Restriction map for
pF1KE2735
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NarI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I TspRI
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 682 GACGTC GACGT^C
AccII 441 CGCG CG^CG
AcsI 478 AAATTT [GA]^AATT[CT]
AfaI 615 GTAC GT^AC
AlwNI 25 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 538 GGGCCC GGGCC^C
ApoI 478 AAATTT [GA]^AATT[CT]
Asp700I 264 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 679 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 259 CACGTG CAC^GTG
BcgI 639 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 196 CTAG C^TAG
BsaAI 259 CACGTG [CT]AC^GT[GA]
BsiEI 666 CGGCCG CG[GA][CT]^CG
BsrFI 428 GCCGGC [GA]^CCGG[CT]
BssSI 364 CACGAG CACGAG(-5/-1)
BstF5I 515 GGATG GGATG(2/0)
BstUI 441 CGCG CG^CG
BstXI 315 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 428 GCCGGC [GA]^CCGG[CT]
DpnII 238 GATC ^GATC
DraIII 445 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 621 CCACGG C^C[GA][CT]GG
EagI 666 CGGCCG C^GGCCG
EclXI 666 CGGCCG C^GGCCG
Eco52I 666 CGGCCG C^GGCCG
EcoT14I 5 CCAAGG C^C[AT][AT]GG
FauI 93 GCGGG CCCGC(4/6)
FokI 515 GGATG GGATG(9/13)
HaeII 309 GGCGCT [GA]GCGC^[CT]
HincII 349 GTCAAC GT[CT]^[GA]AC
HindII 349 GTCAAC GT[CT]^[GA]AC
HphI 656 TCACC GGTGA(8/7)
MaeI 196 CTAG C^TAG
MboI 238 GATC ^GATC
MslI 510 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 441 CGCG CG^CG
NaeI 428 GCCGGC GCC^GGC
NdeII 238 GATC ^GATC
NgoMIV 428 GCCGGC G^CCGGC
PflFI 679 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 259 CACGTG CAC^GTG
PmlI 259 CACGTG CAC^GTG
PstI 79 CTGCAG CTGCA^G
RsaI 615 GTAC GT^AC
Sau3AI 238 GATC ^GATC
SexAI 446 ACCTGGT A^CC[AT]GGT
SfaNI 27 GCATC GCATC(5/9)
SfcI 79 CTGCAG C^T[GA][CT]AG
SmlI 153 CTTGAG C^T[CT][GA]AG
StuI 401 AGGCCT AGG^CCT
StyI 5 CCAAGG C^C[AT][AT]GG
Tsp509I 479 AATT ^AATT
Tth111I 679 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 264 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 412 GACGCC G[GA]^CG[CT]C
682 GACGTC
AvaII 225 GGACC G^G[AT]CC
335 GGACC
BanII 538 GGGCCC G[GA]GC[CT]^C
625 GGGCTC
BcnI 345 CCCGG CC^[GC]GG
395 CCGGG
BsaHI 412 GACGCC G[GA]^CG[CT]C
682 GACGTC
BsmBI 106 GAGACG CGTCTC(1/5)
571 GAGACG
BsmFI 559 GGGAC GGGAC(10/14)
201 GTCCC
BspMI 281 ACCTGC ACCTGC(4/8)
337 ACCTGC
BsrDI 170 GCAATG GCAATG(2/0)
320 GCAATG
EaeI 618 CGGCCA [CT]^GGCC[GA]
666 CGGCCG
HgaI 412 GACGC GACGC(5/10)
439 GACGC
Hin1I 412 GACGCC G[GA]^CG[CT]C
682 GACGTC
HinfI 70 GACTC G^A[ACGT]TC
199 GAGTC
MaeIII 47 GTGAC ^GT[ACGT]AC
457 GTCAC
MboII 179 GAAGA GAAGA(8/7)
235 GAAGA
NciI 345 CCCGG CC^[GC]GG
395 CCGGG
PleI 70 GACTC GAGTC(4/5)
199 GAGTC
PpuMI 224 AGGACCT [GA]G^G[AT]CC[CT]
334 AGGACCT
Tsp45I 47 GTGAC ^GT[GC]AC
457 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MnlI MspA1I MspI MvaI
MwoI NlaIV PvuII Sau96I ScrFI
TseI