Restriction map for
pFN21AA1417
(Linear) (Six-base) MAPSORT of: KIAA1417.seq Check: 4834 from: 1 to: 4059
KIAA1417.orf
With 182 enzymes: SgfI *
July 3, 2012 15:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 531 4059
Size: 531 3528
AccI GT'mk_AC
Cuts at: 0 3393 4059
Size: 3393 666
Acc65I G'GTAC_C
Cuts at: 0 3739 4059
Size: 3739 320
AclI AA'CG_TT
Cuts at: 0 2964 4059
Size: 2964 1095
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 755 1300 1781 2169 2290 2638 3110
Size: 64 691 545 481 388 121 348 472
Cuts at: 3110 3797 4007 4059
Size: 687 210 52
Fragments arranged by size:
691 687 545 481 472 388 348 210
121 64 52
AflIII A'CryG_T
Cuts at: 0 1877 4059
Size: 1877 2182
AhdI GACnn_n'nnGTC
Cuts at: 0 249 4059
Size: 249 3810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2183 4059
Size: 2183 1876
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2905 2937 4059
Size: 2905 32 1122
Fragments arranged by size:
2905 1122 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3088 3820 4059
Size: 3088 732 239
Fragments arranged by size:
3088 732 239
ApoI r'AATT_y
Cuts at: 0 453 556 1214 1346 1499 1610 1801 1992
Size: 453 103 658 132 153 111 191 191
Cuts at: 1992 2013 2176 2335 2641 3699 4059
Size: 21 163 159 306 1058 360
Fragments arranged by size:
1058 658 453 360 306 191 191 163
159 153 132 111 103 21
AseI AT'TA_AT
Cuts at: 0 2237 4059
Size: 2237 1822
BanI G'GyrC_C
Cuts at: 0 1841 3708 3739 4059
Size: 1841 1867 31 320
Fragments arranged by size:
1867 1841 320 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 1305 4059
Size: 1305 2754
BclI T'GATC_A
Cuts at: 0 2553 4059
Size: 2553 1506
BfrBI ATG'CAT
Cuts at: 0 3571 4059
Size: 3571 488
BmgBI CAC'GTC
Cuts at: 0 1306 2307 4059
Size: 1306 1001 1752
Fragments arranged by size:
1752 1306 1001
BmrI ACTGGGnnnn_n'
Cuts at: 0 949 3278 3835 4059
Size: 949 2329 557 224
Fragments arranged by size:
2329 949 557 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 501 635 1278 2999 4059
Size: 96 405 134 643 1721 1060
Fragments arranged by size:
1721 1060 643 405 134 96
Bpu10I CC'TnA_GC
Cuts at: 0 710 4059
Size: 710 3349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 4059
Size: 1528 2531
BsaI GGTCTCn'nnnn_
Cuts at: 0 314 999 2735 4059
Size: 314 685 1736 1324
Fragments arranged by size:
1736 1324 685 314
BsaAI yAC'GTr
Cuts at: 0 2959 4059
Size: 2959 1100
BsaBI GATnn'nnATC
Cuts at: 0 532 4059
Size: 532 3527
BsaWI w'CCGG_w
Cuts at: 0 2780 4059
Size: 2780 1279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1040 1070 4059
Size: 1040 30 2989
Fragments arranged by size:
2989 1040 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 974 3949 4059
Size: 974 2975 110
Fragments arranged by size:
2975 974 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 1712 2444 4059
Size: 6 1706 732 1615
Fragments arranged by size:
1706 1615 732 6
BsmI GAATG_Cn'
Cuts at: 0 165 1220 1685 1752 2628 3573 3588 4059
Size: 165 1055 465 67 876 945 15 471
Fragments arranged by size:
1055 945 876 471 465 165 67 15
BspHI T'CATG_A
Cuts at: 0 1689 4059
Size: 1689 2370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 579 866 4059
Size: 531 48 287 3193
Fragments arranged by size:
3193 531 287 48
BsrDI GCAATG_nn'
Cuts at: 0 265 1016 1235 2657 2804 4059
Size: 265 751 219 1422 147 1255
Fragments arranged by size:
1422 1255 751 265 219 147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 4059
Size: 546 3513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3054 4059
Size: 3054 1005
BstYI r'GATC_y
Cuts at: 0 228 369 507 954 1551 1587 2763 3360
Size: 228 141 138 447 597 36 1176 597
Cuts at: 3360 4059
Size: 699
Fragments arranged by size:
1176 699 597 597 447 228 141 138
36
BtgI C'CryG_G
Cuts at: 0 3235 3735 3830 4059
Size: 3235 500 95 229
Fragments arranged by size:
3235 500 229 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2762 4059
Size: 2762 1297
BtsI GCAGTG_nn'
Cuts at: 0 1291 1754 4059
Size: 1291 463 2305
Fragments arranged by size:
2305 1291 463
DraI TTT'AAA
Cuts at: 0 1334 3230 3687 4059
Size: 1334 1896 457 372
Fragments arranged by size:
1896 1334 457 372
DraIII CAC_nnn'GTG
Cuts at: 0 567 4059
Size: 567 3492
DrdI GACnn_nn'nnGTC
Cuts at: 0 680 4059
Size: 680 3379
EarI CTCTTCn'nnn_
Cuts at: 0 1391 1637 1726 2089 2802 3068 3983 4059
Size: 1391 246 89 363 713 266 915 76
Fragments arranged by size:
1391 915 713 363 266 246 89 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 96 501 635 755 1278 1300 1781
Size: 64 32 405 134 120 523 22 481
Cuts at: 1781 2169 2290 2638 2999 3110 3797 4007 4059
Size: 388 121 348 361 111 687 210 52
Fragments arranged by size:
687 523 481 405 388 361 348 210
134 121 120 111 64 52 32 22
EcoRI G'AATT_C
Cuts at: 0 1499 1992 4059
Size: 1499 493 2067
Fragments arranged by size:
2067 1499 493
EcoRV GAT'ATC
Cuts at: 0 2871 4059
Size: 2871 1188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3907 4059
Size: 3907 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 235 267 499 531 1040 1072
Size: 10 32 193 32 232 32 509 32
Cuts at: 1072 1417 1449 3115 3147 4059
Size: 345 32 1666 32 912
Fragments arranged by size:
1666 912 509 345 232 193 32 32
32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 1269 3077 4059
Size: 1269 1808 982
Fragments arranged by size:
1808 1269 982
KpnI G_GTAC'C
Cuts at: 0 3743 4059
Size: 3743 316
MslI CAynn'nnrTG
Cuts at: 0 30 635 2024 2298 2351 4059
Size: 30 605 1389 274 53 1708
Fragments arranged by size:
1708 1389 605 274 53 30
MspA1I CmG'CkG
Cuts at: 0 706 1618 4059
Size: 706 912 2441
Fragments arranged by size:
2441 912 706
NcoI C'CATG_G
Cuts at: 0 3235 3735 3830 4059
Size: 3235 500 95 229
Fragments arranged by size:
3235 500 229 95
NdeI CA'TA_TG
Cuts at: 0 1163 4059
Size: 1163 2896
NsiI A_TGCA'T
Cuts at: 0 3573 4059
Size: 3573 486
NspI r_CATG'y
Cuts at: 0 52 650 1881 3943 4059
Size: 52 598 1231 2062 116
Fragments arranged by size:
2062 1231 598 116 52
PasI CC'CwG_GG
Cuts at: 0 462 4059
Size: 462 3597
PciI A'CATG_T
Cuts at: 0 1877 4059
Size: 1877 2182
PflMI CCAn_nnn'nTGG
Cuts at: 0 3800 4059
Size: 3800 259
PfoI T'CCnGG_A
Cuts at: 0 517 612 3611 4059
Size: 517 95 2999 448
Fragments arranged by size:
2999 517 448 95
PsiI TTA'TAA
Cuts at: 0 2987 4059
Size: 2987 1072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1850 1882 4059
Size: 1850 32 2177
Fragments arranged by size:
2177 1850 32
PstI C_TGCA'G
Cuts at: 0 1583 1839 4059
Size: 1583 256 2220
Fragments arranged by size:
2220 1583 256
PvuII CAG'CTG
Cuts at: 0 706 1618 4059
Size: 706 912 2441
Fragments arranged by size:
2441 912 706
SapI GCTCTTCn'nnn_
Cuts at: 0 2089 4059
Size: 2089 1970
SfcI C'TryA_G
Cuts at: 0 422 1052 1579 1835 3020 4059
Size: 422 630 527 256 1185 1039
Fragments arranged by size:
1185 1039 630 527 422 256
SmlI C'TyrA_G
Cuts at: 0 1507 4059
Size: 1507 2552
SphI G_CATG'C
Cuts at: 0 52 3943 4059
Size: 52 3891 116
Fragments arranged by size:
3891 116 52
SspI AAT'ATT
Cuts at: 0 2130 2377 4059
Size: 2130 247 1682
Fragments arranged by size:
2130 1682 247
StuI AGG'CCT
Cuts at: 0 103 4059
Size: 103 3956
StyI C'CwwG_G
Cuts at: 0 3235 3494 3735 3830 4059
Size: 3235 259 241 95 229
Fragments arranged by size:
3235 259 241 229 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 4059
Size: 24 4035
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 192 4059
Size: 160 32 3867
Fragments arranged by size:
3867 160 32
XbaI T'CTAG_A
Cuts at: 0 3783 4059
Size: 3783 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2319 3569 4059
Size: 2319 1250 490
Fragments arranged by size:
2319 1250 490
XmnI GAAnn'nnTTC
Cuts at: 0 1410 1499 2378 3062 4059
Size: 1410 89 879 684 997
Fragments arranged by size:
1410 997 879 684 89
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApoI AseI BanI BbsI BclI BfrBI
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsmI BspHI BspMI BsrDI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EarI Eco57MI EcoRI EcoRV FalI Hin4I HincII
KpnI MslI MspA1I NcoI NdeI NsiI NspI PasI
PciI PflMI PfoI PsiI PsrI PstI PvuII SapI
SfcI SmlI SphI SspI StuI StyI TaqII TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmtI BplI
BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI EaeI EagI EciI EcoICRI EcoNI
EcoO109I FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI TatI Tth111I XhoI XmaI
ZraI