Restriction map for
pF1KE2695
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# NO CUT ENZYMES ( 44 enzymes) #
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AatII AclI AcsI AflII Alw44I
ApaLI ApoI AscI AseI AsnI
BbrPI BciVI BfrI BsiWI Bst1107I
BstBI BstZ17I DraI EcoRI EcoRV
EcoT22I FseI HpaI MfeI MluI
MunI NdeI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I SbfI SfiI SfuI SnaBI
SpeI Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AgeI 1307 ACCGGT A^CCGGT
AhdI 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3518 TGCGCA TGC^GCA
AvrII 5160 CCTAGG C^CTAGG
BglII 1317 AGATCT A^GATCT
BlnI 5160 CCTAGG C^CTAGG
BsmBI 5064 CGTCTC CGTCTC(1/5)
BspDI 141 ATCGAT AT^CGAT
BspHI 647 TCATGA T^CATGA
BstEII 6937 GGTTACC G^GT[ACGT]ACC
BstPI 6937 GGTTACC G^GT[ACGT]ACC
ClaI 141 ATCGAT AT^CGAT
CpoI 6116 CGGACCG CG^G[AT]CCG
Eam1105I 3105 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 6937 GGTTACC G^GT[ACGT]ACC
FspI 3518 TGCGCA TGC^GCA
HindIII 2194 AAGCTT A^AGCTT
MseI 3397 TTAA T^TAA
NheI 6535 GCTAGC G^CTAGC
PaeR7I 5303 CTCGAG C^TCGAG
PinAI 1307 ACCGGT A^CCGGT
PshAI 4770 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 143 CGATCG CGAT^CG
RcaI 647 TCATGA T^CATGA
RsrII 6116 CGGACCG CG^G[AT]CCG
SalI 2533 GTCGAC G^TCGAC
SphI 4850 GCATGC GCATG^C
SspI 4867 AATATT AAT^ATT
Tru9I 3397 TTAA T^TAA
XhoI 5303 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 2506 CCTGAGG CC^T[ACGT]AGG
6820 CCTGAGG
Aor51HI 6056 AGCGCT AGC^GCT
6374 AGCGCT
AspI 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4615 GACTTTGTC
BbeI 2845 GGCGCC GGCGC^C
3685 GGCGCC
BcgI 5208 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4316 GCATTGACTTCG
BlpI 4006 GCTCAGC GC^T[ACGT]AGC
6681 GCTCAGC
Bpu1102I 4006 GCTCAGC GC^T[ACGT]AGC
6681 GCTCAGC
BsaBI 438 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4242 GATCATCATC
BspLU11I 5243 ACATGT A^CATGT
5264 ACATGT
Bsu36I 2506 CCTGAGG CC^T[ACGT]AGG
6820 CCTGAGG
CelII 4006 GCTCAGC GC^T[ACGT]AGC
6681 GCTCAGC
Eco47III 6056 AGCGCT AGC^GCT
6374 AGCGCT
Eco81I 2506 CCTGAGG CC^T[ACGT]AGG
6820 CCTGAGG
EcoNI 6198 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6816 CCTGCCTGAGG
KasI 2845 GGCGCC G^GCGCC
3685 GGCGCC
MamI 438 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4242 GATCATCATC
NarI 2845 GGCGCC GG^CGCC
3685 GGCGCC
PflFI 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4615 GACTTTGTC
ScaI 854 AGTACT AGT^ACT
1769 AGTACT
SexAI 1046 ACCTGGT A^CC[AT]GGT
1790 ACCTGGT
SfoI 2845 GGCGCC GGC^GCC
3685 GGCGCC
SgrAI 2755 CACCGGCG C[GA]^CCGG[CT]G
2841 CGCCGGCG
Tth111I 241 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4615 GACTTTGTC
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# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
Acc65I AccI AccII AccIII AciI
AcyI AfaI AflIII AluI AlwI
AlwNI ApaI Asp700I Asp718I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NotI NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI