Restriction map for
pFN21AA0042
(Linear) (Six-base) MAPSORT of: KIAA0042.seq Check: 2084 from: 1 to: 4944
KIAA0042.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 701 931 3527 4300 4629 4772 4944
Size: 253 448 230 2596 773 329 143 172
Fragments arranged by size:
2596 773 448 329 253 230 172 143
AfeI AGC'GCT
Cuts at: 0 1825 4944
Size: 1825 3119
AflII C'TTAA_G
Cuts at: 0 106 634 886 2250 4944
Size: 106 528 252 1364 2694
Fragments arranged by size:
2694 1364 528 252 106
AflIII A'CryG_T
Cuts at: 0 349 1089 4944
Size: 349 740 3855
Fragments arranged by size:
3855 740 349
AleI CACnn'nnGTG
Cuts at: 0 1726 4944
Size: 1726 3218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3197 4944
Size: 3197 1747
AlwNI CAG_nnn'CTG
Cuts at: 0 385 2228 4944
Size: 385 1843 2716
Fragments arranged by size:
2716 1843 385
ApoI r'AATT_y
Cuts at: 0 805 1502 1750 2004 2559 2773 3146 3197
Size: 805 697 248 254 555 214 373 51
Cuts at: 3197 3593 3636 3693 3719 3818 4234 4504 4944
Size: 396 43 57 26 99 416 270 440
Fragments arranged by size:
805 697 555 440 416 396 373 270
254 248 214 99 57 51 43 26
AseI AT'TA_AT
Cuts at: 0 1877 3803 4944
Size: 1877 1926 1141
Fragments arranged by size:
1926 1877 1141
BanII G_rGCy'C
Cuts at: 0 895 3259 4883 4909 4944
Size: 895 2364 1624 26 35
Fragments arranged by size:
2364 1624 895 35 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 329 1134 3128 4944
Size: 329 805 1994 1816
Fragments arranged by size:
1994 1816 805 329
BbvCI CC'TCA_GC
Cuts at: 0 881 4944
Size: 881 4063
BclI T'GATC_A
Cuts at: 0 2541 4944
Size: 2541 2403
BglII A'GATC_T
Cuts at: 0 163 1416 1806 4393 4944
Size: 163 1253 390 2587 551
Fragments arranged by size:
2587 1253 551 390 163
BlpI GC'TnA_GC
Cuts at: 0 2894 4944
Size: 2894 2050
BmgBI CAC'GTC
Cuts at: 0 352 4944
Size: 352 4592
BmrI ACTGGGnnnn_n'
Cuts at: 0 602 2581 4051 4919 4944
Size: 602 1979 1470 868 25
Fragments arranged by size:
1979 1470 868 602 25
BmtI G_CTAG'C
Cuts at: 0 1256 1282 4944
Size: 1256 26 3662
Fragments arranged by size:
3662 1256 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 914 1845 4944
Size: 914 931 3099
Fragments arranged by size:
3099 931 914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3432 4484 4576 4944
Size: 3432 1052 92 368
Fragments arranged by size:
3432 1052 368 92
Bpu10I CC'TnA_GC
Cuts at: 0 881 4266 4944
Size: 881 3385 678
Fragments arranged by size:
3385 881 678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1412 2484 4030 4944
Size: 627 785 1072 1546 914
Fragments arranged by size:
1546 1072 914 785 627
BsaBI GATnn'nnATC
Cuts at: 0 548 4583 4944
Size: 548 4035 361
Fragments arranged by size:
4035 548 361
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3558 3588 4944
Size: 3558 30 1356
Fragments arranged by size:
3558 1356 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 3579 4723 4944
Size: 288 3291 1144 221
Fragments arranged by size:
3291 1144 288 221
BseYI C'CCAG_C
Cuts at: 0 894 4896 4944
Size: 894 4002 48
Fragments arranged by size:
4002 894 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1898 3262 4541 4944
Size: 891 1007 1364 1279 403
Fragments arranged by size:
1364 1279 1007 891 403
BsiHKAI G_wGCw'C
Cuts at: 0 3259 4500 4883 4909 4944
Size: 3259 1241 383 26 35
Fragments arranged by size:
3259 1241 383 35 26
BsmI GAATG_Cn'
Cuts at: 0 87 3642 4122 4921 4944
Size: 87 3555 480 799 23
Fragments arranged by size:
3555 799 480 87 23
Bsp1286I G_dGCh'C
Cuts at: 0 895 3259 4500 4883 4909 4944
Size: 895 2364 1241 383 26 35
Fragments arranged by size:
2364 1241 895 383 35 26
BspHI T'CATG_A
Cuts at: 0 1145 3852 4944
Size: 1145 2707 1092
Fragments arranged by size:
2707 1145 1092
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 160 4944
Size: 160 4784
BsrDI GCAATG_nn'
Cuts at: 0 659 2024 2108 3452 4521 4944
Size: 659 1365 84 1344 1069 423
Fragments arranged by size:
1365 1344 1069 659 423 84
BsrGI T'GTAC_A
Cuts at: 0 841 4131 4339 4944
Size: 841 3290 208 605
Fragments arranged by size:
3290 841 605 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2023 2167 4944
Size: 2023 144 2777
Fragments arranged by size:
2777 2023 144
BstBI TT'CG_AA
Cuts at: 0 1307 4944
Size: 1307 3637
BstEII G'GTnAC_C
Cuts at: 0 4353 4944
Size: 4353 591
BstYI r'GATC_y
Cuts at: 0 163 549 982 1416 1806 2424 4393 4944
Size: 163 386 433 434 390 618 1969 551
Fragments arranged by size:
1969 618 551 434 433 390 386 163
BtgI C'CryG_G
Cuts at: 0 4379 4884 4944
Size: 4379 505 60
Fragments arranged by size:
4379 505 60
BtsI GCAGTG_nn'
Cuts at: 0 1824 4342 4907 4944
Size: 1824 2518 565 37
Fragments arranged by size:
2518 1824 565 37
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2284 2319 4944
Size: 2284 35 2625
Fragments arranged by size:
2625 2284 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3619 4944
Size: 3619 1325
EaeI y'GGCC_r
Cuts at: 0 4622 4944
Size: 4622 322
EarI CTCTTCn'nnn_
Cuts at: 0 534 2730 2960 4944
Size: 534 2196 230 1984
Fragments arranged by size:
2196 1984 534 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3124 4944
Size: 3124 1820
EcoICRI GAG'CTC
Cuts at: 0 3257 4881 4907 4944
Size: 3257 1624 26 37
Fragments arranged by size:
3257 1624 37 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 701 931 3432 3527 4300 4484 4576
Size: 253 448 230 2501 95 773 184 92
Cuts at: 4576 4629 4772 4944
Size: 53 143 172
Fragments arranged by size:
2501 773 448 253 230 184 172 143
95 92 53
EcoRI G'AATT_C
Cuts at: 0 3146 4234 4504 4944
Size: 3146 1088 270 440
Fragments arranged by size:
3146 1088 440 270
EcoRV GAT'ATC
Cuts at: 0 735 1632 4944
Size: 735 897 3312
Fragments arranged by size:
3312 897 735
HaeII r_GCGC'y
Cuts at: 0 1827 4944
Size: 1827 3117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1554 1586 2181 2210 2213 2242 4075 4107
Size: 1554 32 595 29 3 29 1833 32
Cuts at: 4107 4380 4412 4476 4508 4944
Size: 273 32 64 32 436
Fragments arranged by size:
1833 1554 595 436 273 64 32 32
32 32 29 29 3
HincII GTy'rAC
Cuts at: 0 2106 3072 4944
Size: 2106 966 1872
Fragments arranged by size:
2106 1872 966
HindIII A'AGCT_T
Cuts at: 0 3155 4944
Size: 3155 1789
MluI A'CGCG_T
Cuts at: 0 1089 4944
Size: 1089 3855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 299 710 2179 2460 4944
Size: 104 195 411 1469 281 2484
Fragments arranged by size:
2484 1469 411 281 195 104
MscI TGG'CCA
Cuts at: 0 4624 4944
Size: 4624 320
MslI CAynn'nnrTG
Cuts at: 0 565 1471 1726 1844 4944
Size: 565 906 255 118 3100
Fragments arranged by size:
3100 906 565 255 118
MspA1I CmG'CkG
Cuts at: 0 35 406 2041 4944
Size: 35 371 1635 2903
Fragments arranged by size:
2903 1635 371 35
NcoI C'CATG_G
Cuts at: 0 4379 4884 4944
Size: 4379 505 60
Fragments arranged by size:
4379 505 60
NdeI CA'TA_TG
Cuts at: 0 1587 2607 2925 4944
Size: 1587 1020 318 2019
Fragments arranged by size:
2019 1587 1020 318
NheI G'CTAG_C
Cuts at: 0 1252 1278 4944
Size: 1252 26 3666
Fragments arranged by size:
3666 1252 26
NspI r_CATG'y
Cuts at: 0 234 3935 4944
Size: 234 3701 1009
Fragments arranged by size:
3701 1009 234
PasI CC'CwG_GG
Cuts at: 0 4778 4944
Size: 4778 166
PfoI T'CCnGG_A
Cuts at: 0 3714 4226 4944
Size: 3714 512 718
Fragments arranged by size:
3714 718 512
PshAI GACnn'nnGTC
Cuts at: 0 1264 4944
Size: 1264 3680
PsiI TTA'TAA
Cuts at: 0 1196 3824 4790 4944
Size: 1196 2628 966 154
Fragments arranged by size:
2628 1196 966 154
PstI C_TGCA'G
Cuts at: 0 171 390 1041 4944
Size: 171 219 651 3903
Fragments arranged by size:
3903 651 219 171
PvuII CAG'CTG
Cuts at: 0 406 4944
Size: 406 4538
SacI G_AGCT'C
Cuts at: 0 3259 4883 4909 4944
Size: 3259 1624 26 35
Fragments arranged by size:
3259 1624 35 26
ScaI AGT'ACT
Cuts at: 0 15 640 4944
Size: 15 625 4304
Fragments arranged by size:
4304 625 15
SfcI C'TryA_G
Cuts at: 0 167 251 386 1019 1037 2474 3202 3785
Size: 167 84 135 633 18 1437 728 583
Cuts at: 3785 4944
Size: 1159
Fragments arranged by size:
1437 1159 728 633 583 167 135 84
18
SmlI C'TyrA_G
Cuts at: 0 106 634 642 886 1427 2250 2499 4009
Size: 106 528 8 244 541 823 249 1510
Cuts at: 4009 4944
Size: 935
Fragments arranged by size:
1510 935 823 541 528 249 244 106
8
SspI AAT'ATT
Cuts at: 0 2205 4944
Size: 2205 2739
StyI C'CwwG_G
Cuts at: 0 3845 4379 4884 4944
Size: 3845 534 505 60
Fragments arranged by size:
3845 534 505 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 672 1987 4929 4938 4944
Size: 106 566 1315 2942 9 6
Fragments arranged by size:
2942 1315 566 106 9 6
TatI w'GTAC_w
Cuts at: 0 13 638 841 2548 3192 4131 4339 4446
Size: 13 625 203 1707 644 939 208 107
Cuts at: 4446 4944
Size: 498
Fragments arranged by size:
1707 939 644 625 498 208 203 107
13
XbaI T'CTAG_A
Cuts at: 0 3556 4194 4944
Size: 3556 638 750
Fragments arranged by size:
3556 750 638
XmnI GAAnn'nnTTC
Cuts at: 0 1502 1967 3594 4610 4944
Size: 1502 465 1627 1016 334
Fragments arranged by size:
1627 1502 1016 465 334
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI AlfI AlwNI ApoI
AseI BanII BbsI BbvCI BclI BglII BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstAPI BstBI BstEII BstYI BtgI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV HaeII Hin4I HincII HindIII MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NspI PasI PfoI
PshAI PsiI PstI PvuII SacI ScaI SfcI SmlI
SspI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BfrBI BglI Bme1580I BsaI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EagI EcoNI EcoO109I FalI FseI FspI
FspAI HpaI KasI KpnI MfeI NaeI NarI NgoMIV
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XcmI XhoI XmaI ZraI