Restriction map for
pFN21AA0166
(Linear) (Six-base) MAPSORT of: KIAA0166.seq Check: 7356 from: 1 to: 6627
KIAA0166.orf
With 182 enzymes: SgfI *
December 5, 2014 12:52 ..
AccI GT'mk_AC
Cuts at: 0 536 1364 3697 4909 6627
Size: 536 828 2333 1212 1718
Fragments arranged by size:
2333 1718 1212 828 536
Acc65I G'GTAC_C
Cuts at: 0 47 445 3838 6627
Size: 47 398 3393 2789
Fragments arranged by size:
3393 2789 398 47
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 484 525 956 1157 1250 1871 2783
Size: 275 209 41 431 201 93 621 912
Cuts at: 2783 3573 3760 3769 3832 4369 4960 4975 5117
Size: 790 187 9 63 537 591 15 142
Cuts at: 5117 5653 6024 6512 6622 6627
Size: 536 371 488 110 5
Fragments arranged by size:
912 790 621 591 537 536 488 431
371 275 209 201 187 142 110 93
63 41 15 9 5
AflII C'TTAA_G
Cuts at: 0 162 2887 4186 6510 6627
Size: 162 2725 1299 2324 117
Fragments arranged by size:
2725 2324 1299 162 117
AflIII A'CryG_T
Cuts at: 0 3316 4734 4904 4920 5450 6627
Size: 3316 1418 170 16 530 1177
Fragments arranged by size:
3316 1418 1177 530 170 16
AgeI A'CCGG_T
Cuts at: 0 673 6627
Size: 673 5954
AleI CACnn'nnGTG
Cuts at: 0 2155 6627
Size: 2155 4472
AlwNI CAG_nnn'CTG
Cuts at: 0 1150 2466 3168 3367 5248 6583 6627
Size: 1150 1316 702 199 1881 1335 44
Fragments arranged by size:
1881 1335 1316 1150 702 199 44
ApaLI G'TGCA_C
Cuts at: 0 1688 6627
Size: 1688 4939
ApoI r'AATT_y
Cuts at: 0 512 1405 1597 1621 3756 4691 4817 5125
Size: 512 893 192 24 2135 935 126 308
Cuts at: 5125 5354 5361 6279 6596 6627
Size: 229 7 918 317 31
Fragments arranged by size:
2135 935 918 893 512 317 308 229
192 126 31 24 7
AseI AT'TA_AT
Cuts at: 0 955 4640 4646 4876 5429 6627
Size: 955 3685 6 230 553 1198
Fragments arranged by size:
3685 1198 955 553 230 6
AvaI C'yCGr_G
Cuts at: 0 5660 6627
Size: 5660 967
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 39 72 242 275 6627
Size: 39 33 170 33 6352
Fragments arranged by size:
6352 170 39 33 33
BamHI G'GATC_C
Cuts at: 0 4566 6627
Size: 4566 2061
BanI G'GyrC_C
Cuts at: 0 47 445 3838 6627
Size: 47 398 3393 2789
Fragments arranged by size:
3393 2789 398 47
BanII G_rGCy'C
Cuts at: 0 1554 3113 3136 4147 5667 6627
Size: 1554 1559 23 1011 1520 960
Fragments arranged by size:
1559 1554 1520 1011 960 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 2732 2875 3838 6627
Size: 1145 1587 143 963 2789
Fragments arranged by size:
2789 1587 1145 963 143
BbvCI CC'TCA_GC
Cuts at: 0 3122 6627
Size: 3122 3505
BciVI GTATCCnnnnn_n'
Cuts at: 0 6022 6627
Size: 6022 605
BclI T'GATC_A
Cuts at: 0 2084 6105 6627
Size: 2084 4021 522
Fragments arranged by size:
4021 2084 522
BfrBI ATG'CAT
Cuts at: 0 367 5634 6456 6627
Size: 367 5267 822 171
Fragments arranged by size:
5267 822 367 171
BglII A'GATC_T
Cuts at: 0 113 2678 3039 4020 6627
Size: 113 2565 361 981 2607
Fragments arranged by size:
2607 2565 981 361 113
Bme1580I G_kGCm'C
Cuts at: 0 1692 2802 5242 6627
Size: 1692 1110 2440 1385
Fragments arranged by size:
2440 1692 1385 1110
BmrI ACTGGGnnnn_n'
Cuts at: 0 3305 3349 4209 6627
Size: 3305 44 860 2418
Fragments arranged by size:
3305 2418 860 44
BmtI G_CTAG'C
Cuts at: 0 2975 3811 6352 6627
Size: 2975 836 2541 275
Fragments arranged by size:
2975 2541 836 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1771 2871 3217 5991 6560 6627
Size: 653 1118 1100 346 2774 569 67
Fragments arranged by size:
2774 1118 1100 653 569 346 67
Bpu10I CC'TnA_GC
Cuts at: 0 1373 2053 3122 6627
Size: 1373 680 1069 3505
Fragments arranged by size:
3505 1373 1069 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1442 2386 3234 3631 4516 6627
Size: 1442 944 848 397 885 2111
Fragments arranged by size:
2111 1442 944 885 848 397
BsaAI yAC'GTr
Cuts at: 0 879 929 4905 6627
Size: 879 50 3976 1722
Fragments arranged by size:
3976 1722 879 50
BsaBI GATnn'nnATC
Cuts at: 0 392 1992 6627
Size: 392 1600 4635
Fragments arranged by size:
4635 1600 392
BsaWI w'CCGG_w
Cuts at: 0 37 673 6627
Size: 37 636 5954
Fragments arranged by size:
5954 636 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 834 864 6627
Size: 834 30 5763
Fragments arranged by size:
5763 834 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2269 3144 4688 5545 6627
Size: 2269 875 1544 857 1082
Fragments arranged by size:
2269 1544 1082 875 857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2456 3139 3976 4029 5566 6627
Size: 2456 683 837 53 1537 1061
Fragments arranged by size:
2456 1537 1061 837 683 53
BsiEI CG_ry'CG
Cuts at: 0 5236 6627
Size: 5236 1391
BsiHKAI G_wGCw'C
Cuts at: 0 1554 1692 2402 3103 4522 5255 5667 6627
Size: 1554 138 710 701 1419 733 412 960
Fragments arranged by size:
1554 1419 960 733 710 701 412 138
BsmI GAATG_Cn'
Cuts at: 0 685 3879 4223 5594 6627
Size: 685 3194 344 1371 1033
Fragments arranged by size:
3194 1371 1033 685 344
Bsp1286I G_dGCh'C
Cuts at: 0 1554 1692 2402 2802 3103 3113 3136 4147
Size: 1554 138 710 400 301 10 23 1011
Cuts at: 4147 4522 5242 5255 5667 6627
Size: 375 720 13 412 960
Fragments arranged by size:
1554 1011 960 720 710 412 400 375
301 138 23 13 10
BspHI T'CATG_A
Cuts at: 0 3527 3869 6627
Size: 3527 342 2758
Fragments arranged by size:
3527 2758 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5933 6018 6627
Size: 5933 85 609
Fragments arranged by size:
5933 609 85
BsrDI GCAATG_nn'
Cuts at: 0 3291 4855 6627
Size: 3291 1564 1772
Fragments arranged by size:
3291 1772 1564
BsrFI r'CCGG_y
Cuts at: 0 673 4423 5228 6627
Size: 673 3750 805 1399
Fragments arranged by size:
3750 1399 805 673
BsrGI T'GTAC_A
Cuts at: 0 2273 6627
Size: 2273 4354
BssSI C'ACGA_G
Cuts at: 0 5866 6627
Size: 5866 761
BstBI TT'CG_AA
Cuts at: 0 4870 4928 6627
Size: 4870 58 1699
Fragments arranged by size:
4870 1699 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4482 6627
Size: 4482 2145
BstYI r'GATC_y
Cuts at: 0 113 1391 2678 3039 3555 4020 4566 4707
Size: 113 1278 1287 361 516 465 546 141
Cuts at: 4707 6627
Size: 1920
Fragments arranged by size:
1920 1287 1278 546 516 465 361 141
113
Bsu36I CC'TnA_GG
Cuts at: 0 6302 6627
Size: 6302 325
BtgI C'CryG_G
Cuts at: 0 1804 2348 2876 4703 5873 6627
Size: 1804 544 528 1827 1170 754
Fragments arranged by size:
1827 1804 1170 754 544 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4388 5153 6120 6475 6627
Size: 4388 765 967 355 152
Fragments arranged by size:
4388 967 765 355 152
BtsI GCAGTG_nn'
Cuts at: 0 2426 3279 6073 6555 6581 6627
Size: 2426 853 2794 482 26 46
Fragments arranged by size:
2794 2426 853 482 46 26
DraI TTT'AAA
Cuts at: 0 509 1812 2642 2961 5718 5996 6314 6627
Size: 509 1303 830 319 2757 278 318 313
Fragments arranged by size:
2757 1303 830 509 319 318 313 278
DraIII CAC_nnn'GTG
Cuts at: 0 3858 6627
Size: 3858 2769
EaeI y'GGCC_r
Cuts at: 0 1924 4425 4484 6627
Size: 1924 2501 59 2143
Fragments arranged by size:
2501 2143 1924 59
EarI CTCTTCn'nnn_
Cuts at: 0 123 878 2813 3560 4396 5535 5672 5683
Size: 123 755 1935 747 836 1139 137 11
Cuts at: 5683 6320 6627
Size: 637 307
Fragments arranged by size:
1935 1139 836 755 747 637 307 137
123 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 414 6627
Size: 414 6213
EcoICRI GAG'CTC
Cuts at: 0 1552 5665 6627
Size: 1552 4113 962
Fragments arranged by size:
4113 1552 962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 484 525 653 956 1157 1250 1771
Size: 275 209 41 128 303 201 93 521
Cuts at: 1771 1871 2783 2871 3217 3573 3760 3769 3832
Size: 100 912 88 346 356 187 9 63
Cuts at: 3832 4369 4960 4975 5117 5653 5991 6024 6512
Size: 537 591 15 142 536 338 33 488
Cuts at: 6512 6560 6622 6627
Size: 48 62 5
Fragments arranged by size:
912 591 537 536 521 488 356 346
338 303 275 209 201 187 142 128
100 93 88 63 62 48 41 33
15 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5083 5828 6627
Size: 5083 745 799
Fragments arranged by size:
5083 799 745
EcoRI G'AATT_C
Cuts at: 0 5354 5361 6627
Size: 5354 7 1266
Fragments arranged by size:
5354 1266 7
EcoRV GAT'ATC
Cuts at: 0 3685 5091 5114 6627
Size: 3685 1406 23 1513
Fragments arranged by size:
3685 1513 1406 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 907 1660 1696 6627
Size: 907 753 36 4931
Fragments arranged by size:
4931 907 753 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 774 806 1132 1164 2069 2101 2588 2605
Size: 774 32 326 32 905 32 487 17
Cuts at: 2605 2620 2637 2670 2702 3384 3416 3639 3671
Size: 15 17 33 32 682 32 223 32
Cuts at: 3671 5829 5861 5964 5996 6097 6129 6627
Size: 2158 32 103 32 101 32 498
Fragments arranged by size:
2158 905 774 682 498 487 326 223
103 101 33 32 32 32 32 32
32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 0 4961 5046 5642 6627
Size: 4961 85 596 985
Fragments arranged by size:
4961 985 596 85
HindIII A'AGCT_T
Cuts at: 0 1178 3325 3533 5212 5953 6524 6627
Size: 1178 2147 208 1679 741 571 103
Fragments arranged by size:
2147 1679 1178 741 571 208 103
HpaI GTT'AAC
Cuts at: 0 4961 5642 6627
Size: 4961 681 985
Fragments arranged by size:
4961 985 681
KpnI G_GTAC'C
Cuts at: 0 51 449 3842 6627
Size: 51 398 3393 2785
Fragments arranged by size:
3393 2785 398 51
MfeI C'AATT_G
Cuts at: 0 232 524 1942 5570 6319 6627
Size: 232 292 1418 3628 749 308
Fragments arranged by size:
3628 1418 749 308 292 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1859 4346 4709 5438 6627
Size: 1859 2487 363 729 1189
Fragments arranged by size:
2487 1859 1189 729 363
MscI TGG'CCA
Cuts at: 0 1926 4486 6627
Size: 1926 2560 2141
Fragments arranged by size:
2560 2141 1926
MslI CAynn'nnrTG
Cuts at: 0 2155 3284 6627
Size: 2155 1129 3343
Fragments arranged by size:
3343 2155 1129
MspA1I CmG'CkG
Cuts at: 0 780 2440 4756 6111 6627
Size: 780 1660 2316 1355 516
Fragments arranged by size:
2316 1660 1355 780 516
NaeI GCC'GGC
Cuts at: 0 4425 6627
Size: 4425 2202
NcoI C'CATG_G
Cuts at: 0 1804 2348 5873 6627
Size: 1804 544 3525 754
Fragments arranged by size:
3525 1804 754 544
NdeI CA'TA_TG
Cuts at: 0 2794 3561 5802 6332 6627
Size: 2794 767 2241 530 295
Fragments arranged by size:
2794 2241 767 530 295
NgoMIV G'CCGG_C
Cuts at: 0 4423 6627
Size: 4423 2204
NheI G'CTAG_C
Cuts at: 0 2971 3807 6348 6627
Size: 2971 836 2541 279
Fragments arranged by size:
2971 2541 836 279
NsiI A_TGCA'T
Cuts at: 0 369 5636 6458 6627
Size: 369 5267 822 169
Fragments arranged by size:
5267 822 369 169
NspI r_CATG'y
Cuts at: 0 158 3320 3931 4738 5454 6460 6627
Size: 158 3162 611 807 716 1006 167
Fragments arranged by size:
3162 1006 807 716 611 167 158
PciI A'CATG_T
Cuts at: 0 3316 4734 5450 6627
Size: 3316 1418 716 1177
Fragments arranged by size:
3316 1418 1177 716
PflMI CCAn_nnn'nTGG
Cuts at: 0 1933 6627
Size: 1933 4694
PfoI T'CCnGG_A
Cuts at: 0 1394 6627
Size: 1394 5233
PmlI CAC'GTG
Cuts at: 0 929 6627
Size: 929 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 853 885 5649 5681 6627
Size: 45 32 776 32 4764 32 946
Fragments arranged by size:
4764 946 776 45 32 32 32
PsiI TTA'TAA
Cuts at: 0 2221 3754 6627
Size: 2221 1533 2873
Fragments arranged by size:
2873 2221 1533
PspXI vC'TCGA_Gb
Cuts at: 0 5660 6627
Size: 5660 967
PstI C_TGCA'G
Cuts at: 0 2781 2924 3179 5930 6627
Size: 2781 143 255 2751 697
Fragments arranged by size:
2781 2751 697 255 143
PvuI CG_AT'CG
Cuts at: 0 5236 6627
Size: 5236 1391
PvuII CAG'CTG
Cuts at: 0 780 2440 4756 6111 6627
Size: 780 1660 2316 1355 516
Fragments arranged by size:
2316 1660 1355 780 516
SacI G_AGCT'C
Cuts at: 0 1554 5667 6627
Size: 1554 4113 960
Fragments arranged by size:
4113 1554 960
SapI GCTCTTCn'nnn_
Cuts at: 0 2813 4396 5672 6320 6627
Size: 2813 1583 1276 648 307
Fragments arranged by size:
2813 1583 1276 648 307
ScaI AGT'ACT
Cuts at: 0 1160 4206 6627
Size: 1160 3046 2421
Fragments arranged by size:
3046 2421 1160
SexAI A'CCwGG_T
Cuts at: 0 5487 6627
Size: 5487 1140
SfcI C'TryA_G
Cuts at: 0 899 2682 2777 2861 2920 3175 3482 4826
Size: 899 1783 95 84 59 255 307 1344
Cuts at: 4826 4910 5039 5060 5155 5702 5926 6627
Size: 84 129 21 95 547 224 701
Fragments arranged by size:
1783 1344 899 701 547 307 255 224
129 95 95 84 84 59 21
SmlI C'TyrA_G
Cuts at: 0 162 1457 2401 2887 3213 3610 4186 4495
Size: 162 1295 944 486 326 397 576 309
Cuts at: 4495 5660 6510 6627
Size: 1165 850 117
Fragments arranged by size:
1295 1165 944 850 576 486 397 326
309 162 117
SphI G_CATG'C
Cuts at: 0 158 6460 6627
Size: 158 6302 167
Fragments arranged by size:
6302 167 158
SspI AAT'ATT
Cuts at: 0 4348 4638 4644 5284 6547 6627
Size: 4348 290 6 640 1263 80
Fragments arranged by size:
4348 1263 640 290 80 6
StuI AGG'CCT
Cuts at: 0 130 2848 6627
Size: 130 2718 3779
Fragments arranged by size:
3779 2718 130
StyI C'CwwG_G
Cuts at: 0 1804 2348 2428 3689 4428 5873 6627
Size: 1804 544 80 1261 739 1445 754
Fragments arranged by size:
1804 1445 1261 754 739 544 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1360 6627
Size: 1360 5267
TatI w'GTAC_w
Cuts at: 0 544 1158 2102 2273 4204 6627
Size: 544 614 944 171 1931 2423
Fragments arranged by size:
2423 1931 944 614 544 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 834 866 5364 5396 6627
Size: 834 32 4498 32 1231
Fragments arranged by size:
4498 1231 834 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1144 6627
Size: 1144 5483
XbaI T'CTAG_A
Cuts at: 0 4710 6627
Size: 4710 1917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3192 3566 6627
Size: 3192 374 3061
Fragments arranged by size:
3192 3061 374
XhoI C'TCGA_G
Cuts at: 0 5660 6627
Size: 5660 967
XmnI GAAnn'nnTTC
Cuts at: 0 887 4692 6627
Size: 887 3805 1935
Fragments arranged by size:
3805 1935 887
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmlI PpiI PsiI PspXI PstI PvuI
PvuII SacI SapI ScaI SexAI SfcI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AhdI AlfI AloI ApaI
AscI AsiSI AvrII BbeI BcgI BglI BlpI BmgBI
BplI BsaI BsaHI BseYI BsiWI BsmBI BspEI BsrBI
BssHII BstAPI BstEII BstZ17I ClaI CspCI DrdI EagI
EcoO109I FseI FspI FspAI HaeII KasI MluI NarI
NotI NruI PacI PasI PmeI PpuMI PshAI PspOMI
PsrI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XmaI
ZraI