Restriction map for
pF1KE2583
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp718I BamHI BbrPI BbvCI BcgI
BfrI BsaWI BseAI BsiWI Bsp1407I
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DraIII EcoO65I
EcoT22I FseI KpnI KspI MluI
MroI NaeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1537 GTCGAC GT^[AC][GT]AC
AhdI 976 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1032 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3913 AGCGCT AGC^GCT
AseI 3193 ATTAAT AT^TAAT
AsnI 3193 ATTAAT AT^TAAT
AspEI 976 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 990 TGCGCA TGC^GCA
AvrII 4786 CCTAGG C^CTAGG
BlnI 4786 CCTAGG C^CTAGG
BsaI 868 GGTCTC GGTCTC(1/5)
BsgI 3604 GTGCAG GTGCAG(16/14)
BsiEI 3492 CGGCCG CG[GA][CT]^CG
BsmBI 3484 CGTCTC CGTCTC(1/5)
BspDI 3935 ATCGAT AT^CGAT
Bsu36I 1032 CCTCAGG CC^T[ACGT]AGG
ClaI 3935 ATCGAT AT^CGAT
DraI 4257 TTTAAA TTT^AAA
DrdI 3424 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3492 CGGCCG C^GGCCG
Eam1105I 976 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3492 CGGCCG C^GGCCG
Eco47III 3913 AGCGCT AGC^GCT
Eco52I 3492 CGGCCG C^GGCCG
Eco81I 1032 CCTCAGG CC^T[ACGT]AGG
FspI 990 TGCGCA TGC^GCA
HgaI 3483 GCGTC GACGC(5/10)
HpaI 4471 GTTAAC GTT^AAC
MfeI 4510 CAATTG C^AATTG
MunI 4510 CAATTG C^AATTG
NdeI 2420 CATATG CA^TATG
NotI 3491 GCGGCCGC GC^GGCCGC
PshAI 3999 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3193 ATTAAT AT^TAAT
SalI 1537 GTCGAC G^TCGAC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 778 GGCGCC G[GA]^CG[CT]C
3259 GGCGCC
ApaI 885 GGGCCC GGGCC^C
1300 GGGCCC
AspI 687 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACTGGGTC
AvaI 3857 CCCGGG C^[CT]CG[GA]G
4445 CCCGGG
BbeI 778 GGCGCC GGCGC^C
3259 GGCGCC
BclI 488 TGATCA T^GATCA
3449 TGATCA
BglI 1302 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2041 GCCCTGGTGGC
BsaAI 556 CACGTA [CT]AC^GT[GA]
2980 TACGTG
BsaHI 778 GGCGCC G[GA]^CG[CT]C
3259 GGCGCC
BsoBI 3857 CCCGGG C^[CT]CG[GA]G
4445 CCCGGG
BsrDI 2286 CATTGC GCAATG(2/0)
4527 GCAATG
EcoNI 486 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3840 CCTGATCCAGG
EcoRI 295 GAATTC G^AATTC
2581 GAATTC
FauI 15 GCGGG CCCGC(4/6)
3820 GCGGG
FbaI 488 TGATCA T^GATCA
3449 TGATCA
Hin1I 778 GGCGCC G[GA]^CG[CT]C
3259 GGCGCC
HindIII 4431 AAGCTT A^AGCTT
4904 AAGCTT
KasI 778 GGCGCC G^GCGCC
3259 GGCGCC
NarI 778 GGCGCC GG^CGCC
3259 GGCGCC
PflFI 687 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACTGGGTC
SacI 3 GAGCTC GAGCT^C
30 GAGCTC
SapI 925 GAAGAGC GCTCTTC(1/4)
3549 GCTCTTC
ScaI 998 AGTACT AGT^ACT
2807 AGTACT
SexAI 653 ACCTGGT A^CC[AT]GGT
3377 ACCAGGT
SfiI 1301 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2040 GGCCCTGGTGGCC
SfoI 778 GGCGCC GGC^GCC
3259 GGCGCC
SmaI 3857 CCCGGG CCC^GGG
4445 CCCGGG
SmlI 25 CTCAAG C^T[CT][GA]AG
2511 CTCAAG
Tth111I 687 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1625 GACTGGGTC
XmaCI 3857 CCCGGG C^CCGGG
4445 CCCGGG
XmaI 3857 CCCGGG C^CCGGG
4445 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI