Restriction map for
pF1KE2580
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KspI MamI MluI MroI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 2015 GGTACC G^GTACC
AflIII 1772 ACATGT A^C[GA][CT]GT
Asp718I 2015 GGTACC G^GTACC
BglI 793 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1448 CTGGAG CTGGAG(16/14)
BsaI 3116 GAGACC GGTCTC(1/5)
BsiEI 205 CGGTCG CG[GA][CT]^CG
BsmI 181 GAATGC GAATGC(1/-1)
BspLU11I 1772 ACATGT A^CATGT
BssSI 3172 CACGAG CACGAG(-5/-1)
BstBI 2402 TTCGAA TT^CGAA
DraII 1610 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 2296 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2690 CCACGG C^C[GA][CT]GG
EcoO109I 1610 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1871 CCAAGG C^C[AT][AT]GG
HindIII 1424 AAGCTT A^AGCTT
KpnI 2015 GGTACC GGTAC^C
NdeI 2465 CATATG CA^TATG
NspV 2402 TTCGAA TT^CGAA
PshAI 1570 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2674 GAGCTC GAGCT^C
SalI 2916 GTCGAC G^TCGAC
SfuI 2402 TTCGAA TT^CGAA
SpeI 2575 ACTAGT A^CTAGT
StyI 1871 CCAAGG C^C[AT][AT]GG
XbaI 3429 TCTAGA T^CTAGA
XcmI 2690 CCACGGCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AccI 715 GTAGAC GT^[AC][GT]AC
2916 GTCGAC
AlwNI 3007 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
3187 CAGCAGCTG
AspHI 2674 GAGCTC G[AT]GC[AT]^C
3230 GAGCAC
BalI 2 TGGCCA TGG^CCA
2346 TGGCCA
BamHI 225 GGATCC G^GATCC
2354 GGATCC
BanI 2015 GGTACC G^G[CT][GA]CC
2155 GGCACC
BanII 598 GAGCCC G[GA]GC[CT]^C
2674 GAGCTC
BbvCI 3126 CCTCAGC CCTCAGC(-5/-2)
3097 GCTGAGG
BclI 1703 TGATCA T^GATCA
3080 TGATCA
BsaAI 2161 TACGTA [CT]AC^GT[GA]
2201 TACGTA
BsiHKAI 2674 GAGCTC G[AT]GC[AT]^C
3230 GAGCAC
BsmFI 3201 GTCCC GGGAC(10/14)
3483 GTCCC
Bsp1407I 1034 TGTACA T^GTACA
1138 TGTACA
BspHI 2036 TCATGA T^CATGA
2922 TCATGA
BspMI 3119 ACCTGC ACCTGC(4/8)
2115 GCAGGT
BsrGI 1034 TGTACA T^GTACA
1138 TGTACA
BstXI 230 CCAAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3389 CCAAAGAAATGG
DraI 2793 TTTAAA TTT^AAA
2823 TTTAAA
EaeI 2 TGGCCA [CT]^GGCC[GA]
2346 TGGCCA
EcoNI 1149 CCTTTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2934 CCTAGAGAAGG
FbaI 1703 TGATCA T^GATCA
3080 TGATCA
HapII 2293 CCGG C^CGG
2381 CCGG
HgaI 3009 GACGC GACGC(5/10)
1601 GCGTC
HincII 2881 GTTGAC GT[CT]^[GA]AC
2916 GTCGAC
HindII 2881 GTTGAC GT[CT]^[GA]AC
2916 GTCGAC
HpaII 2293 CCGG C^CGG
2381 CCGG
MfeI 1823 CAATTG C^AATTG
1975 CAATTG
MluNI 2 TGGCCA TGG^CCA
2346 TGGCCA
MscI 2 TGGCCA TGG^CCA
2346 TGGCCA
MslI 813 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2223 CATGGTTATG
MspA1I 1417 CAGCTG C[AC]G^C[GT]G
3190 CAGCTG
MspI 2293 CCGG C^CGG
2381 CCGG
MunI 1823 CAATTG C^AATTG
1975 CAATTG
PflMI 936 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCATCCTCTGG
PstI 300 CTGCAG CTGCA^G
1233 CTGCAG
PvuII 1417 CAGCTG CAG^CTG
3190 CAGCTG
RcaI 2036 TCATGA T^CATGA
2922 TCATGA
SnaBI 2161 TACGTA TAC^GTA
2201 TACGTA
SspBI 1034 TGTACA T^GTACA
1138 TGTACA
StuI 381 AGGCCT AGG^CCT
852 AGGCCT
Van91I 936 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCATCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BcgI
BfaI BglII BmyI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRI EcoRII EcoRV
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII