Restriction map for
pFN21AB0120
(Linear) (Six-base) MAPSORT of: KIBB0120.seq Check: 6350 from: 1 to: 4587
KIBB0120.orf
With 182 enzymes: SgfI *
July 3, 2012 15:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4072 4159 4587
Size: 4072 87 428
Fragments arranged by size:
4072 428 87
AccI GT'mk_AC
Cuts at: 0 851 3082 4587
Size: 851 2231 1505
Fragments arranged by size:
2231 1505 851
AclI AA'CG_TT
Cuts at: 0 2993 4587
Size: 2993 1594
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 328 1207 2373 2543 3496 3506 3541
Size: 235 93 879 1166 170 953 10 35
Cuts at: 3541 4587
Size: 1046
Fragments arranged by size:
1166 1046 953 879 235 170 93 35
10
AflII C'TTAA_G
Cuts at: 0 2349 4587
Size: 2349 2238
AflIII A'CryG_T
Cuts at: 0 2794 3034 4587
Size: 2794 240 1553
Fragments arranged by size:
2794 1553 240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 836 4171 4418 4587
Size: 836 3335 247 169
Fragments arranged by size:
3335 836 247 169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 4587
Size: 1186 32 3369
Fragments arranged by size:
3369 1186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 24 3129 3371 3679 4587
Size: 24 3105 242 308 908
Fragments arranged by size:
3105 908 308 242 24
ApaI G_GGCC'C
Cuts at: 0 715 4094 4587
Size: 715 3379 493
Fragments arranged by size:
3379 715 493
ApoI r'AATT_y
Cuts at: 0 413 462 760 1050 1423 1630 2710 3486
Size: 413 49 298 290 373 207 1080 776
Cuts at: 3486 3819 4587
Size: 333 768
Fragments arranged by size:
1080 776 768 413 373 333 298 290
207 49
AseI AT'TA_AT
Cuts at: 0 3050 4587
Size: 3050 1537
AvaI C'yCGr_G
Cuts at: 0 102 683 4587
Size: 102 581 3904
Fragments arranged by size:
3904 581 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2195 2228 3192 3225 4587
Size: 2195 33 964 33 1362
Fragments arranged by size:
2195 1362 964 33 33
BanI G'GyrC_C
Cuts at: 0 69 1360 3966 4147 4587
Size: 69 1291 2606 181 440
Fragments arranged by size:
2606 1291 440 181 69
BanII G_rGCy'C
Cuts at: 0 519 715 3267 3402 4094 4587
Size: 519 196 2552 135 692 493
Fragments arranged by size:
2552 692 519 493 196 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 3581 4587
Size: 220 3361 1006
Fragments arranged by size:
3361 1006 220
BbvCI CC'TCA_GC
Cuts at: 0 4483 4587
Size: 4483 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 862 4424 4458 4587
Size: 828 34 3562 34 129
Fragments arranged by size:
3562 828 129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2196 4315 4587
Size: 2196 2119 272
Fragments arranged by size:
2196 2119 272
BfrBI ATG'CAT
Cuts at: 0 1275 4587
Size: 1275 3312
BglI GCCn_nnn'nGGC
Cuts at: 0 4055 4587
Size: 4055 532
BglII A'GATC_T
Cuts at: 0 1004 4587
Size: 1004 3583
Bme1580I G_kGCm'C
Cuts at: 0 85 149 715 1365 3123 4026 4094 4587
Size: 85 64 566 650 1758 903 68 493
Fragments arranged by size:
1758 903 650 566 493 85 68 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 1590 2625 4587
Size: 1590 1035 1962
Fragments arranged by size:
1962 1590 1035
BmtI G_CTAG'C
Cuts at: 0 1324 4587
Size: 1324 3263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4228 4587
Size: 4228 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 2687 4587
Size: 971 1716 1900
Fragments arranged by size:
1900 1716 971
Bpu10I CC'TnA_GC
Cuts at: 0 991 4483 4587
Size: 991 3492 104
Fragments arranged by size:
3492 991 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 4587
Size: 1297 3290
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 892 1345 4587
Size: 874 18 453 3242
Fragments arranged by size:
3242 874 453 18
BsaAI yAC'GTr
Cuts at: 0 1681 3204 3296 4587
Size: 1681 1523 92 1291
Fragments arranged by size:
1681 1523 1291 92
BsaWI w'CCGG_w
Cuts at: 0 3923 4587
Size: 3923 664
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 4219 4249 4513 4543 4587
Size: 1186 30 3003 30 264 30 44
Fragments arranged by size:
3003 1186 264 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 1735 2398 2441 3115 4240 4500 4518
Size: 302 1433 663 43 674 1125 260 18
Cuts at: 4518 4587
Size: 69
Fragments arranged by size:
1433 1125 674 663 302 260 69 43
18
BseYI C'CCAG_C
Cuts at: 0 36 2402 4042 4587
Size: 36 2366 1640 545
Fragments arranged by size:
2366 1640 545 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1810 3174 3255 4296 4464 4478 4587
Size: 1810 1364 81 1041 168 14 109
Fragments arranged by size:
1810 1364 1041 168 109 81 14
BsiHKAI G_wGCw'C
Cuts at: 0 97 539 1589 3402 4587
Size: 97 442 1050 1813 1185
Fragments arranged by size:
1813 1185 1050 442 97
BsmI GAATG_Cn'
Cuts at: 0 292 508 618 1143 4315 4519 4587
Size: 292 216 110 525 3172 204 68
Fragments arranged by size:
3172 525 292 216 204 110 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1621 4587
Size: 1621 2966
Bsp1286I G_dGCh'C
Cuts at: 0 85 97 149 519 539 715 1365 1589
Size: 85 12 52 370 20 176 650 224
Cuts at: 1589 3123 3267 3402 4026 4094 4587
Size: 1534 144 135 624 68 493
Fragments arranged by size:
1534 650 624 493 370 224 176 144
135 85 68 52 20 12
BspEI T'CCGG_A
Cuts at: 0 3923 4587
Size: 3923 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4072 4159 4587
Size: 4072 87 428
Fragments arranged by size:
4072 428 87
BsrFI r'CCGG_y
Cuts at: 0 3661 4587
Size: 3661 926
BsrGI T'GTAC_A
Cuts at: 0 695 4587
Size: 695 3892
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1024 4587
Size: 1024 3563
BstBI TT'CG_AA
Cuts at: 0 4511 4587
Size: 4511 76
BstEII G'GTnAC_C
Cuts at: 0 775 4587
Size: 775 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3889 4587
Size: 3889 698
BstYI r'GATC_y
Cuts at: 0 300 878 1004 1179 1506 4230 4587
Size: 300 578 126 175 327 2724 357
Fragments arranged by size:
2724 578 357 327 300 175 126
BstZ17I GTA'TAC
Cuts at: 0 3083 4587
Size: 3083 1504
BtgI C'CryG_G
Cuts at: 0 441 3347 3764 3912 4026 4200 4587
Size: 441 2906 417 148 114 174 387
Fragments arranged by size:
2906 441 417 387 174 148 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1215 4587
Size: 1215 3372
BtsI GCAGTG_nn'
Cuts at: 0 97 777 813 1601 2608 3213 3267 3287
Size: 97 680 36 788 1007 605 54 20
Cuts at: 3287 4323 4474 4587
Size: 1036 151 113
Fragments arranged by size:
1036 1007 788 680 605 151 113 97
54 36 20
ClaI AT'CG_AT
Cuts at: 0 1476 3224 4587
Size: 1476 1748 1363
Fragments arranged by size:
1748 1476 1363
DraI TTT'AAA
Cuts at: 0 185 4587
Size: 185 4402
DraIII CAC_nnn'GTG
Cuts at: 0 704 3726 4587
Size: 704 3022 861
Fragments arranged by size:
3022 861 704
DrdI GACnn_nn'nnGTC
Cuts at: 0 2863 4587
Size: 2863 1724
EaeI y'GGCC_r
Cuts at: 0 1985 2114 4587
Size: 1985 129 2473
Fragments arranged by size:
2473 1985 129
EarI CTCTTCn'nnn_
Cuts at: 0 1259 2038 2483 3853 4217 4587
Size: 1259 779 445 1370 364 370
Fragments arranged by size:
1370 1259 779 445 370 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4512 4587
Size: 4512 75
EcoICRI GAG'CTC
Cuts at: 0 3400 4587
Size: 3400 1187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 328 971 1207 2373 2543 2687 3496
Size: 235 93 643 236 1166 170 144 809
Cuts at: 3496 3506 3541 4587
Size: 10 35 1046
Fragments arranged by size:
1166 1046 809 643 236 235 170 144
93 35 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 3595 4587
Size: 61 3534 992
Fragments arranged by size:
3534 992 61
EcoO109I rG'GnC_Cy
Cuts at: 0 525 712 1241 2725 3126 3885 4090 4091
Size: 525 187 529 1484 401 759 205 1
Cuts at: 4091 4587
Size: 496
Fragments arranged by size:
1484 759 529 525 496 401 205 187
1
EcoRI G'AATT_C
Cuts at: 0 1050 4587
Size: 1050 3537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 452 1260 3129 4587
Size: 452 808 1869 1458
Fragments arranged by size:
1869 1458 808 452
FspI TGC'GCA
Cuts at: 0 139 4587
Size: 139 4448
HaeII r_GCGC'y
Cuts at: 0 136 4587
Size: 136 4451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 749 781 984 1016 1462 1494 1830 1862
Size: 749 32 203 32 446 32 336 32
Cuts at: 1862 3649 3681 4513 4545 4587
Size: 1787 32 832 32 42
Fragments arranged by size:
1787 832 749 446 336 203 42 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 453 2814 2991 4587
Size: 453 2361 177 1596
Fragments arranged by size:
2361 1596 453 177
HindIII A'AGCT_T
Cuts at: 0 434 973 1219 4587
Size: 434 539 246 3368
Fragments arranged by size:
3368 539 434 246
MfeI C'AATT_G
Cuts at: 0 2564 4587
Size: 2564 2023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1622 2345 2606 2636 4587
Size: 686 936 723 261 30 1951
Fragments arranged by size:
1951 936 723 686 261 30
MscI TGG'CCA
Cuts at: 0 1987 2116 4587
Size: 1987 129 2471
Fragments arranged by size:
2471 1987 129
MslI CAynn'nnrTG
Cuts at: 0 2501 4205 4587
Size: 2501 1704 382
Fragments arranged by size:
2501 1704 382
MspA1I CmG'CkG
Cuts at: 0 348 491 3581 4181 4337 4482 4587
Size: 348 143 3090 600 156 145 105
Fragments arranged by size:
3090 600 348 156 145 143 105
NcoI C'CATG_G
Cuts at: 0 3347 4026 4200 4587
Size: 3347 679 174 387
Fragments arranged by size:
3347 679 387 174
NdeI CA'TA_TG
Cuts at: 0 590 2838 3301 4587
Size: 590 2248 463 1286
Fragments arranged by size:
2248 1286 590 463
NheI G'CTAG_C
Cuts at: 0 1320 4587
Size: 1320 3267
NsiI A_TGCA'T
Cuts at: 0 1277 4587
Size: 1277 3310
NspI r_CATG'y
Cuts at: 0 2601 2798 3038 3065 3852 4037 4587
Size: 2601 197 240 27 787 185 550
Fragments arranged by size:
2601 787 550 240 197 185 27
PasI CC'CwG_GG
Cuts at: 0 3889 4587
Size: 3889 698
PciI A'CATG_T
Cuts at: 0 2794 3034 4587
Size: 2794 240 1553
Fragments arranged by size:
2794 1553 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 2112 3726 4587
Size: 2112 1614 861
Fragments arranged by size:
2112 1614 861
PfoI T'CCnGG_A
Cuts at: 0 296 521 948 1580 2105 2664 4587
Size: 296 225 427 632 525 559 1923
Fragments arranged by size:
1923 632 559 525 427 296 225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 4587
Size: 1186 32 3369
Fragments arranged by size:
3369 1186 32
PpuMI rG'GwC_Cy
Cuts at: 0 525 1241 2725 3126 4587
Size: 525 716 1484 401 1461
Fragments arranged by size:
1484 1461 716 525 401
PsiI TTA'TAA
Cuts at: 0 3493 4587
Size: 3493 1094
PspOMI G'GGCC_C
Cuts at: 0 711 4090 4587
Size: 711 3379 497
Fragments arranged by size:
3379 711 497
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 731 763 4587
Size: 731 32 3824
Fragments arranged by size:
3824 731 32
PstI C_TGCA'G
Cuts at: 0 772 1608 3953 4587
Size: 772 836 2345 634
Fragments arranged by size:
2345 836 772 634
PvuII CAG'CTG
Cuts at: 0 348 491 4181 4337 4587
Size: 348 143 3690 156 250
Fragments arranged by size:
3690 348 250 156 143
SacI G_AGCT'C
Cuts at: 0 3402 4587
Size: 3402 1185
SfcI C'TryA_G
Cuts at: 0 768 834 1604 1928 3949 4176 4587
Size: 768 66 770 324 2021 227 411
Fragments arranged by size:
2021 770 768 411 324 227 66
SmlI C'TyrA_G
Cuts at: 0 1312 2349 4587
Size: 1312 1037 2238
Fragments arranged by size:
2238 1312 1037
SnaBI TAC'GTA
Cuts at: 0 1681 3204 4587
Size: 1681 1523 1383
Fragments arranged by size:
1681 1523 1383
SphI G_CATG'C
Cuts at: 0 3852 4587
Size: 3852 735
SspI AAT'ATT
Cuts at: 0 2742 4587
Size: 2742 1845
StuI AGG'CCT
Cuts at: 0 681 4587
Size: 681 3906
StyI C'CwwG_G
Cuts at: 0 427 1032 1235 2309 3347 3527 3740 4026
Size: 427 605 203 1074 1038 180 213 286
Cuts at: 4026 4125 4200 4587
Size: 99 75 387
Fragments arranged by size:
1074 1038 605 427 387 286 213 203
180 99 75
SwaI ATTT'AAAT
Cuts at: 0 185 4587
Size: 185 4402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2607 3360 4442 4587
Size: 2607 753 1082 145
Fragments arranged by size:
2607 1082 753 145
TatI w'GTAC_w
Cuts at: 0 695 1598 2191 4587
Size: 695 903 593 2396
Fragments arranged by size:
2396 903 695 593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 876 908 2046 2078 3335 3367 4587
Size: 876 32 1138 32 1257 32 1220
Fragments arranged by size:
1257 1220 1138 876 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2109 3690 4587
Size: 2109 1581 897
Fragments arranged by size:
2109 1581 897
XmnI GAAnn'nnTTC
Cuts at: 0 436 1760 4587
Size: 436 1324 2827
Fragments arranged by size:
2827 1324 436
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AlfI AloI
AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI
NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuII SacI SfcI SmlI
SnaBI SphI SspI StuI StyI SwaI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AleI ApaLI AscI
AsiSI AvrII BamHI BbeI BclI BlpI BmgBI BsaBI
BsaHI BsiEI BsiWI BspHI BsrBI BsrDI BssHII BssSI
Bsu36I CspCI EagI EcoRV FseI FspAI HpaI KasI
KpnI MluI NaeI NarI NgoMIV NotI NruI PacI
PmeI PmlI PshAI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SrfI Tth111I XbaI XhoI XmaI
ZraI