Restriction map for
pFN21AA1588
(Linear) (Six-base) MAPSORT of: KIAA1588.seq Check: 7514 from: 1 to: 1785
KIAA1588.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1515 1570 1599 1683 1785
Size: 770 745 55 29 84 102
Fragments arranged by size:
770 745 102 84 55 29
AflII C'TTAA_G
Cuts at: 0 1297 1785
Size: 1297 488
AflIII A'CryG_T
Cuts at: 0 1085 1785
Size: 1085 700
AleI CACnn'nnGTG
Cuts at: 0 1013 1785
Size: 1013 772
AlwNI CAG_nnn'CTG
Cuts at: 0 761 1785
Size: 761 1024
ApaLI G'TGCA_C
Cuts at: 0 1089 1639 1785
Size: 1089 550 146
Fragments arranged by size:
1089 550 146
ApoI r'AATT_y
Cuts at: 0 64 102 1785
Size: 64 38 1683
Fragments arranged by size:
1683 64 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 274 307 1785
Size: 274 33 1478
Fragments arranged by size:
1478 274 33
BamHI G'GATC_C
Cuts at: 0 1649 1785
Size: 1649 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 1168 1279 1437 1558 1785
Size: 1168 111 158 121 227
Fragments arranged by size:
1168 227 158 121 111
BclI T'GATC_A
Cuts at: 0 924 1785
Size: 924 861
BfrBI ATG'CAT
Cuts at: 0 663 856 1785
Size: 663 193 929
Fragments arranged by size:
929 663 193
Bme1580I G_kGCm'C
Cuts at: 0 1093 1643 1785
Size: 1093 550 142
Fragments arranged by size:
1093 550 142
BmgBI CAC'GTC
Cuts at: 0 29 1785
Size: 29 1756
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 295 446 1785
Size: 140 155 151 1339
Fragments arranged by size:
1339 155 151 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 380 1768 1785
Size: 380 1388 17
Fragments arranged by size:
1388 380 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 151 280 1343 1765 1785
Size: 37 114 129 1063 422 20
Fragments arranged by size:
1063 422 129 114 37 20
BsaAI yAC'GTr
Cuts at: 0 1056 1728 1785
Size: 1056 672 57
Fragments arranged by size:
1056 672 57
BsaBI GATnn'nnATC
Cuts at: 0 1234 1785
Size: 1234 551
BsaWI w'CCGG_w
Cuts at: 0 860 985 1785
Size: 860 125 800
Fragments arranged by size:
860 800 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1570 1600 1785
Size: 1570 30 185
Fragments arranged by size:
1570 185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 352 367 1785
Size: 349 3 15 1418
Fragments arranged by size:
1418 349 15 3
BseYI C'CCAG_C
Cuts at: 0 307 1785
Size: 307 1478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 401 774 1785
Size: 141 260 373 1011
Fragments arranged by size:
1011 373 260 141
BsiEI CG_ry'CG
Cuts at: 0 948 1785
Size: 948 837
BsiHKAI G_wGCw'C
Cuts at: 0 1055 1093 1643 1785
Size: 1055 38 550 142
Fragments arranged by size:
1055 550 142 38
BsiWI C'GTAC_G
Cuts at: 0 1586 1785
Size: 1586 199
BsmI GAATG_Cn'
Cuts at: 0 856 1186 1429 1597 1681 1785
Size: 856 330 243 168 84 104
Fragments arranged by size:
856 330 243 168 104 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1407 1785
Size: 1407 378
Bsp1286I G_dGCh'C
Cuts at: 0 1055 1093 1643 1785
Size: 1055 38 550 142
Fragments arranged by size:
1055 550 142 38
BspEI T'CCGG_A
Cuts at: 0 860 1785
Size: 860 925
BsrFI r'CCGG_y
Cuts at: 0 475 1785
Size: 475 1310
BsrGI T'GTAC_A
Cuts at: 0 242 1785
Size: 242 1543
BssSI C'ACGA_G
Cuts at: 0 696 1204 1785
Size: 696 508 581
Fragments arranged by size:
696 581 508
BstAPI GCAn_nnn'nTGC
Cuts at: 0 761 1097 1785
Size: 761 336 688
Fragments arranged by size:
761 688 336
BstBI TT'CG_AA
Cuts at: 0 515 1785
Size: 515 1270
BstYI r'GATC_y
Cuts at: 0 1649 1785
Size: 1649 136
Bsu36I CC'TnA_GG
Cuts at: 0 793 1717 1785
Size: 793 924 68
Fragments arranged by size:
924 793 68
BtgI C'CryG_G
Cuts at: 0 1250 1785
Size: 1250 535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 948 1785
Size: 948 837
BtsI GCAGTG_nn'
Cuts at: 0 129 1102 1270 1522 1785
Size: 129 973 168 252 263
Fragments arranged by size:
973 263 252 168 129
DraIII CAC_nnn'GTG
Cuts at: 0 148 1097 1155 1785
Size: 148 949 58 630
Fragments arranged by size:
949 630 148 58
EaeI y'GGCC_r
Cuts at: 0 945 1785
Size: 945 840
EagI C'GGCC_G
Cuts at: 0 945 1785
Size: 945 840
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1482 1662 1785
Size: 1482 180 123
Fragments arranged by size:
1482 180 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 151 280 770 1343 1515 1570 1599
Size: 37 114 129 490 573 172 55 29
Cuts at: 1599 1683 1765 1785
Size: 84 82 20
Fragments arranged by size:
573 490 172 129 114 84 82 55
37 29 20
EcoO109I rG'GnC_Cy
Cuts at: 0 575 995 1785
Size: 575 420 790
Fragments arranged by size:
790 575 420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1143 1785
Size: 1143 642
HaeII r_GCGC'y
Cuts at: 0 365 377 1785
Size: 365 12 1408
Fragments arranged by size:
1408 365 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 564 596 1192 1224 1444 1476
Size: 382 32 150 32 596 32 220 32
Cuts at: 1476 1696 1728 1785
Size: 220 32 57
Fragments arranged by size:
596 382 220 220 150 57 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1544 1785
Size: 1544 241
HindIII A'AGCT_T
Cuts at: 0 1022 1358 1785
Size: 1022 336 427
Fragments arranged by size:
1022 427 336
MluI A'CGCG_T
Cuts at: 0 1085 1785
Size: 1085 700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1652 1785
Size: 1148 504 133
Fragments arranged by size:
1148 504 133
MslI CAynn'nnrTG
Cuts at: 0 640 1013 1338 1767 1785
Size: 640 373 325 429 18
Fragments arranged by size:
640 429 373 325 18
MspA1I CmG'CkG
Cuts at: 0 1115 1785
Size: 1115 670
NsiI A_TGCA'T
Cuts at: 0 665 858 1785
Size: 665 193 927
Fragments arranged by size:
927 665 193
NspI r_CATG'y
Cuts at: 0 645 891 1563 1785
Size: 645 246 672 222
Fragments arranged by size:
672 645 246 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1155 1785
Size: 105 1050 630
Fragments arranged by size:
1050 630 105
PfoI T'CCnGG_A
Cuts at: 0 53 1210 1785
Size: 53 1157 575
Fragments arranged by size:
1157 575 53
PmlI CAC'GTG
Cuts at: 0 1056 1728 1785
Size: 1056 672 57
Fragments arranged by size:
1056 672 57
PshAI GACnn'nnGTC
Cuts at: 0 839 1785
Size: 839 946
PstI C_TGCA'G
Cuts at: 0 841 1785
Size: 841 944
SfcI C'TryA_G
Cuts at: 0 267 837 1785
Size: 267 570 948
Fragments arranged by size:
948 570 267
SmlI C'TyrA_G
Cuts at: 0 1297 1785
Size: 1297 488
StuI AGG'CCT
Cuts at: 0 912 1528 1612 1785
Size: 912 616 84 173
Fragments arranged by size:
912 616 173 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 159 1785
Size: 49 110 1626
Fragments arranged by size:
1626 110 49
TatI w'GTAC_w
Cuts at: 0 242 1785
Size: 242 1543
XmnI GAAnn'nnTTC
Cuts at: 0 772 1024 1276 1360 1444 1612 1696 1785
Size: 772 252 252 84 84 168 84 89
Fragments arranged by size:
772 252 252 168 89 84 84 84
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaLI ApoI BaeI
BamHI BbsI BclI BfrBI Bme1580I BmgBI BmrI BplI
BpmI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII MluI MmeI MslI MspA1I
NsiI NspI PflMI PfoI PmlI PshAI PstI SfcI
SmlI StuI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BanI BanII BbeI BbvCI BcgI BciVI BglI BglII
BlpI BmtI Bpu10I BpuEI BsaI BsaHI BspHI BspMI
BsrBI BsrDI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraI DrdI EarI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI