Restriction map for
pF1KE2674
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
Bsp1407I BspEI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HindIII HpaI KasI KspI MluI
MroI NarI NcoI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 2059 GGTACC G^GTACC
AccII 694 CGCG CG^CG
AcyI 149 GGCGTC G[GA]^CG[CT]C
AflIII 1825 ACATGT A^C[GA][CT]GT
Alw44I 2148 GTGCAC G^TGCAC
ApaLI 2148 GTGCAC G^TGCAC
AseI 609 ATTAAT AT^TAAT
AsnI 609 ATTAAT AT^TAAT
Asp700I 1952 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2059 GGTACC G^GTACC
BanI 2059 GGTACC G^G[CT][GA]CC
BanII 1462 GAGCCC G[GA]GC[CT]^C
BbsI 2803 GAAGAC GAAGAC(2/6)
BcnI 842 CCGGG CC^[GC]GG
BglII 1995 AGATCT A^GATCT
BpuAI 2803 GAAGAC GAAGAC(2/6)
BsaHI 149 GGCGTC G[GA]^CG[CT]C
BsaI 2840 GAGACC GGTCTC(1/5)
BsmBI 2655 CGTCTC CGTCTC(1/5)
BspDI 2276 ATCGAT AT^CGAT
BspLU11I 1825 ACATGT A^CATGT
BsrBI 1314 GAGCGG CCGCTC(-3/-3)
BsrDI 861 CATTGC GCAATG(2/0)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BssSI 86 CACGAG CACGAG(-5/-1)
BstUI 694 CGCG CG^CG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
ClaI 2276 ATCGAT AT^CGAT
DraII 1479 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1479 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 2844 CCTTGG C^C[AT][AT]GG
HgaI 150 GCGTC GACGC(5/10)
Hin1I 149 GGCGTC G[GA]^CG[CT]C
HincII 730 GTTGAC GT[CT]^[GA]AC
HindII 730 GTTGAC GT[CT]^[GA]AC
KpnI 2059 GGTACC GGTAC^C
MaeII 1025 ACGT A^CGT
MfeI 299 CAATTG C^AATTG
MunI 299 CAATTG C^AATTG
MvnI 694 CGCG CG^CG
NaeI 13 GCCGGC GCC^GGC
NciI 842 CCGGG CC^[GC]GG
NgoMIV 13 GCCGGC G^CCGGC
PaeR7I 221 CTCGAG C^TCGAG
PpuMI 1479 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 609 ATTAAT AT^TAAT
PvuI 2278 CGATCG CGAT^CG
SapI 2863 GAAGAGC GCTCTTC(1/4)
SspI 2232 AATATT AAT^ATT
StuI 1009 AGGCCT AGG^CCT
StyI 2844 CCTTGG C^C[AT][AT]GG
XhoI 221 CTCGAG C^TCGAG
XmnI 1952 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 1829 GTGCTC G[AT]GC[AT]^C
2148 GTGCAC
AvaI 221 CTCGAG C^[CT]CG[GA]G
2217 CTCGGG
BalI 1943 TGGCCA TGG^CCA
2347 TGGCCA
BciVI 2631 GGATAC GTATCC(6/5)
2451 GTATCC
BclI 405 TGATCA T^GATCA
1647 TGATCA
BfaI 1999 CTAG C^TAG
2323 CTAG
BsgI 1375 GTGCAG GTGCAG(16/14)
1913 GTGCAG
BsiHKAI 1829 GTGCTC G[AT]GC[AT]^C
2148 GTGCAC
BsoBI 221 CTCGAG C^[CT]CG[GA]G
2217 CTCGGG
BspMI 1842 GCAGGT ACCTGC(4/8)
1964 GCAGGT
BstXI 2759 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2903 CCACCCTTCTGG
CfoI 693 GCGC GCG^C
2043 GCGC
EcoNI 1471 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2907 CCTTCTGGAGG
EcoRV 2371 GATATC GAT^ATC
2547 GATATC
FbaI 405 TGATCA T^GATCA
1647 TGATCA
HapII 14 CCGG C^CGG
842 CCGG
HhaI 693 GCGC GCG^C
2043 GCGC
HinP1I 693 GCGC G^CGC
2043 GCGC
HpaII 14 CCGG C^CGG
842 CCGG
MaeI 1999 CTAG C^TAG
2323 CTAG
MluNI 1943 TGGCCA TGG^CCA
2347 TGGCCA
MscI 1943 TGGCCA TGG^CCA
2347 TGGCCA
MspI 14 CCGG C^CGG
842 CCGG
NdeI 791 CATATG CA^TATG
1165 CATATG
NspI 1279 ACATGC [GA]CATG^[CT]
1825 ACATGT
PstI 632 CTGCAG CTGCA^G
1060 CTGCAG
PvuII 530 CAGCTG CAG^CTG
2726 CAGCTG
ScaI 46 AGTACT AGT^ACT
876 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BmyI BpmI
BsaBI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII