Restriction map for
pFN21AA0395
(Linear) (Six-base) MAPSORT of: KIAA0395.seq Check: 7718 from: 1 to: 2868
KIAA0395.orf
With 182 enzymes: SgfI *
March 22, 2012 15:29 ..
AatII G_ACGT'C
Cuts at: 0 2853 2868
Size: 2853 15
AccI GT'mk_AC
Cuts at: 0 655 2868
Size: 655 2213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 530 602 976 1090 2206 2225 2476
Size: 122 408 72 374 114 1116 19 251
Cuts at: 2476 2868
Size: 392
Fragments arranged by size:
1116 408 392 374 251 122 114 72
19
AflIII A'CryG_T
Cuts at: 0 388 2868
Size: 388 2480
AhdI GACnn_n'nnGTC
Cuts at: 0 1276 2868
Size: 1276 1592
AleI CACnn'nnGTG
Cuts at: 0 1585 2868
Size: 1585 1283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2663 2868
Size: 2663 205
AlwNI CAG_nnn'CTG
Cuts at: 0 120 867 2868
Size: 120 747 2001
Fragments arranged by size:
2001 747 120
ApaI G_GGCC'C
Cuts at: 0 767 2710 2868
Size: 767 1943 158
Fragments arranged by size:
1943 767 158
ApoI r'AATT_y
Cuts at: 0 593 2868
Size: 593 2275
BanI G'GyrC_C
Cuts at: 0 1368 1814 2239 2868
Size: 1368 446 425 629
Fragments arranged by size:
1368 629 446 425
BanII G_rGCy'C
Cuts at: 0 503 767 1001 1663 1860 1878 2546 2626
Size: 503 264 234 662 197 18 668 80
Cuts at: 2626 2710 2726 2795 2822 2868
Size: 84 16 69 27 46
Fragments arranged by size:
668 662 503 264 234 197 84 80
69 46 27 18 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 2489 2868
Size: 52 2437 379
Fragments arranged by size:
2437 379 52
BbvCI CC'TCA_GC
Cuts at: 0 1151 2006 2024 2060 2868
Size: 1151 855 18 36 808
Fragments arranged by size:
1151 855 808 36 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 1037 2868
Size: 1037 1831
BfrBI ATG'CAT
Cuts at: 0 32 2868
Size: 32 2836
BglII A'GATC_T
Cuts at: 0 1046 2868
Size: 1046 1822
Bme1580I G_kGCm'C
Cuts at: 0 767 2710 2868
Size: 767 1943 158
Fragments arranged by size:
1943 767 158
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 497 829 1422 2868
Size: 34 463 332 593 1446
Fragments arranged by size:
1446 593 463 332 34
BmtI G_CTAG'C
Cuts at: 0 1484 2868
Size: 1484 1384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2052 2829 2868
Size: 2052 777 39
Fragments arranged by size:
2052 777 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 805 1647 1700 1900 2868
Size: 680 125 842 53 200 968
Fragments arranged by size:
968 842 680 200 125 53
Bpu10I CC'TnA_GC
Cuts at: 0 907 1151 1862 2006 2024 2060 2098 2868
Size: 907 244 711 144 18 36 38 770
Fragments arranged by size:
907 770 711 244 144 38 36 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1027 2868
Size: 589 438 1841
Fragments arranged by size:
1841 589 438
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 2005 2109 2662 2868
Size: 85 1920 104 553 206
Fragments arranged by size:
1920 553 206 104 85
BsaHI Gr'CG_yC
Cuts at: 0 2850 2868
Size: 2850 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 465 495 534 564 1524 1554 2368 2398
Size: 465 30 39 30 960 30 814 30
Cuts at: 2398 2868
Size: 470
Fragments arranged by size:
960 814 470 465 39 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1111 1426 2023 2041 2065 2680 2868
Size: 1111 315 597 18 24 615 188
Fragments arranged by size:
1111 615 597 315 188 24 18
BseYI C'CCAG_C
Cuts at: 0 80 110 166 1450 2134 2235 2318 2627
Size: 80 30 56 1284 684 101 83 309
Cuts at: 2627 2868
Size: 241
Fragments arranged by size:
1284 684 309 241 101 83 80 56
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 2868
Size: 360 2508
BsiHKAI G_wGCw'C
Cuts at: 0 211 299 475 1001 2546 2626 2726 2868
Size: 211 88 176 526 1545 80 100 142
Fragments arranged by size:
1545 526 211 176 142 100 88 80
BsmI GAATG_Cn'
Cuts at: 0 914 1760 2007 2037 2868
Size: 914 846 247 30 831
Fragments arranged by size:
914 846 831 247 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1980 2868
Size: 1980 888
Bsp1286I G_dGCh'C
Cuts at: 0 211 299 475 503 767 1001 1663 1860
Size: 211 88 176 28 264 234 662 197
Cuts at: 1860 1878 2546 2626 2710 2726 2795 2822 2868
Size: 18 668 80 84 16 69 27 46
Fragments arranged by size:
668 662 264 234 211 197 176 88
84 80 69 46 28 27 18 16
BspHI T'CATG_A
Cuts at: 0 557 1506 2868
Size: 557 949 1362
Fragments arranged by size:
1362 949 557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1765 2435 2600 2622 2868
Size: 1765 670 165 22 246
Fragments arranged by size:
1765 670 246 165 22
BsrBI CCG'CTC
Cuts at: 0 1225 2868
Size: 1225 1643
BsrDI GCAATG_nn'
Cuts at: 0 1206 2522 2868
Size: 1206 1316 346
Fragments arranged by size:
1316 1206 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 120 139 338 2334 2868
Size: 120 19 199 1996 534
Fragments arranged by size:
1996 534 199 120 19
BstEII G'GTnAC_C
Cuts at: 0 1017 1752 2868
Size: 1017 735 1116
Fragments arranged by size:
1116 1017 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2679 2710 2868
Size: 2679 31 158
Fragments arranged by size:
2679 158 31
BstYI r'GATC_y
Cuts at: 0 117 253 1046 2868
Size: 117 136 793 1822
Fragments arranged by size:
1822 793 136 117
Bsu36I CC'TnA_GG
Cuts at: 0 362 1094 1748 2194 2252 2804 2868
Size: 362 732 654 446 58 552 64
Fragments arranged by size:
732 654 552 446 362 64 58
BtgI C'CryG_G
Cuts at: 0 872 935 2410 2678 2868
Size: 872 63 1475 268 190
Fragments arranged by size:
1475 872 268 190 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1684 2868
Size: 1684 1184
BtsI GCAGTG_nn'
Cuts at: 0 153 339 865 1641 2647 2700 2868
Size: 153 186 526 776 1006 53 168
Fragments arranged by size:
1006 776 526 186 168 153 53
DraIII CAC_nnn'GTG
Cuts at: 0 1242 1385 2868
Size: 1242 143 1483
Fragments arranged by size:
1483 1242 143
EaeI y'GGCC_r
Cuts at: 0 2 191 425 875 1295 2087 2362 2413
Size: 2 189 234 450 420 792 275 51
Cuts at: 2413 2463 2868
Size: 50 405
Fragments arranged by size:
792 450 420 405 275 234 189 51
50 2
EarI CTCTTCn'nnn_
Cuts at: 0 1232 1910 2042 2072 2579 2868
Size: 1232 678 132 30 507 289
Fragments arranged by size:
1232 678 507 289 132 30
EcoICRI GAG'CTC
Cuts at: 0 999 2544 2624 2724 2868
Size: 999 1545 80 100 144
Fragments arranged by size:
1545 999 144 100 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 530 602 680 805 976 1090 1647
Size: 122 408 72 78 125 171 114 557
Cuts at: 1647 1700 1900 2206 2225 2476 2868
Size: 53 200 306 19 251 392
Fragments arranged by size:
557 408 392 306 251 200 171 125
122 114 78 72 53 19
EcoO109I rG'GnC_Cy
Cuts at: 0 106 764 882 1743 2589 2706 2707 2868
Size: 106 658 118 861 846 117 1 161
Fragments arranged by size:
861 846 658 161 118 117 106 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 981 1489 2868
Size: 981 508 1379
Fragments arranged by size:
1379 981 508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 1011 1043 1262 1294 1524 1556
Size: 238 32 741 32 219 32 230 32
Cuts at: 1556 2248 2280 2368 2400 2840 2868
