Restriction map for
pF1KE2631
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsiWI
BsmI BspDI BspHI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MaeI MluI NaeI NdeI
NgoMIV NheI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspI
StuI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 1333 GTCTAC GT^[AC][GT]AC
AclI 2428 AACGTT AA^CGTT
AflIII 1706 ACATGT A^C[GA][CT]GT
Aor51HI 2456 AGCGCT AGC^GCT
Asp700I 2214 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1468 GGATCC G^GATCC
BbvCI 1743 GCTGAGG CCTCAGC(-5/-2)
BciVI 1931 GGATAC GTATCC(6/5)
BsaI 509 GGTCTC GGTCTC(1/5)
Bsp1407I 581 TGTACA T^GTACA
BspLU11I 1706 ACATGT A^CATGT
BsrFI 270 GCCGGT [GA]^CCGG[CT]
BsrGI 581 TGTACA T^GTACA
Cfr10I 270 GCCGGT [GA]^CCGG[CT]
Eco47III 2456 AGCGCT AGC^GCT
HincII 754 GTCAAC GT[CT]^[GA]AC
HindII 754 GTCAAC GT[CT]^[GA]AC
HindIII 3202 AAGCTT A^AGCTT
KspI 3312 CCGCGG CCGC^GG
MfeI 455 CAATTG C^AATTG
MseI 2761 TTAA T^TAA
MunI 455 CAATTG C^AATTG
NotI 3320 GCGGCCGC GC^GGCCGC
Psp1406I 2428 AACGTT AA^CGTT
SacI 1077 GAGCTC GAGCT^C
SacII 3312 CCGCGG CCGC^GG
SbfI 1563 CCTGCAGG CCTGCA^GG
Sse8387I 1563 CCTGCAGG CCTGCA^GG
SspBI 581 TGTACA T^GTACA
Tru9I 2761 TTAA T^TAA
XmnI 2214 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AccIII 2822 TCCGGA T^CCGGA
3000 TCCGGA
Alw44I 2059 GTGCAC G^TGCAC
2494 GTGCAC
AlwNI 1735 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2017 CAGCTGCTG
AocI 1269 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
ApaLI 2059 GTGCAC G^TGCAC
2494 GTGCAC
BbeI 2189 GGCGCC GGCGC^C
2336 GGCGCC
BcgI 2925 CGATCAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1145 GCACTCCTATCG
BglI 907 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2164 GCCCAGCGGGC
BsaBI 1638 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2565 GATGCGTATC
BsaWI 2822 TCCGGA [AT]^CCGG[AT]
3000 TCCGGA
BseAI 2822 TCCGGA T^CCGGA
3000 TCCGGA
BsiEI 3155 CGGCCG CG[GA][CT]^CG
3321 CGGCCG
BsmBI 931 GAGACG CGTCTC(1/5)
2872 GAGACG
BspEI 2822 TCCGGA T^CCGGA
3000 TCCGGA
BssSI 2810 CACGAG CACGAG(-5/-1)
1420 CTCGTG
BstAPI 646 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1734 GCAGACGCTGC
Bsu36I 1269 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
EagI 3155 CGGCCG C^GGCCG
3321 CGGCCG
EclXI 3155 CGGCCG C^GGCCG
3321 CGGCCG
Eco52I 3155 CGGCCG C^GGCCG
3321 CGGCCG
Eco81I 1269 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
EcoT22I 2677 ATGCAT ATGCA^T
2755 ATGCAT
KasI 2189 GGCGCC G^GCGCC
2336 GGCGCC
MamI 1638 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2565 GATGCGTATC
MroI 2822 TCCGGA T^CCGGA
3000 TCCGGA
NarI 2189 GGCGCC GG^CGCC
2336 GGCGCC
NsiI 2677 ATGCAT ATGCA^T
2755 ATGCAT
PaeR7I 1539 CTCGAG C^TCGAG
3271 CTCGAG
PpuMI 1265 GGGACCT [GA]G^G[AT]CC[CT]
3275 AGGTCCT
SfoI 2189 GGCGCC GGC^GCC
2336 GGCGCC
SmaI 48 CCCGGG CCC^GGG
3191 CCCGGG
SphI 2507 GCATGC GCATG^C
3146 GCATGC
TfiI 1716 GAATC G^A[AT]TC
2832 GAATC
Tsp509I 456 AATT ^AATT
1458 AATT
XcmI 578 CCATGTACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1029 CCAGGGCATCCCTGG
XhoI 1539 CTCGAG C^TCGAG
3271 CTCGAG
XmaCI 48 CCCGGG C^CCGGG
3191 CCCGGG
XmaI 48 CCCGGG C^CCGGG
3191 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII