Restriction map for
pF1KE2708
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI BalI BbeI BbrPI BsaAI
BsaI BseAI BsgI BsiWI Bsp1407I
BspEI BspMI BsrGI BssHII BstBI
BstEII BstPI CpoI DraI Eco47III
EcoO65I EcoRI EcoRV HindIII KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NheI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 4027 CTTAAG C^TTAAG
AflIII 2906 ACATGT A^C[GA][CT]GT
AhdI 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3143 CCTCAGG CC^T[ACGT]AGG
AspEI 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1199 TGCGCA TGC^GCA
BbvCI 1696 GCTGAGG CCTCAGC(-5/-2)
BcgI 537 GCATCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1070 GGATAC GTATCC(6/5)
BfrI 4027 CTTAAG C^TTAAG
BglI 3284 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4057 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3233 TCATGA T^CATGA
BspLU11I 2906 ACATGT A^CATGT
BssSI 3631 CACGAG CACGAG(-5/-1)
Bst1107I 236 GTATAC GTA^TAC
BstXI 1430 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 236 GTATAC GTA^TAC
Bsu36I 3143 CCTCAGG CC^T[ACGT]AGG
Eam1105I 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3143 CCTCAGG CC^T[ACGT]AGG
FseI 853 GGCCGGCC GGCCGG^CC
FspI 1199 TGCGCA TGC^GCA
HincII 769 GTTAAC GT[CT]^[GA]AC
HindII 769 GTTAAC GT[CT]^[GA]AC
HpaI 769 GTTAAC GTT^AAC
KspI 893 CCGCGG CCGC^GG
MamI 4057 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshAI 2589 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3233 TCATGA T^CATGA
SacII 893 CCGCGG CCGC^GG
SbfI 3648 CCTGCAGG CCTGCA^GG
SmlI 4027 CTTAAG C^T[CT][GA]AG
Sse8387I 3648 CCTGCAGG CCTGCA^GG
SspI 3906 AATATT AAT^ATT
StuI 1005 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 236 GTATAC GT^[AC][GT]AC
2810 GTCTAC
AseI 2158 ATTAAT AT^TAAT
4323 ATTAAT
AsnI 2158 ATTAAT AT^TAAT
4323 ATTAAT
AvrII 3096 CCTAGG C^CTAGG
4389 CCTAGG
BglII 1894 AGATCT A^GATCT
4460 AGATCT
BlnI 3096 CCTAGG C^CTAGG
4389 CCTAGG
BsmBI 399 CGTCTC CGTCTC(1/5)
1219 GAGACG
BspDI 229 ATCGAT AT^CGAT
4171 ATCGAT
BsrDI 2277 CATTGC GCAATG(2/0)
4547 GCAATG
BstAPI 210 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
825 GCAGAAGCTGC
ClaI 229 ATCGAT AT^CGAT
4171 ATCGAT
DraIII 2608 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
3199 CACCGAGTG
EcoT22I 208 ATGCAT ATGCA^T
217 ATGCAT
HaeII 1256 AGCGCC [GA]GCGC^[CT]
3929 AGCGCC
NdeI 2366 CATATG CA^TATG
2938 CATATG
NotI 895 GCGGCCGC GC^GGCCGC
3556 GCGGCCGC
NsiI 208 ATGCAT ATGCA^T
217 ATGCAT
PshBI 2158 ATTAAT AT^TAAT
4323 ATTAAT
SacI 548 GAGCTC GAGCT^C
3476 GAGCTC
XcmI 1121 CCAAAGCAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2448 CCAGCTACGGAGTGG
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# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI