Restriction map for
pF1KB4407
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I AvrII BbrPI BlnI
BsiWI BspDI BspLU11I BssSI Bst1107I
BstBI BstZ17I ClaI DrdI EcoRV
FseI HpaI KpnI KspI MfeI
MluI MunI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
SacII SalI SbfI SfiI SfuI
SnaBI SpeI Sse8387I XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 3098 GACGTC GACGT^C
AccI 801 GTCTAC GT^[AC][GT]AC
AccIII 1137 TCCGGA T^CCGGA
AflII 382 CTTAAG C^TTAAG
Alw44I 2612 GTGCAC G^TGCAC
Aor51HI 5066 AGCGCT AGC^GCT
ApaLI 2612 GTGCAC G^TGCAC
AspI 3137 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2727 GGATCC G^GATCC
BbeI 4986 GGCGCC GGCGC^C
BcgI 699 GCAGGAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5167 GTATCC GTATCC(6/5)
BfrI 382 CTTAAG C^TTAAG
BglI 3991 GCCATACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1483 AGATCT A^GATCT
BsaAI 1363 TACGTG [CT]AC^GT[GA]
BseAI 1137 TCCGGA T^CCGGA
Bsp1407I 336 TGTACA T^GTACA
BspEI 1137 TCCGGA T^CCGGA
BspHI 3179 TCATGA T^CATGA
BsrGI 336 TGTACA T^GTACA
BssHII 4963 GCGCGC G^CGCGC
CpoI 4589 CGGACCG CG^G[AT]CCG
DraIII 4540 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco47III 5066 AGCGCT AGC^GCT
Eco52I 12 CGGCCG C^GGCCG
EcoT22I 2033 ATGCAT ATGCA^T
HincII 1951 GTCAAC GT[CT]^[GA]AC
HindII 1951 GTCAAC GT[CT]^[GA]AC
KasI 4986 GGCGCC G^GCGCC
MroI 1137 TCCGGA T^CCGGA
NaeI 4850 GCCGGC GCC^GGC
NarI 4986 GGCGCC GG^CGCC
NgoMIV 4850 GCCGGC G^CCGGC
NsiI 2033 ATGCAT ATGCA^T
PflFI 3137 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 4415 CGATCG CGAT^CG
RcaI 3179 TCATGA T^CATGA
RsrII 4589 CGGACCG CG^G[AT]CCG
ScaI 190 AGTACT AGT^ACT
SexAI 2871 ACCAGGT A^CC[AT]GGT
SfoI 4986 GGCGCC GGC^GCC
SgrAI 4849 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 890 CCCGGG CCC^GGG
SphI 1873 GCATGC GCATG^C
SspBI 336 TGTACA T^GTACA
StuI 1497 AGGCCT AGG^CCT
SwaI 2130 ATTTAAAT ATTT^AAAT
Tth111I 3137 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 890 CCCGGG C^CCGGG
XmaI 890 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 225 CGCG CG^CG
4964 CGCG
AflIII 2863 ACACGT A^C[GA][CT]GT
3766 ACACGT
ApaI 1512 GGGCCC GGGCC^C
5108 GGGCCC
BanI 3226 GGTGCC G^G[CT][GA]CC
4986 GGCGCC
BbsI 4822 GAAGAC GAAGAC(2/6)
2150 GTCTTC
BlpI 2944 GCTCAGC GC^T[ACGT]AGC
4554 GCTGAGC
Bpu1102I 2944 GCTCAGC GC^T[ACGT]AGC
4554 GCTGAGC
BpuAI 4822 GAAGAC GAAGAC(2/6)
2150 GTCTTC
BsaWI 1137 TCCGGA [AT]^CCGG[AT]
4587 ACCGGA
BsmBI 4014 CGTCTC CGTCTC(1/5)
4880 GAGACG
BsrDI 524 CATTGC GCAATG(2/0)
1941 CATTGC
BstEII 2358 GGTGACC G^GT[ACGT]ACC
3933 GGTCACC
BstPI 2358 GGTGACC G^GT[ACGT]ACC
3933 GGTCACC
BstUI 225 CGCG CG^CG
4964 CGCG
CelII 2944 GCTCAGC GC^T[ACGT]AGC
4554 GCTGAGC
DraI 765 TTTAAA TTT^AAA
2131 TTTAAA
EcoO65I 2358 GGTGACC G^GT[ACGT]ACC
3933 GGTCACC
EcoRI 1283 GAATTC G^AATTC
1768 GAATTC
MvnI 225 CGCG CG^CG
4964 CGCG
PflMI 2758 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3237 CCACGGTCTGG
SspI 775 AATATT AAT^ATT
1999 AATATT
Van91I 2758 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3237 CCACGGTCTGG
XbaI 1186 TCTAGA T^CTAGA
2187 TCTAGA
XcmI 1061 CCAAGAAACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4508 CCAAGGAGAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
Asp700I AspEI AspHI AvaI AvaII
AviII BalI BanII BbvCI BbvI
BclI BcnI BfaI BmyI BpmI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI