Restriction map for
pFN21AB0445
(Linear) (Six-base) MAPSORT of: KIBB0445.seq Check: 4064 from: 1 to: 1473
KIBB0445.orf
With 182 enzymes: SgfI *
March 22, 2012 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 117 313 1473
Size: 117 196 1160
Fragments arranged by size:
1160 196 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1209 1473
Size: 208 1001 264
Fragments arranged by size:
1001 264 208
AflIII A'CryG_T
Cuts at: 0 380 926 1473
Size: 380 546 547
Fragments arranged by size:
547 546 380
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 1473
Size: 763 32 678
Fragments arranged by size:
763 678 32
AlwNI CAG_nnn'CTG
Cuts at: 0 393 405 462 939 1116 1377 1473
Size: 393 12 57 477 177 261 96
Fragments arranged by size:
477 393 261 177 96 57 12
ApaI G_GGCC'C
Cuts at: 0 425 469 1473
Size: 425 44 1004
Fragments arranged by size:
1004 425 44
ApaLI G'TGCA_C
Cuts at: 0 1316 1473
Size: 1316 157
ApoI r'AATT_y
Cuts at: 0 652 1473
Size: 652 821
AvaI C'yCGr_G
Cuts at: 0 248 738 1350 1473
Size: 248 490 612 123
Fragments arranged by size:
612 490 248 123
BanI G'GyrC_C
Cuts at: 0 786 1473
Size: 786 687
BanII G_rGCy'C
Cuts at: 0 20 89 425 469 1157 1331 1473
Size: 20 69 336 44 688 174 142
Fragments arranged by size:
688 336 174 142 69 44 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 1473
Size: 680 793
BbvCI CC'TCA_GC
Cuts at: 0 1051 1473
Size: 1051 422
BglI GCCn_nnn'nGGC
Cuts at: 0 47 259 1061 1382 1473
Size: 47 212 802 321 91
Fragments arranged by size:
802 321 212 91 47
BlpI GC'TnA_GC
Cuts at: 0 28 442 1156 1473
Size: 28 414 714 317
Fragments arranged by size:
714 414 317 28
Bme1580I G_kGCm'C
Cuts at: 0 425 469 791 1320 1433 1473
Size: 425 44 322 529 113 40
Fragments arranged by size:
529 425 322 113 44 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 1307 1449 1473
Size: 1307 142 24
Fragments arranged by size:
1307 142 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 859 1171 1354 1473
Size: 741 118 312 183 119
Fragments arranged by size:
741 312 183 119 118
Bpu10I CC'TnA_GC
Cuts at: 0 417 1010 1051 1473
Size: 417 593 41 422
Fragments arranged by size:
593 422 417 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1315 1473
Size: 133 1182 158
Fragments arranged by size:
1182 158 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 209 1473
Size: 209 1264
BsaAI yAC'GTr
Cuts at: 0 815 1473
Size: 815 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 128 145 158 265 295 1473
Size: 115 13 17 13 107 30 1178
Fragments arranged by size:
1178 115 107 30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 682 1456 1473
Size: 136 546 774 17
Fragments arranged by size:
774 546 136 17
BseYI C'CCAG_C
Cuts at: 0 1059 1473
Size: 1059 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 949 1153 1473
Size: 391 558 204 320
Fragments arranged by size:
558 391 320 204
BsiHKAI G_wGCw'C
Cuts at: 0 29 372 1157 1320 1331 1473
Size: 29 343 785 163 11 142
Fragments arranged by size:
785 343 163 142 29 11
Bsp1286I G_dGCh'C
Cuts at: 0 20 29 89 372 425 469 791 1157
Size: 20 9 60 283 53 44 322 366
Cuts at: 1157 1320 1331 1433 1473
Size: 163 11 102 40
Fragments arranged by size:
366 322 283 163 102 60 53 44
40 20 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 313 509 1100 1473
Size: 117 196 196 591 373
Fragments arranged by size:
591 373 196 196 117
BsrDI GCAATG_nn'
Cuts at: 0 1193 1473
Size: 1193 280
BsrFI r'CCGG_y
Cuts at: 0 95 1473
Size: 95 1378
BstAPI GCAn_nnn'nTGC
Cuts at: 0 367 393 402 1473
Size: 367 26 9 1071
Fragments arranged by size:
1071 367 26 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 1473
Size: 169 1304
BstYI r'GATC_y
Cuts at: 0 851 1100 1473
Size: 851 249 373
Fragments arranged by size:
851 373 249
BtgI C'CryG_G
Cuts at: 0 80 632 791 1321 1392 1436 1473
Size: 80 552 159 530 71 44 37
Fragments arranged by size:
552 530 159 80 71 44 37
BtsI GCAGTG_nn'
Cuts at: 0 266 1473
Size: 266 1207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 897 932 1176 1211 1473
Size: 897 35 244 35 262
Fragments arranged by size:
897 262 244 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 367 1473
Size: 367 1106
EaeI y'GGCC_r
Cuts at: 0 174 941 1043 1184 1473
Size: 174 767 102 141 289
Fragments arranged by size:
767 289 174 141 102
EarI CTCTTCn'nnn_
Cuts at: 0 184 1252 1288 1473
Size: 184 1068 36 185
Fragments arranged by size:
1068 185 184 36
EcoICRI GAG'CTC
Cuts at: 0 1155 1329 1473
Size: 1155 174 144
Fragments arranged by size:
1155 174 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 741 859 1171 1209 1354 1473
Size: 208 533 118 312 38 145 119
Fragments arranged by size:
533 312 208 145 119 118 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 1473
Size: 454 1019
EcoO109I rG'GnC_Cy
Cuts at: 0 49 421 422 459 466 1083 1473
Size: 49 372 1 37 7 617 390
Fragments arranged by size:
617 390 372 49 37 7 1
EcoRI G'AATT_C
Cuts at: 0 652 1473
Size: 652 821
HaeII r_GCGC'y
Cuts at: 0 7 80 349 1473
Size: 7 73 269 1124
Fragments arranged by size:
1124 269 73 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 265 297 503 535 1473
Size: 115 32 118 32 206 32 938
Fragments arranged by size:
938 206 118 115 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1402 1473
Size: 1402 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1473
Size: 504 969
MscI TGG'CCA
Cuts at: 0 176 943 1045 1473
Size: 176 767 102 428
Fragments arranged by size:
767 428 176 102
MslI CAynn'nnrTG
Cuts at: 0 1198 1473
Size: 1198 275
MspA1I CmG'CkG
Cuts at: 0 32 822 1059 1323 1394 1473
Size: 32 790 237 264 71 79
Fragments arranged by size:
790 264 237 79 71 32
NaeI GCC'GGC
Cuts at: 0 97 1473
Size: 97 1376
NcoI C'CATG_G
Cuts at: 0 80 1436 1473
Size: 80 1356 37
Fragments arranged by size:
1356 80 37
NgoMIV G'CCGG_C
Cuts at: 0 95 1473
Size: 95 1378
NspI r_CATG'y
Cuts at: 0 1212 1473
Size: 1212 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 462 939 1473
Size: 243 219 477 534
Fragments arranged by size:
534 477 243 219
PfoI T'CCnGG_A
Cuts at: 0 533 1473
Size: 533 940
PpuMI rG'GwC_Cy
Cuts at: 0 459 1473
Size: 459 1014
PspOMI G'GGCC_C
Cuts at: 0 421 465 1473
Size: 421 44 1008
Fragments arranged by size:
1008 421 44
PstI C_TGCA'G
Cuts at: 0 211 506 1070 1473
Size: 211 295 564 403
Fragments arranged by size:
564 403 295 211
PvuII CAG'CTG
Cuts at: 0 32 1059 1473
Size: 32 1027 414
Fragments arranged by size:
1027 414 32
SacI G_AGCT'C
Cuts at: 0 1157 1331 1473
Size: 1157 174 142
Fragments arranged by size:
1157 174 142
SacII CC_GC'GG
Cuts at: 0 1324 1395 1473
Size: 1324 71 78
Fragments arranged by size:
1324 78 71
SbfI CC_TGCA'GG
Cuts at: 0 1070 1473
Size: 1070 403
SexAI A'CCwGG_T
Cuts at: 0 1037 1473
Size: 1037 436
SfcI C'TryA_G
Cuts at: 0 207 502 1066 1473
Size: 207 295 564 407
Fragments arranged by size:
564 407 295 207
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 259 1473
Size: 259 1214
SmaI CCC'GGG
Cuts at: 0 250 1352 1473
Size: 250 1102 121
Fragments arranged by size:
1102 250 121
SmlI C'TyrA_G
Cuts at: 0 148 1330 1473
Size: 148 1182 143
Fragments arranged by size:
1182 148 143
SrfI GCCC'GGGC
Cuts at: 0 250 1473
Size: 250 1223
StuI AGG'CCT
Cuts at: 0 1274 1473
Size: 1274 199
StyI C'CwwG_G
Cuts at: 0 80 89 995 1436 1473
Size: 80 9 906 441 37
Fragments arranged by size:
906 441 80 37 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 925 983 1473
Size: 609 316 58 490
Fragments arranged by size:
609 490 316 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 1260 1292 1473
Size: 128 32 1100 32 181
Fragments arranged by size:
1100 181 128 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 169 977 1473
Size: 87 82 808 496
Fragments arranged by size:
808 496 87 82
XmaI C'CCGG_G
Cuts at: 0 248 1350 1473
Size: 248 1102 123
Fragments arranged by size:
1102 248 123
XmnI GAAnn'nnTTC
Cuts at: 0 1384 1473
Size: 1384 89
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII
SbfI SexAI SfcI SfiI SmaI SmlI SrfI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TatI Tth111I XbaI XhoI ZraI