Restriction map for
pFN21AA0711
(Linear) (Six-base) MAPSORT of: KIAA0711.seq Check: 6612 from: 1 to: 1869
KIAA0711.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1369 1869
Size: 1369 500
AatII G_ACGT'C
Cuts at: 0 1799 1869
Size: 1799 70
AccI GT'mk_AC
Cuts at: 0 980 1869
Size: 980 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1869
Size: 111 1758
AfeI AGC'GCT
Cuts at: 0 1279 1471 1869
Size: 1279 192 398
Fragments arranged by size:
1279 398 192
AflIII A'CryG_T
Cuts at: 0 1379 1869
Size: 1379 490
AhdI GACnn_n'nnGTC
Cuts at: 0 351 1869
Size: 351 1518
AlwNI CAG_nnn'CTG
Cuts at: 0 40 741 1869
Size: 40 701 1128
Fragments arranged by size:
1128 701 40
ApaI G_GGCC'C
Cuts at: 0 1114 1308 1869
Size: 1114 194 561
Fragments arranged by size:
1114 561 194
AscI GG'CGCG_CC
Cuts at: 0 1037 1869
Size: 1037 832
AvaI C'yCGr_G
Cuts at: 0 46 358 367 390 901 945 1030 1325
Size: 46 312 9 23 511 44 85 295
Cuts at: 1325 1727 1847 1869
Size: 402 120 22
Fragments arranged by size:
511 402 312 295 120 85 46 44
23 22 9
BanI G'GyrC_C
Cuts at: 0 76 250 312 443 652 1101 1314 1330
Size: 76 174 62 131 209 449 213 16
Cuts at: 1330 1678 1705 1783 1861 1869
Size: 348 27 78 78 8
Fragments arranged by size:
449 348 213 209 174 131 78 78
76 62 27 16 8
BanII G_rGCy'C
Cuts at: 0 47 272 308 368 377 809 943 1114
Size: 47 225 36 60 9 432 134 171
Cuts at: 1114 1308 1393 1606 1869
Size: 194 85 213 263
Fragments arranged by size:
432 263 225 213 194 171 134 85
60 47 36 9
BbeI G_GCGC'C
Cuts at: 0 80 254 316 447 656 1105 1318 1334
Size: 80 174 62 131 209 449 213 16
Cuts at: 1334 1682 1787 1865 1869
Size: 348 105 78 4
Fragments arranged by size:
449 348 213 209 174 131 105 80
78 62 16 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 1137 1869
Size: 1137 732
BbvCI CC'TCA_GC
Cuts at: 0 165 1869
Size: 165 1704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1565 1599 1869
Size: 1565 34 270
Fragments arranged by size:
1565 270 34
BglI GCCn_nnn'nGGC
Cuts at: 0 169 830 1118 1312 1631 1643 1703 1763
Size: 169 661 288 194 319 12 60 60
Cuts at: 1763 1869
Size: 106
Fragments arranged by size:
661 319 288 194 169 106 60 60
12
BglII A'GATC_T
Cuts at: 0 1373 1869
Size: 1373 496
BlpI GC'TnA_GC
Cuts at: 0 691 1267 1869
Size: 691 576 602
Fragments arranged by size:
691 602 576
Bme1580I G_kGCm'C
Cuts at: 0 298 1114 1308 1450 1708 1869
Size: 298 816 194 142 258 161
Fragments arranged by size:
816 298 258 194 161 142
BmgBI CAC'GTC
Cuts at: 0 516 1869
Size: 516 1353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 451 811 1194 1869
Size: 183 268 360 383 675
Fragments arranged by size:
675 383 360 268 183
Bpu10I CC'TnA_GC
Cuts at: 0 165 718 1869
Size: 165 553 1151
Fragments arranged by size:
1151 553 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1396 1869
Size: 361 1035 473
Fragments arranged by size:
1035 473 361
BsaAI yAC'GTr
Cuts at: 0 1380 1869
Size: 1380 489
BsaHI Gr'CG_yC
Cuts at: 0 77 251 313 444 569 653 674 758
Size: 77 174 62 131 125 84 21 84
Cuts at: 758 1102 1315 1331 1679 1784 1796 1862 1869
Size: 344 213 16 348 105 12 66 7
Fragments arranged by size:
348 344 213 174 131 125 105 84
84 77 66 62 21 16 12 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 259 277 289 307 1435 1465
Size: 214 30 15 18 12 18 1128 30
Cuts at: 1465 1869
Size: 404
Fragments arranged by size:
1128 404 214 30 30 18 18 15
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 298 376 550 1869
Size: 280 18 78 174 1319
Fragments arranged by size:
1319 280 174 78 18
BseYI C'CCAG_C
Cuts at: 0 1301 1779 1869
Size: 1301 478 90
Fragments arranged by size:
1301 478 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1473 1869
Size: 547 926 396
Fragments arranged by size:
926 547 396
BsiEI CG_ry'CG
Cuts at: 0 822 876 977 1001 1122 1299 1478 1869
Size: 822 54 101 24 121 177 179 391
Fragments arranged by size:
822 391 179 177 121 101 54 24
BsiHKAI G_wGCw'C
Cuts at: 0 8 272 1811 1869
Size: 8 264 1539 58
Fragments arranged by size:
1539 264 58 8
Bsp1286I G_dGCh'C
Cuts at: 0 8 47 272 298 308 368 377 809
Size: 8 39 225 26 10 60 9 432
Cuts at: 809 943 1114 1308 1393 1450 1606 1708 1811
Size: 134 171 194 85 57 156 102 103
Cuts at: 1811 1869
Size: 58
Fragments arranged by size:
432 225 194 171 156 134 103 102
85 60 58 57 39 26 10 9
8
BspHI T'CATG_A
Cuts at: 0 773 1869
Size: 773 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 854 1369 1869
Size: 854 515 500
Fragments arranged by size:
854 515 500
BsrBI CCG'CTC
Cuts at: 0 225 840 1523 1559 1869
Size: 225 615 683 36 310
Fragments arranged by size:
683 615 310 225 36
BsrFI r'CCGG_y
Cuts at: 0 238 446 625 871 906 929 1041 1741
Size: 238 208 179 246 35 23 112 700
Cuts at: 1741 1869
Size: 128
Fragments arranged by size:
700 246 238 208 179 128 112 35
23
BssHII G'CGCG_C
Cuts at: 0 288 453 455 478 497 499 501 629
Size: 288 165 2 23 19 2 2 128
Cuts at: 629 631 663 867 918 961 1037 1124 1126
Size: 2 32 204 51 43 76 87 2
Cuts at: 1126 1201 1290 1869
Size: 75 89 579
Fragments arranged by size:
579 288 204 165 128 89 87 76
75 51 43 32 23 19 2 2
2 2 2
BstEII G'GTnAC_C
Cuts at: 0 1234 1869
Size: 1234 635
BstYI r'GATC_y
Cuts at: 0 1373 1869
Size: 1373 496
Bsu36I CC'TnA_GG
Cuts at: 0 281 1869
Size: 281 1588
BtgI C'CryG_G
Cuts at: 0 211 308 677 893 1106 1163 1247 1310
Size: 211 97 369 216 213 57 84 63
Cuts at: 1310 1340 1365 1423 1530 1640 1869
Size: 30 25 58 107 110 229
Fragments arranged by size:
369 229 216 213 211 110 107 97
84 63 58 57 30 25
BtsI GCAGTG_nn'
Cuts at: 0 235 1581 1603 1869
Size: 235 1346 22 266
Fragments arranged by size:
1346 266 235 22
DraIII CAC_nnn'GTG
Cuts at: 0 12 1869
Size: 12 1857
DrdI GACnn_nn'nnGTC
Cuts at: 0 976 1869
Size: 976 893
EaeI y'GGCC_r
Cuts at: 0 182 611 623 698 725 819 873 890
Size: 182 429 12 75 27 94 54 17
Cuts at: 890 974 998 1119 1160 1475 1589 1869
Size: 84 24 121 41 315 114 280
Fragments arranged by size:
429 315 280 182 121 114 94 84
75 54 41 27 24 17 12
EagI C'GGCC_G
Cuts at: 0 819 873 974 998 1119 1475 1869
Size: 819 54 101 24 121 356 394
Fragments arranged by size:
819 394 356 121 101 54 24
EarI CTCTTCn'nnn_
Cuts at: 0 134 296 1087 1402 1869
Size: 134 162 791 315 467
Fragments arranged by size:
791 467 315 162 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 1869
Size: 187 1682
EcoICRI GAG'CTC
Cuts at: 0 270 1869
Size: 270 1599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 183 451 811 1194 1869
Size: 111 72 268 360 383 675
Fragments arranged by size:
675 383 360 268 111 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 1869
Size: 1195 674
EcoO109I rG'GnC_Cy
Cuts at: 0 345 714 843 1111 1187 1305 1869
Size: 345 369 129 268 76 118 564
Fragments arranged by size:
564 369 345 268 129 118 76
HaeII r_GCGC'y
Cuts at: 0 80 254 316 447 550 656 901 1105
Size: 80 174 62 131 103 106 245 204
Cuts at: 1105 1181 1281 1318 1334 1473 1682 1716 1780
Size: 76 100 37 16 139 209 34 64
Cuts at: 1780 1787 1865 1869
Size: 7 78 4
Fragments arranged by size:
245 209 204 174 139 131 106 103
100 80 78 76 64 62 37 34
16 7 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 337 369 1869
Size: 259 32 46 32 1500
Fragments arranged by size:
1500 259 46 32 32
KasI G'GCGC_C
Cuts at: 0 76 250 312 443 652 1101 1314 1330
Size: 76 174 62 131 209 449 213 16
Cuts at: 1330 1678 1783 1861 1869
Size: 348 105 78 8
Fragments arranged by size:
449 348 213 209 174 131 105 78
76 62 16 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1869
Size: 1160 709
MscI TGG'CCA
Cuts at: 0 700 1591 1869
Size: 700 891 278
Fragments arranged by size:
891 700 278
MspA1I CmG'CkG
Cuts at: 0 169 203 213 310 578 645 690 738
Size: 169 34 10 97 268 67 45 48
Cuts at: 738 895 1108 1172 1212 1301 1320 1425 1532
Size: 157 213 64 40 89 19 105 107
Cuts at: 1532 1661 1869
Size: 129 208
Fragments arranged by size:
268 213 208 169 157 129 107 105
97 89 67 64 48 45 40 34
19 10
NaeI GCC'GGC
Cuts at: 0 240 448 627 873 908 931 1043 1743
Size: 240 208 179 246 35 23 112 700
Cuts at: 1743 1869
Size: 126
Fragments arranged by size:
700 246 240 208 179 126 112 35
23
NarI GG'CG_CC
Cuts at: 0 77 251 313 444 653 1102 1315 1331
Size: 77 174 62 131 209 449 213 16
Cuts at: 1331 1679 1784 1862 1869
Size: 348 105 78 7
Fragments arranged by size:
449 348 213 209 174 131 105 78
77 62 16 7
NgoMIV G'CCGG_C
Cuts at: 0 238 446 625 871 906 929 1041 1741
Size: 238 208 179 246 35 23 112 700
Cuts at: 1741 1869
Size: 128
Fragments arranged by size:
700 246 238 208 179 128 112 35
23
PasI CC'CwG_GG
Cuts at: 0 34 1869
Size: 34 1835
PflMI CCAn_nnn'nTGG
Cuts at: 0 789 888 1587 1598 1869
Size: 789 99 699 11 271
Fragments arranged by size:
789 699 271 99 11
PpuMI rG'GwC_Cy
Cuts at: 0 345 714 843 1869
Size: 345 369 129 1026
Fragments arranged by size:
1026 369 345 129
PspOMI G'GGCC_C
Cuts at: 0 1110 1304 1869
Size: 1110 194 565
Fragments arranged by size:
1110 565 194
PstI C_TGCA'G
Cuts at: 0 106 644 1652 1869
Size: 106 538 1008 217
Fragments arranged by size:
1008 538 217 106
PvuII CAG'CTG
Cuts at: 0 645 690 1172 1212 1869
Size: 645 45 482 40 657
Fragments arranged by size:
657 645 482 45 40
SacI G_AGCT'C
Cuts at: 0 272 1869
Size: 272 1597
SacII CC_GC'GG
Cuts at: 0 214 311 896 1109 1426 1533 1869
Size: 214 97 585 213 317 107 336
Fragments arranged by size:
585 336 317 214 213 107 97
SapI GCTCTTCn'nnn_
Cuts at: 0 296 1869
Size: 296 1573
SexAI A'CCwGG_T
Cuts at: 0 422 1139 1869
Size: 422 717 730
Fragments arranged by size:
730 717 422
SfcI C'TryA_G
Cuts at: 0 102 573 640 1648 1869
Size: 102 471 67 1008 221
Fragments arranged by size:
1008 471 221 102 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1118 1643 1869
Size: 1118 525 226
Fragments arranged by size:
1118 525 226
SfoI GGC'GCC
Cuts at: 0 78 252 314 445 654 1103 1316 1332
Size: 78 174 62 131 209 449 213 16
Cuts at: 1332 1680 1785 1863 1869
Size: 348 105 78 6
Fragments arranged by size:
449 348 213 209 174 131 105 78
78 62 16 6
SgrAI Cr'CCGG_yG
Cuts at: 0 1041 1869
Size: 1041 828
SmaI CCC'GGG
Cuts at: 0 48 369 392 1327 1729 1869
Size: 48 321 23 935 402 140
Fragments arranged by size:
935 402 321 140 48 23
SmlI C'TyrA_G
Cuts at: 0 340 1411 1869
Size: 340 1071 458
Fragments arranged by size:
1071 458 340
StyI C'CwwG_G
Cuts at: 0 1790 1869
Size: 1790 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 277 309 1435 1467 1869
Size: 214 32 31 32 1126 32 402
Fragments arranged by size:
1126 402 214 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 1141 1869
Size: 1141 728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 1869
Size: 675 1194
XmaI C'CCGG_G
Cuts at: 0 46 367 390 1325 1727 1869
Size: 46 321 23 935 402 142
Fragments arranged by size:
935 402 321 142 46 23
ZraI GAC'GTC
Cuts at: 0 1797 1869
Size: 1797 72
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlwNI
ApaI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstEII
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MspA1I NaeI NarI NgoMIV PasI
PflMI PpuMI PspOMI PstI PvuII SacI SacII SapI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StyI
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AlfI AloI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BmrI BmtI BplI BsaI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI XbaI XhoI XmnI