Restriction map for
pFN21AB0944
(Linear) (Six-base) MAPSORT of: KIBB0944.seq Check: 9391 from: 1 to: 1179
KIBB0944.orf
With 182 enzymes: SgfI *
March 22, 2012 15:31 ..
AflIII A'CryG_T
Cuts at: 0 791 1179
Size: 791 388
AhdI GACnn_n'nnGTC
Cuts at: 0 90 468 1179
Size: 90 378 711
Fragments arranged by size:
711 378 90
AlwNI CAG_nnn'CTG
Cuts at: 0 354 987 1124 1179
Size: 354 633 137 55
Fragments arranged by size:
633 354 137 55
ApaI G_GGCC'C
Cuts at: 0 1161 1179
Size: 1161 18
AvaI C'yCGr_G
Cuts at: 0 182 685 1003 1179
Size: 182 503 318 176
Fragments arranged by size:
503 318 182 176
BamHI G'GATC_C
Cuts at: 0 649 717 1179
Size: 649 68 462
Fragments arranged by size:
649 462 68
BanI G'GyrC_C
Cuts at: 0 139 336 1035 1063 1179
Size: 139 197 699 28 116
Fragments arranged by size:
699 197 139 116 28
BanII G_rGCy'C
Cuts at: 0 675 848 935 954 1105 1161 1179
Size: 675 173 87 19 151 56 18
Fragments arranged by size:
675 173 151 87 56 19 18
BbeI G_GCGC'C
Cuts at: 0 143 340 1067 1179
Size: 143 197 727 112
Fragments arranged by size:
727 197 143 112
BglI GCCn_nnn'nGGC
Cuts at: 0 32 193 1179
Size: 32 161 986
Fragments arranged by size:
986 161 32
BglII A'GATC_T
Cuts at: 0 1146 1179
Size: 1146 33
Bme1580I G_kGCm'C
Cuts at: 0 130 496 866 1040 1161 1179
Size: 130 366 370 174 121 18
Fragments arranged by size:
370 366 174 130 121 18
BmgBI CAC'GTC
Cuts at: 0 92 1179
Size: 92 1087
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 1179
Size: 90 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1035 1171 1179
Size: 911 124 136 8
Fragments arranged by size:
911 136 124 8
Bpu10I CC'TnA_GC
Cuts at: 0 1104 1179
Size: 1104 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 624 919 1179
Size: 424 200 295 260
Fragments arranged by size:
424 295 260 200
BsaHI Gr'CG_yC
Cuts at: 0 140 208 337 640 805 1064 1179
Size: 140 68 129 303 165 259 115
Fragments arranged by size:
303 259 165 140 129 115 68
BsaWI w'CCGG_w
Cuts at: 0 226 1179
Size: 226 953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1008 1038 1179
Size: 70 30 908 30 141
Fragments arranged by size:
908 141 70 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 5 19 93 96 442 1179
Size: 5 14 74 3 346 737
Fragments arranged by size:
737 346 74 14 5 3
BseYI C'CCAG_C
Cuts at: 0 62 68 414 879 945 1179
Size: 62 6 346 465 66 234
Fragments arranged by size:
465 346 234 66 62 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1046 1179
Size: 286 760 133
Fragments arranged by size:
760 286 133
BsiEI CG_ry'CG
Cuts at: 0 344 410 501 1179
Size: 344 66 91 678
Fragments arranged by size:
678 344 91 66
BsiHKAI G_wGCw'C
Cuts at: 0 954 1105 1179
Size: 954 151 74
Fragments arranged by size:
954 151 74
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 800 1179
Size: 361 439 379
Fragments arranged by size:
439 379 361
Bsp1286I G_dGCh'C
Cuts at: 0 130 496 675 848 866 935 954 1040
Size: 130 366 179 173 18 69 19 86
Cuts at: 1040 1105 1161 1179
Size: 65 56 18
Fragments arranged by size:
366 179 173 130 86 69 65 56
19 18 18
BsrBI CCG'CTC
Cuts at: 0 264 1179
Size: 264 915
BsrFI r'CCGG_y
Cuts at: 0 73 339 343 443 1179
Size: 73 266 4 100 736
Fragments arranged by size:
736 266 100 73 4
BssHII G'CGCG_C
Cuts at: 0 220 1179
Size: 220 959
BssSI C'ACGA_G
Cuts at: 0 304 1179
Size: 304 875
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 1179
Size: 309 870
BstXI CCAn_nnnn'nTGG
Cuts at: 0 866 1179
Size: 866 313
BstYI r'GATC_y
Cuts at: 0 47 649 717 1146 1179
Size: 47 602 68 429 33
Fragments arranged by size:
602 429 68 47 33
BtgI C'CryG_G
Cuts at: 0 144 501 1179
Size: 144 357 678
Fragments arranged by size:
678 357 144
BtsI GCAGTG_nn'
Cuts at: 0 445 908 1179
Size: 445 463 271
Fragments arranged by size:
463 445 271
DrdI GACnn_nn'nnGTC
Cuts at: 0 834 1179
Size: 834 345
EaeI y'GGCC_r
Cuts at: 0 33 75 341 345 380 396 407 896
Size: 33 42 266 4 35 16 11 489
Cuts at: 896 1179
Size: 283
Fragments arranged by size:
489 283 266 42 35 33 16 11
4
EagI C'GGCC_G
Cuts at: 0 341 407 1179
Size: 341 66 772
Fragments arranged by size:
772 341 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 254 544 612 648 1179
Size: 254 290 68 36 531
Fragments arranged by size:
531 290 254 68 36
EcoICRI GAG'CTC
Cuts at: 0 952 1103 1179
Size: 952 151 76
Fragments arranged by size:
952 151 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1035 1171 1179
Size: 911 124 136 8
Fragments arranged by size:
911 136 124 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 634 1179
Size: 634 545
EcoO109I rG'GnC_Cy
Cuts at: 0 470 1157 1179
Size: 470 687 22
Fragments arranged by size:
687 470 22
FseI GG_CCGG'CC
Cuts at: 0 77 347 1179
Size: 77 270 832
Fragments arranged by size:
832 270 77
FspI TGC'GCA
Cuts at: 0 277 283 1179
Size: 277 6 896
Fragments arranged by size:
896 277 6
FspAI rTGC'GCAy
Cuts at: 0 283 1179
Size: 283 896
HaeII r_GCGC'y
Cuts at: 0 143 257 340 693 1067 1179
Size: 143 114 83 353 374 112
Fragments arranged by size:
374 353 143 114 112 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 454 486 1179
Size: 76 32 346 32 693
Fragments arranged by size:
693 346 76 32 32
KasI G'GCGC_C
Cuts at: 0 139 336 1063 1179
Size: 139 197 727 116
Fragments arranged by size:
727 197 139 116
MluI A'CGCG_T
Cuts at: 0 791 1179
Size: 791 388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1179
Size: 812 367
MscI TGG'CCA
Cuts at: 0 382 1179
Size: 382 797
MspA1I CmG'CkG
Cuts at: 0 146 235 246 489 1179
Size: 146 89 11 243 690
Fragments arranged by size:
690 243 146 89 11
NaeI GCC'GGC
Cuts at: 0 75 341 345 445 1179
Size: 75 266 4 100 734
Fragments arranged by size:
734 266 100 75 4
NarI GG'CG_CC
Cuts at: 0 140 337 1064 1179
Size: 140 197 727 115
Fragments arranged by size:
727 197 140 115
NgoMIV G'CCGG_C
Cuts at: 0 73 339 343 443 1179
Size: 73 266 4 100 736
Fragments arranged by size:
736 266 100 73 4
NspI r_CATG'y
Cuts at: 0 282 1179
Size: 282 897
PasI CC'CwG_GG
Cuts at: 0 57 722 1179
Size: 57 665 457
Fragments arranged by size:
665 457 57
PpuMI rG'GwC_Cy
Cuts at: 0 470 1179
Size: 470 709
PspOMI G'GGCC_C
Cuts at: 0 1157 1179
Size: 1157 22
PstI C_TGCA'G
Cuts at: 0 178 440 992 1179
Size: 178 262 552 187
Fragments arranged by size:
552 262 187 178
SacI G_AGCT'C
Cuts at: 0 954 1105 1179
Size: 954 151 74
Fragments arranged by size:
954 151 74
SacII CC_GC'GG
Cuts at: 0 147 1179
Size: 147 1032
SanDI GG'GwC_CC
Cuts at: 0 470 1179
Size: 470 709
SfcI C'TryA_G
Cuts at: 0 174 436 988 1179
Size: 174 262 552 191
Fragments arranged by size:
552 262 191 174
SfoI GGC'GCC
Cuts at: 0 141 338 1065 1179
Size: 141 197 727 114
Fragments arranged by size:
727 197 141 114
SmaI CCC'GGG
Cuts at: 0 1005 1179
Size: 1005 174
SphI G_CATG'C
Cuts at: 0 282 1179
Size: 282 897
StuI AGG'CCT
Cuts at: 0 1109 1179
Size: 1109 70
StyI C'CwwG_G
Cuts at: 0 332 475 774 813 1179
Size: 332 143 299 39 366
Fragments arranged by size:
366 332 299 143 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 986 1179
Size: 986 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 1008 1040 1179
Size: 70 32 906 32 139
Fragments arranged by size:
906 139 70 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 831 1179
Size: 831 348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 863 1179
Size: 863 316
XmaI C'CCGG_G
Cuts at: 0 1003 1179
Size: 1003 176
Enzymes that do cut:
AflIII AhdI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI
BstXI BstYI BtgI BtsI DrdI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII
Hin4I KasI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NspI PasI PpuMI PspOMI PstI SacI SacII
SanDI SfcI SfoI SmaI SphI StuI StyI TaqII
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmtI BplI BpuEI BsaAI BsaBI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrGI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EarI
EcoRI EcoRV FalI HincII HindIII HpaI KpnI MfeI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI