Restriction map for
pF1KE2709
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# NO CUT ENZYMES ( 50 enzymes) #
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AccI AclI AflII AgeI Alw44I
ApaLI AseI AsnI AvrII BbeI
BciVI BfrI BglI BlnI BsaBI
BspDI BsrDI Bst1107I BstBI BstZ17I
ClaI EcoRV FseI HpaI KasI
MamI MfeI MluI MunI NarI
NdeI NheI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI SalI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 2543 TCCGGA T^CCGGA
AscI 3728 GGCGCGCC GG^CGCGCC
AspI 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3950 TGCGCA TGC^GCA
BamHI 2506 GGATCC G^GATCC
BbvCI 149 CCTCAGC CCTCAGC(-5/-2)
BglII 3131 AGATCT A^GATCT
BsaWI 2543 TCCGGA [AT]^CCGG[AT]
BseAI 2543 TCCGGA T^CCGGA
Bsp1407I 2303 TGTACA T^GTACA
BspEI 2543 TCCGGA T^CCGGA
BspHI 3323 TCATGA T^CATGA
BsrGI 2303 TGTACA T^GTACA
BssHII 3729 GCGCGC G^CGCGC
CpoI 4771 CGGTCCG CG^G[AT]CCG
DraI 3361 TTTAAA TTT^AAA
EcoT22I 235 ATGCAT ATGCA^T
FspI 3950 TGCGCA TGC^GCA
KspI 1072 CCGCGG CCGC^GG
MroI 2543 TCCGGA T^CCGGA
NotI 4332 GCGGCCGC GC^GGCCGC
NruI 4468 TCGCGA TCG^CGA
NsiI 235 ATGCAT ATGCA^T
PaeR7I 1927 CTCGAG C^TCGAG
PflFI 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2641 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3323 TCATGA T^CATGA
RsrII 4771 CGGTCCG CG^G[AT]CCG
SacII 1072 CCGCGG CCGC^GG
SapI 2011 GAAGAGC GCTCTTC(1/4)
SspBI 2303 TGTACA T^GTACA
Tth111I 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1927 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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Acc65I 188 GGTACC G^GTACC
1610 GGTACC
AflIII 2996 ACATGT A^C[GA][CT]GT
3149 ACATGT
AhdI 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3703 GACCAGAAGTC
Aor51HI 1284 AGCGCT AGC^GCT
2238 AGCGCT
Asp718I 188 GGTACC G^GTACC
1610 GGTACC
AspEI 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3703 GACCAGAAGTC
BalI 569 TGGCCA TGG^CCA
1511 TGGCCA
BbrPI 1861 CACGTG CAC^GTG
2174 CACGTG
BclI 3512 TGATCA T^GATCA
4406 TGATCA
BlpI 3459 GCTCAGC GC^T[ACGT]AGC
4440 GCTCAGC
Bpu1102I 3459 GCTCAGC GC^T[ACGT]AGC
4440 GCTCAGC
BsaI 1822 GAGACC GGTCTC(1/5)
3898 GAGACC
BsiWI 2456 CGTACG C^GTACG
2576 CGTACG
BsmBI 2091 CGTCTC CGTCTC(1/5)
2899 CGTCTC
BspLU11I 2996 ACATGT A^CATGT
3149 ACATGT
BstEII 355 GGTTACC G^GT[ACGT]ACC
2647 GGTCACC
BstPI 355 GGTTACC G^GT[ACGT]ACC
2647 GGTCACC
CelII 3459 GCTCAGC GC^T[ACGT]AGC
4440 GCTCAGC
DrdI 3895 GACGAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4645 GACTCCATCGTC
Eam1105I 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3703 GACCAGAAGTC
Eco47III 1284 AGCGCT AGC^GCT
2238 AGCGCT
EcoO65I 355 GGTTACC G^GT[ACGT]ACC
2647 GGTCACC
EcoRI 1142 GAATTC G^AATTC
3260 GAATTC
FbaI 3512 TGATCA T^GATCA
4406 TGATCA
HindIII 2749 AAGCTT A^AGCTT
4792 AAGCTT
KpnI 188 GGTACC GGTAC^C
1610 GGTACC
MluNI 569 TGGCCA TGG^CCA
1511 TGGCCA
MscI 569 TGGCCA TGG^CCA
1511 TGGCCA
PmaCI 1861 CACGTG CAC^GTG
2174 CACGTG
PmlI 1861 CACGTG CAC^GTG
2174 CACGTG
SbfI 1098 CCTGCAGG CCTGCA^GG
3738 CCTGCAGG
ScaI 3443 AGTACT AGT^ACT
4487 AGTACT
Sse8387I 1098 CCTGCAGG CCTGCA^GG
3738 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI