Restriction map for
pFN21AB0482
(Linear) (Six-base) MAPSORT of: KIBB0482.seq Check: 892 from: 1 to: 3072
KIBB0482.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AatII G_ACGT'C
Cuts at: 0 1381 3072
Size: 1381 1691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 730 974 2372 2389 3072
Size: 466 264 244 1398 17 683
Fragments arranged by size:
1398 683 466 264 244 17
AflII C'TTAA_G
Cuts at: 0 217 2890 3072
Size: 217 2673 182
Fragments arranged by size:
2673 217 182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 164 560 887 3072
Size: 164 396 327 2185
Fragments arranged by size:
2185 396 327 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 3072
Size: 1186 32 1854
Fragments arranged by size:
1854 1186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 409 2129 3072
Size: 409 1720 943
Fragments arranged by size:
1720 943 409
ApaI G_GGCC'C
Cuts at: 0 624 3072
Size: 624 2448
ApoI r'AATT_y
Cuts at: 0 3 592 754 971 1318 1631 1720 1972
Size: 3 589 162 217 347 313 89 252
Cuts at: 1972 2471 2774 2826 2926 3072
Size: 499 303 52 100 146
Fragments arranged by size:
589 499 347 313 303 252 217 162
146 100 89 52 3
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1467 1500 3072
Size: 1467 33 1572
Fragments arranged by size:
1572 1467 33
BanI G'GyrC_C
Cuts at: 0 871 3072
Size: 871 2201
BanII G_rGCy'C
Cuts at: 0 30 490 562 624 1277 3072
Size: 30 460 72 62 653 1795
Fragments arranged by size:
1795 653 460 72 62 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 911 1012 1365 1892 3072
Size: 591 320 101 353 527 1180
Fragments arranged by size:
1180 591 527 353 320 101
BbvCI CC'TCA_GC
Cuts at: 0 884 2122 3072
Size: 884 1238 950
Fragments arranged by size:
1238 950 884
BclI T'GATC_A
Cuts at: 0 651 938 3072
Size: 651 287 2134
Fragments arranged by size:
2134 651 287
BglI GCCn_nnn'nGGC
Cuts at: 0 1507 3072
Size: 1507 1565
Bme1580I G_kGCm'C
Cuts at: 0 624 876 1824 3072
Size: 624 252 948 1248
Fragments arranged by size:
1248 948 624 252
BmgBI CAC'GTC
Cuts at: 0 2673 3072
Size: 2673 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 1280 3072
Size: 1280 1792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 3072
Size: 1080 1992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1605 2005 2769 3072
Size: 1213 392 400 764 303
Fragments arranged by size:
1213 764 400 392 303
Bpu10I CC'TnA_GC
Cuts at: 0 305 884 1711 2122 2686 3072
Size: 305 579 827 411 564 386
Fragments arranged by size:
827 579 564 411 386 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 2241 3072
Size: 1011 1230 831
Fragments arranged by size:
1230 1011 831
BsaI GGTCTCn'nnnn_
Cuts at: 0 1175 3072
Size: 1175 1897
BsaBI GATnn'nnATC
Cuts at: 0 3029 3072
Size: 3029 43
BsaHI Gr'CG_yC
Cuts at: 0 1378 3072
Size: 1378 1694
BsaWI w'CCGG_w
Cuts at: 0 562 1472 3072
Size: 562 910 1600
Fragments arranged by size:
1600 910 562
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 3072
Size: 1186 30 1856
Fragments arranged by size:
1856 1186 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1267 1408 2725 3072
Size: 1267 141 1317 347
Fragments arranged by size:
1317 1267 347 141
BseYI C'CCAG_C
Cuts at: 0 1933 3072
Size: 1933 1139
BsmI GAATG_Cn'
Cuts at: 0 2552 3072
Size: 2552 520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 616 1385 3072
Size: 616 769 1687
Fragments arranged by size:
1687 769 616
Bsp1286I G_dGCh'C
Cuts at: 0 30 490 562 624 876 1277 1824 3072
Size: 30 460 72 62 252 401 547 1248
Fragments arranged by size:
1248 547 460 401 252 72 62 30
BspEI T'CCGG_A
Cuts at: 0 562 3072
Size: 562 2510
BspHI T'CATG_A
Cuts at: 0 1001 1383 2229 2604 3072
Size: 1001 382 846 375 468
Fragments arranged by size:
1001 846 468 382 375
BstBI TT'CG_AA
Cuts at: 0 1223 3072
Size: 1223 1849
BstEII G'GTnAC_C
Cuts at: 0 1398 3072
Size: 1398 1674
Bsu36I CC'TnA_GG
Cuts at: 0 850 2068 3072
Size: 850 1218 1004
Fragments arranged by size:
1218 1004 850
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1551 3072
Size: 1551 1521
BtsI GCAGTG_nn'
Cuts at: 0 1538 2513 2856 3072
Size: 1538 975 343 216
Fragments arranged by size:
1538 975 343 216
DraI TTT'AAA
Cuts at: 0 314 3057 3072
Size: 314 2743 15
Fragments arranged by size:
2743 314 15
DraIII CAC_nnn'GTG
Cuts at: 0 444 845 3072
Size: 444 401 2227
Fragments arranged by size:
2227 444 401
DrdI GACnn_nn'nnGTC
Cuts at: 0 2400 3072
Size: 2400 672
EaeI y'GGCC_r
Cuts at: 0 698 3072
Size: 698 2374
EarI CTCTTCn'nnn_
Cuts at: 0 485 1904 2606 3072
Size: 485 1419 702 466
Fragments arranged by size:
1419 702 485 466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 730 974 1213 1605 2005 2372 2389
Size: 466 264 244 239 392 400 367 17
Cuts at: 2389 2769 3072
Size: 380 303
Fragments arranged by size:
466 400 392 380 367 303 264 244
239 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 793 3072
Size: 472 321 2279
Fragments arranged by size:
2279 472 321
EcoO109I rG'GnC_Cy
Cuts at: 0 620 1914 2715 3072
Size: 620 1294 801 357
Fragments arranged by size:
1294 801 620 357
EcoRI G'AATT_C
Cuts at: 0 754 1972 3072
Size: 754 1218 1100
Fragments arranged by size:
1218 1100 754
EcoRV GAT'ATC
Cuts at: 0 2028 2035 3072
Size: 2028 7 1037
Fragments arranged by size:
2028 1037 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3019 3072
Size: 3019 53
HaeII r_GCGC'y
Cuts at: 0 914 3072
Size: 914 2158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 1435 1467 1550 1582 1681 1713
Size: 1346 32 57 32 83 32 99 32
Cuts at: 1713 3072
Size: 1359
Fragments arranged by size:
1359 1346 99 83 57 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2981 3072
Size: 2981 91
HindIII A'AGCT_T
Cuts at: 0 1727 1746 2731 3072
Size: 1727 19 985 341
Fragments arranged by size:
1727 985 341 19
HpaI GTT'AAC
Cuts at: 0 2981 3072
Size: 2981 91
MfeI C'AATT_G
Cuts at: 0 1809 3072
Size: 1809 1263
MscI TGG'CCA
Cuts at: 0 700 3072
Size: 700 2372
MslI CAynn'nnrTG
Cuts at: 0 166 700 1136 2320 3072
Size: 166 534 436 1184 752
Fragments arranged by size:
1184 752 534 436 166
MspA1I CmG'CkG
Cuts at: 0 453 3072
Size: 453 2619
NruI TCG'CGA
Cuts at: 0 104 3072
Size: 104 2968
PasI CC'CwG_GG
Cuts at: 0 624 2660 3072
Size: 624 2036 412
Fragments arranged by size:
2036 624 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 554 3072
Size: 554 2518
PfoI T'CCnGG_A
Cuts at: 0 659 1337 1997 3072
Size: 659 678 660 1075
Fragments arranged by size:
1075 678 660 659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 3072
Size: 1186 32 1854
Fragments arranged by size:
1854 1186 32
PpuMI rG'GwC_Cy
Cuts at: 0 2715 3072
Size: 2715 357
PsiI TTA'TAA
Cuts at: 0 58 3072
Size: 58 3014
PspOMI G'GGCC_C
Cuts at: 0 620 3072
Size: 620 2452
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 77 109 3072
Size: 77 32 2963
Fragments arranged by size:
2963 77 32
PstI C_TGCA'G
Cuts at: 0 476 539 1232 1884 3072
Size: 476 63 693 652 1188
Fragments arranged by size:
1188 693 652 476 63
PvuII CAG'CTG
Cuts at: 0 453 3072
Size: 453 2619
SapI GCTCTTCn'nnn_
Cuts at: 0 485 2606 3072
Size: 485 2121 466
Fragments arranged by size:
2121 485 466
SbfI CC_TGCA'GG
Cuts at: 0 476 3072
Size: 476 2596
ScaI AGT'ACT
Cuts at: 0 2919 3072
Size: 2919 153
SfcI C'TryA_G
Cuts at: 0 472 535 1228 1880 2236 3072
Size: 472 63 693 652 356 836
Fragments arranged by size:
836 693 652 472 356 63
SmlI C'TyrA_G
Cuts at: 0 217 990 2220 2890 3072
Size: 217 773 1230 670 182
Fragments arranged by size:
1230 773 670 217 182
SpeI A'CTAG_T
Cuts at: 0 3060 3072
Size: 3060 12
SspI AAT'ATT
Cuts at: 0 2048 3072
Size: 2048 1024
StuI AGG'CCT
Cuts at: 0 1578 2357 3072
Size: 1578 779 715
Fragments arranged by size:
1578 779 715
StyI C'CwwG_G
Cuts at: 0 357 1403 3072
Size: 357 1046 1669
Fragments arranged by size:
1669 1046 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1036 2863 2999 3072
Size: 1036 1827 136 73
Fragments arranged by size:
1827 1036 136 73
TatI w'GTAC_w
Cuts at: 0 1343 1693 2917 3072
Size: 1343 350 1224 155
Fragments arranged by size:
1343 1224 350 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2828 2860 3072
Size: 2828 32 212
Fragments arranged by size:
2828 212 32
Tth111I GACn'n_nGTC
Cuts at: 0 260 3072
Size: 260 2812
XmnI GAAnn'nnTTC
Cuts at: 0 2047 2471 2600 2812 3072
Size: 2047 424 129 212 260
Fragments arranged by size:
2047 424 260 212 129
ZraI GAC'GTC
Cuts at: 0 1379 3072
Size: 1379 1693
Enzymes that do cut:
AatII AcuI AflII AlfI AloI AlwNI ApaI ApoI
BaeI BanI BanII BbsI BbvCI BclI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I
BspEI BspHI BstBI BstEII Bsu36I BtgZI BtsI DraI
DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI
MscI MslI MspA1I NruI PasI PflMI PfoI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuII SapI SbfI
ScaI SfcI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BcgI BciVI BfrBI BglII BlpI BmtI BsaAI
BsgI BsiEI BsiHKAI BsiWI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstYI BstZ17I BtgI
ClaI CspCI EagI EciI EcoICRI FseI FspI FspAI
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PacI PciI PmeI
PmlI PshAI PspXI PvuI RsrII SacI SacII SalI
SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI XbaI XcmI XhoI XmaI