Restriction map for
pF1KE2689
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbeI BciVI BclI
BfaI BfrI BlnI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SapI
SbfI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AhdI 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1084 GTGCAC G^TGCAC
AocI 532 CCTGAGG CC^T[ACGT]AGG
ApaLI 1084 GTGCAC G^TGCAC
AspEI 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 355 CACGTG CAC^GTG
BcgI 1504 CGAGGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 11 AGATCT A^GATCT
BlpI 444 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 444 GCTCAGC GC^T[ACGT]AGC
BsaAI 355 CACGTG [CT]AC^GT[GA]
BsaI 1354 GAGACC GGTCTC(1/5)
BsiEI 712 CGGCCG CG[GA][CT]^CG
BsmI 174 GCATTC GAATGC(1/-1)
BsrDI 746 GCAATG GCAATG(2/0)
BsrFI 590 ACCGGC [GA]^CCGG[CT]
BstBI 823 TTCGAA TT^CGAA
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
CelII 444 GCTCAGC GC^T[ACGT]AGC
CfoI 1050 GCGC GCG^C
Cfr10I 590 ACCGGC [GA]^CCGG[CT]
DraIII 296 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 712 CGGCCG C^GGCCG
Eam1105I 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 712 CGGCCG C^GGCCG
Eco52I 712 CGGCCG C^GGCCG
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
EcoNI 309 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1211 CCCGC CCCGC(4/6)
HgaI 1047 GACGC GACGC(5/10)
HhaI 1050 GCGC GCG^C
HinP1I 1050 GCGC G^CGC
KspI 715 CCGCGG CCGC^GG
NotI 711 GCGGCCGC GC^GGCCGC
NspV 823 TTCGAA TT^CGAA
PflFI 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 588 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 355 CACGTG CAC^GTG
PmlI 355 CACGTG CAC^GTG
SacI 1456 GAGCTC GAGCT^C
SacII 715 CCGCGG CCGC^GG
ScaI 1178 AGTACT AGT^ACT
SexAI 1055 ACCTGGT A^CC[AT]GGT
SfcI 1014 CTACAG C^T[GA][CT]AG
SfuI 823 TTCGAA TT^CGAA
SmaI 505 CCCGGG CCC^GGG
TfiI 112 GAATC G^A[AT]TC
Tth111I 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 588 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 505 CCCGGG C^CCGGG
XmaI 505 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 816 GACGTC GACGT^C
1570 GACGTC
AcyI 816 GACGTC G[GA]^CG[CT]C
1570 GACGTC
AflIII 354 ACACGT A^C[GA][CT]GT
686 ACACGT
AlwNI 265 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1139 CAGATCCTG
ApaI 55 GGGCCC GGGCC^C
528 GGGCCC
AspHI 1084 GTGCAC G[AT]GC[AT]^C
1456 GAGCTC
BalI 377 TGGCCA TGG^CCA
1271 TGGCCA
BbsI 723 GAAGAC GAAGAC(2/6)
661 GTCTTC
BglI 394 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1598 GCCTTCGGGGC
BpuAI 723 GAAGAC GAAGAC(2/6)
661 GTCTTC
BsaHI 816 GACGTC G[GA]^CG[CT]C
1570 GACGTC
BseRI 33 CTCCTC GAGGAG(10/8)
121 GAGGAG
BsiHKAI 1084 GTGCAC G[AT]GC[AT]^C
1456 GAGCTC
BsmBI 814 GAGACG CGTCTC(1/5)
1045 GAGACG
BstAPI 295 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1138 GCAGATCCTGC
EarI 118 GAAGAG CTCTTC(1/4)
130 GAAGAG
Hin1I 816 GACGTC G[GA]^CG[CT]C
1570 GACGTC
Ksp632I 118 GAAGAG CTCTTC(1/4)
130 GAAGAG
MluNI 377 TGGCCA TGG^CCA
1271 TGGCCA
MscI 377 TGGCCA TGG^CCA
1271 TGGCCA
MseI 557 TTAA T^TAA
1484 TTAA
NspI 602 ACATGC [GA]CATG^[CT]
1088 ACATGC
PleI 988 GACTC GAGTC(4/5)
1009 GACTC
PpuMI 75 GGGTCCC [GA]G^G[AT]CC[CT]
630 GGGACCC
PvuII 207 CAGCTG CAG^CTG
880 CAGCTG
StuI 536 AGGCCT AGG^CCT
1373 AGGCCT
Tru9I 557 TTAA T^TAA
1484 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII