Restriction map for
pFN21ASDA0378
(Linear) (Six-base) MAPSORT of: KIAA0378.seq Check: 9659 from: 1 to: 2871
KIAA0378.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1081 1336 1447 1615 2871
Size: 433 648 255 111 168 1256
Fragments arranged by size:
1256 648 433 255 168 111
AfeI AGC'GCT
Cuts at: 0 1795 2871
Size: 1795 1076
AflII C'TTAA_G
Cuts at: 0 568 2871
Size: 568 2303
AgeI A'CCGG_T
Cuts at: 0 2180 2871
Size: 2180 691
AlwNI CAG_nnn'CTG
Cuts at: 0 1003 1741 2445 2487 2871
Size: 1003 738 704 42 384
Fragments arranged by size:
1003 738 704 384 42
ApoI r'AATT_y
Cuts at: 0 1389 1577 2871
Size: 1389 188 1294
Fragments arranged by size:
1389 1294 188
AvaI C'yCGr_G
Cuts at: 0 899 2871
Size: 899 1972
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 169 202 2871
Size: 169 33 2669
Fragments arranged by size:
2669 169 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 850 1303 2375 2437 2871
Size: 850 453 1072 62 434
Fragments arranged by size:
1072 850 453 434 62
BbvCI CC'TCA_GC
Cuts at: 0 1378 2871
Size: 1378 1493
BclI T'GATC_A
Cuts at: 0 195 1308 2871
Size: 195 1113 1563
Fragments arranged by size:
1563 1113 195
BfrBI ATG'CAT
Cuts at: 0 1948 2871
Size: 1948 923
BglII A'GATC_T
Cuts at: 0 444 2871
Size: 444 2427
Bme1580I G_kGCm'C
Cuts at: 0 235 2871
Size: 235 2636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2276 2871
Size: 2276 595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 709 985 1111 2067 2209 2269 2446
Size: 576 133 276 126 956 142 60 177
Cuts at: 2446 2563 2575 2871
Size: 117 12 296
Fragments arranged by size:
956 576 296 276 177 142 133 126
117 60 12
Bpu10I CC'TnA_GC
Cuts at: 0 563 821 1378 2871
Size: 563 258 557 1493
Fragments arranged by size:
1493 563 557 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 606 946 1210 2182 2287 2653 2871
Size: 604 2 340 264 972 105 366 218
Fragments arranged by size:
972 604 366 340 264 218 105 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 701 2871
Size: 74 627 2170
Fragments arranged by size:
2170 627 74
BsaBI GATnn'nnATC
Cuts at: 0 2756 2871
Size: 2756 115
BsaHI Gr'CG_yC
Cuts at: 0 2134 2871
Size: 2134 737
BsaWI w'CCGG_w
Cuts at: 0 491 2180 2519 2838 2871
Size: 491 1689 339 319 33
Fragments arranged by size:
1689 491 339 319 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 2871
Size: 266 30 2575
Fragments arranged by size:
2575 266 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 62 478 712 761 793 2544 2560 2869
Size: 62 416 234 49 32 1751 16 309
Cuts at: 2869 2871
Size: 2
Fragments arranged by size:
1751 416 309 234 62 49 32 16
2
BseYI C'CCAG_C
Cuts at: 0 455 2871
Size: 455 2416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1727 2871
Size: 1727 1144
BsiHKAI G_wGCw'C
Cuts at: 0 758 1421 2871
Size: 758 663 1450
Fragments arranged by size:
1450 758 663
BsmI GAATG_Cn'
Cuts at: 0 2424 2871
Size: 2424 447
Bsp1286I G_dGCh'C
Cuts at: 0 235 758 1421 2871
Size: 235 523 663 1450
Fragments arranged by size:
1450 663 523 235
BspEI T'CCGG_A
Cuts at: 0 491 2519 2838 2871
Size: 491 2028 319 33
Fragments arranged by size:
2028 491 319 33
BspHI T'CATG_A
Cuts at: 0 198 2871
Size: 198 2673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 2871
Size: 411 2460
BsrBI CCG'CTC
Cuts at: 0 751 2871
Size: 751 2120
BsrDI GCAATG_nn'
Cuts at: 0 2606 2871
Size: 2606 265
BsrFI r'CCGG_y
Cuts at: 0 1570 2180 2871
Size: 1570 610 691
Fragments arranged by size:
1570 691 610
BstAPI GCAn_nnn'nTGC
Cuts at: 0 166 2578 2608 2871
Size: 166 2412 30 263
Fragments arranged by size:
2412 263 166 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 2871
Size: 963 1908
BstYI r'GATC_y
Cuts at: 0 49 444 526 1185 1199 2192 2549 2871
Size: 49 395 82 659 14 993 357 322
Fragments arranged by size:
993 659 395 357 322 82 49 14
BtgI C'CryG_G
Cuts at: 0 290 2871
Size: 290 2581
ClaI AT'CG_AT
Cuts at: 0 2796 2871
Size: 2796 75
EaeI y'GGCC_r
Cuts at: 0 1568 2294 2489 2510 2871
Size: 1568 726 195 21 361
Fragments arranged by size:
1568 726 361 195 21
EarI CTCTTCn'nnn_
Cuts at: 0 590 1244 1547 1832 2871
Size: 590 654 303 285 1039
Fragments arranged by size:
1039 654 590 303 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1005 2871
Size: 1005 1866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 576 709 985 1081 1111 1336 1447
Size: 433 143 133 276 96 30 225 111
Cuts at: 1447 1615 2067 2209 2269 2446 2563 2575 2871
Size: 168 452 142 60 177 117 12 296
Fragments arranged by size:
452 433 296 276 225 177 168 143
142 133 117 111 96 60 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2200 2871
Size: 2200 671
EcoO109I rG'GnC_Cy
Cuts at: 0 402 678 1542 2831 2871
Size: 402 276 864 1289 40
Fragments arranged by size:
1289 864 402 276 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1447 1678 2593 2871
Size: 1447 231 915 278
Fragments arranged by size:
1447 915 278 231
FspI TGC'GCA
Cuts at: 0 2359 2871
Size: 2359 512
HaeII r_GCGC'y
Cuts at: 0 1797 2871
Size: 1797 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 991 1023 1055 1107 1139 1831
Size: 266 32 693 32 32 52 32 692
Cuts at: 1831 1863 1903 1935 2871
Size: 32 40 32 936
Fragments arranged by size:
936 693 692 266 52 40 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 0 668 2871
Size: 668 2203
HindIII A'AGCT_T
Cuts at: 0 1366 2871
Size: 1366 1505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 2300 2871
Size: 1322 978 571
Fragments arranged by size:
1322 978 571
MscI TGG'CCA
Cuts at: 0 2296 2512 2871
Size: 2296 216 359
Fragments arranged by size:
2296 359 216
MslI CAynn'nnrTG
Cuts at: 0 289 335 1141 1414 2871
Size: 289 46 806 273 1457
Fragments arranged by size:
1457 806 289 273 46
MspA1I CmG'CkG
Cuts at: 0 459 1314 2541 2871
Size: 459 855 1227 330
Fragments arranged by size:
1227 855 459 330
NcoI C'CATG_G
Cuts at: 0 290 2871
Size: 290 2581
NdeI CA'TA_TG
Cuts at: 0 2270 2863 2871
Size: 2270 593 8
Fragments arranged by size:
2270 593 8
NsiI A_TGCA'T
Cuts at: 0 1950 2871
Size: 1950 921
NspI r_CATG'y
Cuts at: 0 1948 2871
Size: 1948 923
PfoI T'CCnGG_A
Cuts at: 0 2083 2552 2871
Size: 2083 469 319
Fragments arranged by size:
2083 469 319
PpuMI rG'GwC_Cy
Cuts at: 0 402 1542 2871
Size: 402 1140 1329
Fragments arranged by size:
1329 1140 402
PstI C_TGCA'G
Cuts at: 0 473 2871
Size: 473 2398
PvuII CAG'CTG
Cuts at: 0 459 1314 2541 2871
Size: 459 855 1227 330
Fragments arranged by size:
1227 855 459 330
SexAI A'CCwGG_T
Cuts at: 0 398 2871
Size: 398 2473
SfcI C'TryA_G
Cuts at: 0 469 661 1537 2871
Size: 469 192 876 1334
Fragments arranged by size:
1334 876 469 192
SmlI C'TyrA_G
Cuts at: 0 568 585 619 925 1189 2197 2302 2668
Size: 568 17 34 306 264 1008 105 366
Cuts at: 2668 2871
Size: 203
Fragments arranged by size:
1008 568 366 306 264 203 105 34
17
SpeI A'CTAG_T
Cuts at: 0 2688 2871
Size: 2688 183
SspI AAT'ATT
Cuts at: 0 309 2070 2871
Size: 309 1761 801
Fragments arranged by size:
1761 801 309
StyI C'CwwG_G
Cuts at: 0 64 290 2871
Size: 64 226 2581
Fragments arranged by size:
2581 226 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 2699 2871
Size: 790 1909 172
Fragments arranged by size:
1909 790 172
TatI w'GTAC_w
Cuts at: 0 2691 2871
Size: 2691 180
Tth111I GACn'n_nGTC
Cuts at: 0 129 2871
Size: 129 2742
XbaI T'CTAG_A
Cuts at: 0 1998 2871
Size: 1998 873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 733 2442 2800 2871
Size: 733 1709 358 71
Fragments arranged by size:
1709 733 358 71
Enzymes that do cut:
AcuI AfeI AflII AgeI AlwNI ApoI AvaI BaeI
BbsI BbvCI BclI BfrBI BglII Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI ClaI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PfoI PpuMI PstI PvuII
SexAI SfcI SmlI SpeI SspI StyI TaqII TatI
Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BanII BbeI BcgI BciVI BglI BlpI
BmgBI BmrI BmtI BsaAI BsiEI BsiWI BsmBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FseI FspAI HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TstI XhoI XmaI XmnI
ZraI