Restriction map for
pFN21AA1844
(Linear) (Six-base) MAPSORT of: KIAA1844.seq Check: 566 from: 1 to: 1941
KIAA1844.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1004 1054 1528 1671 1941
Size: 356 648 50 474 143 270
Fragments arranged by size:
648 474 356 270 143 50
AfeI AGC'GCT
Cuts at: 0 1549 1941
Size: 1549 392
AflII C'TTAA_G
Cuts at: 0 82 1941
Size: 82 1859
AflIII A'CryG_T
Cuts at: 0 1654 1707 1790 1941
Size: 1654 53 83 151
Fragments arranged by size:
1654 151 83 53
AleI CACnn'nnGTG
Cuts at: 0 1688 1941
Size: 1688 253
AlwNI CAG_nnn'CTG
Cuts at: 0 1177 1941
Size: 1177 764
ApoI r'AATT_y
Cuts at: 0 297 330 1635 1799 1818 1847 1926 1941
Size: 297 33 1305 164 19 29 79 15
Fragments arranged by size:
1305 297 164 79 33 29 19 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1610 1643 1941
Size: 1610 33 298
Fragments arranged by size:
1610 298 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 965 1534 1941
Size: 620 345 569 407
Fragments arranged by size:
620 569 407 345
BciVI GTATCCnnnnn_n'
Cuts at: 0 1868 1941
Size: 1868 73
BclI T'GATC_A
Cuts at: 0 117 816 1403 1941
Size: 117 699 587 538
Fragments arranged by size:
699 587 538 117
BfrBI ATG'CAT
Cuts at: 0 1609 1941
Size: 1609 332
BglII A'GATC_T
Cuts at: 0 181 1825 1941
Size: 181 1644 116
Fragments arranged by size:
1644 181 116
BlpI GC'TnA_GC
Cuts at: 0 1271 1941
Size: 1271 670
BmgBI CAC'GTC
Cuts at: 0 971 1941
Size: 971 970
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 1941
Size: 101 1840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1941
Size: 1430 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1536 1941
Size: 1247 289 405
Fragments arranged by size:
1247 405 289
BsaAI yAC'GTr
Cuts at: 0 1655 1941
Size: 1655 286
BsaHI Gr'CG_yC
Cuts at: 0 143 1941
Size: 143 1798
BsaWI w'CCGG_w
Cuts at: 0 50 1941
Size: 50 1891
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1403 1523 1941
Size: 1403 120 418
Fragments arranged by size:
1403 418 120
BseYI C'CCAG_C
Cuts at: 0 1289 1941
Size: 1289 652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 986 1655 1941
Size: 881 105 669 286
Fragments arranged by size:
881 669 286 105
BsiEI CG_ry'CG
Cuts at: 0 51 1941
Size: 51 1890
BsiHKAI G_wGCw'C
Cuts at: 0 318 1941
Size: 318 1623
BsmI GAATG_Cn'
Cuts at: 0 621 1941
Size: 621 1320
BsmBI CGTCTCn'nnnn_
Cuts at: 0 134 1256 1941
Size: 134 1122 685
Fragments arranged by size:
1122 685 134
Bsp1286I G_dGCh'C
Cuts at: 0 318 1941
Size: 318 1623
BspHI T'CATG_A
Cuts at: 0 1518 1941
Size: 1518 423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1615 1732 1941
Size: 1615 117 209
Fragments arranged by size:
1615 209 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 485 609 1941
Size: 485 124 1332
Fragments arranged by size:
1332 485 124
BstYI r'GATC_y
Cuts at: 0 150 181 1825 1941
Size: 150 31 1644 116
Fragments arranged by size:
1644 150 116 31
BtsI GCAGTG_nn'
Cuts at: 0 1192 1941
Size: 1192 749
EarI CTCTTCn'nnn_
Cuts at: 0 91 323 426 558 1941
Size: 91 232 103 132 1383
Fragments arranged by size:
1383 232 132 103 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1004 1054 1430 1528 1671 1941
Size: 356 648 50 376 98 143 270
Fragments arranged by size:
648 376 356 270 143 98 50
EcoO109I rG'GnC_Cy
Cuts at: 0 1074 1941
Size: 1074 867
EcoRI G'AATT_C
Cuts at: 0 1635 1926 1941
Size: 1635 291 15
Fragments arranged by size:
1635 291 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 386 505 1941
Size: 386 119 1436
Fragments arranged by size:
1436 386 119
HaeII r_GCGC'y
Cuts at: 0 1551 1941
Size: 1551 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 1318 1350 1395 1427 1817 1849
Size: 388 32 898 32 45 32 390 32
Cuts at: 1849 1941
Size: 92
Fragments arranged by size:
898 390 388 92 45 32 32 32
32
HincII GTy'rAC
Cuts at: 0 534 1941
Size: 534 1407
MfeI C'AATT_G
Cuts at: 0 444 1093 1941
Size: 444 649 848
Fragments arranged by size:
848 649 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1033 1941
Size: 790 243 908
Fragments arranged by size:
908 790 243
MslI CAynn'nnrTG
Cuts at: 0 125 626 1688 1941
Size: 125 501 1062 253
Fragments arranged by size:
1062 501 253 125
MspA1I CmG'CkG
Cuts at: 0 1024 1177 1941
Size: 1024 153 764
Fragments arranged by size:
1024 764 153
NdeI CA'TA_TG
Cuts at: 0 1347 1941
Size: 1347 594
NsiI A_TGCA'T
Cuts at: 0 1611 1941
Size: 1611 330
NspI r_CATG'y
Cuts at: 0 1794 1941
Size: 1794 147
PciI A'CATG_T
Cuts at: 0 1790 1941
Size: 1790 151
PflMI CCAn_nnn'nTGG
Cuts at: 0 1576 1941
Size: 1576 365
PmlI CAC'GTG
Cuts at: 0 1655 1941
Size: 1655 286
PpuMI rG'GwC_Cy
Cuts at: 0 1074 1941
Size: 1074 867
PshAI GACnn'nnGTC
Cuts at: 0 1467 1941
Size: 1467 474
PstI C_TGCA'G
Cuts at: 0 396 1124 1199 1728 1941
Size: 396 728 75 529 213
Fragments arranged by size:
728 529 396 213 75
PvuII CAG'CTG
Cuts at: 0 1177 1941
Size: 1177 764
SapI GCTCTTCn'nnn_
Cuts at: 0 323 426 1941
Size: 323 103 1515
Fragments arranged by size:
1515 323 103
ScaI AGT'ACT
Cuts at: 0 436 1410 1443 1941
Size: 436 974 33 498
Fragments arranged by size:
974 498 436 33
SexAI A'CCwGG_T
Cuts at: 0 1575 1941
Size: 1575 366
SfcI C'TryA_G
Cuts at: 0 392 693 808 1120 1195 1724 1941
Size: 392 301 115 312 75 529 217
Fragments arranged by size:
529 392 312 301 217 115 75
SmlI C'TyrA_G
Cuts at: 0 82 1262 1551 1941
Size: 82 1180 289 390
Fragments arranged by size:
1180 390 289 82
SpeI A'CTAG_T
Cuts at: 0 589 1941
Size: 589 1352
SspI AAT'ATT
Cuts at: 0 657 1941
Size: 657 1284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 894 1941
Size: 894 1047
TatI w'GTAC_w
Cuts at: 0 434 735 1408 1441 1804 1941
Size: 434 301 673 33 363 137
Fragments arranged by size:
673 434 363 301 137 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1941
Size: 753 1188
XmnI GAAnn'nnTTC
Cuts at: 0 1930 1941
Size: 1930 11
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI AlwNI ApoI BaeI
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BstAPI BstXI
BstYI BtsI EarI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII MfeI MmeI MslI MspA1I NdeI NsiI
NspI PciI PflMI PmlI PpuMI PshAI PstI PvuII
SapI ScaI SexAI SfcI SmlI SpeI SspI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BmtI BplI Bpu10I BsaI BsaBI BsaXI BsiWI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI StyI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI