Restriction map for
pFN21AB0444
(Linear) (Six-base) MAPSORT of: KIBB0444.seq Check: 4554 from: 1 to: 2715
KIBB0444.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 289 984 2715
Size: 289 695 1731
Fragments arranged by size:
1731 695 289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 902 1094 1427 1477 2077 2524 2715
Size: 731 171 192 333 50 600 447 191
Fragments arranged by size:
731 600 447 333 192 191 171 50
AhdI GACnn_n'nnGTC
Cuts at: 0 2252 2715
Size: 2252 463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1366 2715
Size: 1366 1349
AlwNI CAG_nnn'CTG
Cuts at: 0 43 215 637 976 2715
Size: 43 172 422 339 1739
Fragments arranged by size:
1739 422 339 172 43
ApoI r'AATT_y
Cuts at: 0 104 430 859 1027 1576 1603 2228 2407
Size: 104 326 429 168 549 27 625 179
Cuts at: 2407 2715
Size: 308
Fragments arranged by size:
625 549 429 326 308 179 168 104
27
AvaI C'yCGr_G
Cuts at: 0 1057 2715
Size: 1057 1658
BanI G'GyrC_C
Cuts at: 0 1276 1359 2652 2715
Size: 1276 83 1293 63
Fragments arranged by size:
1293 1276 83 63
BbeI G_GCGC'C
Cuts at: 0 1280 1363 2656 2715
Size: 1280 83 1293 59
Fragments arranged by size:
1293 1280 83 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 1661 1924 2612 2715
Size: 1661 263 688 103
Fragments arranged by size:
1661 688 263 103
BclI T'GATC_A
Cuts at: 0 1733 2715
Size: 1733 982
BfrBI ATG'CAT
Cuts at: 0 2660 2715
Size: 2660 55
BglI GCCn_nnn'nGGC
Cuts at: 0 1336 2287 2715
Size: 1336 951 428
Fragments arranged by size:
1336 951 428
BglII A'GATC_T
Cuts at: 0 2358 2715
Size: 2358 357
Bme1580I G_kGCm'C
Cuts at: 0 1200 1229 2715
Size: 1200 29 1486
Fragments arranged by size:
1486 1200 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1223 2361 2715
Size: 1223 1138 354
Fragments arranged by size:
1223 1138 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 2715
Size: 20 2695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1948 2715
Size: 1948 767
BsaAI yAC'GTr
Cuts at: 0 1350 2715
Size: 1350 1365
BsaBI GATnn'nnATC
Cuts at: 0 1428 2715
Size: 1428 1287
BsaHI Gr'CG_yC
Cuts at: 0 1277 1360 2653 2715
Size: 1277 83 1293 62
Fragments arranged by size:
1293 1277 83 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 598 2715
Size: 108 490 2117
Fragments arranged by size:
2117 490 108
BseYI C'CCAG_C
Cuts at: 0 758 2715
Size: 758 1957
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1706 2715
Size: 460 1246 1009
Fragments arranged by size:
1246 1009 460
BsiHKAI G_wGCw'C
Cuts at: 0 1249 2715
Size: 1249 1466
BsmI GAATG_Cn'
Cuts at: 0 2283 2715
Size: 2283 432
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2479 2715
Size: 2479 236
Bsp1286I G_dGCh'C
Cuts at: 0 1200 1229 1249 2715
Size: 1200 29 20 1466
Fragments arranged by size:
1466 1200 29 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 984 1383 1844 2715
Size: 289 695 399 461 871
Fragments arranged by size:
871 695 461 399 289
BsrDI GCAATG_nn'
Cuts at: 0 1784 2715
Size: 1784 931
BsrFI r'CCGG_y
Cuts at: 0 2702 2715
Size: 2702 13
BsrGI T'GTAC_A
Cuts at: 0 1700 2715
Size: 1700 1015
BssSI C'ACGA_G
Cuts at: 0 1199 2715
Size: 1199 1516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2688 2715
Size: 2688 27
BstYI r'GATC_y
Cuts at: 0 2358 2715
Size: 2358 357
Bsu36I CC'TnA_GG
Cuts at: 0 1718 2715
Size: 1718 997
BtgI C'CryG_G
Cuts at: 0 1955 2715
Size: 1955 760
BtsI GCAGTG_nn'
Cuts at: 0 906 1043 1098 2212 2715
Size: 906 137 55 1114 503
Fragments arranged by size:
1114 906 503 137 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2686 2715
Size: 2686 29
DraI TTT'AAA
Cuts at: 0 1581 2715
Size: 1581 1134
DraIII CAC_nnn'GTG
Cuts at: 0 1395 1591 2715
Size: 1395 196 1124
Fragments arranged by size:
1395 1124 196
EaeI y'GGCC_r
Cuts at: 0 701 872 1040 2715
Size: 701 171 168 1675
Fragments arranged by size:
1675 701 171 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 731 902 1094 1427 1477 2077 2524
Size: 20 711 171 192 333 50 600 447
Cuts at: 2524 2715
Size: 191
Fragments arranged by size:
711 600 447 333 192 191 171 50
20
EcoO109I rG'GnC_Cy
Cuts at: 0 802 1307 2715
Size: 802 505 1408
Fragments arranged by size:
1408 802 505
EcoRV GAT'ATC
Cuts at: 0 1714 2715
Size: 1714 1001
HaeII r_GCGC'y
Cuts at: 0 1280 1363 2656 2715
Size: 1280 83 1293 59
Fragments arranged by size:
1293 1280 83 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 1711 1743 1968 2000 2238 2270
Size: 11 32 1668 32 225 32 238 32
Cuts at: 2270 2715
Size: 445
Fragments arranged by size:
1668 445 238 225 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 1839 2715
Size: 1839 876
HindIII A'AGCT_T
Cuts at: 0 1177 2715
Size: 1177 1538
KasI G'GCGC_C
Cuts at: 0 1276 1359 2652 2715
Size: 1276 83 1293 63
Fragments arranged by size:
1293 1276 83 63
MfeI C'AATT_G
Cuts at: 0 2129 2715
Size: 2129 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 2715
Size: 1169 1546
MscI TGG'CCA
Cuts at: 0 703 874 1042 2715
Size: 703 171 168 1673
Fragments arranged by size:
1673 703 171 168
MspA1I CmG'CkG
Cuts at: 0 543 1405 2715
Size: 543 862 1310
Fragments arranged by size:
1310 862 543
NarI GG'CG_CC
Cuts at: 0 1277 1360 2653 2715
Size: 1277 83 1293 62
Fragments arranged by size:
1293 1277 83 62
NsiI A_TGCA'T
Cuts at: 0 2662 2715
Size: 2662 53
NspI r_CATG'y
Cuts at: 0 457 2715
Size: 457 2258
PflMI CCAn_nnn'nTGG
Cuts at: 0 2198 2715
Size: 2198 517
PmlI CAC'GTG
Cuts at: 0 1350 2715
Size: 1350 1365
PpuMI rG'GwC_Cy
Cuts at: 0 802 2715
Size: 802 1913
PsiI TTA'TAA
Cuts at: 0 983 2715
Size: 983 1732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 389 421 2715
Size: 389 32 2294
Fragments arranged by size:
2294 389 32
PstI C_TGCA'G
Cuts at: 0 963 1437 2562 2715
Size: 963 474 1125 153
Fragments arranged by size:
1125 963 474 153
PvuII CAG'CTG
Cuts at: 0 1405 2715
Size: 1405 1310
ScaI AGT'ACT
Cuts at: 0 2685 2715
Size: 2685 30
SfcI C'TryA_G
Cuts at: 0 959 1433 2558 2715
Size: 959 474 1125 157
Fragments arranged by size:
1125 959 474 157
SfoI GGC'GCC
Cuts at: 0 1278 1361 2654 2715
Size: 1278 83 1293 61
Fragments arranged by size:
1293 1278 83 61
SmlI C'TyrA_G
Cuts at: 0 1057 1963 2715
Size: 1057 906 752
Fragments arranged by size:
1057 906 752
SspI AAT'ATT
Cuts at: 0 333 2715
Size: 333 2382
StuI AGG'CCT
Cuts at: 0 1330 2715
Size: 1330 1385
StyI C'CwwG_G
Cuts at: 0 1363 1902 2291 2697 2715
Size: 1363 539 389 406 18
Fragments arranged by size:
1363 539 406 389 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2580 2715
Size: 2580 135
TatI w'GTAC_w
Cuts at: 0 1117 1150 1700 2683 2715
Size: 1117 33 550 983 32
Fragments arranged by size:
1117 983 550 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 878 910 1070 1102 2715
Size: 878 32 160 32 1613
Fragments arranged by size:
1613 878 160 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1900 2715
Size: 1900 815
XhoI C'TCGA_G
Cuts at: 0 1057 2715
Size: 1057 1658
XmnI GAAnn'nnTTC
Cuts at: 0 1658 1828 2051 2715
Size: 1658 170 223 664
Fragments arranged by size:
1658 664 223 170
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApoI AvaI BanI
BbeI BbsI BclI BfrBI BglI BglII Bme1580I BmrI
BpmI BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII EaeI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII
HindIII KasI MfeI MmeI MscI MspA1I NarI NsiI
NspI PflMI PmlI PpuMI PsiI PsrI PstI PvuII
ScaI SfcI SfoI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbvCI BcgI BciVI BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaWI BsaXI BsiEI BsiWI
BspEI BspHI BsrBI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI ClaI DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HpaI KpnI MluI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PpiI PshAI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XmaI ZraI