Restriction map for
pFN21AB0985
(Linear) (Six-base) MAPSORT of: KIBB0985.seq Check: 5421 from: 1 to: 930
KIBB0985.orf
With 182 enzymes: SgfI *
March 22, 2012 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 678 930
Size: 678 252
AfeI AGC'GCT
Cuts at: 0 104 930
Size: 104 826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 930
Size: 526 32 372
Fragments arranged by size:
526 372 32
ApaI G_GGCC'C
Cuts at: 0 226 930
Size: 226 704
ApoI r'AATT_y
Cuts at: 0 88 930
Size: 88 842
AscI GG'CGCG_CC
Cuts at: 0 701 930
Size: 701 229
AvaI C'yCGr_G
Cuts at: 0 128 426 666 744 865 930
Size: 128 298 240 78 121 65
Fragments arranged by size:
298 240 128 121 78 65
BamHI G'GATC_C
Cuts at: 0 288 430 930
Size: 288 142 500
Fragments arranged by size:
500 288 142
BanI G'GyrC_C
Cuts at: 0 229 455 461 670 769 930
Size: 229 226 6 209 99 161
Fragments arranged by size:
229 226 209 161 99 6
BanII G_rGCy'C
Cuts at: 0 226 389 473 874 930
Size: 226 163 84 401 56
Fragments arranged by size:
401 226 163 84 56
BbeI G_GCGC'C
Cuts at: 0 233 459 465 930
Size: 233 226 6 465
Fragments arranged by size:
465 233 226 6
BglI GCCn_nnn'nGGC
Cuts at: 0 31 238 767 930
Size: 31 207 529 163
Fragments arranged by size:
529 207 163 31
Bme1580I G_kGCm'C
Cuts at: 0 226 930
Size: 226 704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 223 271 487 930
Size: 165 58 48 216 443
Fragments arranged by size:
443 216 165 58 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 930
Size: 199 731
BsaHI Gr'CG_yC
Cuts at: 0 230 241 356 376 456 462 930
Size: 230 11 115 20 80 6 468
Fragments arranged by size:
468 230 115 80 20 11 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 930
Size: 526 30 374
Fragments arranged by size:
526 374 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 289 309 930
Size: 286 3 20 621
Fragments arranged by size:
621 286 20 3
BseYI C'CCAG_C
Cuts at: 0 707 930
Size: 707 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 930
Size: 766 164
BsiEI CG_ry'CG
Cuts at: 0 365 930
Size: 365 565
BsmBI CGTCTCn'nnnn_
Cuts at: 0 604 930
Size: 604 326
Bsp1286I G_dGCh'C
Cuts at: 0 226 389 473 874 930
Size: 226 163 84 401 56
Fragments arranged by size:
401 226 163 84 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 678 930
Size: 606 72 252
Fragments arranged by size:
606 252 72
BsrBI CCG'CTC
Cuts at: 0 315 930
Size: 315 615
BsrFI r'CCGG_y
Cuts at: 0 232 458 772 930
Size: 232 226 314 158
Fragments arranged by size:
314 232 226 158
BssHII G'CGCG_C
Cuts at: 0 322 343 492 494 496 701 930
Size: 322 21 149 2 2 205 229
Fragments arranged by size:
322 229 205 149 21 2 2
BstYI r'GATC_y
Cuts at: 0 288 430 930
Size: 288 142 500
Fragments arranged by size:
500 288 142
BtgI C'CryG_G
Cuts at: 0 301 487 809 930
Size: 301 186 322 121
Fragments arranged by size:
322 301 186 121
EaeI y'GGCC_r
Cuts at: 0 234 362 812 930
Size: 234 128 450 118
Fragments arranged by size:
450 234 128 118
EagI C'GGCC_G
Cuts at: 0 362 930
Size: 362 568
EarI CTCTTCn'nnn_
Cuts at: 0 263 829 930
Size: 263 566 101
Fragments arranged by size:
566 263 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 280 822 930
Size: 166 114 542 108
Fragments arranged by size:
542 166 114 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 223 271 487 930
Size: 165 58 48 216 443
Fragments arranged by size:
443 216 165 58 48
EcoO109I rG'GnC_Cy
Cuts at: 0 186 419 930
Size: 186 233 511
Fragments arranged by size:
511 233 186
HaeII r_GCGC'y
Cuts at: 0 106 233 459 465 579 779 930
Size: 106 127 226 6 114 200 151
Fragments arranged by size:
226 200 151 127 114 106 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 930
Size: 163 32 735
Fragments arranged by size:
735 163 32
KasI G'GCGC_C
Cuts at: 0 229 455 461 930
Size: 229 226 6 469
Fragments arranged by size:
469 229 226 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 930
Size: 219 711
MspA1I CmG'CkG
Cuts at: 0 201 303 489 522 811 849 930
Size: 201 102 186 33 289 38 81
Fragments arranged by size:
289 201 186 102 81 38 33
NaeI GCC'GGC
Cuts at: 0 234 460 930
Size: 234 226 470
Fragments arranged by size:
470 234 226
NarI GG'CG_CC
Cuts at: 0 230 456 462 930
Size: 230 226 6 468
Fragments arranged by size:
468 230 226 6
NgoMIV G'CCGG_C
Cuts at: 0 232 458 930
Size: 232 226 472
Fragments arranged by size:
472 232 226
PfoI T'CCnGG_A
Cuts at: 0 65 74 930
Size: 65 9 856
Fragments arranged by size:
856 65 9
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 930
Size: 526 32 372
Fragments arranged by size:
526 372 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 419 930
Size: 186 233 511
Fragments arranged by size:
511 233 186
PshAI GACnn'nnGTC
Cuts at: 0 616 930
Size: 616 314
PspOMI G'GGCC_C
Cuts at: 0 222 930
Size: 222 708
PvuII CAG'CTG
Cuts at: 0 201 522 930
Size: 201 321 408
Fragments arranged by size:
408 321 201
SacII CC_GC'GG
Cuts at: 0 304 490 812 930
Size: 304 186 322 118
Fragments arranged by size:
322 304 186 118
SanDI GG'GwC_CC
Cuts at: 0 186 930
Size: 186 744
SfoI GGC'GCC
Cuts at: 0 231 457 463 930
Size: 231 226 6 467
Fragments arranged by size:
467 231 226 6
SgrAI Cr'CCGG_yG
Cuts at: 0 458 772 930
Size: 458 314 158
Fragments arranged by size:
458 314 158
SmaI CCC'GGG
Cuts at: 0 130 428 668 867 930
Size: 130 298 240 199 63
Fragments arranged by size:
298 240 199 130 63
StyI C'CwwG_G
Cuts at: 0 26 530 888 930
Size: 26 504 358 42
Fragments arranged by size:
504 358 42 26
Tth111I GACn'n_nGTC
Cuts at: 0 190 930
Size: 190 740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 930
Size: 144 786
XmaI C'CCGG_G
Cuts at: 0 128 426 666 865 930
Size: 128 298 240 199 65
Fragments arranged by size:
298 240 199 128 65
XmnI GAAnn'nnTTC
Cuts at: 0 541 930
Size: 541 389
Enzymes that do cut:
AarI AfeI AloI ApaI ApoI AscI AvaI BamHI
BanI BanII BbeI BglI Bme1580I BpmI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BstYI BtgI EaeI EagI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MspA1I
NaeI NarI NgoMIV PfoI PpiI PpuMI PshAI PspOMI
PvuII SacII SanDI SfoI SgrAI SmaI StyI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI XbaI XhoI
ZraI