Restriction map for
pFN21AB0450
(Linear) (Six-base) MAPSORT of: KIBB0450.seq Check: 5294 from: 1 to: 2589
KIBB0450.orf
With 182 enzymes: SgfI *
March 22, 2012 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 173 2589
Size: 173 2416
AccI GT'mk_AC
Cuts at: 0 1771 2589
Size: 1771 818
AclI AA'CG_TT
Cuts at: 0 1293 2589
Size: 1293 1296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 1660 2589
Size: 1602 58 929
Fragments arranged by size:
1602 929 58
AflIII A'CryG_T
Cuts at: 0 1096 1237 2589
Size: 1096 141 1352
Fragments arranged by size:
1352 1096 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 2589
Size: 571 2018
AlwNI CAG_nnn'CTG
Cuts at: 0 257 2589
Size: 257 2332
ApoI r'AATT_y
Cuts at: 0 745 1175 1186 2589
Size: 745 430 11 1403
Fragments arranged by size:
1403 745 430 11
AseI AT'TA_AT
Cuts at: 0 496 1015 2589
Size: 496 519 1574
Fragments arranged by size:
1574 519 496
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 375 408 2589
Size: 375 33 2181
Fragments arranged by size:
2181 375 33
BanII G_rGCy'C
Cuts at: 0 16 2062 2589
Size: 16 2046 527
Fragments arranged by size:
2046 527 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 2255 2589
Size: 292 1963 334
Fragments arranged by size:
1963 334 292
BbvCI CC'TCA_GC
Cuts at: 0 1919 2589
Size: 1919 670
BciVI GTATCCnnnnn_n'
Cuts at: 0 2126 2589
Size: 2126 463
BfrBI ATG'CAT
Cuts at: 0 2086 2589
Size: 2086 503
BglI GCCn_nnn'nGGC
Cuts at: 0 962 2394 2589
Size: 962 1432 195
Fragments arranged by size:
1432 962 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 665 2589
Size: 665 1924
Bpu10I CC'TnA_GC
Cuts at: 0 75 1919 2589
Size: 75 1844 670
Fragments arranged by size:
1844 670 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1137 2539 2589
Size: 1137 1402 50
Fragments arranged by size:
1402 1137 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 1067 1412 2589
Size: 1067 345 1177
Fragments arranged by size:
1177 1067 345
BsaAI yAC'GTr
Cuts at: 0 1238 2268 2589
Size: 1238 1030 321
Fragments arranged by size:
1238 1030 321
BsaWI w'CCGG_w
Cuts at: 0 166 2589
Size: 166 2423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 579 609 2180 2210 2589
Size: 579 30 1571 30 379
Fragments arranged by size:
1571 579 379 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 1780 2422 2589
Size: 24 1756 642 167
Fragments arranged by size:
1756 642 167 24
BseYI C'CCAG_C
Cuts at: 0 299 2589
Size: 299 2290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1683 2589
Size: 71 1612 906
Fragments arranged by size:
1612 906 71
BsiHKAI G_wGCw'C
Cuts at: 0 16 2062 2589
Size: 16 2046 527
Fragments arranged by size:
2046 527 16
BsmI GAATG_Cn'
Cuts at: 0 472 2086 2589
Size: 472 1614 503
Fragments arranged by size:
1614 503 472
Bsp1286I G_dGCh'C
Cuts at: 0 16 2062 2589
Size: 16 2046 527
Fragments arranged by size:
2046 527 16
BspEI T'CCGG_A
Cuts at: 0 166 2589
Size: 166 2423
BspHI T'CATG_A
Cuts at: 0 891 2456 2589
Size: 891 1565 133
Fragments arranged by size:
1565 891 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 1812 2573 2589
Size: 173 1639 761 16
Fragments arranged by size:
1639 761 173 16
BsrBI CCG'CTC
Cuts at: 0 849 2589
Size: 849 1740
BsrFI r'CCGG_y
Cuts at: 0 609 2393 2589
Size: 609 1784 196
Fragments arranged by size:
1784 609 196
BssSI C'ACGA_G
Cuts at: 0 844 2589
Size: 844 1745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2325 2589
Size: 2325 264
BstYI r'GATC_y
Cuts at: 0 169 2589
Size: 169 2420
BtgI C'CryG_G
Cuts at: 0 294 509 1077 1422 1581 2589
Size: 294 215 568 345 159 1008
Fragments arranged by size:
1008 568 345 294 215 159
BtsI GCAGTG_nn'
Cuts at: 0 83 354 877 1814 2589
Size: 83 271 523 937 775
Fragments arranged by size:
937 775 523 271 83
EaeI y'GGCC_r
Cuts at: 0 374 650 2589
Size: 374 276 1939
Fragments arranged by size:
1939 374 276
EarI CTCTTCn'nnn_
Cuts at: 0 1772 2067 2589
Size: 1772 295 522
Fragments arranged by size:
1772 522 295
EcoICRI GAG'CTC
Cuts at: 0 14 2060 2589
Size: 14 2046 529
Fragments arranged by size:
2046 529 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 1660 2589
Size: 1602 58 929
Fragments arranged by size:
1602 929 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 603 2589
Size: 603 1986
EcoO109I rG'GnC_Cy
Cuts at: 0 124 1577 2589
Size: 124 1453 1012
Fragments arranged by size:
1453 1012 124
EcoRI G'AATT_C
Cuts at: 0 1175 2589
Size: 1175 1414
EcoRV GAT'ATC
Cuts at: 0 1329 1833 2589
Size: 1329 504 756
Fragments arranged by size:
1329 756 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 1388 2589
Size: 1287 101 1201
Fragments arranged by size:
1287 1201 101
FspI TGC'GCA
Cuts at: 0 1606 2589
Size: 1606 983
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 963 966 995 998 1775 1807
Size: 53 32 878 3 29 3 777 32
Cuts at: 1807 1817 1849 1974 2006 2419 2451 2589
Size: 10 32 125 32 413 32 138
Fragments arranged by size:
878 777 413 138 125 53 32 32
32 32 32 29 10 3 3
HincII GTy'rAC
Cuts at: 0 772 1056 1443 2193 2589
Size: 772 284 387 750 396
Fragments arranged by size:
772 750 396 387 284
HpaI GTT'AAC
Cuts at: 0 2193 2589
Size: 2193 396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1050 1715 2317 2589
Size: 39 1011 665 602 272
Fragments arranged by size:
1011 665 602 272 39
MscI TGG'CCA
Cuts at: 0 376 652 2589
Size: 376 276 1937
Fragments arranged by size:
1937 376 276
MslI CAynn'nnrTG
Cuts at: 0 950 1049 2461 2573 2589
Size: 950 99 1412 112 16
Fragments arranged by size:
1412 950 112 99 16
MspA1I CmG'CkG
Cuts at: 0 2321 2589
Size: 2321 268
NcoI C'CATG_G
Cuts at: 0 1077 1581 2589
Size: 1077 504 1008
Fragments arranged by size:
1077 1008 504
NdeI CA'TA_TG
Cuts at: 0 2574 2589
Size: 2574 15
NsiI A_TGCA'T
Cuts at: 0 2088 2589
Size: 2088 501
NspI r_CATG'y
Cuts at: 0 1100 1360 1452 2589
Size: 1100 260 92 1137
Fragments arranged by size:
1137 1100 260 92
PciI A'CATG_T
Cuts at: 0 1096 2589
Size: 1096 1493
PflMI CCAn_nnn'nTGG
Cuts at: 0 1083 1338 2589
Size: 1083 255 1251
Fragments arranged by size:
1251 1083 255
PmlI CAC'GTG
Cuts at: 0 1238 2589
Size: 1238 1351
PpuMI rG'GwC_Cy
Cuts at: 0 124 1577 2589
Size: 124 1453 1012
Fragments arranged by size:
1453 1012 124
PsiI TTA'TAA
Cuts at: 0 1385 2589
Size: 1385 1204
PstI C_TGCA'G
Cuts at: 0 477 1809 2589
Size: 477 1332 780
Fragments arranged by size:
1332 780 477
PvuII CAG'CTG
Cuts at: 0 2321 2589
Size: 2321 268
SacI G_AGCT'C
Cuts at: 0 16 2062 2589
Size: 16 2046 527
Fragments arranged by size:
2046 527 16
SanDI GG'GwC_CC
Cuts at: 0 1577 2589
Size: 1577 1012
SapI GCTCTTCn'nnn_
Cuts at: 0 2067 2589
Size: 2067 522
SbfI CC_TGCA'GG
Cuts at: 0 477 2589
Size: 477 2112
SfcI C'TryA_G
Cuts at: 0 131 473 1772 1805 2304 2589
Size: 131 342 1299 33 499 285
Fragments arranged by size:
1299 499 342 285 131 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 962 2589
Size: 962 1627
SmlI C'TyrA_G
Cuts at: 0 1116 2554 2589
Size: 1116 1438 35
Fragments arranged by size:
1438 1116 35
SphI G_CATG'C
Cuts at: 0 1452 2589
Size: 1452 1137
SspI AAT'ATT
Cuts at: 0 564 857 1874 2589
Size: 564 293 1017 715
Fragments arranged by size:
1017 715 564 293
StuI AGG'CCT
Cuts at: 0 900 2589
Size: 900 1689
StyI C'CwwG_G
Cuts at: 0 1077 1581 2589
Size: 1077 504 1008
Fragments arranged by size:
1077 1008 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 894 2589
Size: 894 1695
TatI w'GTAC_w
Cuts at: 0 307 1487 2589
Size: 307 1180 1102
Fragments arranged by size:
1180 1102 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 579 611 2180 2212 2589
Size: 579 32 1569 32 377
Fragments arranged by size:
1569 579 377 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 2589
Size: 402 2187
XmnI GAAnn'nnTTC
Cuts at: 0 2143 2589
Size: 2143 446
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AlfI AlwNI ApoI
AseI BaeI BanII BbsI BbvCI BciVI BfrBI BglI
BmrI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssSI BstXI BstYI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PmlI PpuMI
PsiI PstI PvuII SacI SanDI SapI SbfI SfcI
SfiI SmlI SphI SspI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanI
BbeI BcgI BclI BglII BlpI Bme1580I BmgBI BmtI
BplI BpmI BsaBI BsaHI BsiEI BsiWI BsmBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI FseI
FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI
ZraI