Restriction map for
pF1KE2589
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AvrII BamHI BclI BfrI BlnI
BsaI BsiWI BspHI BsrFI BssHII
Bst1107I BstBI BstXI BstZ17I Cfr10I
CpoI DraI DraIII Eam1105I EcoRI
EcoRV FbaI FseI HincII HindII
HpaI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1367 GGTACC G^GTACC
AccIII 2177 TCCGGA T^CCGGA
AcyI 1625 GGCGCC G[GA]^CG[CT]C
AflIII 2462 ACATGT A^C[GA][CT]GT
Alw44I 313 GTGCAC G^TGCAC
Aor51HI 2880 AGCGCT AGC^GCT
ApaLI 313 GTGCAC G^TGCAC
Asp700I 2460 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1367 GGTACC G^GTACC
AspI 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2288 TGCGCA TGC^GCA
BbeI 1625 GGCGCC GGCGC^C
BbvCI 1876 GCTGAGG CCTCAGC(-5/-2)
BcgI 1820 GCAAATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1230 GGATAC GTATCC(6/5)
BglI 2260 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2265 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1625 GGCGCC G[GA]^CG[CT]C
BseAI 2177 TCCGGA T^CCGGA
BsiEI 4 CGGCCG CG[GA][CT]^CG
BsmBI 792 CGTCTC CGTCTC(1/5)
Bsp1407I 1343 TGTACA T^GTACA
BspDI 556 ATCGAT AT^CGAT
BspEI 2177 TCCGGA T^CCGGA
BspLU11I 2462 ACATGT A^CATGT
BsrGI 1343 TGTACA T^GTACA
BstEII 1692 GGTCACC G^GT[ACGT]ACC
BstPI 1692 GGTCACC G^GT[ACGT]ACC
ClaI 556 ATCGAT AT^CGAT
DrdI 484 GACGCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4 CGGCCG C^GGCCG
EclXI 4 CGGCCG C^GGCCG
Eco47III 2880 AGCGCT AGC^GCT
Eco52I 4 CGGCCG C^GGCCG
EcoO65I 1692 GGTCACC G^GT[ACGT]ACC
EcoT22I 2640 ATGCAT ATGCA^T
FspI 2288 TGCGCA TGC^GCA
Hin1I 1625 GGCGCC G[GA]^CG[CT]C
HindIII 1683 AAGCTT A^AGCTT
KasI 1625 GGCGCC G^GCGCC
KpnI 1367 GGTACC GGTAC^C
KspI 2873 CCGCGG CCGC^GG
MamI 2265 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2177 TCCGGA T^CCGGA
NarI 1625 GGCGCC GG^CGCC
NcoI 1889 CCATGG C^CATGG
NsiI 2640 ATGCAT ATGCA^T
PflFI 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2873 CCGCGG CCGC^GG
SfoI 1625 GGCGCC GGC^GCC
SphI 2642 GCATGC GCATG^C
SspBI 1343 TGTACA T^GTACA
Tth111I 2509 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2460 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 451 AAATTC [GA]^AATT[CT]
1771 GAATTT
AocI 52 CCTGAGG CC^T[ACGT]AGG
1103 CCTTAGG
ApaI 2078 GGGCCC GGGCC^C
2590 GGGCCC
ApoI 451 AAATTC [GA]^AATT[CT]
1771 GAATTT
AvaI 427 CTCGGG C^[CT]CG[GA]G
995 CCCGAG
BbrPI 292 CACGTG CAC^GTG
1721 CACGTG
BglII 733 AGATCT A^GATCT
2348 AGATCT
BsaAI 292 CACGTG [CT]AC^GT[GA]
1721 CACGTG
BsmAI 793 GTCTC GTCTC(1/5)
838 GTCTC
BsoBI 427 CTCGGG C^[CT]CG[GA]G
995 CCCGAG
BstAPI 381 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2642 GCATGCTCTGC
Bsu36I 52 CCTGAGG CC^T[ACGT]AGG
1103 CCTTAGG
Eco81I 52 CCTGAGG CC^T[ACGT]AGG
1103 CCTTAGG
HgaI 484 GACGC GACGC(5/10)
562 GCGTC
PflMI 134 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
748 CCACTCTCTGG
PmaCI 292 CACGTG CAC^GTG
1721 CACGTG
PmlI 292 CACGTG CAC^GTG
1721 CACGTG
SmlI 773 CTCAAG C^T[CT][GA]AG
1812 CTCAAG
TaqI 557 TCGA T^CGA
1517 TCGA
TthHB8I 557 TCGA T^CGA
1517 TCGA
Van91I 134 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
748 CCACTCTCTGG
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII