Restriction map for
pFN21AA0213
(Linear) (Six-base) MAPSORT of: KIAA0213.seq Check: 5992 from: 1 to: 4674
KIAA0213.orf
With 182 enzymes: SgfI *
March 22, 2012 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2811 3934 4674
Size: 2811 1123 740
Fragments arranged by size:
2811 1123 740
AccI GT'mk_AC
Cuts at: 0 908 1554 1589 3938 4674
Size: 908 646 35 2349 736
Fragments arranged by size:
2349 908 736 646 35
Acc65I G'GTAC_C
Cuts at: 0 244 1410 3459 3773 4674
Size: 244 1166 2049 314 901
Fragments arranged by size:
2049 1166 901 314 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1105 1621 1831 1912 1996 2422 2989
Size: 944 161 516 210 81 84 426 567
Cuts at: 2989 4138 4403 4586 4674
Size: 1149 265 183 88
Fragments arranged by size:
1149 944 567 516 426 265 210 183
161 88 84 81
AflIII A'CryG_T
Cuts at: 0 3029 4161 4674
Size: 3029 1132 513
Fragments arranged by size:
3029 1132 513
AhdI GACnn_n'nnGTC
Cuts at: 0 650 1452 2933 4674
Size: 650 802 1481 1741
Fragments arranged by size:
1741 1481 802 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1931 4674
Size: 1931 2743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1829 1861 3016 3048 4674
Size: 1829 32 1155 32 1626
Fragments arranged by size:
1829 1626 1155 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 601 2967 4674
Size: 601 2366 1707
Fragments arranged by size:
2366 1707 601
ApoI r'AATT_y
Cuts at: 0 1251 1714 2190 2299 2326 2491 2561 3043
Size: 1251 463 476 109 27 165 70 482
Cuts at: 3043 4674
Size: 1631
Fragments arranged by size:
1631 1251 482 476 463 165 109 70
27
AseI AT'TA_AT
Cuts at: 0 884 4276 4674
Size: 884 3392 398
Fragments arranged by size:
3392 884 398
AvaI C'yCGr_G
Cuts at: 0 112 130 4674
Size: 112 18 4544
Fragments arranged by size:
4544 112 18
AvrII C'CTAG_G
Cuts at: 0 1405 4674
Size: 1405 3269
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 947 980 4674
Size: 947 33 3694
Fragments arranged by size:
3694 947 33
BamHI G'GATC_C
Cuts at: 0 2123 4674
Size: 2123 2551
BanI G'GyrC_C
Cuts at: 0 244 1410 2752 3459 3773 4246 4377 4674
Size: 244 1166 1342 707 314 473 131 297
Fragments arranged by size:
1342 1166 707 473 314 297 244 131
BanII G_rGCy'C
Cuts at: 0 2400 2411 2730 4079 4674
Size: 2400 11 319 1349 595
Fragments arranged by size:
2400 1349 595 319 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 4674
Size: 212 4462
BbvCI CC'TCA_GC
Cuts at: 0 565 2609 3053 4674
Size: 565 2044 444 1621
Fragments arranged by size:
2044 1621 565 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2704 2738 3918 3952 4674
Size: 2704 34 1180 34 722
Fragments arranged by size:
2704 1180 722 34 34
BclI T'GATC_A
Cuts at: 0 465 4674
Size: 465 4209
BfrBI ATG'CAT
Cuts at: 0 2980 3374 4674
Size: 2980 394 1300
Fragments arranged by size:
2980 1300 394
BlpI GC'TnA_GC
Cuts at: 0 587 4674
Size: 587 4087
Bme1580I G_kGCm'C
Cuts at: 0 163 608 1234 1671 2755 4674
Size: 163 445 626 437 1084 1919
Fragments arranged by size:
1919 1084 626 445 437 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 2503 4294 4674
Size: 2503 1791 380
Fragments arranged by size:
2503 1791 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1551 1940 2050 3494 4455 4674
Size: 1551 389 110 1444 961 219
Fragments arranged by size:
1551 1444 961 389 219 110
Bpu10I CC'TnA_GC
Cuts at: 0 565 2609 3053 4674
Size: 565 2044 444 1621
Fragments arranged by size:
2044 1621 565 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 1956 2132 3263 3366 3585 3876 4615
Size: 1501 455 176 1131 103 219 291 739
Cuts at: 4615 4674
Size: 59
Fragments arranged by size:
1501 1131 739 455 291 219 176 103
59
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 1050 1905 2510 4674
Size: 109 941 855 605 2164
Fragments arranged by size:
2164 941 855 605 109
BsaAI yAC'GTr
Cuts at: 0 1672 4674
Size: 1672 3002
BsaBI GATnn'nnATC
Cuts at: 0 545 2696 4674
Size: 545 2151 1978
Fragments arranged by size:
2151 1978 545
BsaWI w'CCGG_w
Cuts at: 0 105 1413 4674
Size: 105 1308 3261
Fragments arranged by size:
3261 1308 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 82 1792 1819 2240 2626 3628 3656
Size: 20 62 1710 27 421 386 1002 28
Cuts at: 3656 3801 4624 4674
Size: 145 823 50
Fragments arranged by size:
1710 1002 823 421 386 145 62 50
28 27 20
BseYI C'CCAG_C
Cuts at: 0 256 1514 2747 4146 4674
Size: 256 1258 1233 1399 528
Fragments arranged by size:
1399 1258 1233 528 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1189 1768 4674
Size: 611 578 579 2906
Fragments arranged by size:
2906 611 579 578
BsiEI CG_ry'CG
Cuts at: 0 1695 4427 4674
Size: 1695 2732 247
Fragments arranged by size:
2732 1695 247
BsiHKAI G_wGCw'C
Cuts at: 0 490 1524 1537 2411 4079 4499 4674
Size: 490 1034 13 874 1668 420 175
Fragments arranged by size:
1668 1034 874 490 420 175 13
BsmI GAATG_Cn'
Cuts at: 0 357 2273 3376 4674
Size: 357 1916 1103 1298
Fragments arranged by size:
1916 1298 1103 357
Bsp1286I G_dGCh'C
Cuts at: 0 163 490 608 1234 1524 1537 1671 2400
Size: 163 327 118 626 290 13 134 729
Cuts at: 2400 2411 2730 2755 4079 4499 4674
Size: 11 319 25 1324 420 175
Fragments arranged by size:
1324 729 626 420 327 319 290 175
163 134 118 25 13 11
BspHI T'CATG_A
Cuts at: 0 1020 4674
Size: 1020 3654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 1047 1848 1923 2811 3318 3934 4674
Size: 633 414 801 75 888 507 616 740
Fragments arranged by size:
888 801 740 633 616 507 414 75
BsrDI GCAATG_nn'
Cuts at: 0 588 1108 2258 3012 3110 4193 4674
Size: 588 520 1150 754 98 1083 481
Fragments arranged by size:
1150 1083 754 588 520 481 98
BsrFI r'CCGG_y
Cuts at: 0 2940 4674
Size: 2940 1734
BsrGI T'GTAC_A
Cuts at: 0 480 3920 4094 4674
Size: 480 3440 174 580
Fragments arranged by size:
3440 580 480 174
BssSI C'ACGA_G
Cuts at: 0 4394 4674
Size: 4394 280
BstBI TT'CG_AA
Cuts at: 0 1835 4031 4674
Size: 1835 2196 643
Fragments arranged by size:
2196 1835 643
BstEII G'GTnAC_C
Cuts at: 0 3864 4674
Size: 3864 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4219 4674
Size: 4219 455
BstYI r'GATC_y
Cuts at: 0 434 514 546 1722 2076 2123 4674
Size: 434 80 32 1176 354 47 2551
Fragments arranged by size:
2551 1176 434 354 80 47 32
Bsu36I CC'TnA_GG
Cuts at: 0 2786 4674
Size: 2786 1888
BtgI C'CryG_G
Cuts at: 0 62 1817 3608 4413 4674
Size: 62 1755 1791 805 261
Fragments arranged by size:
1791 1755 805 261 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2138 2931 4130 4674
Size: 2138 793 1199 544
Fragments arranged by size:
2138 1199 793 544
BtsI GCAGTG_nn'
Cuts at: 0 979 1794 2358 4587 4674
Size: 979 815 564 2229 87
Fragments arranged by size:
2229 979 815 564 87
ClaI AT'CG_AT
Cuts at: 0 2690 3549 4674
Size: 2690 859 1125
Fragments arranged by size:
2690 1125 859
DraI TTT'AAA
Cuts at: 0 668 2165 4674
Size: 668 1497 2509
Fragments arranged by size:
2509 1497 668
EaeI y'GGCC_r
Cuts at: 0 1270 3378 3956 4424 4674
Size: 1270 2108 578 468 250
Fragments arranged by size:
2108 1270 578 468 250
EagI C'GGCC_G
Cuts at: 0 4424 4674
Size: 4424 250
EarI CTCTTCn'nnn_
Cuts at: 0 1124 2066 2416 2611 4127 4674
Size: 1124 942 350 195 1516 547
Fragments arranged by size:
1516 1124 942 547 350 195
EcoICRI GAG'CTC
Cuts at: 0 2409 4077 4674
Size: 2409 1668 597
Fragments arranged by size:
2409 1668 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1105 1551 1621 1831 1912 1940 1996
Size: 944 161 446 70 210 81 28 56
Cuts at: 1996 2050 2422 2989 3494 4138 4403 4455 4586
Size: 54 372 567 505 644 265 52 131
Cuts at: 4586 4674
Size: 88
Fragments arranged by size:
944 644 567 505 446 372 265 210
161 131 88 81 70 56 54 52
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1662 3410 4674
Size: 1662 1748 1264
Fragments arranged by size:
1748 1662 1264
EcoO109I rG'GnC_Cy
Cuts at: 0 1971 2472 3584 3724 4674
Size: 1971 501 1112 140 950
Fragments arranged by size:
1971 1112 950 501 140
EcoRI G'AATT_C
Cuts at: 0 2491 4674
Size: 2491 2183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2427 3885 4674
Size: 2427 1458 789
Fragments arranged by size:
2427 1458 789
FspI TGC'GCA
Cuts at: 0 2858 4674
Size: 2858 1816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 636 668 1438 1453 1470 1485
Size: 106 32 498 32 770 15 17 15
Cuts at: 1485 2914 2919 2946 2951 4674
Size: 1429 5 27 5 1723
Fragments arranged by size:
1723 1429 770 498 106 32 32 27
17 15 15 5 5
HincII GTy'rAC
Cuts at: 0 287 909 2802 3939 4674
Size: 287 622 1893 1137 735
Fragments arranged by size:
1893 1137 735 622 287
HindIII A'AGCT_T
Cuts at: 0 589 700 1477 2245 2879 2954 3201 4674
Size: 589 111 777 768 634 75 247 1473
Fragments arranged by size:
1473 777 768 634 589 247 111 75
KpnI G_GTAC'C
Cuts at: 0 248 1414 3463 3777 4674
Size: 248 1166 2049 314 897
Fragments arranged by size:
2049 1166 897 314 248
MfeI C'AATT_G
Cuts at: 0 3302 4674
Size: 3302 1372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1340 2384 4674
Size: 1056 284 1044 2290
Fragments arranged by size:
2290 1056 1044 284
MscI TGG'CCA
Cuts at: 0 1272 3380 3958 4674
Size: 1272 2108 578 716
Fragments arranged by size:
2108 1272 716 578
MslI CAynn'nnrTG
Cuts at: 0 2821 4102 4217 4418 4459 4490 4674
Size: 2821 1281 115 201 41 31 184
Fragments arranged by size:
2821 1281 201 184 115 41 31
MspA1I CmG'CkG
Cuts at: 0 13 1950 2647 2747 2967 4674
Size: 13 1937 697 100 220 1707
Fragments arranged by size:
1937 1707 697 220 100 13
NcoI C'CATG_G
Cuts at: 0 62 1817 4413 4674
Size: 62 1755 2596 261
Fragments arranged by size:
2596 1755 261 62
NsiI A_TGCA'T
Cuts at: 0 2982 3376 4674
Size: 2982 394 1298
Fragments arranged by size:
2982 1298 394
NspI r_CATG'y
Cuts at: 0 285 817 2820 3033 4165 4674
Size: 285 532 2003 213 1132 509
Fragments arranged by size:
2003 1132 532 509 285 213
PasI CC'CwG_GG
Cuts at: 0 4356 4674
Size: 4356 318
PciI A'CATG_T
Cuts at: 0 3029 4161 4674
Size: 3029 1132 513
Fragments arranged by size:
3029 1132 513
PfoI T'CCnGG_A
Cuts at: 0 987 1157 2522 4674
Size: 987 170 1365 2152
Fragments arranged by size:
2152 1365 987 170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1916 1948 4674
Size: 1916 32 2726
Fragments arranged by size:
2726 1916 32
PpuMI rG'GwC_Cy
Cuts at: 0 1971 2472 3584 3724 4674
Size: 1971 501 1112 140 950
Fragments arranged by size:
1971 1112 950 501 140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1993 2025 4674
Size: 1993 32 2649
Fragments arranged by size:
2649 1993 32
PstI C_TGCA'G
Cuts at: 0 2021 2652 4014 4674
Size: 2021 631 1362 660
Fragments arranged by size:
2021 1362 660 631
PvuI CG_AT'CG
Cuts at: 0 1695 4674
Size: 1695 2979
PvuII CAG'CTG
Cuts at: 0 1950 2647 2747 2967 4674
Size: 1950 697 100 220 1707
Fragments arranged by size:
1950 1707 697 220 100
SacI G_AGCT'C
Cuts at: 0 2411 4079 4674
Size: 2411 1668 595
Fragments arranged by size:
2411 1668 595
SalI G'TCGA_C
Cuts at: 0 907 3937 4674
Size: 907 3030 737
Fragments arranged by size:
3030 907 737
SapI GCTCTTCn'nnn_
Cuts at: 0 1124 2416 4674
Size: 1124 1292 2258
Fragments arranged by size:
2258 1292 1124
ScaI AGT'ACT
Cuts at: 0 2002 3091 4111 4674
Size: 2002 1089 1020 563
Fragments arranged by size:
2002 1089 1020 563
SfcI C'TryA_G
Cuts at: 0 576 614 2017 2134 2282 2648 4010 4674
Size: 576 38 1403 117 148 366 1362 664
Fragments arranged by size:
1403 1362 664 576 366 148 117 38
SmlI C'TyrA_G
Cuts at: 0 1480 1935 2147 3278 3345 3600 3855 4594
Size: 1480 455 212 1131 67 255 255 739
Cuts at: 4594 4674
Size: 80
Fragments arranged by size:
1480 1131 739 455 255 255 212 80
67
SpeI A'CTAG_T
Cuts at: 0 2335 2838 4674
Size: 2335 503 1836
Fragments arranged by size:
2335 1836 503
SphI G_CATG'C
Cuts at: 0 817 2820 4674
Size: 817 2003 1854
Fragments arranged by size:
2003 1854 817
SspI AAT'ATT
Cuts at: 0 4028 4674
Size: 4028 646
StuI AGG'CCT
Cuts at: 0 4479 4674
Size: 4479 195
StyI C'CwwG_G
Cuts at: 0 62 570 1405 1817 4413 4480 4674
Size: 62 508 835 412 2596 67 194
Fragments arranged by size:
2596 835 508 412 194 67 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 317 499 4674
Size: 133 184 182 4175
Fragments arranged by size:
4175 184 182 133
TatI w'GTAC_w
Cuts at: 0 480 1085 2000 3089 3920 4094 4109 4674
Size: 480 605 915 1089 831 174 15 565
Fragments arranged by size:
1089 915 831 605 565 480 174 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 4674
Size: 377 32 4265
Fragments arranged by size:
4265 377 32
Tth111I GACn'n_nGTC
Cuts at: 0 120 1559 4674
Size: 120 1439 3115
Fragments arranged by size:
3115 1439 120
XbaI T'CTAG_A
Cuts at: 0 201 4674
Size: 201 4473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1727 3018 4333 4613 4674
Size: 1727 1291 1315 280 61
Fragments arranged by size:
1727 1315 1291 280 61
XmnI GAAnn'nnTTC
Cuts at: 0 2248 3149 4027 4674
Size: 2248 901 878 647
Fragments arranged by size:
2248 901 878 647
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PfoI
PpiI PpuMI PsrI PstI PvuI PvuII SacI SalI
SapI ScaI SfcI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaI ApaLI
AscI AsiSI BbeI BciVI BglI BglII BmgBI BmtI
BplI BsaHI BsaXI BsiWI BsmBI BspEI BsrBI BssHII
BstAPI BstZ17I CspCI DraIII DrdI EciI EcoRV FseI
FspAI HaeII HpaI KasI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI RsrII SacII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SwaI XhoI XmaI ZraI