Restriction map for
pF1KE2655
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AviII AvrII
BamHI BanI BbeI BbvCI BciVI
BclI BfrI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BssHII BssSI Bst1107I BstAPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI Ksp632I
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AhdI 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 832 ATTAAT AT^TAAT
AsnI 832 ATTAAT AT^TAAT
AspEI 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 361 CACGTG CAC^GTG
BcgI 1290 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1282 CTAG C^TAG
BlpI 4 GCTGAGC GC^T[ACGT]AGC
BpmI 476 CTCCAG CTGGAG(16/14)
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BsaAI 361 CACGTG [CT]AC^GT[GA]
BseRI 1233 CTCCTC GAGGAG(10/8)
BsgI 823 GTGCAG GTGCAG(16/14)
BsiWI 50 CGTACG C^GTACG
BsmBI 1208 CGTCTC CGTCTC(1/5)
Bsp1407I 1178 TGTACA T^GTACA
BsrDI 1226 GCAATG GCAATG(2/0)
BsrFI 1240 ACCGGC [GA]^CCGG[CT]
BsrGI 1178 TGTACA T^GTACA
BstBI 22 TTCGAA TT^CGAA
BstEII 951 GGTGACC G^GT[ACGT]ACC
BstPI 951 GGTGACC G^GT[ACGT]ACC
CelII 4 GCTGAGC GC^T[ACGT]AGC
CfoI 271 GCGC GCG^C
Cfr10I 1240 ACCGGC [GA]^CCGG[CT]
DraII 1063 GGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 1288 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1063 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 951 GGTGACC G^GT[ACGT]ACC
EcoT14I 425 CCAAGG C^C[AT][AT]GG
HgaI 118 GACGC GACGC(5/10)
HhaI 271 GCGC GCG^C
HinP1I 271 GCGC G^CGC
HincII 937 GTTGAC GT[CT]^[GA]AC
HindII 937 GTTGAC GT[CT]^[GA]AC
HindIII 1027 AAGCTT A^AGCTT
KspI 460 CCGCGG CCGC^GG
MaeI 1282 CTAG C^TAG
MseI 833 TTAA T^TAA
MslI 1248 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1281 GCTAGC G^CTAGC
NspV 22 TTCGAA TT^CGAA
PflFI 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 994 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 361 CACGTG CAC^GTG
PmlI 361 CACGTG CAC^GTG
PpuMI 1063 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 832 ATTAAT AT^TAAT
PvuII 305 CAGCTG CAG^CTG
SacI 248 GAGCTC GAGCT^C
SacII 460 CCGCGG CCGC^GG
SfiI 481 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 22 TTCGAA TT^CGAA
SspBI 1178 TGTACA T^GTACA
StyI 425 CCAAGG C^C[AT][AT]GG
Tru9I 833 TTAA T^TAA
Tsp509I 831 AATT ^AATT
Tth111I 1042 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 994 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 925 GTCTAC GT^[AC][GT]AC
1258 GTAGAC
AflIII 894 ACACGT A^C[GA][CT]GT
1205 ACACGT
AspHI 201 GAGCAC G[AT]GC[AT]^C
248 GAGCTC
AvaI 659 CCCGGG C^[CT]CG[GA]G
900 CCCGGG
AvaII 684 GGTCC G^G[AT]CC
1064 GGTCC
BalI 1002 TGGCCA TGG^CCA
1307 TGGCCA
BbsI 343 GAAGAC GAAGAC(2/6)
708 GTCTTC
BpuAI 343 GAAGAC GAAGAC(2/6)
708 GTCTTC
BsiHKAI 201 GAGCAC G[AT]GC[AT]^C
248 GAGCTC
BsoBI 659 CCCGGG C^[CT]CG[GA]G
900 CCCGGG
BsrBI 530 CCGCTC CCGCTC(-3/-3)
624 CCGCTC
BstF5I 981 CATCC GGATG(2/0)
240 GGATG
BstXI 668 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1083 CCACGTCCCTGG
EaeI 1002 TGGCCA [CT]^GGCC[GA]
1307 TGGCCA
EcoNI 942 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1090 CCTGGCGGAGG
FokI 981 CATCC GGATG(9/13)
240 GGATG
MluNI 1002 TGGCCA TGG^CCA
1307 TGGCCA
MscI 1002 TGGCCA TGG^CCA
1307 TGGCCA
PleI 1261 GACTC GAGTC(4/5)
1046 GAGTC
PstI 142 CTGCAG CTGCA^G
727 CTGCAG
SmaI 659 CCCGGG CCC^GGG
900 CCCGGG
TfiI 133 GAATC G^A[AT]TC
510 GATTC
XcmI 861 CCAGTCCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1050 CCAGTCGAAGATTGG
XmaCI 659 CCCGGG C^CCGGG
900 CCCGGG
XmaI 659 CCCGGG C^CCGGG
900 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanII BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I XhoII