Restriction map for
pF1KE2602
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BanII
BbeI BcgI BciVI BfrI BglI
BglII BlnI BsaHI BsiEI BsiWI
BsmBI BsmI BsoBI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1039 GTAGAC GT^[AC][GT]AC
AccIII 999 TCCGGA T^CCGGA
AcsI 1937 AAATTC [GA]^AATT[CT]
ApoI 1937 AAATTC [GA]^AATT[CT]
AspHI 675 GAGCAC G[AT]GC[AT]^C
BamHI 269 GGATCC G^GATCC
BbrPI 103 CACGTG CAC^GTG
BcnI 266 CCCGG CC^[GC]GG
BlpI 48 GCTGAGC GC^T[ACGT]AGC
BmyI 675 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaAI 103 CACGTG [CT]AC^GT[GA]
BsaBI 1859 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 999 TCCGGA [AT]^CCGG[AT]
BseAI 999 TCCGGA T^CCGGA
BseRI 543 CTCCTC GAGGAG(10/8)
BsgI 290 GTGCAG GTGCAG(16/14)
BsiHKAI 675 GAGCAC G[AT]GC[AT]^C
Bsp1286I 675 GAGCAC G[AGT]GC[ACT]^C
BspDI 64 ATCGAT AT^CGAT
BspEI 999 TCCGGA T^CCGGA
BspHI 1116 TCATGA T^CATGA
BssSI 40 CTCGTG CACGAG(-5/-1)
BstXI 1827 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 48 GCTGAGC GC^T[ACGT]AGC
CfoI 58 GCGC GCG^C
ClaI 64 ATCGAT AT^CGAT
EcoNI 153 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1232 GATATC GAT^ATC
FauI 458 CCCGC CCCGC(4/6)
HhaI 58 GCGC GCG^C
HinP1I 58 GCGC G^CGC
HpaI 442 GTTAAC GTT^AAC
MamI 1859 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 999 TCCGGA T^CCGGA
MseI 443 TTAA T^TAA
MwoI 1282 GCAGCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 266 CCCGG CC^[GC]GG
NcoI 1379 CCATGG C^CATGG
NspI 252 GCATGT [GA]CATG^[CT]
PmaCI 103 CACGTG CAC^GTG
PmlI 103 CACGTG CAC^GTG
PshAI 1420 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1116 TCATGA T^CATGA
SbfI 227 CCTGCAGG CCTGCA^GG
SpeI 679 ACTAGT A^CTAGT
Sse8387I 227 CCTGCAGG CCTGCA^GG
Tru9I 443 TTAA T^TAA
XcmI 1845 CCATACCACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1740 GACTGGGTC
BanI 454 GGCACC G^G[CT][GA]CC
1051 GGCACC
BbvCI 1286 CCTCAGC CCTCAGC(-5/-2)
1730 CCTCAGC
BclI 1952 TGATCA T^GATCA
2048 TGATCA
BsrDI 2001 CATTGC GCAATG(2/0)
2060 GCAATG
BstYI 269 GGATCC [GA]^GATC[CT]
1858 GGATCT
DsaI 934 CCACGG C^C[GA][CT]GG
1379 CCATGG
EarI 1126 GAAGAG CTCTTC(1/4)
1631 GAAGAG
EcoT14I 1379 CCATGG C^C[AT][AT]GG
1976 CCTTGG
FbaI 1952 TGATCA T^GATCA
2048 TGATCA
HapII 267 CCGG C^CGG
1000 CCGG
HincII 442 GTTAAC GT[CT]^[GA]AC
2008 GTCAAC
HindII 442 GTTAAC GT[CT]^[GA]AC
2008 GTCAAC
HpaII 267 CCGG C^CGG
1000 CCGG
Ksp632I 1126 GAAGAG CTCTTC(1/4)
1631 GAAGAG
MflI 269 GGATCC [GA]^GATC[CT]
1858 GGATCT
MspI 267 CCGG C^CGG
1000 CCGG
PflFI 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1740 GACTGGGTC
PvuII 1277 CAGCTG CAG^CTG
2177 CAGCTG
StyI 1379 CCATGG C^C[AT][AT]GG
1976 CCTTGG
Tth111I 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1740 GACTGGGTC
XhoII 269 GGATCC [GA]^GATC[CT]
1858 GGATCT
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbsI BbvI BfaI BpmI
BpuAI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MvaI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I