030
Size: 575 30 2190 30 205
Fragments arranged by size:
2190 575 205 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1905 3030
Size: 1905 1125
BseYI C'CCAG_C
Cuts at: 0 238 639 1508 1756 2542 3030
Size: 238 401 869 248 786 488
Fragments arranged by size:
869 786 488 401 248 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 948 2486 3030
Size: 437 511 1538 544
Fragments arranged by size:
1538 544 511 437
BsiHKAI G_wGCw'C
Cuts at: 0 1695 2824 3030
Size: 1695 1129 206
Fragments arranged by size:
1695 1129 206
Bsp1286I G_dGCh'C
Cuts at: 0 1592 1695 2824 3030
Size: 1592 103 1129 206
Fragments arranged by size:
1592 1129 206 103
BspHI T'CATG_A
Cuts at: 0 2684 3030
Size: 2684 346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2468 3030
Size: 2468 562
BsrDI GCAATG_nn'
Cuts at: 0 280 1559 1869 3030
Size: 280 1279 310 1161
Fragments arranged by size:
1279 1161 310 280
BsrGI T'GTAC_A
Cuts at: 0 510 2826 2985 3030
Size: 510 2316 159 45
Fragments arranged by size:
2316 510 159 45
BssSI C'ACGA_G
Cuts at: 0 2303 3030
Size: 2303 727
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 119 1114 3030
Size: 115 4 995 1916
Fragments arranged by size:
1916 995 115 4
BstEII G'GTnAC_C
Cuts at: 0 1939 3030
Size: 1939 1091
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2044 3030
Size: 2044 986
BstYI r'GATC_y
Cuts at: 0 1455 2123 3008 3030
Size: 1455 668 885 22
Fragments arranged by size:
1455 885 668 22
Bsu36I CC'TnA_GG
Cuts at: 0 256 719 1073 3030
Size: 256 463 354 1957
Fragments arranged by size:
1957 463 354 256
BtgI C'CryG_G
Cuts at: 0 1775 3030
Size: 1775 1255
BtsI GCAGTG_nn'
Cuts at: 0 33 113 164 1112 2298 3030
Size: 33 80 51 948 1186 732
Fragments arranged by size:
1186 948 732 80 51 33
DraI TTT'AAA
Cuts at: 0 1436 3030
Size: 1436 1594
EaeI y'GGCC_r
Cuts at: 0 2180 2972 3030
Size: 2180 792 58
Fragments arranged by size:
2180 792 58
EarI CTCTTCn'nnn_
Cuts at: 0 488 581 1600 2168 2642 2884 3030
Size: 488 93 1019 568 474 242 146
Fragments arranged by size:
1019 568 488 474 242 146 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 569 685 748 1055 1114 1270 1340
Size: 410 159 116 63 307 59 156 70
Cuts at: 1340 1664 1760 1891 1904 2139 2330 2861 2947
Size: 324 96 131 13 235 191 531 86
Cuts at: 2947 3030
Size: 83
Fragments arranged by size:
531 410 324 307 235 191 159 156
131 116 96 86 83 70 63 59
13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 686 2167 3030
Size: 686 1481 863
Fragments arranged by size:
1481 863 686
EcoRI G'AATT_C
Cuts at: 0 2690 3030
Size: 2690 340
EcoRV GAT'ATC
Cuts at: 0 1329 2256 3030
Size: 1329 927 774
Fragments arranged by size:
1329 927 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 592 1423 1477 3030
Size: 592 831 54 1553
Fragments arranged by size:
1553 831 592 54
HaeII r_GCGC'y
Cuts at: 0 2210 3030
Size: 2210 820
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 338 370 1313 1345 1679 1711 2417 2449
Size: 338 32 943 32 334 32 706 32
Cuts at: 2449 3030
Size: 581
Fragments arranged by size:
943 706 581 338 334 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1670 3030
Size: 1670 1360
MfeI C'AATT_G
Cuts at: 0 557 2630 3030
Size: 557 2073 400
Fragments arranged by size:
2073 557 400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1467 1815 2549 3030
Size: 195 1272 348 734 481
Fragments arranged by size:
1272 734 481 348 195
MscI TGG'CCA
Cuts at: 0 2182 2974 3030
Size: 2182 792 56
Fragments arranged by size:
2182 792 56
MslI CAynn'nnrTG
Cuts at: 0 1862 3030
Size: 1862 1168
MspA1I CmG'CkG
Cuts at: 0 460 547 1336 1444 2967 3030
Size: 460 87 789 108 1523 63
Fragments arranged by size:
1523 789 460 108 87 63
NcoI C'CATG_G
Cuts at: 0 1775 3030
Size: 1775 1255
NspI r_CATG'y
Cuts at: 0 786 1731 2023 2355 3030
Size: 786 945 292 332 675
Fragments arranged by size:
945 786 675 332 292
PasI CC'CwG_GG
Cuts at: 0 2129 3030
Size: 2129 901
PciI A'CATG_T
Cuts at: 0 1727 2019 3030
Size: 1727 292 1011
Fragments arranged by size:
1727 1011 292
PfoI T'CCnGG_A
Cuts at: 0 1032 1175 3030
Size: 1032 143 1855
Fragments arranged by size:
1855 1032 143
PmlI CAC'GTG
Cuts at: 0 2323 3030
Size: 2323 707
PsiI TTA'TAA
Cuts at: 0 19 3030
Size: 19 3011
PstI C_TGCA'G
Cuts at: 0 267 403 2958 3030
Size: 267 136 2555 72
Fragments arranged by size:
2555 267 136 72
PvuII CAG'CTG
Cuts at: 0 460 547 1336 1444 2967 3030
Size: 460 87 789 108 1523 63
Fragments arranged by size:
1523 789 460 108 87 63
ScaI AGT'ACT
Cuts at: 0 14 1912 2481 3030
Size: 14 1898 569 549
Fragments arranged by size:
1898 569 549 14
SfcI C'TryA_G
Cuts at: 0 263 399 1983 2954 3030
Size: 263 136 1584 971 76
Fragments arranged by size:
1584 971 263 136 76
SmlI C'TyrA_G
Cuts at: 0 1079 1198 3030
Size: 1079 119 1832
Fragments arranged by size:
1832 1079 119
SphI G_CATG'C
Cuts at: 0 786 2355 3030
Size: 786 1569 675
Fragments arranged by size:
1569 786 675
SspI AAT'ATT
Cuts at: 0 2948 3030
Size: 2948 82
StuI AGG'CCT
Cuts at: 0 499 3030
Size: 499 2531
StyI C'CwwG_G
Cuts at: 0 1046 1698 1775 3030
Size: 1046 652 77 1255
Fragments arranged by size:
1255 1046 652 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2271 2759 2807 3030
Size: 2271 488 48 223
Fragments arranged by size:
2271 488 223 48
TatI w'GTAC_w
Cuts at: 0 12 510 1910 2479 2826 2985 3030
Size: 12 498 1400 569 347 159 45
Fragments arranged by size:
1400 569 498 347 159 45 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 575 607 1082 1114 3030
Size: 455 32 88 32 475 32 1916
Fragments arranged by size:
1916 475 455 88 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2638 2665 3030
Size: 2638 27 365
Fragments arranged by size:
2638 365 27
XbaI T'CTAG_A
Cuts at: 0 858 3030
Size: 858 2172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 365 2044 2719 3030
Size: 336 29 1679 675 311
Fragments arranged by size:
1679 675 336 311 29
XmnI GAAnn'nnTTC
Cuts at: 0 376 572 935 1842 2694 2879 3030
Size: 376 196 363 907 852 185 151
Fragments arranged by size:
907 852 376 363 196 185 151
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BciVI BglI BglII Bme1580I
BmrI BpmI BpuEI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoRI EcoRV FalI HaeII
Hin4I HindIII MfeI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PfoI PmlI PsiI PstI PvuII
ScaI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BssHII BstBI BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I
FseI FspI FspAI HincII HpaI KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI XhoI XmaI ZraI