Restriction map for
pFN21AA1461
(Linear) (Six-base) MAPSORT of: KIAA1461.seq Check: 6313 from: 1 to: 4482
KIAA1461.orf
With 182 enzymes: SgfI *
February 17, 2010 09:38 ..
AccI GT'mk_AC
Cuts at: 0 590 4482
Size: 590 3892
Acc65I G'GTAC_C
Cuts at: 0 1164 2963 4482
Size: 1164 1799 1519
Fragments arranged by size:
1799 1519 1164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1021 1862 2230 2531 2816 4040 4482
Size: 812 209 841 368 301 285 1224 442
Fragments arranged by size:
1224 841 812 442 368 301 285 209
AhdI GACnn_n'nnGTC
Cuts at: 0 4401 4482
Size: 4401 81
AleI CACnn'nnGTG
Cuts at: 0 2464 4482
Size: 2464 2018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1727 3086 3313 4482
Size: 1727 1359 227 1169
Fragments arranged by size:
1727 1359 1169 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4087 4119 4482
Size: 4087 32 363
Fragments arranged by size:
4087 363 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2904 4482
Size: 2904 1578
ApaLI G'TGCA_C
Cuts at: 0 1327 2275 3860 4482
Size: 1327 948 1585 622
Fragments arranged by size:
1585 1327 948 622
ApoI r'AATT_y
Cuts at: 0 471 1410 2412 2444 3477 3640 3773 3786
Size: 471 939 1002 32 1033 163 133 13
Cuts at: 3786 3978 4482
Size: 192 504
Fragments arranged by size:
1033 1002 939 504 471 192 163 133
32 13
AseI AT'TA_AT
Cuts at: 0 1127 1573 4482
Size: 1127 446 2909
Fragments arranged by size:
2909 1127 446
AvaI C'yCGr_G
Cuts at: 0 419 2160 4249 4270 4482
Size: 419 1741 2089 21 212
Fragments arranged by size:
2089 1741 419 212 21
BanI G'GyrC_C
Cuts at: 0 1164 2554 2963 4482
Size: 1164 1390 409 1519
Fragments arranged by size:
1519 1390 1164 409
BanII G_rGCy'C
Cuts at: 0 780 3113 3492 4271 4482
Size: 780 2333 379 779 211
Fragments arranged by size:
2333 780 779 379 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 4223 4288 4482
Size: 37 4186 65 194
Fragments arranged by size:
4186 194 65 37
BbvCI CC'TCA_GC
Cuts at: 0 3472 4482
Size: 3472 1010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1900 1934 4482
Size: 1900 34 2548
Fragments arranged by size:
2548 1900 34
BclI T'GATC_A
Cuts at: 0 3456 4482
Size: 3456 1026
BfrBI ATG'CAT
Cuts at: 0 299 4482
Size: 299 4183
BglI GCCn_nnn'nGGC
Cuts at: 0 656 4482
Size: 656 3826
BglII A'GATC_T
Cuts at: 0 1691 4472 4482
Size: 1691 2781 10
Fragments arranged by size:
2781 1691 10
BlpI GC'TnA_GC
Cuts at: 0 3913 4482
Size: 3913 569
Bme1580I G_kGCm'C
Cuts at: 0 1331 2279 2557 3864 4482
Size: 1331 948 278 1307 618
Fragments arranged by size:
1331 1307 948 618 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 110 2219 4482
Size: 110 2109 2263
Fragments arranged by size:
2263 2109 110
BmtI G_CTAG'C
Cuts at: 0 1765 2369 4482
Size: 1765 604 2113
Fragments arranged by size:
2113 1765 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 389 1295 1835 2447 2809 2941 2959
Size: 254 135 906 540 612 362 132 18
Cuts at: 2959 3393 4461 4482
Size: 434 1068 21
Fragments arranged by size:
1068 906 612 540 434 362 254 135
132 21 18
Bpu10I CC'TnA_GC
Cuts at: 0 2062 3472 4482
Size: 2062 1410 1010
Fragments arranged by size:
2062 1410 1010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2163 4482
Size: 2163 2319
BsaI GGTCTCn'nnnn_
Cuts at: 0 4440 4482
Size: 4440 42
BsaBI GATnn'nnATC
Cuts at: 0 1541 4482
Size: 1541 2941
BsaHI Gr'CG_yC
Cuts at: 0 2603 4482
Size: 2603 1879
BsaWI w'CCGG_w
Cuts at: 0 4319 4482
Size: 4319 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 227 257 563 593 2780 2810 4087 4117
Size: 227 30 306 30 2187 30 1277 30
Cuts at: 4117 4482
Size: 365
Fragments arranged by size:
2187 1277 365 306 227 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 401 803 1007 2743 3958 4479 4482
Size: 401 402 204 1736 1215 521 3
Fragments arranged by size:
1736 1215 521 402 401 204 3
BseYI C'CCAG_C
Cuts at: 0 664 669 1671 2746 3009 4482
Size: 664 5 1002 1075 263 1473
Fragments arranged by size:
1473 1075 1002 664 263 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1634 4482
Size: 1634 2848
BsiHKAI G_wGCw'C
Cuts at: 0 374 780 1035 1331 2279 2687 3864 4482
Size: 374 406 255 296 948 408 1177 618
Fragments arranged by size:
1177 948 618 408 406 374 296 255
BsmI GAATG_Cn'
Cuts at: 0 1076 1281 1682 3302 4482
Size: 1076 205 401 1620 1180
Fragments arranged by size:
1620 1180 1076 401 205
Bsp1286I G_dGCh'C
Cuts at: 0 374 780 1035 1331 2279 2557 2687 3113
Size: 374 406 255 296 948 278 130 426
Cuts at: 3113 3492 3864 4271 4482
Size: 379 372 407 211
Fragments arranged by size:
948 426 407 406 379 374 372 296
278 255 211 130
BspEI T'CCGG_A
Cuts at: 0 4319 4482
Size: 4319 163
BspHI T'CATG_A
Cuts at: 0 456 1415 3459 3681 4374 4482
Size: 456 959 2044 222 693 108
Fragments arranged by size:
2044 959 693 456 222 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 167 2332 3291 4482
Size: 135 32 2165 959 1191
Fragments arranged by size:
2165 1191 959 135 32
BsrDI GCAATG_nn'
Cuts at: 0 875 983 1222 1963 4482
Size: 875 108 239 741 2519
Fragments arranged by size:
2519 875 741 239 108
BsrFI r'CCGG_y
Cuts at: 0 1825 4482
Size: 1825 2657
BsrGI T'GTAC_A
Cuts at: 0 546 852 1266 4413 4482
Size: 546 306 414 3147 69
Fragments arranged by size:
3147 546 414 306 69
BssSI C'ACGA_G
Cuts at: 0 971 4482
Size: 971 3511
BstEII G'GTnAC_C
Cuts at: 0 1472 3391 4482
Size: 1472 1919 1091
Fragments arranged by size:
1919 1472 1091
BstXI CCAn_nnnn'nTGG
Cuts at: 0 694 4009 4482
Size: 694 3315 473
Fragments arranged by size:
3315 694 473
BstYI r'GATC_y
Cuts at: 0 1065 1691 2220 4472 4482
Size: 1065 626 529 2252 10
Fragments arranged by size:
2252 1065 626 529 10
Bsu36I CC'TnA_GG
Cuts at: 0 4090 4482
Size: 4090 392
BtgI C'CryG_G
Cuts at: 0 530 642 651 3026 3513 4482
Size: 530 112 9 2375 487 969
Fragments arranged by size:
2375 969 530 487 112 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1349 4482
Size: 1349 3133
BtsI GCAGTG_nn'
Cuts at: 0 329 621 1617 1836 2277 2615 2632 3314
Size: 329 292 996 219 441 338 17 682
Cuts at: 3314 3377 3706 3917 4482
Size: 63 329 211 565
Fragments arranged by size:
996 682 565 441 338 329 329 292
219 211 63 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2720 2755 4482
Size: 2720 35 1727
Fragments arranged by size:
2720 1727 35
DraI TTT'AAA
Cuts at: 0 1685 1859 4482
Size: 1685 174 2623
Fragments arranged by size:
2623 1685 174
DraIII CAC_nnn'GTG
Cuts at: 0 3169 4432 4482
Size: 3169 1263 50
Fragments arranged by size:
3169 1263 50
EaeI y'GGCC_r
Cuts at: 0 326 2676 4482
Size: 326 2350 1806
Fragments arranged by size:
2350 1806 326
EarI CTCTTCn'nnn_
Cuts at: 0 1040 4116 4482
Size: 1040 3076 366
Fragments arranged by size:
3076 1040 366
EcoICRI GAG'CTC
Cuts at: 0 778 4482
Size: 778 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 389 812 1021 1295 1835 1862 2230
Size: 254 135 423 209 274 540 27 368
Cuts at: 2230 2447 2531 2809 2816 2941 2959 3393 4040
Size: 217 84 278 7 125 18 434 647
Cuts at: 4040 4461 4482
Size: 421 21
Fragments arranged by size:
647 540 434 423 421 368 278 274
254 217 209 135 125 84 27 21
18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2261 2834 4482
Size: 2261 573 1648
Fragments arranged by size:
2261 1648 573
EcoO109I rG'GnC_Cy
Cuts at: 0 1432 1493 2171 4482
Size: 1432 61 678 2311
Fragments arranged by size:
2311 1432 678 61
EcoRI G'AATT_C
Cuts at: 0 3477 4482
Size: 3477 1005
EcoRV GAT'ATC
Cuts at: 0 1560 4482
Size: 1560 2922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 204 1087 4482
Size: 61 143 883 3395
Fragments arranged by size:
3395 883 143 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2373 2405 2839 2865 2871 2897 2913 2945
Size: 2373 32 434 26 6 26 16 32
Cuts at: 2945 3934 3966 4093 4125 4387 4419 4482
Size: 989 32 127 32 262 32 63
Fragments arranged by size:
2373 989 434 262 127 63 32 32
32 32 32 26 26 16 6
HincII GTy'rAC
Cuts at: 0 1484 3205 3324 4240 4482
Size: 1484 1721 119 916 242
Fragments arranged by size:
1721 1484 916 242 119
KpnI G_GTAC'C
Cuts at: 0 1168 2967 4482
Size: 1168 1799 1515
Fragments arranged by size:
1799 1515 1168
MfeI C'AATT_G
Cuts at: 0 3386 4482
Size: 3386 1096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 2729 4482
Size: 1156 1573 1753
Fragments arranged by size:
1753 1573 1156
MscI TGG'CCA
Cuts at: 0 328 2678 4482
Size: 328 2350 1804
Fragments arranged by size:
2350 1804 328
MslI CAynn'nnrTG
Cuts at: 0 180 1148 2464 3869 4007 4482
Size: 180 968 1316 1405 138 475
Fragments arranged by size:
1405 1316 968 475 180 138
MspA1I CmG'CkG
Cuts at: 0 2208 2623 4482
Size: 2208 415 1859
Fragments arranged by size:
2208 1859 415
NaeI GCC'GGC
Cuts at: 0 1827 4482
Size: 1827 2655
NcoI C'CATG_G
Cuts at: 0 530 651 3026 3513 4482
Size: 530 121 2375 487 969
Fragments arranged by size:
2375 969 530 487 121
NgoMIV G'CCGG_C
Cuts at: 0 1825 4482
Size: 1825 2657
NheI G'CTAG_C
Cuts at: 0 1761 2365 4482
Size: 1761 604 2117
Fragments arranged by size:
2117 1761 604
NsiI A_TGCA'T
Cuts at: 0 301 4482
Size: 301 4181
NspI r_CATG'y
Cuts at: 0 179 1188 1890 4482
Size: 179 1009 702 2592
Fragments arranged by size:
2592 1009 702 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 643 3199 4482
Size: 643 2556 1283
Fragments arranged by size:
2556 1283 643
PfoI T'CCnGG_A
Cuts at: 0 231 381 4482
Size: 231 150 4101
Fragments arranged by size:
4101 231 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4087 4119 4482
Size: 4087 32 363
Fragments arranged by size:
4087 363 32
PpuMI rG'GwC_Cy
Cuts at: 0 1432 1493 4482
Size: 1432 61 2989
Fragments arranged by size:
2989 1432 61
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 4482
Size: 1259 32 3191
Fragments arranged by size:
3191 1259 32
PstI C_TGCA'G
Cuts at: 0 2037 2622 2639 3321 4482
Size: 2037 585 17 682 1161
Fragments arranged by size:
2037 1161 682 585 17
PvuII CAG'CTG
Cuts at: 0 2208 2623 4482
Size: 2208 415 1859
Fragments arranged by size:
2208 1859 415
SacI G_AGCT'C
Cuts at: 0 780 4482
Size: 780 3702
SanDI GG'GwC_CC
Cuts at: 0 1493 4482
Size: 1493 2989
SapI GCTCTTCn'nnn_
Cuts at: 0 1040 4482
Size: 1040 3442
SexAI A'CCwGG_T
Cuts at: 0 1239 3932 4482
Size: 1239 2693 550
Fragments arranged by size:
2693 1239 550
SfcI C'TryA_G
Cuts at: 0 1427 2033 2113 2618 2635 2896 3317 4308
Size: 1427 606 80 505 17 261 421 991
Cuts at: 4308 4482
Size: 174
Fragments arranged by size:
1427 991 606 505 421 261 174 80
17
SmaI CCC'GGG
Cuts at: 0 2162 4482
Size: 2162 2320
SmlI C'TyrA_G
Cuts at: 0 2142 4482
Size: 2142 2340
SphI G_CATG'C
Cuts at: 0 1188 4482
Size: 1188 3294
SspI AAT'ATT
Cuts at: 0 3550 4482
Size: 3550 932
StuI AGG'CCT
Cuts at: 0 659 4482
Size: 659 3823
StyI C'CwwG_G
Cuts at: 0 212 530 651 715 999 1477 1498 2985
Size: 212 318 121 64 284 478 21 1487
Cuts at: 2985 3026 3513 4138 4482
Size: 41 487 625 344
Fragments arranged by size:
1487 625 487 478 344 318 284 212
121 64 41 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1482 3220 3749 4028 4482
Size: 1482 1738 529 279 454
Fragments arranged by size:
1738 1482 529 454 279
TatI w'GTAC_w
Cuts at: 0 546 852 1266 1550 1593 3328 4413 4482
Size: 546 306 414 284 43 1735 1085 69
Fragments arranged by size:
1735 1085 546 414 306 284 69 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 3105 3129 3137 3161 4482
Size: 227 32 2846 24 8 24 1321
Fragments arranged by size:
2846 1321 227 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 0 3890 3934 4162 4482
Size: 3890 44 228 320
Fragments arranged by size:
3890 320 228 44
XbaI T'CTAG_A
Cuts at: 0 3507 3777 4482
Size: 3507 270 705
Fragments arranged by size:
3507 705 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4389 4482
Size: 4389 93
XmaI C'CCGG_G
Cuts at: 0 2160 4482
Size: 2160 2322
XmnI GAAnn'nnTTC
Cuts at: 0 499 1644 4482
Size: 499 1145 2838
Fragments arranged by size:
2838 1145 499
Enzymes that do cut:
AccI Acc65I AcuI AhdI AleI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PflMI
PfoI PpiI PpuMI PsrI PstI PvuII SacI SanDI
SapI SexAI SfcI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI ApaI
AscI AsiSI AvrII BaeI BamHI BbeI BciVI BmgBI
BplI BsaAI BsiEI BsiWI BsmBI BsrBI BssHII BstAPI
BstBI BstZ17I ClaI DrdI EagI EciI FseI FspI
FspAI HaeII HindIII HpaI KasI MluI NarI NdeI
NotI NruI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SwaI XhoI ZraI