Restriction map for
pFN21AB0472
(Linear) (Six-base) MAPSORT of: KIBB0472.seq Check: 161 from: 1 to: 2979
KIBB0472.orf
With 182 enzymes: SgfI *
July 3, 2012 16:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 577 2073 2695 2783 2979
Size: 577 1496 622 88 196
Fragments arranged by size:
1496 622 577 196 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1009 1102 1189 2132 2979
Size: 163 846 93 87 943 847
Fragments arranged by size:
943 847 846 163 93 87
AgeI A'CCGG_T
Cuts at: 0 2395 2979
Size: 2395 584
AhdI GACnn_n'nnGTC
Cuts at: 0 1518 2979
Size: 1518 1461
AleI CACnn'nnGTG
Cuts at: 0 667 2979
Size: 667 2312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2239 2979
Size: 2239 740
AlwNI CAG_nnn'CTG
Cuts at: 0 89 1572 2065 2979
Size: 89 1483 493 914
Fragments arranged by size:
1483 914 493 89
ApaI G_GGCC'C
Cuts at: 0 2358 2901 2979
Size: 2358 543 78
Fragments arranged by size:
2358 543 78
ApaLI G'TGCA_C
Cuts at: 0 1657 2703 2979
Size: 1657 1046 276
Fragments arranged by size:
1657 1046 276
ApoI r'AATT_y
Cuts at: 0 393 2979
Size: 393 2586
AvaI C'yCGr_G
Cuts at: 0 55 320 1015 1093 1141 2366 2779 2979
Size: 55 265 695 78 48 1225 413 200
Fragments arranged by size:
1225 695 413 265 200 78 55 48
BamHI G'GATC_C
Cuts at: 0 1157 2875 2979
Size: 1157 1718 104
Fragments arranged by size:
1718 1157 104
BanI G'GyrC_C
Cuts at: 0 1406 1664 2979
Size: 1406 258 1315
Fragments arranged by size:
1406 1315 258
BanII G_rGCy'C
Cuts at: 0 56 119 206 2358 2808 2901 2939 2979
Size: 56 63 87 2152 450 93 38 40
Fragments arranged by size:
2152 450 93 87 63 56 40 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1876 2979
Size: 1876 1103
BbvCI CC'TCA_GC
Cuts at: 0 1280 1775 2299 2764 2979
Size: 1280 495 524 465 215
Fragments arranged by size:
1280 524 495 465 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1284 1318 2979
Size: 1284 34 1661
Fragments arranged by size:
1661 1284 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 165 2979
Size: 165 2814
BfrBI ATG'CAT
Cuts at: 0 1861 2979
Size: 1861 1118
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 2171 2674 2896 2979
Size: 1144 1027 503 222 83
Fragments arranged by size:
1144 1027 503 222 83
BglII A'GATC_T
Cuts at: 0 517 2625 2979
Size: 517 2108 354
Fragments arranged by size:
2108 517 354
BlpI GC'TnA_GC
Cuts at: 0 2938 2979
Size: 2938 41
Bme1580I G_kGCm'C
Cuts at: 0 870 1134 1460 1661 1812 2358 2426 2707
Size: 870 264 326 201 151 546 68 281
Cuts at: 2707 2901 2979
Size: 194 78
Fragments arranged by size:
870 546 326 281 264 201 194 151
78 68
BmgBI CAC'GTC
Cuts at: 0 1110 2979
Size: 1110 1869
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 230 317 521 662 2979
Size: 179 51 87 204 141 2317
Fragments arranged by size:
2317 204 179 141 87 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 2979
Size: 120 2859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 103 835 1522 1612 2398 2524 2979
Size: 70 33 732 687 90 786 126 455
Fragments arranged by size:
786 732 687 455 126 90 70 33
Bpu10I CC'TnA_GC
Cuts at: 0 1280 1670 1775 2299 2764 2979
Size: 1280 390 105 524 465 215
Fragments arranged by size:
1280 524 465 390 215 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 1617 2979
Size: 1617 1362
BsaAI yAC'GTr
Cuts at: 0 1821 2979
Size: 1821 1158
BsaHI Gr'CG_yC
Cuts at: 0 1815 2714 2979
Size: 1815 899 265
Fragments arranged by size:
1815 899 265
BsaWI w'CCGG_w
Cuts at: 0 1781 2023 2395 2979
Size: 1781 242 372 584
Fragments arranged by size:
1781 584 372 242
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 849 879 2979
Size: 70 30 749 30 2100
Fragments arranged by size:
2100 749 70 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 480 1297 1519 1522 2302 2343 2653
Size: 91 389 817 222 3 780 41 310
Cuts at: 2653 2925 2979
Size: 272 54
Fragments arranged by size:
817 780 389 310 272 222 91 54
41 3
BseYI C'CCAG_C
Cuts at: 0 483 1349 2092 2128 2385 2403 2886 2900
Size: 483 866 743 36 257 18 483 14
Cuts at: 2900 2979
Size: 79
Fragments arranged by size:
866 743 483 483 257 79 36 18
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 2482 2979
Size: 1339 1143 497
Fragments arranged by size:
1339 1143 497
BsiHKAI G_wGCw'C
Cuts at: 0 56 119 509 1661 2294 2707 2939 2979
Size: 56 63 390 1152 633 413 232 40
Fragments arranged by size:
1152 633 413 390 232 63 56 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 56 1441 2979
Size: 56 1385 1538
Fragments arranged by size:
1538 1385 56
Bsp1286I G_dGCh'C
Cuts at: 0 56 119 206 509 870 1134 1460 1661
Size: 56 63 87 303 361 264 326 201
Cuts at: 1661 1812 2294 2358 2426 2707 2808 2901 2939
Size: 151 482 64 68 281 101 93 38
Cuts at: 2939 2979
Size: 40
Fragments arranged by size:
482 361 326 303 281 264 201 151
101 93 87 68 64 63 56 40
38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 74 141 577 1646 2013 2073 2695
Size: 53 21 67 436 1069 367 60 622
Cuts at: 2695 2783 2979
Size: 88 196
Fragments arranged by size:
1069 622 436 367 196 88 67 60
53 21
BsrDI GCAATG_nn'
Cuts at: 0 1601 2979
Size: 1601 1378
BsrFI r'CCGG_y
Cuts at: 0 1480 2088 2395 2710 2854 2979
Size: 1480 608 307 315 144 125
Fragments arranged by size:
1480 608 315 307 144 125
BssHII G'CGCG_C
Cuts at: 0 2528 2979
Size: 2528 451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2065 2979
Size: 2065 914
BstBI TT'CG_AA
Cuts at: 0 957 2979
Size: 957 2022
BstEII G'GTnAC_C
Cuts at: 0 192 324 2979
Size: 192 132 2655
Fragments arranged by size:
2655 192 132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 169 669 2199 2979
Size: 39 130 500 1530 780
Fragments arranged by size:
1530 780 500 130 39
BstYI r'GATC_y
Cuts at: 0 311 517 1157 1741 2625 2783 2875 2979
Size: 311 206 640 584 884 158 92 104
Fragments arranged by size:
884 640 584 311 206 158 104 92
Bsu36I CC'TnA_GG
Cuts at: 0 1414 2565 2979
Size: 1414 1151 414
Fragments arranged by size:
1414 1151 414
BtgI C'CryG_G
Cuts at: 0 221 636 740 1397 2633 2787 2979
Size: 221 415 104 657 1236 154 192
Fragments arranged by size:
1236 657 415 221 192 154 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 2979
Size: 720 2259
DrdI GACnn_nn'nnGTC
Cuts at: 0 1114 1936 2979
Size: 1114 822 1043
Fragments arranged by size:
1114 1043 822
EaeI y'GGCC_r
Cuts at: 0 302 2210 2618 2979
Size: 302 1908 408 361
Fragments arranged by size:
1908 408 361 302
EarI CTCTTCn'nnn_
Cuts at: 0 236 1398 1439 2320 2979
Size: 236 1162 41 881 659
Fragments arranged by size:
1162 881 659 236 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1534 2979
Size: 1534 1445
EcoICRI GAG'CTC
Cuts at: 0 54 117 2937 2979
Size: 54 63 2820 42
Fragments arranged by size:
2820 63 54 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 103 163 835 1009 1102 1189 1522
Size: 70 33 60 672 174 93 87 333
Cuts at: 1522 1612 2132 2398 2524 2979
Size: 90 520 266 126 455
Fragments arranged by size:
672 520 455 333 266 174 126 93
90 87 70 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1168 1279 2979
Size: 1168 111 1700
Fragments arranged by size:
1700 1168 111
EcoO109I rG'GnC_Cy
Cuts at: 0 86 1137 1419 2002 2354 2355 2380 2652
Size: 86 1051 282 583 352 1 25 272
Cuts at: 2652 2844 2897 2979
Size: 192 53 82
Fragments arranged by size:
1051 583 352 282 272 192 86 82
53 25 1
EcoRI G'AATT_C
Cuts at: 0 393 2979
Size: 393 2586
EcoRV GAT'ATC
Cuts at: 0 261 885 2979
Size: 261 624 2094
Fragments arranged by size:
2094 624 261
FspI TGC'GCA
Cuts at: 0 2438 2979
Size: 2438 541
HaeII r_GCGC'y
Cuts at: 0 762 2979
Size: 762 2217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 1241 1273 1504 1536 2979
Size: 70 32 1139 32 231 32 1443
Fragments arranged by size:
1443 1139 231 70 32 32 32
HincII GTy'rAC
Cuts at: 0 1851 2979
Size: 1851 1128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1769 2210 2979
Size: 755 1014 441 769
Fragments arranged by size:
1014 769 755 441
MscI TGG'CCA
Cuts at: 0 304 2212 2979
Size: 304 1908 767
Fragments arranged by size:
1908 767 304
MslI CAynn'nnrTG
Cuts at: 0 502 667 739 1606 2197 2341 2979
Size: 502 165 72 867 591 144 638
Fragments arranged by size:
867 638 591 502 165 144 72
MspA1I CmG'CkG
Cuts at: 0 13 223 300 552 780 1209 2034 2861
Size: 13 210 77 252 228 429 825 827
Cuts at: 2861 2904 2979
Size: 43 75
Fragments arranged by size:
827 825 429 252 228 210 77 75
43 13
NaeI GCC'GGC
Cuts at: 0 1482 2090 2712 2856 2979
Size: 1482 608 622 144 123
Fragments arranged by size:
1482 622 608 144 123
NcoI C'CATG_G
Cuts at: 0 636 2787 2979
Size: 636 2151 192
Fragments arranged by size:
2151 636 192
NgoMIV G'CCGG_C
Cuts at: 0 1480 2088 2710 2854 2979
Size: 1480 608 622 144 125
Fragments arranged by size:
1480 622 608 144 125
NsiI A_TGCA'T
Cuts at: 0 1863 2979
Size: 1863 1116
NspI r_CATG'y
Cuts at: 0 1332 2979
Size: 1332 1647
PasI CC'CwG_GG
Cuts at: 0 1968 1998 2376 2456 2724 2979
Size: 1968 30 378 80 268 255
Fragments arranged by size:
1968 378 268 255 80 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 1572 2979
Size: 1572 1407
PfoI T'CCnGG_A
Cuts at: 0 812 2979
Size: 812 2167
PmlI CAC'GTG
Cuts at: 0 1821 2979
Size: 1821 1158
PpuMI rG'GwC_Cy
Cuts at: 0 86 1419 2002 2380 2844 2979
Size: 86 1333 583 378 464 135
Fragments arranged by size:
1333 583 464 378 135 86
PshAI GACnn'nnGTC
Cuts at: 0 322 2979
Size: 322 2657
PspOMI G'GGCC_C
Cuts at: 0 2354 2897 2979
Size: 2354 543 82
Fragments arranged by size:
2354 543 82
PspXI vC'TCGA_Gb
Cuts at: 0 1141 2979
Size: 1141 1838
PstI C_TGCA'G
Cuts at: 0 152 2061 2516 2749 2909 2979
Size: 152 1909 455 233 160 70
Fragments arranged by size:
1909 455 233 160 152 70
PvuII CAG'CTG
Cuts at: 0 13 300 1209 2904 2979
Size: 13 287 909 1695 75
Fragments arranged by size:
1695 909 287 75 13
SacI G_AGCT'C
Cuts at: 0 56 119 2939 2979
Size: 56 63 2820 40
Fragments arranged by size:
2820 63 56 40
SacII CC_GC'GG
Cuts at: 0 224 2979
Size: 224 2755
SanDI GG'GwC_CC
Cuts at: 0 2380 2979
Size: 2380 599
SapI GCTCTTCn'nnn_
Cuts at: 0 236 2979
Size: 236 2743
SexAI A'CCwGG_T
Cuts at: 0 902 2979
Size: 902 2077
SfcI C'TryA_G
Cuts at: 0 148 399 2057 2512 2745 2905 2979
Size: 148 251 1658 455 233 160 74
Fragments arranged by size:
1658 455 251 233 160 148 74
SmaI CCC'GGG
Cuts at: 0 322 2979
Size: 322 2657
SmlI C'TyrA_G
Cuts at: 0 55 1015 1141 2979
Size: 55 960 126 1838
Fragments arranged by size:
1838 960 126 55
SphI G_CATG'C
Cuts at: 0 1332 2979
Size: 1332 1647
StuI AGG'CCT
Cuts at: 0 2563 2979
Size: 2563 416
StyI C'CwwG_G
Cuts at: 0 636 1371 1701 2787 2979
Size: 636 735 330 1086 192
Fragments arranged by size:
1086 735 636 330 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 327 343 401 2979
Size: 327 16 58 2578
Fragments arranged by size:
2578 327 58 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 849 881 2979
Size: 849 32 2098
Fragments arranged by size:
2098 849 32
Tth111I GACn'n_nGTC
Cuts at: 0 904 2979
Size: 904 2075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 643 738 2979
Size: 643 95 2241
Fragments arranged by size:
2241 643 95
XhoI C'TCGA_G
Cuts at: 0 55 1015 1141 2979
Size: 55 960 126 1838
Fragments arranged by size:
1838 960 126 55
XmaI C'CCGG_G
Cuts at: 0 320 2979
Size: 320 2659
Enzymes that do cut:
AarI AcuI AgeI AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SexAI SfcI SmaI SmlI SphI StuI StyI TaqII
TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AloI
AscI AseI AsiSI AvrII BaeI BbeI BclI BmtI
BpuEI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BssSI BstZ17I BtsI ClaI CspCI DraI DraIII
EagI FalI FseI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI PacI
PciI PmeI PpiI PsiI PsrI PvuI RsrII SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XmnI ZraI