Restriction map for
pF1KE2692
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrGI Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SalI SbfI SexAI SfcI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 355 GGTACC G^GTACC
AscI 18 GGCGCGCC GG^CGCGCC
Asp718I 355 GGTACC G^GTACC
AvaI 30 CTCGGG C^[CT]CG[GA]G
BbeI 48 GGCGCC GGCGC^C
BciVI 586 GGATAC GTATCC(6/5)
BclI 1815 TGATCA T^GATCA
BglI 54 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1766 ACCGGA [AT]^CCGG[AT]
BsiEI 107 CGGCCG CG[GA][CT]^CG
BsmBI 1081 GAGACG CGTCTC(1/5)
BsoBI 30 CTCGGG C^[CT]CG[GA]G
BspHI 707 TCATGA T^CATGA
BsrBI 1387 CCGCTC CCGCTC(-3/-3)
BsrDI 1474 GCAATG GCAATG(2/0)
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BssHII 19 GCGCGC G^CGCGC
BstAPI 1507 GCATCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 451 TTCGAA TT^CGAA
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
DraII 409 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1844 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 107 CGGCCG C^GGCCG
EclXI 107 CGGCCG C^GGCCG
Eco52I 107 CGGCCG C^GGCCG
EcoO109I 409 AGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 1815 TGATCA T^GATCA
HincII 1684 GTTGAC GT[CT]^[GA]AC
HindII 1684 GTTGAC GT[CT]^[GA]AC
KasI 48 GGCGCC G^GCGCC
KpnI 355 GGTACC GGTAC^C
MslI 1446 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 48 GGCGCC GG^CGCC
NotI 106 GCGGCCGC GC^GGCCGC
NspI 822 GCATGC [GA]CATG^[CT]
NspV 451 TTCGAA TT^CGAA
PvuII 390 CAGCTG CAG^CTG
RcaI 707 TCATGA T^CATGA
SacI 166 GAGCTC GAGCT^C
ScaI 1667 AGTACT AGT^ACT
SfoI 48 GGCGCC GGC^GCC
SfuI 451 TTCGAA TT^CGAA
SmlI 2028 CTCAAG C^T[CT][GA]AG
SphI 822 GCATGC GCATG^C
StuI 1461 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 736 AAATTT [GA]^AATT[CT]
1434 GAATTT
AcyI 48 GGCGCC G[GA]^CG[CT]C
1621 GGCGTC
AocI 345 CCTGAGG CC^T[ACGT]AGG
1464 CCTCAGG
ApoI 736 AAATTT [GA]^AATT[CT]
1434 GAATTT
AspHI 166 GAGCTC G[AT]GC[AT]^C
1110 GAGCAC
AvaII 1644 GGACC G^G[AT]CC
1852 GGTCC
BalI 311 TGGCCA TGG^CCA
1490 TGGCCA
BbsI 403 GAAGAC GAAGAC(2/6)
1951 GAAGAC
BpuAI 403 GAAGAC GAAGAC(2/6)
1951 GAAGAC
BsaHI 48 GGCGCC G[GA]^CG[CT]C
1621 GGCGTC
BsiHKAI 166 GAGCTC G[AT]GC[AT]^C
1110 GAGCAC
BssSI 298 CACGAG CACGAG(-5/-1)
2049 CACGAG
BstXI 1355 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2048 CCACGAGTCTGG
BstYI 625 GGATCT [GA]^GATC[CT]
834 GGATCT
Bsu36I 345 CCTGAGG CC^T[ACGT]AGG
1464 CCTCAGG
Eco81I 345 CCTGAGG CC^T[ACGT]AGG
1464 CCTCAGG
EcoT14I 158 CCATGG C^C[AT][AT]GG
1007 CCATGG
Hin1I 48 GGCGCC G[GA]^CG[CT]C
1621 GGCGTC
KspI 58 CCGCGG CCGC^GG
110 CCGCGG
MflI 625 GGATCT [GA]^GATC[CT]
834 GGATCT
MluNI 311 TGGCCA TGG^CCA
1490 TGGCCA
MscI 311 TGGCCA TGG^CCA
1490 TGGCCA
NcoI 158 CCATGG C^CATGG
1007 CCATGG
SacII 58 CCGCGG CCGC^GG
110 CCGCGG
SapI 2076 GAAGAGC GCTCTTC(1/4)
267 GCTCTTC
StyI 158 CCATGG C^C[AT][AT]GG
1007 CCATGG
XhoII 625 GGATCT [GA]^GATC[CT]
834 GGATCT
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I