Restriction map for
pF1KE2673
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanII BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BpmI BsaAI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BstBI BstUI Bsu36I
Cfr10I CpoI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT14I
FbaI FseI FspI KpnI KspI
MluI MroI MslI MspA1I MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 37 GGCGCC G[GA]^CG[CT]C
AlwNI 985 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 21 GAGCAC G[AT]GC[AT]^C
BamHI 693 GGATCC G^GATCC
BbeI 37 GGCGCC GGCGC^C
BglII 2298 AGATCT A^GATCT
BlpI 2004 GCTTAGC GC^T[ACGT]AGC
BmyI 21 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 2004 GCTTAGC GC^T[ACGT]AGC
BsaBI 643 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BsiHKAI 21 GAGCAC G[AT]GC[AT]^C
BsmBI 1534 CGTCTC CGTCTC(1/5)
BsmFI 1590 GGGAC GGGAC(10/14)
Bsp1286I 21 GAGCAC G[AGT]GC[ACT]^C
BspDI 650 ATCGAT AT^CGAT
BspMI 1768 ACCTGC ACCTGC(4/8)
BssSI 836 CTCGTG CACGAG(-5/-1)
Bst1107I 1548 GTATAC GTA^TAC
BstAPI 1402 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1021 GGTTACC G^GT[ACGT]ACC
BstNI 629 CCAGG CC^[AT]GG
BstPI 1021 GGTTACC G^GT[ACGT]ACC
BstZ17I 1548 GTATAC GTA^TAC
CelII 2004 GCTTAGC GC^T[ACGT]AGC
ClaI 650 ATCGAT AT^CGAT
DraIII 585 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1021 GGTTACC G^GT[ACGT]ACC
EcoRII 629 CCAGG ^CC[AT]GG
EcoT22I 1469 ATGCAT ATGCA^T
FauI 111 GCGGG CCCGC(4/6)
HgaI 1028 GACGC GACGC(5/10)
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HincII 681 GTTAAC GT[CT]^[GA]AC
HindII 681 GTTAAC GT[CT]^[GA]AC
HpaI 681 GTTAAC GTT^AAC
KasI 37 GGCGCC G^GCGCC
MamI 643 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1655 CAATTG C^AATTG
MunI 1655 CAATTG C^AATTG
MvaI 629 CCAGG CC^[AT]GG
NarI 37 GGCGCC GG^CGCC
NsiI 1469 ATGCAT ATGCA^T
NspI 1806 GCATGT [GA]CATG^[CT]
PaeR7I 1988 CTCGAG C^TCGAG
PshAI 1010 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 37 GGCGCC GGC^GCC
SmlI 1988 CTCGAG C^T[CT][GA]AG
XhoI 1988 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1219 GTCTAC GT^[AC][GT]AC
1548 GTATAC
AciI 7 GCGG CCGC(-3/-1)
111 GCGG
AlwI 694 GATCC GGATC(4/5)
693 GGATC
AvaII 27 GGACC G^G[AT]CC
1570 GGTCC
BalI 659 TGGCCA TGG^CCA
1081 TGGCCA
BanI 37 GGCGCC G^G[CT][GA]CC
1765 GGCACC
BbsI 1342 GAAGAC GAAGAC(2/6)
1966 GAAGAC
BcnI 785 CCCGG CC^[GC]GG
827 CCCGG
BpuAI 1342 GAAGAC GAAGAC(2/6)
1966 GAAGAC
BsiYI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1413 CCTCAAAGTGG
BslI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1413 CCTCAAAGTGG
BstXI 564 CCATGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1913 CCAAACTGCTGG
BstYI 693 GGATCC [GA]^GATC[CT]
2298 AGATCT
DraI 729 TTTAAA TTT^AAA
2148 TTTAAA
EaeI 659 TGGCCA [CT]^GGCC[GA]
1081 TGGCCA
EarI 72 CTCTTC CTCTTC(1/4)
2175 GAAGAG
HaeII 37 GGCGCC [GA]GCGC^[CT]
96 GGCGCT
HindIII 145 AAGCTT A^AGCTT
1941 AAGCTT
Ksp632I 72 CTCTTC CTCTTC(1/4)
2175 GAAGAG
MaeII 865 ACGT A^CGT
2029 ACGT
MflI 693 GGATCC [GA]^GATC[CT]
2298 AGATCT
MluNI 659 TGGCCA TGG^CCA
1081 TGGCCA
MscI 659 TGGCCA TGG^CCA
1081 TGGCCA
NciI 785 CCCGG CC^[GC]GG
827 CCCGG
PleI 355 GACTC GAGTC(4/5)
1322 GACTC
TspRI 996 CACTG CA[GC]TG[ACGT][ACGT]^
1484 CACTG
XhoII 693 GGATCC [GA]^GATC[CT]
2298 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AvaI BbvI BfaI BseRI BsmAI
BsmI BsoBI BspHI BsrDI BsrI
BssKI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII EcoRI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MwoI NdeII NlaIII NlaIV PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TthHB8I XcmI
XmnI