Restriction map for
pFN21AB0007
(Linear) (Six-base) MAPSORT of: KIBB0007.seq Check: 3865 from: 1 to: 6816
KIBB0007.orf
With 182 enzymes: SgfI *
July 15, 2010 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 812 1247 6816
Size: 812 435 5569
Fragments arranged by size:
5569 812 435
AccI GT'mk_AC
Cuts at: 0 1159 4409 4607 6816
Size: 1159 3250 198 2209
Fragments arranged by size:
3250 2209 1159 198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1013 1069 1184 1195 1363 1471 2181
Size: 412 601 56 115 11 168 108 710
Cuts at: 2181 2452 3082 3100 3265 4577 4780 5483 6199
Size: 271 630 18 165 1312 203 703 716
Cuts at: 6199 6286 6816
Size: 87 530
Fragments arranged by size:
1312 716 710 703 630 601 530 412
271 203 168 165 115 108 87 56
18 11
AfeI AGC'GCT
Cuts at: 0 6376 6816
Size: 6376 440
AflII C'TTAA_G
Cuts at: 0 2125 3048 6816
Size: 2125 923 3768
Fragments arranged by size:
3768 2125 923
AflIII A'CryG_T
Cuts at: 0 555 2345 6656 6816
Size: 555 1790 4311 160
Fragments arranged by size:
4311 1790 555 160
AhdI GACnn_n'nnGTC
Cuts at: 0 308 2088 5777 6816
Size: 308 1780 3689 1039
Fragments arranged by size:
3689 1780 1039 308
AleI CACnn'nnGTG
Cuts at: 0 5749 6816
Size: 5749 1067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3002 4478 6816
Size: 3002 1476 2338
Fragments arranged by size:
3002 2338 1476
AlwNI CAG_nnn'CTG
Cuts at: 0 219 1239 1863 2256 3183 3696 3759 4519
Size: 219 1020 624 393 927 513 63 760
Cuts at: 4519 5095 6207 6492 6816
Size: 576 1112 285 324
Fragments arranged by size:
1112 1020 927 760 624 576 513 393
324 285 219 63
ApaI G_GGCC'C
Cuts at: 0 1492 6816
Size: 1492 5324
ApoI r'AATT_y
Cuts at: 0 2746 3166 3779 4542 6003 6765 6816
Size: 2746 420 613 763 1461 762 51
Fragments arranged by size:
2746 1461 763 762 613 420 51
AseI AT'TA_AT
Cuts at: 0 4318 6816
Size: 4318 2498
AvaI C'yCGr_G
Cuts at: 0 476 1491 3131 4424 4652 4697 6693 6816
Size: 476 1015 1640 1293 228 45 1996 123
Fragments arranged by size:
1996 1640 1293 1015 476 228 123 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2518 2551 6816
Size: 2518 33 4265
Fragments arranged by size:
4265 2518 33
BamHI G'GATC_C
Cuts at: 0 4722 5109 6816
Size: 4722 387 1707
Fragments arranged by size:
4722 1707 387
BanI G'GyrC_C
Cuts at: 0 106 191 3544 5905 6816
Size: 106 85 3353 2361 911
Fragments arranged by size:
3353 2361 911 106 85
BanII G_rGCy'C
Cuts at: 0 260 453 977 1184 1395 1492 2888 3122
Size: 260 193 524 207 211 97 1396 234
Cuts at: 3122 5525 6055 6676 6816
Size: 2403 530 621 140
Fragments arranged by size:
2403 1396 621 530 524 260 234 211
207 193 140 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 1738 2084 2348 2491 3392 3811 5521 6653
Size: 1738 346 264 143 901 419 1710 1132
Cuts at: 6653 6816
Size: 163
Fragments arranged by size:
1738 1710 1132 901 419 346 264 163
143
BbvCI CC'TCA_GC
Cuts at: 0 2159 4114 5857 6816
Size: 2159 1955 1743 959
Fragments arranged by size:
2159 1955 1743 959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 752 2005 2039 6816
Size: 718 34 1253 34 4777
Fragments arranged by size:
4777 1253 718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1456 2546 6816
Size: 1456 1090 4270
Fragments arranged by size:
4270 1456 1090
BclI T'GATC_A
Cuts at: 0 140 371 1529 5835 6816
Size: 140 231 1158 4306 981
Fragments arranged by size:
4306 1158 981 231 140
BfrBI ATG'CAT
Cuts at: 0 5501 6816
Size: 5501 1315
BglI GCCn_nnn'nGGC
Cuts at: 0 665 1496 1600 6816
Size: 665 831 104 5216
Fragments arranged by size:
5216 831 665 104
BglII A'GATC_T
Cuts at: 0 3025 3075 6816
Size: 3025 50 3741
Fragments arranged by size:
3741 3025 50
BlpI GC'TnA_GC
Cuts at: 0 3257 3278 6816
Size: 3257 21 3538
Fragments arranged by size:
3538 3257 21
Bme1580I G_kGCm'C
Cuts at: 0 109 1492 2316 6815 6816
Size: 109 1383 824 4499 1
Fragments arranged by size:
4499 1383 824 109 1
BmgBI CAC'GTC
Cuts at: 0 836 6816
Size: 836 5980
BmrI ACTGGGnnnn_n'
Cuts at: 0 3204 5222 5333 6605 6816
Size: 3204 2018 111 1272 211
Fragments arranged by size:
3204 2018 1272 211 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 274 951 5433 6111 6816
Size: 274 677 4482 678 705
Fragments arranged by size:
4482 705 678 677 274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 1168 1282 2305 2662 2872 3050 3256
Size: 912 256 114 1023 357 210 178 206
Cuts at: 3256 3688 3718 3781 3973 4280 4369 4948 5089
Size: 432 30 63 192 307 89 579 141
Cuts at: 5089 6106 6175 6403 6816
Size: 1017 69 228 413
Fragments arranged by size:
1023 1017 912 579 432 413 357 307
256 228 210 206 192 178 141 114
89 69 63 30
Bpu10I CC'TnA_GC
Cuts at: 0 2159 4114 5857 6161 6816
Size: 2159 1955 1743 304 655
Fragments arranged by size:
2159 1955 1743 655 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 5145 6816
Size: 1768 3377 1671
Fragments arranged by size:
3377 1768 1671
BsaI GGTCTCn'nnnn_
Cuts at: 0 4051 4881 4993 6816
Size: 4051 830 112 1823
Fragments arranged by size:
4051 1823 830 112
BsaAI yAC'GTr
Cuts at: 0 1482 4752 6816
Size: 1482 3270 2064
Fragments arranged by size:
3270 2064 1482
BsaBI GATnn'nnATC
Cuts at: 0 1109 2681 4385 4676 6816
Size: 1109 1572 1704 291 2140
Fragments arranged by size:
2140 1704 1572 1109 291
BsaHI Gr'CG_yC
Cuts at: 0 6755 6816
Size: 6755 61
BsaWI w'CCGG_w
Cuts at: 0 4561 6455 6706 6816
Size: 4561 1894 251 110
Fragments arranged by size:
4561 1894 251 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 745 775 835 865 868 898
Size: 1 30 714 30 60 30 3 30
Cuts at: 898 3487 3517 3961 3991 5869 5899 6211 6241
Size: 2589 30 444 30 1878 30 312 30
Cuts at: 6241 6376 6395 6406 6425 6816
Size: 135 19 11 19 391
Fragments arranged by size:
2589 1878 714 444 391 312 135 60
30 30 30 30 30 30 30 30
19 19 11 3 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 772 883 886 889 1529 2392 3130
Size: 769 3 111 3 3 640 863 738
Cuts at: 3130 3253 3841 6816
Size: 123 588 2975
Fragments arranged by size:
2975 863 769 738 640 588 123 111
3 3 3
BseYI C'CCAG_C
Cuts at: 0 536 1889 2504 6643 6816
Size: 536 1353 615 4139 173
Fragments arranged by size:
4139 1353 615 536 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1324 2155 3960 4265 4774 4791 5581
Size: 286 1038 831 1805 305 509 17 790
Cuts at: 5581 6153 6816
Size: 572 663
Fragments arranged by size:
1805 1038 831 790 663 572 509 305
286 17
BsiHKAI G_wGCw'C
Cuts at: 0 260 977 1395 2042 2888 3122 6816
Size: 260 717 418 647 846 234 3694
Fragments arranged by size:
3694 846 717 647 418 260 234
BsmI GAATG_Cn'
Cuts at: 0 4571 6816
Size: 4571 2245
Bsp1286I G_dGCh'C
Cuts at: 0 109 260 453 977 1184 1395 1492 2042
Size: 109 151 193 524 207 211 97 550
Cuts at: 2042 2316 2888 3122 5525 6055 6676 6815 6816
Size: 274 572 234 2403 530 621 139 1
Fragments arranged by size:
2403 621 572 550 530 524 274 234
211 207 193 151 139 109 97 1
BspEI T'CCGG_A
Cuts at: 0 6455 6706 6816
Size: 6455 251 110
Fragments arranged by size:
6455 251 110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 731 779 812 1247 1942 3191 6535
Size: 83 648 48 33 435 695 1249 3344
Cuts at: 6535 6816
Size: 281
Fragments arranged by size:
3344 1249 695 648 435 281 83 48
33
BsrBI CCG'CTC
Cuts at: 0 6117 6680 6692 6816
Size: 6117 563 12 124
Fragments arranged by size:
6117 563 124 12
BsrDI GCAATG_nn'
Cuts at: 0 3445 4139 6056 6816
Size: 3445 694 1917 760
Fragments arranged by size:
3445 1917 760 694
BsrFI r'CCGG_y
Cuts at: 0 175 6816
Size: 175 6641
BsrGI T'GTAC_A
Cuts at: 0 1335 6816
Size: 1335 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1239 6816
Size: 1239 5577
BstEII G'GTnAC_C
Cuts at: 0 87 5129 6816
Size: 87 5042 1687
Fragments arranged by size:
5042 1687 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5751 6816
Size: 5751 1065
BstYI r'GATC_y
Cuts at: 0 2510 3025 3075 4249 4722 5109 5421 6697
Size: 2510 515 50 1174 473 387 312 1276
Cuts at: 6697 6709 6816
Size: 12 107
Fragments arranged by size:
2510 1276 1174 515 473 387 312 107
50 12
Bsu36I CC'TnA_GG
Cuts at: 0 2907 4277 5584 6816
Size: 2907 1370 1307 1232
Fragments arranged by size:
2907 1370 1307 1232
BtgI C'CryG_G
Cuts at: 0 25 1556 5289 6194 6578 6816
Size: 25 1531 3733 905 384 238
Fragments arranged by size:
3733 1531 905 384 238 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 897 5951 6816
Size: 897 5054 865
Fragments arranged by size:
5054 897 865
BtsI GCAGTG_nn'
Cuts at: 0 1075 2040 2314 5931 6344 6735 6816
Size: 1075 965 274 3617 413 391 81
Fragments arranged by size:
3617 1075 965 413 391 274 81
ClaI AT'CG_AT
Cuts at: 0 4680 6816
Size: 4680 2136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 486 521 6816
Size: 486 35 6295
Fragments arranged by size:
6295 486 35
DraIII CAC_nnn'GTG
Cuts at: 0 1097 6816
Size: 1097 5719
DrdI GACnn_nn'nnGTC
Cuts at: 0 1006 6816
Size: 1006 5810
EaeI y'GGCC_r
Cuts at: 0 1553 2474 3303 6254 6369 6474 6816
Size: 1553 921 829 2951 115 105 342
Fragments arranged by size:
2951 1553 921 829 342 115 105
EarI CTCTTCn'nnn_
Cuts at: 0 1142 1627 3058 3107 3241 3547 3926 4810
Size: 1142 485 1431 49 134 306 379 884
Cuts at: 4810 6816
Size: 2006
Fragments arranged by size:
2006 1431 1142 884 485 379 306 134
49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 613 6816
Size: 613 6203
EcoICRI GAG'CTC
Cuts at: 0 258 975 1393 2886 3120 6816
Size: 258 717 418 1493 234 3696
Fragments arranged by size:
3696 1493 717 418 258 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 912 1013 1069 1168 1184 1195 1282
Size: 412 500 101 56 99 16 11 87
Cuts at: 1282 1363 1471 2181 2305 2452 2662 2872 3050
Size: 81 108 710 124 147 210 210 178
Cuts at: 3050 3082 3100 3256 3265 3688 3718 3781 3973
Size: 32 18 156 9 423 30 63 192
Cuts at: 3973 4280 4369 4577 4780 4948 5089 5483 6106
Size: 307 89 208 203 168 141 394 623
Cuts at: 6106 6175 6199 6286 6403 6816
Size: 69 24 87 117 413
Fragments arranged by size:
710 623 500 423 413 412 394 307
210 210 208 203 192 178 168 156
147 141 124 117 108 101 99 89
87 87 81 69 63 56 32 30
24 18 16 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 789 5231 6816
Size: 789 4442 1585
Fragments arranged by size:
4442 1585 789
EcoO109I rG'GnC_Cy
Cuts at: 0 2637 4046 4428 5658 5811 6722 6734 6816
Size: 2637 1409 382 1230 153 911 12 82
Fragments arranged by size:
2637 1409 1230 911 382 153 82 12
EcoRI G'AATT_C
Cuts at: 0 6765 6816
Size: 6765 51
EcoRV GAT'ATC
Cuts at: 0 4006 6591 6816
Size: 4006 2585 225
Fragments arranged by size:
4006 2585 225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2203 3241 5488 6816
Size: 2203 1038 2247 1328
Fragments arranged by size:
2247 2203 1328 1038
HaeII r_GCGC'y
Cuts at: 0 3759 4038 6378 6816
Size: 3759 279 2340 438
Fragments arranged by size:
3759 2340 438 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 139 171 294 326 619 651
Size: 21 32 86 32 123 32 293 32
Cuts at: 651 783 815 1141 1173 1265 1297 1521 1553
Size: 132 32 326 32 92 32 224 32
Cuts at: 1553 2074 2106 2502 2534 2655 2687 3127 3159
Size: 521 32 396 32 121 32 440 32
Cuts at: 3159 3256 3288 3961 3993 5763 5795 5869 5901
Size: 97 32 673 32 1770 32 74 32
Cuts at: 5901 6211 6243 6816
Size: 310 32 573
Fragments arranged by size:
1770 673 573 521 440 396 326 310
293 224 132 123 121 97 92 86
74 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 21
HincII GTy'rAC
Cuts at: 0 952 1503 1947 4086 4410 5367 5610 5820
Size: 952 551 444 2139 324 957 243 210
Cuts at: 5820 6129 6414 6816
Size: 309 285 402
Fragments arranged by size:
2139 957 952 551 444 402 324 309
285 243 210
HindIII A'AGCT_T
Cuts at: 0 2414 3223 5014 6747 6816
Size: 2414 809 1791 1733 69
Fragments arranged by size:
2414 1791 1733 809 69
MfeI C'AATT_G
Cuts at: 0 2153 2659 6816
Size: 2153 506 4157
Fragments arranged by size:
4157 2153 506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 731 1057 2783 5076 6816
Size: 260 471 326 1726 2293 1740
Fragments arranged by size:
2293 1740 1726 471 326 260
MscI TGG'CCA
Cuts at: 0 2476 6256 6371 6816
Size: 2476 3780 115 445
Fragments arranged by size:
3780 2476 445 115
MslI CAynn'nnrTG
Cuts at: 0 943 1627 2107 2767 4339 4391 5749 6816
Size: 943 684 480 660 1572 52 1358 1067
Fragments arranged by size:
1572 1358 1067 943 684 660 480 52
MspA1I CmG'CkG
Cuts at: 0 27 95 702 813 1341 2423 2586 2597
Size: 27 68 607 111 528 1082 163 11
Cuts at: 2597 2892 4155 4758 6816
Size: 295 1263 603 2058
Fragments arranged by size:
2058 1263 1082 607 603 528 295 163
111 68 27 11
NcoI C'CATG_G
Cuts at: 0 6578 6816
Size: 6578 238
NdeI CA'TA_TG
Cuts at: 0 4041 6816
Size: 4041 2775
NsiI A_TGCA'T
Cuts at: 0 5503 6816
Size: 5503 1313
NspI r_CATG'y
Cuts at: 0 559 1227 1509 2349 4827 6660 6816
Size: 559 668 282 840 2478 1833 156
Fragments arranged by size:
2478 1833 840 668 559 282 156
PasI CC'CwG_GG
Cuts at: 0 6231 6816
Size: 6231 585
PciI A'CATG_T
Cuts at: 0 555 2345 6656 6816
Size: 555 1790 4311 160
Fragments arranged by size:
4311 1790 555 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 1863 4789 6252 6816
Size: 1863 2926 1463 564
Fragments arranged by size:
2926 1863 1463 564
PfoI T'CCnGG_A
Cuts at: 0 212 1184 3170 3665 6380 6736 6816
Size: 212 972 1986 495 2715 356 80
Fragments arranged by size:
2715 1986 972 495 356 212 80
PpuMI rG'GwC_Cy
Cuts at: 0 2637 4046 4428 6722 6734 6816
Size: 2637 1409 382 2294 12 82
Fragments arranged by size:
2637 2294 1409 382 82 12
PshAI GACnn'nnGTC
Cuts at: 0 1864 6816
Size: 1864 4952
PsiI TTA'TAA
Cuts at: 0 458 6816
Size: 458 6358
PspOMI G'GGCC_C
Cuts at: 0 1488 6816
Size: 1488 5328
PspXI vC'TCGA_Gb
Cuts at: 0 476 6816
Size: 476 6340
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1603 1635 6816
Size: 1603 32 5181
Fragments arranged by size:
5181 1603 32
PstI C_TGCA'G
Cuts at: 0 227 818 1064 1235 2018 2991 3188 3428
Size: 227 591 246 171 783 973 197 240
Cuts at: 3428 3536 3692 5199 6816
Size: 108 156 1507 1617
Fragments arranged by size:
1617 1507 973 783 591 246 240 227
197 171 156 108
PvuII CAG'CTG
Cuts at: 0 702 813 1341 2586 2892 4155 4758 6816
Size: 702 111 528 1245 306 1263 603 2058
Fragments arranged by size:
2058 1263 1245 702 603 528 306 111
SacI G_AGCT'C
Cuts at: 0 260 977 1395 2888 3122 6816
Size: 260 717 418 1493 234 3694
Fragments arranged by size:
3694 1493 717 418 260 234
SacII CC_GC'GG
Cuts at: 0 28 6816
Size: 28 6788
SalI G'TCGA_C
Cuts at: 0 4408 6816
Size: 4408 2408
SanDI GG'GwC_CC
Cuts at: 0 4046 6722 6734 6816
Size: 4046 2676 12 82
Fragments arranged by size:
4046 2676 82 12
SapI GCTCTTCn'nnn_
Cuts at: 0 1627 6816
Size: 1627 5189
ScaI AGT'ACT
Cuts at: 0 1585 2215 6816
Size: 1585 630 4601
Fragments arranged by size:
4601 1585 630
SexAI A'CCwGG_T
Cuts at: 0 5055 6816
Size: 5055 1761
SfcI C'TryA_G
Cuts at: 0 223 814 1060 1231 1362 2014 2494 2987
Size: 223 591 246 171 131 652 480 493
Cuts at: 2987 3184 3424 3532 3688 4364 5195 6816
Size: 197 240 108 156 676 831 1621
Fragments arranged by size:
1621 831 676 652 591 493 480 246
240 223 197 171 156 131 108
SmaI CCC'GGG
Cuts at: 0 1493 4654 6816
Size: 1493 3161 2162
Fragments arranged by size:
3161 2162 1493
SmlI C'TyrA_G
Cuts at: 0 476 1747 2125 3048 5124 6816
Size: 476 1271 378 923 2076 1692
Fragments arranged by size:
2076 1692 1271 923 476 378
SspI AAT'ATT
Cuts at: 0 3814 6816
Size: 3814 3002
StuI AGG'CCT
Cuts at: 0 931 1045 5647 6166 6816
Size: 931 114 4602 519 650
Fragments arranged by size:
4602 931 650 519 114
StyI C'CwwG_G
Cuts at: 0 497 1782 3456 4172 4646 4833 6578 6632
Size: 497 1285 1674 716 474 187 1745 54
Cuts at: 6632 6816
Size: 184
Fragments arranged by size:
1745 1674 1285 716 497 474 187 184
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 6203 6816
Size: 6203 613
TatI w'GTAC_w
Cuts at: 0 119 1335 1583 2213 2838 3616 4809 6816
Size: 119 1216 248 630 625 778 1193 2007
Fragments arranged by size:
2007 1216 1193 778 630 625 248 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 835 867 2658 2690 6816
Size: 835 32 1791 32 4126
Fragments arranged by size:
4126 1791 835 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3975 6223 6816
Size: 3975 2248 593
Fragments arranged by size:
3975 2248 593
XbaI T'CTAG_A
Cuts at: 0 3288 3729 6816
Size: 3288 441 3087
Fragments arranged by size:
3288 3087 441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 570 5092 5754 5916 6816
Size: 348 222 4522 662 162 900
Fragments arranged by size:
4522 900 662 348 222 162
XhoI C'TCGA_G
Cuts at: 0 476 6816
Size: 476 6340
XmaI C'CCGG_G
Cuts at: 0 1491 4652 6816
Size: 1491 3161 2164
Fragments arranged by size:
3161 2164 1491
XmnI GAAnn'nnTTC
Cuts at: 0 3166 6283 6463 6816
Size: 3166 3117 180 353
Fragments arranged by size:
3166 3117 353 180
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PasI
PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuII SacI SacII SalI SanDI SapI
ScaI SexAI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AloI ApaLI AscI AsiSI
AvrII BbeI BmtI BsiEI BsiWI BsmBI BspHI BssHII
BssSI BstBI BstZ17I DraI EagI FseI FspI FspAI
HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpiI PvuI RsrII
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI ZraI