Restriction map for
pF1KE2648
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspEI AviII
AvrII BalI BbeI BbrPI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiWI BspDI BspEI BsrBI BssHII
BssSI BstAPI BstBI CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MunI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 792 GGTACC G^GTACC
AflIII 315 ACATGT A^C[GA][CT]GT
AocI 859 CCTAAGG CC^T[ACGT]AGG
Asp718I 792 GGTACC G^GTACC
AspI 541 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 33 CCCGGG C^[CT]CG[GA]G
BamHI 2023 GGATCC G^GATCC
BanI 792 GGTACC G^G[CT][GA]CC
BbsI 226 GAAGAC GAAGAC(2/6)
BbvCI 2063 CCTCAGC CCTCAGC(-5/-2)
BglII 1292 AGATCT A^GATCT
BpuAI 226 GAAGAC GAAGAC(2/6)
BsaAI 1107 TACGTG [CT]AC^GT[GA]
BsaBI 1287 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1060 GAGACC GGTCTC(1/5)
BseRI 1129 GAGGAG GAGGAG(10/8)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmBI 1148 GAGACG CGTCTC(1/5)
BsmI 720 GCATTC GAATGC(1/-1)
BsoBI 33 CCCGGG C^[CT]CG[GA]G
BspHI 1421 TCATGA T^CATGA
BspLU11I 315 ACATGT A^CATGT
BsrDI 641 CATTGC GCAATG(2/0)
BsrFI 60 GCCGGC [GA]^CCGG[CT]
Bst1107I 468 GTATAC GTA^TAC
BstEII 743 GGTCACC G^GT[ACGT]ACC
BstPI 743 GGTCACC G^GT[ACGT]ACC
BstXI 1001 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 468 GTATAC GTA^TAC
Bsu36I 859 CCTAAGG CC^T[ACGT]AGG
CfoI 55 GCGC GCG^C
Cfr10I 60 GCCGGC [GA]^CCGG[CT]
DraII 1502 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 859 CCTAAGG CC^T[ACGT]AGG
EcoNI 859 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1502 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 743 GGTCACC G^GT[ACGT]ACC
EcoT22I 1404 ATGCAT ATGCA^T
HhaI 55 GCGC GCG^C
HinP1I 55 GCGC G^CGC
HindIII 2008 AAGCTT A^AGCTT
KpnI 792 GGTACC GGTAC^C
MamI 1287 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 60 GCCGGC GCC^GGC
NcoI 800 CCATGG C^CATGG
NgoMIV 60 GCCGGC G^CCGGC
NsiI 1404 ATGCAT ATGCA^T
NspI 315 ACATGT [GA]CATG^[CT]
PflFI 541 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 69 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1502 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1421 TCATGA T^CATGA
SacI 931 GAGCTC GAGCT^C
SmaI 33 CCCGGG CCC^GGG
SspI 1544 AATATT AAT^ATT
TaqI 1860 TCGA T^CGA
Tsp45I 744 GTCAC ^GT[GC]AC
Tth111I 541 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1860 TCGA T^CGA
Van91I 69 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1144 TCTAGA T^CTAGA
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 468 GTATAC GT^[AC][GT]AC
2269 GTCTAC
AccII 9 CGCG CG^CG
558 CGCG
AseI 2169 ATTAAT AT^TAAT
2223 ATTAAT
AsnI 2169 ATTAAT AT^TAAT
2223 ATTAAT
AspHI 931 GAGCTC G[AT]GC[AT]^C
1335 GAGCAC
BanII 931 GAGCTC G[GA]GC[CT]^C
1132 GAGCCC
BcnI 33 CCCGG CC^[GC]GG
34 CCGGG
BfaI 503 CTAG C^TAG
1145 CTAG
BsiHKAI 931 GAGCTC G[AT]GC[AT]^C
1335 GAGCAC
BsmAI 1060 GAGAC GTCTC(1/5)
1148 GAGAC
Bsp1407I 1367 TGTACA T^GTACA
1435 TGTACA
BspMI 865 GCAGGT ACCTGC(4/8)
2265 GCAGGT
BsrGI 1367 TGTACA T^GTACA
1435 TGTACA
BstUI 9 CGCG CG^CG
558 CGCG
BstYI 1292 AGATCT [GA]^GATC[CT]
2023 GGATCC
DsaI 800 CCATGG C^C[GA][CT]GG
2027 CCGTGG
HapII 34 CCGG C^CGG
61 CCGG
HpaII 34 CCGG C^CGG
61 CCGG
MaeI 503 CTAG C^TAG
1145 CTAG
MflI 1292 AGATCT [GA]^GATC[CT]
2023 GGATCC
MspI 34 CCGG C^CGG
61 CCGG
MvnI 9 CGCG CG^CG
558 CGCG
NciI 33 CCCGG CC^[GC]GG
34 CCGGG
NdeI 437 CATATG CA^TATG
479 CATATG
PshBI 2169 ATTAAT AT^TAAT
2223 ATTAAT
SapI 2301 GAAGAGC GCTCTTC(1/4)
1696 GCTCTTC
SfcI 1067 CTACAG C^T[GA][CT]AG
2319 CTACAG
SspBI 1367 TGTACA T^GTACA
1435 TGTACA
XhoII 1292 AGATCT [GA]^GATC[CT]
2023 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BciVI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI