Restriction map for
pFN21AB0466
(Linear) (Six-base) MAPSORT of: KIBB0466.seq Check: 3927 from: 1 to: 2964
KIBB0466.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 904 2964
Size: 904 2060
AccI GT'mk_AC
Cuts at: 0 872 1251 1712 2964
Size: 872 379 461 1252
Fragments arranged by size:
1252 872 461 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 988 1588 1789 2222 2657 2964
Size: 485 503 600 201 433 435 307
Fragments arranged by size:
600 503 485 435 433 307 201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2724 2756 2964
Size: 2724 32 208
Fragments arranged by size:
2724 208 32
AlwNI CAG_nnn'CTG
Cuts at: 0 493 711 732 1417 1771 2964
Size: 493 218 21 685 354 1193
Fragments arranged by size:
1193 685 493 354 218 21
ApaI G_GGCC'C
Cuts at: 0 242 311 2964
Size: 242 69 2653
Fragments arranged by size:
2653 242 69
ApaLI G'TGCA_C
Cuts at: 0 721 2609 2964
Size: 721 1888 355
Fragments arranged by size:
1888 721 355
ApoI r'AATT_y
Cuts at: 0 82 1390 2964
Size: 82 1308 1574
Fragments arranged by size:
1574 1308 82
AvaI C'yCGr_G
Cuts at: 0 25 173 268 304 447 778 823 946
Size: 25 148 95 36 143 331 45 123
Cuts at: 946 2964
Size: 2018
Fragments arranged by size:
2018 331 148 143 123 95 45 36
25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2050 2083 2964
Size: 2050 33 881
Fragments arranged by size:
2050 881 33
BamHI G'GATC_C
Cuts at: 0 2549 2964
Size: 2549 415
BanI G'GyrC_C
Cuts at: 0 199 510 609 817 2964
Size: 199 311 99 208 2147
Fragments arranged by size:
2147 311 208 199 99
BanII G_rGCy'C
Cuts at: 0 242 304 311 322 427 1034 2665 2964
Size: 242 62 7 11 105 607 1631 299
Fragments arranged by size:
1631 607 299 242 105 62 11 7
BbeI G_GCGC'C
Cuts at: 0 203 514 821 2964
Size: 203 311 307 2143
Fragments arranged by size:
2143 311 307 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 1197 2964
Size: 1197 1767
BbvCI CC'TCA_GC
Cuts at: 0 967 2574 2964
Size: 967 1607 390
Fragments arranged by size:
1607 967 390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 259 293 2964
Size: 259 34 2671
Fragments arranged by size:
2671 259 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2078 2964
Size: 2078 886
BclI T'GATC_A
Cuts at: 0 1380 2964
Size: 1380 1584
BglI GCCn_nnn'nGGC
Cuts at: 0 254 508 2964
Size: 254 254 2456
Fragments arranged by size:
2456 254 254
BglII A'GATC_T
Cuts at: 0 1311 2517 2964
Size: 1311 1206 447
Fragments arranged by size:
1311 1206 447
Bme1580I G_kGCm'C
Cuts at: 0 242 311 725 2571 2613 2964
Size: 242 69 414 1846 42 351
Fragments arranged by size:
1846 414 351 242 69 42
BmgBI CAC'GTC
Cuts at: 0 1837 2964
Size: 1837 1127
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 421 2964
Size: 45 376 2543
Fragments arranged by size:
2543 376 45
BmtI G_CTAG'C
Cuts at: 0 2686 2964
Size: 2686 278
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 357 2964
Size: 357 2607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 679 850 1423 1768 2530 2964
Size: 34 645 171 573 345 762 434
Fragments arranged by size:
762 645 573 434 345 171 34
Bpu10I CC'TnA_GC
Cuts at: 0 967 2486 2574 2964
Size: 967 1519 88 390
Fragments arranged by size:
1519 967 390 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 570 928 2964
Size: 570 358 2036
Fragments arranged by size:
2036 570 358
BsaHI Gr'CG_yC
Cuts at: 0 200 511 818 2964
Size: 200 311 307 2146
Fragments arranged by size:
2146 311 307 200
BsaWI w'CCGG_w
Cuts at: 0 143 2964
Size: 143 2821
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2724 2754 2964
Size: 2724 30 210
Fragments arranged by size:
2724 210 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 177 335 586 964 2438 2664 2667
Size: 31 146 158 251 378 1474 226 3
Cuts at: 2667 2947 2964
Size: 280 17
Fragments arranged by size:
1474 378 280 251 226 158 146 31
17 3
BseYI C'CCAG_C
Cuts at: 0 683 2964
Size: 683 2281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 2150 2964
Size: 699 1451 814
Fragments arranged by size:
1451 814 699
BsiEI CG_ry'CG
Cuts at: 0 508 671 2964
Size: 508 163 2293
Fragments arranged by size:
2293 508 163
BsiHKAI G_wGCw'C
Cuts at: 0 322 355 725 739 926 1034 2613 2665
Size: 322 33 370 14 187 108 1579 52
Cuts at: 2665 2964
Size: 299
Fragments arranged by size:
1579 370 322 299 187 108 52 33
14
BsmI GAATG_Cn'
Cuts at: 0 1376 2964
Size: 1376 1588
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 2964
Size: 13 2951
Bsp1286I G_dGCh'C
Cuts at: 0 242 304 311 322 355 427 725 739
Size: 242 62 7 11 33 72 298 14
Cuts at: 739 926 1034 2571 2613 2665 2964
Size: 187 108 1537 42 52 299
Fragments arranged by size:
1537 299 298 242 187 108 72 62
52 42 33 14 11 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 719 904 2964
Size: 142 577 185 2060
Fragments arranged by size:
2060 577 185 142
BsrBI CCG'CTC
Cuts at: 0 167 2964
Size: 167 2797
BsrFI r'CCGG_y
Cuts at: 0 208 507 768 2964
Size: 208 299 261 2196
Fragments arranged by size:
2196 299 261 208
BssHII G'CGCG_C
Cuts at: 0 714 2964
Size: 714 2250
BstAPI GCAn_nnn'nTGC
Cuts at: 0 493 1417 1536 1771 2964
Size: 493 924 119 235 1193
Fragments arranged by size:
1193 924 493 235 119
BstBI TT'CG_AA
Cuts at: 0 2049 2964
Size: 2049 915
BstXI CCAn_nnnn'nTGG
Cuts at: 0 657 2964
Size: 657 2307
BstYI r'GATC_y
Cuts at: 0 908 1311 2517 2549 2964
Size: 908 403 1206 32 415
Fragments arranged by size:
1206 908 415 403 32
BstZ17I GTA'TAC
Cuts at: 0 1713 2964
Size: 1713 1251
BtgI C'CryG_G
Cuts at: 0 234 376 882 1211 2669 2964
Size: 234 142 506 329 1458 295
Fragments arranged by size:
1458 506 329 295 234 142
BtsI GCAGTG_nn'
Cuts at: 0 275 2964
Size: 275 2689
DraI TTT'AAA
Cuts at: 0 2058 2964
Size: 2058 906
DraIII CAC_nnn'GTG
Cuts at: 0 854 2964
Size: 854 2110
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 2964
Size: 27 2937
EaeI y'GGCC_r
Cuts at: 0 505 668 2964
Size: 505 163 2296
Fragments arranged by size:
2296 505 163
EagI C'GGCC_G
Cuts at: 0 505 668 2964
Size: 505 163 2296
Fragments arranged by size:
2296 505 163
EarI CTCTTCn'nnn_
Cuts at: 0 668 931 1517 1637 1751 1784 2186 2198
Size: 668 263 586 120 114 33 402 12
Cuts at: 2198 2214 2360 2423 2539 2924 2964
Size: 16 146 63 116 385 40
Fragments arranged by size:
668 586 402 385 263 146 120 116
114 63 40 33 16 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 227 330 346 421 2964
Size: 227 103 16 75 2543
Fragments arranged by size:
2543 227 103 75 16
EcoICRI GAG'CTC
Cuts at: 0 320 1032 2663 2964
Size: 320 712 1631 301
Fragments arranged by size:
1631 712 320 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 485 679 850 988 1423 1588 1768
Size: 34 451 194 171 138 435 165 180
Cuts at: 1768 1789 2222 2530 2657 2964
Size: 21 433 308 127 307
Fragments arranged by size:
451 435 433 308 307 194 180 171
165 138 127 34 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 916 2964
Size: 916 2048
EcoO109I rG'GnC_Cy
Cuts at: 0 308 372 708 1231 2964
Size: 308 64 336 523 1733
Fragments arranged by size:
1733 523 336 308 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1018 2101 2964
Size: 1018 1083 863
Fragments arranged by size:
1083 1018 863
HaeII r_GCGC'y
Cuts at: 0 203 514 554 622 821 2964
Size: 203 311 40 68 199 2143
Fragments arranged by size:
2143 311 203 199 68 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 927 959 1184 1216 2964
Size: 646 32 249 32 225 32 1748
Fragments arranged by size:
1748 646 249 225 32 32 32
KasI G'GCGC_C
Cuts at: 0 199 510 817 2964
Size: 199 311 307 2147
Fragments arranged by size:
2147 311 307 199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 248 734 1070 1938 2449 2964
Size: 11 237 486 336 868 511 515
Fragments arranged by size:
868 515 511 486 336 237 11
MspA1I CmG'CkG
Cuts at: 0 236 378 493 683 732 1008 1278 1414
Size: 236 142 115 190 49 276 270 136
Cuts at: 1414 1503 1824 2601 2964
Size: 89 321 777 363
Fragments arranged by size:
777 363 321 276 270 236 190 142
136 115 89 49
NaeI GCC'GGC
Cuts at: 0 210 509 2964
Size: 210 299 2455
Fragments arranged by size:
2455 299 210
NarI GG'CG_CC
Cuts at: 0 200 511 818 2964
Size: 200 311 307 2146
Fragments arranged by size:
2146 311 307 200
NcoI C'CATG_G
Cuts at: 0 1211 2669 2964
Size: 1211 1458 295
Fragments arranged by size:
1458 1211 295
NgoMIV G'CCGG_C
Cuts at: 0 208 507 2964
Size: 208 299 2457
Fragments arranged by size:
2457 299 208
NheI G'CTAG_C
Cuts at: 0 2682 2964
Size: 2682 282
NspI r_CATG'y
Cuts at: 0 798 1513 2964
Size: 798 715 1451
Fragments arranged by size:
1451 798 715
PflMI CCAn_nnn'nTGG
Cuts at: 0 888 2964
Size: 888 2076
PfoI T'CCnGG_A
Cuts at: 0 704 1025 2964
Size: 704 321 1939
Fragments arranged by size:
1939 704 321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2724 2756 2964
Size: 2724 32 208
Fragments arranged by size:
2724 208 32
PpuMI rG'GwC_Cy
Cuts at: 0 372 708 1231 2964
Size: 372 336 523 1733
Fragments arranged by size:
1733 523 372 336
PspOMI G'GGCC_C
Cuts at: 0 238 307 2964
Size: 238 69 2657
Fragments arranged by size:
2657 238 69
PspXI vC'TCGA_Gb
Cuts at: 0 25 2964
Size: 25 2939
PstI C_TGCA'G
Cuts at: 0 498 1799 2964
Size: 498 1301 1165
Fragments arranged by size:
1301 1165 498
PvuII CAG'CTG
Cuts at: 0 683 732 1824 2601 2964
Size: 683 49 1092 777 363
Fragments arranged by size:
1092 777 683 363 49
SacI G_AGCT'C
Cuts at: 0 322 1034 2665 2964
Size: 322 712 1631 299
Fragments arranged by size:
1631 712 322 299
SacII CC_GC'GG
Cuts at: 0 237 379 2964
Size: 237 142 2585
Fragments arranged by size:
2585 237 142
SanDI GG'GwC_CC
Cuts at: 0 372 2964
Size: 372 2592
SapI GCTCTTCn'nnn_
Cuts at: 0 931 1784 2964
Size: 931 853 1180
Fragments arranged by size:
1180 931 853
ScaI AGT'ACT
Cuts at: 0 1327 2964
Size: 1327 1637
SfcI C'TryA_G
Cuts at: 0 494 1104 1150 1795 2964
Size: 494 610 46 645 1169
Fragments arranged by size:
1169 645 610 494 46
SfoI GGC'GCC
Cuts at: 0 201 512 819 2964
Size: 201 311 307 2145
Fragments arranged by size:
2145 311 307 201
SmaI CCC'GGG
Cuts at: 0 175 306 449 2964
Size: 175 131 143 2515
Fragments arranged by size:
2515 175 143 131
SmlI C'TyrA_G
Cuts at: 0 25 2964
Size: 25 2939
StyI C'CwwG_G
Cuts at: 0 1211 2669 2964
Size: 1211 1458 295
Fragments arranged by size:
1458 1211 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 847 952 2682 2964
Size: 27 820 105 1730 282
Fragments arranged by size:
1730 820 282 105 27
TatI w'GTAC_w
Cuts at: 0 1296 1325 1617 1959 2964
Size: 1296 29 292 342 1005
Fragments arranged by size:
1296 1005 342 292 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2861 2893 2964
Size: 2861 32 71
Fragments arranged by size:
2861 71 32
XhoI C'TCGA_G
Cuts at: 0 25 2964
Size: 25 2939
XmaI C'CCGG_G
Cuts at: 0 173 304 447 2964
Size: 173 131 143 2517
Fragments arranged by size:
2517 173 143 131
XmnI GAAnn'nnTTC
Cuts at: 0 1001 2964
Size: 1001 1963
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI ScaI SfcI
SfoI SmaI SmlI StyI TaqII TatI TstI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BfrBI BlpI
BpuEI BsaAI BsaBI BsiWI BspEI BspHI BsrDI BsrGI
BssSI BstEII Bsu36I BtgZI ClaI CspCI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI MslI NdeI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI ZraI