Restriction map for
pFN21AB0443
(Linear) (Six-base) MAPSORT of: KIBB0443.seq Check: 7754 from: 1 to: 1116
KIBB0443.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AatII G_ACGT'C
Cuts at: 0 1000 1116
Size: 1000 116
Acc65I G'GTAC_C
Cuts at: 0 578 1116
Size: 578 538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 193 504 508 856 1116
Size: 160 33 311 4 348 260
Fragments arranged by size:
348 311 260 160 33 4
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 344 1116
Size: 344 772
AlwNI CAG_nnn'CTG
Cuts at: 0 396 1116
Size: 396 720
AvaI C'yCGr_G
Cuts at: 0 512 601 1116
Size: 512 89 515
Fragments arranged by size:
515 512 89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 562 595 1116
Size: 562 33 521
Fragments arranged by size:
562 521 33
BanI G'GyrC_C
Cuts at: 0 82 110 578 1017 1116
Size: 82 28 468 439 99
Fragments arranged by size:
468 439 99 82 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1104 1116
Size: 1104 12
BglI GCCn_nnn'nGGC
Cuts at: 0 1034 1116
Size: 1034 82
Bme1580I G_kGCm'C
Cuts at: 0 31 1116
Size: 31 1085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1116
Size: 814 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1116
Size: 955 161
BsaBI GATnn'nnATC
Cuts at: 0 202 1116
Size: 202 914
BsaHI Gr'CG_yC
Cuts at: 0 923 997 1116
Size: 923 74 119
Fragments arranged by size:
923 119 74
BseYI C'CCAG_C
Cuts at: 0 331 643 902 1049 1116
Size: 331 312 259 147 67
Fragments arranged by size:
331 312 259 147 67
Bsp1286I G_dGCh'C
Cuts at: 0 31 1116
Size: 31 1085
BsrBI CCG'CTC
Cuts at: 0 609 1116
Size: 609 507
BsrDI GCAATG_nn'
Cuts at: 0 351 1116
Size: 351 765
BsrFI r'CCGG_y
Cuts at: 0 121 581 1116
Size: 121 460 535
Fragments arranged by size:
535 460 121
BssSI C'ACGA_G
Cuts at: 0 223 473 1116
Size: 223 250 643
Fragments arranged by size:
643 250 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 1116
Size: 396 720
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 1116
Size: 564 552
BstYI r'GATC_y
Cuts at: 0 203 431 1116
Size: 203 228 685
Fragments arranged by size:
685 228 203
Bsu36I CC'TnA_GG
Cuts at: 0 749 1116
Size: 749 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 932 1116
Size: 932 184
EaeI y'GGCC_r
Cuts at: 0 75 123 527 757 1116
Size: 75 48 404 230 359
Fragments arranged by size:
404 359 230 75 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 193 504 508 814 856 1116
Size: 160 33 311 4 306 42 260
Fragments arranged by size:
311 306 260 160 42 33 4
EcoO109I rG'GnC_Cy
Cuts at: 0 483 1116
Size: 483 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1116
Size: 1059 32 25
Fragments arranged by size:
1059 32 25
HincII GTy'rAC
Cuts at: 0 237 1116
Size: 237 879
HindIII A'AGCT_T
Cuts at: 0 263 1116
Size: 263 853
KpnI G_GTAC'C
Cuts at: 0 582 1116
Size: 582 534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 989 1116
Size: 974 15 127
Fragments arranged by size:
974 127 15
MscI TGG'CCA
Cuts at: 0 529 759 1116
Size: 529 230 357
Fragments arranged by size:
529 357 230
MslI CAynn'nnrTG
Cuts at: 0 227 1116
Size: 227 889
MspA1I CmG'CkG
Cuts at: 0 281 1116
Size: 281 835
NspI r_CATG'y
Cuts at: 0 981 1116
Size: 981 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 1116
Size: 132 984
PpuMI rG'GwC_Cy
Cuts at: 0 483 1116
Size: 483 633
ScaI AGT'ACT
Cuts at: 0 1012 1116
Size: 1012 104
SexAI A'CCwGG_T
Cuts at: 0 299 1116
Size: 299 817
SmaI CCC'GGG
Cuts at: 0 514 1116
Size: 514 602
SmlI C'TyrA_G
Cuts at: 0 970 1116
Size: 970 146
SphI G_CATG'C
Cuts at: 0 981 1116
Size: 981 135
SrfI GCCC'GGGC
Cuts at: 0 514 1116
Size: 514 602
StuI AGG'CCT
Cuts at: 0 841 1116
Size: 841 275
StyI C'CwwG_G
Cuts at: 0 78 1116
Size: 78 1038
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 635 1003 1063 1116
Size: 635 368 60 53
Fragments arranged by size:
635 368 60 53
TatI w'GTAC_w
Cuts at: 0 1010 1116
Size: 1010 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1116
Size: 982 32 102
Fragments arranged by size:
982 102 32
XmaI C'CCGG_G
Cuts at: 0 512 1116
Size: 512 604
ZraI GAC'GTC
Cuts at: 0 998 1116
Size: 998 118
Enzymes that do cut:
AatII Acc65I AcuI AlfI AlwNI AvaI BaeI BanI
BbsI BglI Bme1580I BpmI BpuEI BsaBI BsaHI BseYI
Bsp1286I BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgZI EaeI Eco57MI EcoO109I Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NspI PflMI PpuMI
ScaI SexAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII
BstBI BstEII BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI XcmI XhoI XmnI