Size: 692 32 88 32 440 28
Fragments arranged by size:
741 692 440 238 230 219 88 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 656 2868
Size: 656 2212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1522 1789 2868
Size: 943 579 267 1079
Fragments arranged by size:
1079 943 579 267
MscI TGG'CCA
Cuts at: 0 4 193 427 1297 2089 2364 2868
Size: 4 189 234 870 792 275 504
Fragments arranged by size:
870 792 504 275 234 189 4
MslI CAynn'nnrTG
Cuts at: 0 1585 2868
Size: 1585 1283
MspA1I CmG'CkG
Cuts at: 0 88 796 1085 1405 1515 2325 2868
Size: 88 708 289 320 110 810 543
Fragments arranged by size:
810 708 543 320 289 110 88
NcoI C'CATG_G
Cuts at: 0 935 2868
Size: 935 1933
NdeI CA'TA_TG
Cuts at: 0 284 2868
Size: 284 2584
NheI G'CTAG_C
Cuts at: 0 1480 2868
Size: 1480 1388
NsiI A_TGCA'T
Cuts at: 0 34 2868
Size: 34 2834
NspI r_CATG'y
Cuts at: 0 392 844 2868
Size: 392 452 2024
Fragments arranged by size:
2024 452 392
PasI CC'CwG_GG
Cuts at: 0 1555 2868
Size: 1555 1313
PciI A'CATG_T
Cuts at: 0 388 2868
Size: 388 2480
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 630 2148 2868
Size: 276 354 1518 720
Fragments arranged by size:
1518 720 354 276
PpuMI rG'GwC_Cy
Cuts at: 0 106 1743 2589 2868
Size: 106 1637 846 279
Fragments arranged by size:
1637 846 279 106
PshAI GACnn'nnGTC
Cuts at: 0 1345 2868
Size: 1345 1523
PspOMI G'GGCC_C
Cuts at: 0 763 2706 2868
Size: 763 1943 162
Fragments arranged by size:
1943 763 162
PstI C_TGCA'G
Cuts at: 0 2552 2597 2868
Size: 2552 45 271
Fragments arranged by size:
2552 271 45
PvuII CAG'CTG
Cuts at: 0 796 1085 1405 1515 2868
Size: 796 289 320 110 1353
Fragments arranged by size:
1353 796 320 289 110
SacI G_AGCT'C
Cuts at: 0 1001 2546 2626 2726 2868
Size: 1001 1545 80 100 142
Fragments arranged by size:
1545 1001 142 100 80
SalI G'TCGA_C
Cuts at: 0 654 2868
Size: 654 2214
SapI GCTCTTCn'nnn_
Cuts at: 0 1232 2868
Size: 1232 1636
SbfI CC_TGCA'GG
Cuts at: 0 2552 2868
Size: 2552 316
ScaI AGT'ACT
Cuts at: 0 174 2868
Size: 174 2694
SfcI C'TryA_G
Cuts at: 0 2548 2593 2868
Size: 2548 45 275
Fragments arranged by size:
2548 275 45
SmlI C'TyrA_G
Cuts at: 0 604 1042 2868
Size: 604 438 1826
Fragments arranged by size:
1826 604 438
SphI G_CATG'C
Cuts at: 0 844 2868
Size: 844 2024
StuI AGG'CCT
Cuts at: 0 640 2868
Size: 640 2228
StyI C'CwwG_G
Cuts at: 0 878 935 1739 2868
Size: 878 57 804 1129
Fragments arranged by size:
1129 878 804 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 326 622 1386 2227 2737 2868
Size: 326 296 764 841 510 131
Fragments arranged by size:
841 764 510 326 296 131
TatI w'GTAC_w
Cuts at: 0 172 2868
Size: 172 2696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 2820 2852 2868
Size: 465 32 2323 32 16
Fragments arranged by size:
2323 465 32 32 16
XmnI GAAnn'nnTTC
Cuts at: 0 964 1549 1621 2868
Size: 964 585 72 1247
Fragments arranged by size:
1247 964 585 72
ZraI GAC'GTC
Cuts at: 0 2851 2868
Size: 2851 17
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI BanI BanII BbsI BbvCI BciVI BfrBI
BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PasI PciI PflMI PpuMI PshAI PspOMI PstI PvuII
SacI SalI SapI SbfI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BclI BglI BlpI BmgBI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NotI
NruI PacI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SanDI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmaI