Restriction map for
pFN21AA1903
(Linear) (Six-base) MAPSORT of: KIAA1903.seq Check: 7516 from: 1 to: 3396
KIAA1903.orf
With 182 enzymes: SgfI *
July 15, 2010 18:28 ..
AatII G_ACGT'C
Cuts at: 0 1223 3396
Size: 1223 2173
AclI AA'CG_TT
Cuts at: 0 1298 3396
Size: 1298 2098
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1618 1868 2378 2483 2512 2765 3396
Size: 89 1529 250 510 105 29 253 631
Fragments arranged by size:
1529 631 510 253 250 105 89 29
AflIII A'CryG_T
Cuts at: 0 797 1015 1643 2799 3396
Size: 797 218 628 1156 597
Fragments arranged by size:
1156 797 628 597 218
AhdI GACnn_n'nnGTC
Cuts at: 0 2512 3396
Size: 2512 884
AlwNI CAG_nnn'CTG
Cuts at: 0 979 1546 3396
Size: 979 567 1850
Fragments arranged by size:
1850 979 567
ApoI r'AATT_y
Cuts at: 0 29 687 746 1328 1372 2479 2926 3396
Size: 29 658 59 582 44 1107 447 470
Fragments arranged by size:
1107 658 582 470 447 59 44 29
AseI AT'TA_AT
Cuts at: 0 1750 3396
Size: 1750 1646
BamHI G'GATC_C
Cuts at: 0 2788 3396
Size: 2788 608
BanI G'GyrC_C
Cuts at: 0 2017 3396
Size: 2017 1379
BanII G_rGCy'C
Cuts at: 0 732 3396
Size: 732 2664
BbsI GAAGACnn'nnnn_
Cuts at: 0 985 1769 3396
Size: 985 784 1627
Fragments arranged by size:
1627 985 784
BciVI GTATCCnnnnn_n'
Cuts at: 0 1872 3396
Size: 1872 1524
BclI T'GATC_A
Cuts at: 0 2859 3396
Size: 2859 537
BfrBI ATG'CAT
Cuts at: 0 1994 3396
Size: 1994 1402
BglI GCCn_nnn'nGGC
Cuts at: 0 2840 3396
Size: 2840 556
BglII A'GATC_T
Cuts at: 0 1486 3396
Size: 1486 1910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1001 3396
Size: 26 975 2395
Fragments arranged by size:
2395 975 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1866 3396
Size: 1866 1530
BsaBI GATnn'nnATC
Cuts at: 0 2858 3396
Size: 2858 538
BsaHI Gr'CG_yC
Cuts at: 0 1220 3396
Size: 1220 2176
BsaWI w'CCGG_w
Cuts at: 0 2168 3396
Size: 2168 1228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 102 132 429 459 3396
Size: 102 30 297 30 2937
Fragments arranged by size:
2937 297 102 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 1085 1736 1739 3095 3396
Size: 141 944 651 3 1356 301
Fragments arranged by size:
1356 944 651 301 141 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 3396
Size: 625 2771
BsiHKAI G_wGCw'C
Cuts at: 0 732 1271 1409 2419 3396
Size: 732 539 138 1010 977
Fragments arranged by size:
1010 977 732 539 138
BsmI GAATG_Cn'
Cuts at: 0 98 2611 2755 3396
Size: 98 2513 144 641
Fragments arranged by size:
2513 641 144 98
Bsp1286I G_dGCh'C
Cuts at: 0 732 1271 1409 2419 3396
Size: 732 539 138 1010 977
Fragments arranged by size:
1010 977 732 539 138
BsrBI CCG'CTC
Cuts at: 0 1260 3396
Size: 1260 2136
BssSI C'ACGA_G
Cuts at: 0 1526 3396
Size: 1526 1870
BstBI TT'CG_AA
Cuts at: 0 3379 3396
Size: 3379 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1397 3396
Size: 1397 1999
BstYI r'GATC_y
Cuts at: 0 1486 2575 2788 3396
Size: 1486 1089 213 608
Fragments arranged by size:
1486 1089 608 213
BtgI C'CryG_G
Cuts at: 0 74 2084 3396
Size: 74 2010 1312
Fragments arranged by size:
2010 1312 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2156 3396
Size: 2156 1240
BtsI GCAGTG_nn'
Cuts at: 0 637 2402 3396
Size: 637 1765 994
Fragments arranged by size:
1765 994 637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1639 1674 3396
Size: 1639 35 1722
Fragments arranged by size:
1722 1639 35
EaeI y'GGCC_r
Cuts at: 0 1922 3396
Size: 1922 1474
EarI CTCTTCn'nnn_
Cuts at: 0 1594 2531 2687 3353 3396
Size: 1594 937 156 666 43
Fragments arranged by size:
1594 937 666 156 43
EcoICRI GAG'CTC
Cuts at: 0 730 3396
Size: 730 2666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 89 1001 1618 1868 2378 2483 2512
Size: 26 63 912 617 250 510 105 29
Cuts at: 2512 2765 3396
Size: 253 631
Fragments arranged by size:
912 631 617 510 253 250 105 63
29 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2194 3396
Size: 2194 1202
EcoRV GAT'ATC
Cuts at: 0 273 1351 3396
Size: 273 1078 2045
Fragments arranged by size:
2045 1078 273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2542 3396
Size: 2542 854
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 1720 1752 1989 2021 2498 2530
Size: 533 32 1155 32 237 32 477 32
Cuts at: 2530 3396
Size: 866
Fragments arranged by size:
1155 866 533 477 237 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2166 3396
Size: 2166 1230
HindIII A'AGCT_T
Cuts at: 0 1087 1940 3396
Size: 1087 853 1456
Fragments arranged by size:
1456 1087 853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 688 3088 3286 3396
Size: 389 299 2400 198 110
Fragments arranged by size:
2400 389 299 198 110
MscI TGG'CCA
Cuts at: 0 1924 3396
Size: 1924 1472
MslI CAynn'nnrTG
Cuts at: 0 1244 3396
Size: 1244 2152
MspA1I CmG'CkG
Cuts at: 0 2014 3396
Size: 2014 1382
NcoI C'CATG_G
Cuts at: 0 74 2084 3396
Size: 74 2010 1312
Fragments arranged by size:
2010 1312 74
NsiI A_TGCA'T
Cuts at: 0 1996 3396
Size: 1996 1400
NspI r_CATG'y
Cuts at: 0 1019 1647 2803 3121 3135 3396
Size: 1019 628 1156 318 14 261
Fragments arranged by size:
1156 1019 628 318 261 14
PciI A'CATG_T
Cuts at: 0 1015 1643 2799 3396
Size: 1015 628 1156 597
Fragments arranged by size:
1156 1015 628 597
PfoI T'CCnGG_A
Cuts at: 0 3014 3396
Size: 3014 382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2192 2224 3396
Size: 2192 32 1172
Fragments arranged by size:
2192 1172 32
PsiI TTA'TAA
Cuts at: 0 2288 3396
Size: 2288 1108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2138 2170 3396
Size: 2138 32 1226
Fragments arranged by size:
2138 1226 32
SacI G_AGCT'C
Cuts at: 0 732 3396
Size: 732 2664
ScaI AGT'ACT
Cuts at: 0 365 867 3396
Size: 365 502 2529
Fragments arranged by size:
2529 502 365
SexAI A'CCwGG_T
Cuts at: 0 2613 2697 3396
Size: 2613 84 699
Fragments arranged by size:
2613 699 84
SfcI C'TryA_G
Cuts at: 0 698 1153 1631 2726 3396
Size: 698 455 478 1095 670
Fragments arranged by size:
1095 698 670 478 455
SmlI C'TyrA_G
Cuts at: 0 1881 3396
Size: 1881 1515
SphI G_CATG'C
Cuts at: 0 3135 3396
Size: 3135 261
SspI AAT'ATT
Cuts at: 0 341 1080 1106 2436 3396
Size: 341 739 26 1330 960
Fragments arranged by size:
1330 960 739 341 26
StyI C'CwwG_G
Cuts at: 0 74 552 2084 3396
Size: 74 478 1532 1312
Fragments arranged by size:
1532 1312 478 74
TatI w'GTAC_w
Cuts at: 0 363 865 1026 2971 3396
Size: 363 502 161 1945 425
Fragments arranged by size:
1945 502 425 363 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 102 134 959 991 3144 3176 3396
Size: 102 32 825 32 2153 32 220
Fragments arranged by size:
2153 825 220 102 32 32 32
XbaI T'CTAG_A
Cuts at: 0 564 1969 3396
Size: 564 1405 1427
Fragments arranged by size:
1427 1405 564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2394 3396
Size: 2394 1002
XmnI GAAnn'nnTTC
Cuts at: 0 184 836 1080 2525 3396
Size: 184 652 244 1445 871
Fragments arranged by size:
1445 871 652 244 184
ZraI GAC'GTC
Cuts at: 0 1221 3396
Size: 1221 2175
Enzymes that do cut:
AatII AclI AcuI AflIII AhdI AlwNI ApoI AseI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglI
BglII BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrBI BssSI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoRV FalI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NsiI NspI PciI PfoI
PpiI PsiI PsrI SacI ScaI SexAI SfcI SmlI
SphI SspI StyI TatI TstI XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI
BstEII BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI
EciI EcoO109I EcoRI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XhoI XmaI