Restriction map for
pFN21AA1007
(Linear) (Six-base) MAPSORT of: KIAA1007.seq Check: 4517 from: 1 to: 7113
KIAA1007.orf
With 182 enzymes: SgfI *
August 19, 2010 16:05 ..
AatII G_ACGT'C
Cuts at: 0 494 2088 7113
Size: 494 1594 5025
Fragments arranged by size:
5025 1594 494
AccI GT'mk_AC
Cuts at: 0 1175 7113
Size: 1175 5938
Acc65I G'GTAC_C
Cuts at: 0 6809 7113
Size: 6809 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1401 3472 3580 3931 5671 5893 6819
Size: 1226 175 2071 108 351 1740 222 926
Cuts at: 6819 7113
Size: 294
Fragments arranged by size:
2071 1740 1226 926 351 294 222 175
108
AflII C'TTAA_G
Cuts at: 0 619 4746 7113
Size: 619 4127 2367
Fragments arranged by size:
4127 2367 619
AflIII A'CryG_T
Cuts at: 0 6443 7113
Size: 6443 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1988 3950 5762 7077 7113
Size: 1988 1962 1812 1315 36
Fragments arranged by size:
1988 1962 1812 1315 36
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4169 4201 7113
Size: 4169 32 2912
Fragments arranged by size:
4169 2912 32
AlwNI CAG_nnn'CTG
Cuts at: 0 219 3384 4194 4534 4604 5587 6186 7113
Size: 219 3165 810 340 70 983 599 927
Fragments arranged by size:
3165 983 927 810 599 340 219 70
ApoI r'AATT_y
Cuts at: 0 255 402 1056 1100 1811 2214 3358 3463
Size: 255 147 654 44 711 403 1144 105
Cuts at: 3463 3915 4561 5935 5960 6372 6741 7006 7113
Size: 452 646 1374 25 412 369 265 107
Fragments arranged by size:
1374 1144 711 654 646 452 412 403
369 265 255 147 107 105 44 25
AseI AT'TA_AT
Cuts at: 0 3194 5366 5864 6017 7113
Size: 3194 2172 498 153 1096
Fragments arranged by size:
3194 2172 1096 498 153
AvaI C'yCGr_G
Cuts at: 0 4940 7113
Size: 4940 2173
BamHI G'GATC_C
Cuts at: 0 713 2824 7113
Size: 713 2111 4289
Fragments arranged by size:
4289 2111 713
BanI G'GyrC_C
Cuts at: 0 2428 4095 5120 6809 7105 7113
Size: 2428 1667 1025 1689 296 8
Fragments arranged by size:
2428 1689 1667 1025 296 8
BanII G_rGCy'C
Cuts at: 0 760 3257 7113
Size: 760 2497 3856
Fragments arranged by size:
3856 2497 760
BbsI GAAGACnn'nnnn_
Cuts at: 0 4516 5659 6940 7113
Size: 4516 1143 1281 173
Fragments arranged by size:
4516 1281 1143 173
BbvCI CC'TCA_GC
Cuts at: 0 1372 4407 7113
Size: 1372 3035 2706
Fragments arranged by size:
3035 2706 1372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 810 844 4690 4724 5759 5793 7113
Size: 810 34 3846 34 1035 34 1320
Fragments arranged by size:
3846 1320 1035 810 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2708 6370 6600 7113
Size: 2708 3662 230 513
Fragments arranged by size:
3662 2708 513 230
BclI T'GATC_A
Cuts at: 0 1656 3587 7113
Size: 1656 1931 3526
Fragments arranged by size:
3526 1931 1656
BfrBI ATG'CAT
Cuts at: 0 1570 1617 5481 7113
Size: 1570 47 3864 1632
Fragments arranged by size:
3864 1632 1570 47
BglII A'GATC_T
Cuts at: 0 417 462 1250 5331 7113
Size: 417 45 788 4081 1782
Fragments arranged by size:
4081 1782 788 417 45
BlpI GC'TnA_GC
Cuts at: 0 3785 5729 7113
Size: 3785 1944 1384
Fragments arranged by size:
3785 1944 1384
Bme1580I G_kGCm'C
Cuts at: 0 2431 3695 6268 7023 7113
Size: 2431 1264 2573 755 90
Fragments arranged by size:
2573 2431 1264 755 90
BmgBI CAC'GTC
Cuts at: 0 1771 7113
Size: 1771 5342
BmrI ACTGGGnnnn_n'
Cuts at: 0 432 1165 2434 2688 2886 7113
Size: 432 733 1269 254 198 4227
Fragments arranged by size:
4227 1269 733 432 254 198
BmtI G_CTAG'C
Cuts at: 0 2102 7113
Size: 2102 5011
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2003 3948 5330 7113
Size: 2003 1945 1382 1783
Fragments arranged by size:
2003 1945 1783 1382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 1916 2087 3179 4861 5375 5504 6512
Size: 981 935 171 1092 1682 514 129 1008
Cuts at: 6512 6604 7113
Size: 92 509
Fragments arranged by size:
1682 1092 1008 981 935 514 509 171
129 92
Bpu10I CC'TnA_GC
Cuts at: 0 1372 2659 3038 3050 4407 4472 4892 5723
Size: 1372 1287 379 12 1357 65 420 831
Cuts at: 5723 5996 7113
Size: 273 1117
Fragments arranged by size:
1372 1357 1287 1117 831 420 379 273
65 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4871 5480 6081 7113
Size: 4871 609 601 1032
Fragments arranged by size:
4871 1032 609 601
BsaI GGTCTCn'nnnn_
Cuts at: 0 3481 5096 7113
Size: 3481 1615 2017
Fragments arranged by size:
3481 2017 1615
BsaBI GATnn'nnATC
Cuts at: 0 203 1322 3902 6530 7113
Size: 203 1119 2580 2628 583
Fragments arranged by size:
2628 2580 1119 583 203
BsaHI Gr'CG_yC
Cuts at: 0 491 2085 7113
Size: 491 1594 5028
Fragments arranged by size:
5028 1594 491
BsaWI w'CCGG_w
Cuts at: 0 727 6806 7113
Size: 727 6079 307
Fragments arranged by size:
6079 727 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 533 563 6060 6090 7113
Size: 220 30 283 30 5497 30 1023
Fragments arranged by size:
5497 1023 283 220 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 541 553 3065 7113
Size: 538 3 12 2512 4048
Fragments arranged by size:
4048 2512 538 12 3
BseYI C'CCAG_C
Cuts at: 0 338 4772 5405 6981 7082 7113
Size: 338 4434 633 1576 101 31
Fragments arranged by size:
4434 1576 633 338 101 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 853 1742 1994 2837 7113
Size: 305 548 889 252 843 4276
Fragments arranged by size:
4276 889 843 548 305 252
BsiEI CG_ry'CG
Cuts at: 0 7052 7113
Size: 7052 61
BsiHKAI G_wGCw'C
Cuts at: 0 356 668 760 993 1923 2379 3512 5457
Size: 356 312 92 233 930 456 1133 1945
Cuts at: 5457 5724 6644 6706 7113
Size: 267 920 62 407
Fragments arranged by size:
1945 1133 930 920 456 407 356 312
267 233 92 62
BsmI GAATG_Cn'
Cuts at: 0 920 1139 2227 2293 3722 4298 5047 5481
Size: 920 219 1088 66 1429 576 749 434
Cuts at: 5481 6140 6792 7113
Size: 659 652 321
Fragments arranged by size:
1429 1088 920 749 659 652 576 434
321 219 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1857 7113
Size: 1857 5256
Bsp1286I G_dGCh'C
Cuts at: 0 356 668 760 993 1923 2379 2431 3257
Size: 356 312 92 233 930 456 52 826
Cuts at: 3257 3512 3695 5457 5724 6268 6644 6706 7023
Size: 255 183 1762 267 544 376 62 317
Cuts at: 7023 7113
Size: 90
Fragments arranged by size:
1762 930 826 544 456 376 356 317
312 267 255 233 183 92 90 62
52
BspEI T'CCGG_A
Cuts at: 0 727 7113
Size: 727 6386
BspHI T'CATG_A
Cuts at: 0 4370 5357 5916 7113
Size: 4370 987 559 1197
Fragments arranged by size:
4370 1197 987 559
BsrDI GCAATG_nn'
Cuts at: 0 292 1628 3640 4722 5391 5447 5810 6461
Size: 292 1336 2012 1082 669 56 363 651
Cuts at: 6461 6662 7113
Size: 201 451
Fragments arranged by size:
2012 1336 1082 669 651 451 363 292
201 56
BsrGI T'GTAC_A
Cuts at: 0 2412 4024 7011 7113
Size: 2412 1612 2987 102
Fragments arranged by size:
2987 2412 1612 102
BssSI C'ACGA_G
Cuts at: 0 5821 7113
Size: 5821 1292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1491 4161 7113
Size: 1491 2670 2952
Fragments arranged by size:
2952 2670 1491
BstBI TT'CG_AA
Cuts at: 0 4902 7113
Size: 4902 2211
BstEII G'GTnAC_C
Cuts at: 0 3102 3144 7113
Size: 3102 42 3969
Fragments arranged by size:
3969 3102 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3755 6941 7113
Size: 3755 3186 172
Fragments arranged by size:
3755 3186 172
BstYI r'GATC_y
Cuts at: 0 417 462 713 956 1250 2824 2982 5331
Size: 417 45 251 243 294 1574 158 2349
Cuts at: 5331 5489 7113
Size: 158 1624
Fragments arranged by size:
2349 1624 1574 417 294 251 243 158
158 45
Bsu36I CC'TnA_GG
Cuts at: 0 122 5527 7113
Size: 122 5405 1586
Fragments arranged by size:
5405 1586 122
BtgI C'CryG_G
Cuts at: 0 659 2015 4031 4757 5095 7113
Size: 659 1356 2016 726 338 2018
Fragments arranged by size:
2018 2016 1356 726 659 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 191 7113
Size: 191 6922
BtsI GCAGTG_nn'
Cuts at: 0 195 957 2288 2399 4162 5815 7113
Size: 195 762 1331 111 1763 1653 1298
Fragments arranged by size:
1763 1653 1331 1298 762 195 111
ClaI AT'CG_AT
Cuts at: 0 4215 7113
Size: 4215 2898
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4853 4888 7113
Size: 4853 35 2225
Fragments arranged by size:
4853 2225 35
DraI TTT'AAA
Cuts at: 0 314 1859 2613 2868 7113
Size: 314 1545 754 255 4245
Fragments arranged by size:
4245 1545 754 314 255
DraIII CAC_nnn'GTG
Cuts at: 0 1774 5120 7113
Size: 1774 3346 1993
Fragments arranged by size:
3346 1993 1774
DrdI GACnn_nn'nnGTC
Cuts at: 0 490 2469 4072 7113
Size: 490 1979 1603 3041
Fragments arranged by size:
3041 1979 1603 490
EaeI y'GGCC_r
Cuts at: 0 2482 3224 4874 5076 5589 7113
Size: 2482 742 1650 202 513 1524
Fragments arranged by size:
2482 1650 1524 742 513 202
EarI CTCTTCn'nnn_
Cuts at: 0 301 1339 1420 3326 3388 4697 4904 7113
Size: 301 1038 81 1906 62 1309 207 2209
Fragments arranged by size:
2209 1906 1309 1038 301 207 81 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1901 7113
Size: 1901 5212
EcoICRI GAG'CTC
Cuts at: 0 758 7113
Size: 758 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 1226 1401 1916 2087 3179 3472 3580
Size: 981 245 175 515 171 1092 293 108
Cuts at: 3580 3931 4861 5375 5504 5671 5893 6512 6604
Size: 351 930 514 129 167 222 619 92
Cuts at: 6604 6819 7113
Size: 215 294
Fragments arranged by size:
1092 981 930 619 515 514 351 294
293 245 222 215 175 171 167 129
108 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3892 6259 7113
Size: 3892 2367 854
Fragments arranged by size:
3892 2367 854
EcoO109I rG'GnC_Cy
Cuts at: 0 537 4531 5880 7113
Size: 537 3994 1349 1233
Fragments arranged by size:
3994 1349 1233 537
EcoRI G'AATT_C
Cuts at: 0 402 7113
Size: 402 6711
EcoRV GAT'ATC
Cuts at: 0 6732 7113
Size: 6732 381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 600 3094 3834 7113
Size: 600 2494 740 3279
Fragments arranged by size:
3279 2494 740 600
FspI TGC'GCA
Cuts at: 0 631 6574 6668 7113
Size: 631 5943 94 445
Fragments arranged by size:
5943 631 445 94
HaeII r_GCGC'y
Cuts at: 0 6214 7113
Size: 6214 899
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1350 1382 1854 1886 2121 2153
Size: 1 32 1317 32 472 32 235 32
Cuts at: 2153 3622 3654 5355 5387 5646 5678 6099 6131
Size: 1469 32 1701 32 259 32 421 32
Cuts at: 6131 6536 6568 7113
Size: 405 32 545
Fragments arranged by size:
1701 1469 1317 545 472 421 405 259
235 32 32 32 32 32 32 32
32 32 1
HindIII A'AGCT_T
Cuts at: 0 454 1967 4787 5606 7113
Size: 454 1513 2820 819 1507
Fragments arranged by size:
2820 1513 1507 819 454
KpnI G_GTAC'C
Cuts at: 0 6813 7113
Size: 6813 300
MfeI C'AATT_G
Cuts at: 0 2025 4172 4479 6791 7113
Size: 2025 2147 307 2312 322
Fragments arranged by size:
2312 2147 2025 322 307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 2593 4141 5453 5604 5987 7113
Size: 573 2020 1548 1312 151 383 1126
Fragments arranged by size:
2020 1548 1312 1126 573 383 151
MscI TGG'CCA
Cuts at: 0 2484 3226 4876 7113
Size: 2484 742 1650 2237
Fragments arranged by size:
2484 2237 1650 742
MslI CAynn'nnrTG
Cuts at: 0 703 1002 1516 2767 3505 4853 5257 7113
Size: 703 299 514 1251 738 1348 404 1856
Fragments arranged by size:
1856 1348 1251 738 703 514 404 299
MspA1I CmG'CkG
Cuts at: 0 342 859 2176 4333 4459 5409 5587 7113
Size: 342 517 1317 2157 126 950 178 1526
Fragments arranged by size:
2157 1526 1317 950 517 342 178 126
NcoI C'CATG_G
Cuts at: 0 2015 5095 7113
Size: 2015 3080 2018
Fragments arranged by size:
3080 2018 2015
NdeI CA'TA_TG
Cuts at: 0 5085 7113
Size: 5085 2028
NheI G'CTAG_C
Cuts at: 0 2098 7113
Size: 2098 5015
NsiI A_TGCA'T
Cuts at: 0 1572 1619 5483 7113
Size: 1572 47 3864 1630
Fragments arranged by size:
3864 1630 1572 47
NspI r_CATG'y
Cuts at: 0 163 1204 1449 1621 4355 4582 4858 6004
Size: 163 1041 245 172 2734 227 276 1146
Cuts at: 6004 6447 7113
Size: 443 666
Fragments arranged by size:
2734 1146 1041 666 443 276 245 227
172 163
PciI A'CATG_T
Cuts at: 0 6443 7113
Size: 6443 670
PflMI CCAn_nnn'nTGG
Cuts at: 0 2065 2335 4194 4206 5052 5412 6744 7113
Size: 2065 270 1859 12 846 360 1332 369
Fragments arranged by size:
2065 1859 1332 846 369 360 270 12
PfoI T'CCnGG_A
Cuts at: 0 1089 3269 4187 7113
Size: 1089 2180 918 2926
Fragments arranged by size:
2926 2180 1089 918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2361 2393 4352 4384 7113
Size: 2361 32 1959 32 2729
Fragments arranged by size:
2729 2361 1959 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 537 5880 7113
Size: 537 5343 1233
Fragments arranged by size:
5343 1233 537
PshAI GACnn'nnGTC
Cuts at: 0 2973 4748 7113
Size: 2973 1775 2365
Fragments arranged by size:
2973 2365 1775
PsiI TTA'TAA
Cuts at: 0 1240 7113
Size: 1240 5873
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2154 2186 5885 5917 7113
Size: 2154 32 3699 32 1196
Fragments arranged by size:
3699 2154 1196 32 32
PstI C_TGCA'G
Cuts at: 0 2394 2606 3534 4518 5072 7113
Size: 2394 212 928 984 554 2041
Fragments arranged by size:
2394 2041 984 928 554 212
PvuII CAG'CTG
Cuts at: 0 342 859 2176 4333 4459 5409 5587 7113
Size: 342 517 1317 2157 126 950 178 1526
Fragments arranged by size:
2157 1526 1317 950 517 342 178 126
SacI G_AGCT'C
Cuts at: 0 760 7113
Size: 760 6353
SapI GCTCTTCn'nnn_
Cuts at: 0 1339 1420 3326 4904 7113
Size: 1339 81 1906 1578 2209
Fragments arranged by size:
2209 1906 1578 1339 81
SexAI A'CCwGG_T
Cuts at: 0 44 7113
Size: 44 7069
SfcI C'TryA_G
Cuts at: 0 2390 2602 3492 3530 4514 4979 5034 5068
Size: 2390 212 890 38 984 465 55 34
Cuts at: 5068 6583 7113
Size: 1515 530
Fragments arranged by size:
2390 1515 984 890 530 465 212 55
38 34
SmlI C'TyrA_G
Cuts at: 0 619 4746 4886 5495 6060 7113
Size: 619 4127 140 609 565 1053
Fragments arranged by size:
4127 1053 619 609 565 140
SphI G_CATG'C
Cuts at: 0 1621 7113
Size: 1621 5492
SspI AAT'ATT
Cuts at: 0 6205 7113
Size: 6205 908
StuI AGG'CCT
Cuts at: 0 1705 5525 6066 7113
Size: 1705 3820 541 1047
Fragments arranged by size:
3820 1705 1047 541
StyI C'CwwG_G
Cuts at: 0 1192 1530 2015 3367 3754 5095 5883 6940
Size: 1192 338 485 1352 387 1341 788 1057
Cuts at: 6940 7113
Size: 173
Fragments arranged by size:
1352 1341 1192 1057 788 485 387 338
173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2203 5550 5671 7038 7113
Size: 2203 3347 121 1367 75
Fragments arranged by size:
3347 2203 1367 121 75
TatI w'GTAC_w
Cuts at: 0 369 2412 4024 4052 5689 7011 7113
Size: 369 2043 1612 28 1637 1322 102
Fragments arranged by size:
2043 1637 1612 1322 369 102 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 948 980 7113
Size: 948 32 6133
Fragments arranged by size:
6133 948 32
Tth111I GACn'n_nGTC
Cuts at: 0 270 1868 7113
Size: 270 1598 5245
Fragments arranged by size:
5245 1598 270
XbaI T'CTAG_A
Cuts at: 0 2977 7113
Size: 2977 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 565 3752 7113
Size: 565 3187 3361
Fragments arranged by size:
3361 3187 565
XmnI GAAnn'nnTTC
Cuts at: 0 900 7113
Size: 900 6213
ZraI GAC'GTC
Cuts at: 0 492 2086 7113
Size: 492 1594 5027
Fragments arranged by size:
5027 1594 492
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AlfI AloI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HindIII KpnI MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PshAI PsiI PsrI
PstI PvuII SacI SapI SexAI SfcI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI AleI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BglI BsaAI BsiWI
BspMI BsrBI BsrFI BssHII BstZ17I EagI FseI FspAI
HincII HpaI KasI MluI NaeI NarI NgoMIV NotI
NruI PacI PasI PmeI PmlI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XhoI
XmaI