Restriction map for
pFN21AB0426
(Linear) (Six-base) MAPSORT of: KIBB0426.seq Check: 1322 from: 1 to: 2535
KIBB0426.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 250 2535
Size: 250 2285
Acc65I G'GTAC_C
Cuts at: 0 1612 2535
Size: 1612 923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 2535
Size: 1358 1177
AfeI AGC'GCT
Cuts at: 0 583 2535
Size: 583 1952
AflII C'TTAA_G
Cuts at: 0 214 2535
Size: 214 2321
AflIII A'CryG_T
Cuts at: 0 1406 2535
Size: 1406 1129
AleI CACnn'nnGTG
Cuts at: 0 1237 2535
Size: 1237 1298
AlwNI CAG_nnn'CTG
Cuts at: 0 207 1026 1188 1602 1995 2535
Size: 207 819 162 414 393 540
Fragments arranged by size:
819 540 414 393 207 162
ApoI r'AATT_y
Cuts at: 0 2110 2535
Size: 2110 425
AvaI C'yCGr_G
Cuts at: 0 899 2384 2535
Size: 899 1485 151
Fragments arranged by size:
1485 899 151
BanI G'GyrC_C
Cuts at: 0 11 775 981 1432 1612 1643 2535
Size: 11 764 206 451 180 31 892
Fragments arranged by size:
892 764 451 206 180 31 11
BanII G_rGCy'C
Cuts at: 0 84 278 545 643 1752 1880 2535
Size: 84 194 267 98 1109 128 655
Fragments arranged by size:
1109 655 267 194 128 98 84
BbeI G_GCGC'C
Cuts at: 0 15 2535
Size: 15 2520
BbsI GAAGACnn'nnnn_
Cuts at: 0 422 798 2535
Size: 422 376 1737
Fragments arranged by size:
1737 422 376
BbvCI CC'TCA_GC
Cuts at: 0 1889 2535
Size: 1889 646
BglI GCCn_nnn'nGGC
Cuts at: 0 2486 2535
Size: 2486 49
BglII A'GATC_T
Cuts at: 0 515 2471 2535
Size: 515 1956 64
Fragments arranged by size:
1956 515 64
Bme1580I G_kGCm'C
Cuts at: 0 778 1437 2535
Size: 778 659 1098
Fragments arranged by size:
1098 778 659
BmgBI CAC'GTC
Cuts at: 0 909 2535
Size: 909 1626
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 2535
Size: 195 2340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1700 2535
Size: 1700 835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1003 1850 1883 2535
Size: 943 60 847 33 652
Fragments arranged by size:
943 847 652 60 33
Bpu10I CC'TnA_GC
Cuts at: 0 83 1751 1889 2535
Size: 83 1668 138 646
Fragments arranged by size:
1668 646 138 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 674 1210 1312 2392 2535
Size: 247 427 536 102 1080 143
Fragments arranged by size:
1080 536 427 247 143 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 1373 1567 2381 2535
Size: 1373 194 814 154
Fragments arranged by size:
1373 814 194 154
BsaAI yAC'GTr
Cuts at: 0 2508 2535
Size: 2508 27
BsaBI GATnn'nnATC
Cuts at: 0 1504 2535
Size: 1504 1031
BsaHI Gr'CG_yC
Cuts at: 0 12 2535
Size: 12 2523
BsaWI w'CCGG_w
Cuts at: 0 1521 2180 2535
Size: 1521 659 355
Fragments arranged by size:
1521 659 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 607 637 1856 1886 2535
Size: 532 30 45 30 1219 30 649
Fragments arranged by size:
1219 649 532 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 412 475 511 631 1585 1976 2535
Size: 409 3 63 36 120 954 391 559
Fragments arranged by size:
954 559 409 391 120 63 36 3
BseYI C'CCAG_C
Cuts at: 0 55 636 1773 2535
Size: 55 581 1137 762
Fragments arranged by size:
1137 762 581 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 931 1330 1640 2535
Size: 325 606 399 310 895
Fragments arranged by size:
895 606 399 325 310
BsiEI CG_ry'CG
Cuts at: 0 359 2535
Size: 359 2176
BsiHKAI G_wGCw'C
Cuts at: 0 278 1880 2535
Size: 278 1602 655
Fragments arranged by size:
1602 655 278
BsmI GAATG_Cn'
Cuts at: 0 1533 2535
Size: 1533 1002
Bsp1286I G_dGCh'C
Cuts at: 0 84 278 545 643 778 1437 1752 1880
Size: 84 194 267 98 135 659 315 128
Cuts at: 1880 2535
Size: 655
Fragments arranged by size:
659 655 315 267 194 135 128 98
84
BspEI T'CCGG_A
Cuts at: 0 1521 2535
Size: 1521 1014
BspHI T'CATG_A
Cuts at: 0 2159 2535
Size: 2159 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 176 191 250 734 980 1073 1352
Size: 155 21 15 59 484 246 93 279
Cuts at: 1352 2535
Size: 1183
Fragments arranged by size:
1183 484 279 246 155 93 59 21
15
BsrBI CCG'CTC
Cuts at: 0 2031 2063 2535
Size: 2031 32 472
Fragments arranged by size:
2031 472 32
BsrFI r'CCGG_y
Cuts at: 0 1202 2535
Size: 1202 1333
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2113 2535
Size: 2113 422
BstBI TT'CG_AA
Cuts at: 0 281 2535
Size: 281 2254
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2508 2535
Size: 2508 27
BstYI r'GATC_y
Cuts at: 0 515 602 2429 2471 2535
Size: 515 87 1827 42 64
Fragments arranged by size:
1827 515 87 64 42
Bsu36I CC'TnA_GG
Cuts at: 0 1988 2535
Size: 1988 547
BtgI C'CryG_G
Cuts at: 0 1977 2024 2535
Size: 1977 47 511
Fragments arranged by size:
1977 511 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1063 1726 2535
Size: 1063 663 809
Fragments arranged by size:
1063 809 663
BtsI GCAGTG_nn'
Cuts at: 0 1135 2336 2535
Size: 1135 1201 199
Fragments arranged by size:
1201 1135 199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2440 2475 2535
Size: 2440 35 60
Fragments arranged by size:
2440 60 35
DraIII CAC_nnn'GTG
Cuts at: 0 66 2535
Size: 66 2469
EaeI y'GGCC_r
Cuts at: 0 840 890 1200 1502 1692 2054 2478 2535
Size: 840 50 310 302 190 362 424 57
Fragments arranged by size:
840 424 362 310 302 190 57 50
EarI CTCTTCn'nnn_
Cuts at: 0 283 301 1377 2535
Size: 283 18 1076 1158
Fragments arranged by size:
1158 1076 283 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 517 2535
Size: 517 2018
EcoICRI GAG'CTC
Cuts at: 0 276 1878 2535
Size: 276 1602 657
Fragments arranged by size:
1602 657 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1003 1358 1850 1883 2535
Size: 943 60 355 492 33 652
Fragments arranged by size:
943 652 492 355 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1018 1420 2535
Size: 172 846 402 1115
Fragments arranged by size:
1115 846 402 172
EcoO109I rG'GnC_Cy
Cuts at: 0 105 471 525 723 969 1092 1680 1992
Size: 105 366 54 198 246 123 588 312
Cuts at: 1992 2020 2535
Size: 28 515
Fragments arranged by size:
588 515 366 312 246 198 123 105
54 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2274 2535
Size: 2274 261
FseI GG_CCGG'CC
Cuts at: 0 1206 2535
Size: 1206 1329
HaeII r_GCGC'y
Cuts at: 0 15 585 1251 2535
Size: 15 570 666 1284
Fragments arranged by size:
1284 666 570 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 1564 1596 2456 2488 2535
Size: 532 32 1000 32 860 32 47
Fragments arranged by size:
1000 860 532 47 32 32 32
HincII GTy'rAC
Cuts at: 0 180 2535
Size: 180 2355
KasI G'GCGC_C
Cuts at: 0 11 2535
Size: 11 2524
KpnI G_GTAC'C
Cuts at: 0 1616 2535
Size: 1616 919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1535 2535
Size: 461 1074 1000
Fragments arranged by size:
1074 1000 461
MscI TGG'CCA
Cuts at: 0 1504 2056 2535
Size: 1504 552 479
Fragments arranged by size:
1504 552 479
MslI CAynn'nnrTG
Cuts at: 0 1237 2535
Size: 1237 1298
MspA1I CmG'CkG
Cuts at: 0 135 1161 1634 2535
Size: 135 1026 473 901
Fragments arranged by size:
1026 901 473 135
NaeI GCC'GGC
Cuts at: 0 1204 2535
Size: 1204 1331
NarI GG'CG_CC
Cuts at: 0 12 2535
Size: 12 2523
NcoI C'CATG_G
Cuts at: 0 1977 2024 2535
Size: 1977 47 511
Fragments arranged by size:
1977 511 47
NgoMIV G'CCGG_C
Cuts at: 0 1202 2535
Size: 1202 1333
NspI r_CATG'y
Cuts at: 0 1410 2535
Size: 1410 1125
PasI CC'CwG_GG
Cuts at: 0 771 1437 2535
Size: 771 666 1098
Fragments arranged by size:
1098 771 666
PciI A'CATG_T
Cuts at: 0 1406 2535
Size: 1406 1129
PflMI CCAn_nnn'nTGG
Cuts at: 0 1026 1188 1828 2535
Size: 1026 162 640 707
Fragments arranged by size:
1026 707 640 162
PfoI T'CCnGG_A
Cuts at: 0 110 346 1019 2535
Size: 110 236 673 1516
Fragments arranged by size:
1516 673 236 110
PpuMI rG'GwC_Cy
Cuts at: 0 471 525 723 969 1092 1680 1992 2535
Size: 471 54 198 246 123 588 312 543
Fragments arranged by size:
588 543 471 312 246 198 123 54
PstI C_TGCA'G
Cuts at: 0 853 2535
Size: 853 1682
PvuI CG_AT'CG
Cuts at: 0 359 2535
Size: 359 2176
PvuII CAG'CTG
Cuts at: 0 135 1161 2535
Size: 135 1026 1374
Fragments arranged by size:
1374 1026 135
SacI G_AGCT'C
Cuts at: 0 278 1880 2535
Size: 278 1602 655
Fragments arranged by size:
1602 655 278
SanDI GG'GwC_CC
Cuts at: 0 1680 2535
Size: 1680 855
SapI GCTCTTCn'nnn_
Cuts at: 0 283 2535
Size: 283 2252
SexAI A'CCwGG_T
Cuts at: 0 800 2535
Size: 800 1735
SfcI C'TryA_G
Cuts at: 0 423 849 2535
Size: 423 426 1686
Fragments arranged by size:
1686 426 423
SfoI GGC'GCC
Cuts at: 0 13 2535
Size: 13 2522
SmaI CCC'GGG
Cuts at: 0 901 2535
Size: 901 1634
SmlI C'TyrA_G
Cuts at: 0 214 262 689 1225 1327 2407 2535
Size: 214 48 427 536 102 1080 128
Fragments arranged by size:
1080 536 427 214 128 102 48
StuI AGG'CCT
Cuts at: 0 1591 2535
Size: 1591 944
StyI C'CwwG_G
Cuts at: 0 1428 1977 2024 2535
Size: 1428 549 47 511
Fragments arranged by size:
1428 549 511 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1397 2405 2535
Size: 37 1360 1008 130
Fragments arranged by size:
1360 1008 130 37
TatI w'GTAC_w
Cuts at: 0 623 2535
Size: 623 1912
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1856 1888 2535
Size: 1856 32 647
Fragments arranged by size:
1856 647 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1092 1173 2535
Size: 876 216 81 1362
Fragments arranged by size:
1362 876 216 81
XmaI C'CCGG_G
Cuts at: 0 899 2535
Size: 899 1636
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AflIII AleI AlwNI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PstI PvuI PvuII SacI SanDI SapI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BlpI BmtI BsiWI BsmBI BsrDI
BsrGI BssHII BssSI BstEII BstZ17I ClaI DraI DrdI
EagI EcoRI EcoRV FspI FspAI HindIII HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI RsrII
SacII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI