Restriction map for
pF1KE2742
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DraI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SacI SacII SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AatII 2878 GACGTC GACGT^C
BbvCI 1110 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2101 CACGTA [CT]AC^GT[GA]
BsaI 3943 GGTCTC GGTCTC(1/5)
BsmBI 3891 CGTCTC CGTCTC(1/5)
BsmI 3967 GCATTC GAATGC(1/-1)
BspHI 990 TCATGA T^CATGA
BssSI 3783 CACGAG CACGAG(-5/-1)
CpoI 1922 CGGACCG CG^G[AT]CCG
DraIII 160 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2218 GACAACGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2260 CGGCCG C^GGCCG
EclXI 2260 CGGCCG C^GGCCG
Eco52I 2260 CGGCCG C^GGCCG
EcoRI 2630 GAATTC G^AATTC
HindIII 3925 AAGCTT A^AGCTT
HpaI 1105 GTTAAC GTT^AAC
NdeI 1636 CATATG CA^TATG
RcaI 990 TCATGA T^CATGA
RsrII 1922 CGGACCG CG^G[AT]CCG
SalI 2881 GTCGAC G^TCGAC
ScaI 1327 AGTACT AGT^ACT
SexAI 1394 ACCAGGT A^CC[AT]GGT
SphI 321 GCATGC GCATG^C
SspI 926 AATATT AAT^ATT
StuI 2240 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 24 enzymes) #
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AgeI 614 ACCGGT A^CCGGT
3132 ACCGGT
AvaI 890 CCCGGG C^[CT]CG[GA]G
2324 CCCGGG
BbsI 847 GAAGAC GAAGAC(2/6)
3325 GTCTTC
BcgI 272 CGATTGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2238 CGAGGCCTCTGC
BpuAI 847 GAAGAC GAAGAC(2/6)
3325 GTCTTC
BsaWI 614 ACCGGT [AT]^CCGG[AT]
3132 ACCGGT
BsiEI 1164 CGACCG CG[GA][CT]^CG
2260 CGGCCG
BsmAI 3892 GTCTC GTCTC(1/5)
3944 GTCTC
BsoBI 890 CCCGGG C^[CT]CG[GA]G
2324 CCCGGG
Bsp1407I 1287 TGTACA T^GTACA
3011 TGTACA
BsrGI 1287 TGTACA T^GTACA
3011 TGTACA
EcoNI 27 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3144 CCTGCAGAAGG
HincII 1105 GTTAAC GT[CT]^[GA]AC
2881 GTCGAC
HindII 1105 GTTAAC GT[CT]^[GA]AC
2881 GTCGAC
NaeI 2331 GCCGGC GCC^GGC
2699 GCCGGC
NgoMIV 2331 GCCGGC G^CCGGC
2699 GCCGGC
NspI 321 GCATGC [GA]CATG^[CT]
1457 ACATGC
PinAI 614 ACCGGT A^CCGGT
3132 ACCGGT
SmaI 890 CCCGGG CCC^GGG
2324 CCCGGG
SmlI 451 CTTGAG C^T[CT][GA]AG
3946 CTCAAG
SspBI 1287 TGTACA T^GTACA
3011 TGTACA
TfiI 1668 GATTC G^A[AT]TC
3501 GATTC
XmaCI 890 CCCGGG C^CCGGG
2324 CCCGGG
XmaI 890 CCCGGG C^CCGGG
2324 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaII BalI
BamHI BanI BanII BbvI BclI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI