Restriction map for
pF1KE2770
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspDI
BspHI BsrFI BssHII Bst1107I BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfoI
SgrAI SnaBI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 3073 GTCTAC GT^[AC][GT]AC
AccII 2085 CGCG CG^CG
AflII 2665 CTTAAG C^TTAAG
AflIII 197 ACATGT A^C[GA][CT]GT
BciVI 2336 GGATAC GTATCC(6/5)
BfrI 2665 CTTAAG C^TTAAG
BglII 3090 AGATCT A^GATCT
Bsp1407I 41 TGTACA T^GTACA
BspLU11I 197 ACATGT A^CATGT
BsrBI 2272 CCGCTC CCGCTC(-3/-3)
BsrGI 41 TGTACA T^GTACA
BstAPI 3173 GCATGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 65 TTCGAA TT^CGAA
BstEII 550 GGTGACC G^GT[ACGT]ACC
BstPI 550 GGTGACC G^GT[ACGT]ACC
BstUI 2085 CGCG CG^CG
EcoO65I 550 GGTGACC G^GT[ACGT]ACC
EcoRI 3535 GAATTC G^AATTC
HaeII 3138 AGCGCC [GA]GCGC^[CT]
HincII 19 GTCAAC GT[CT]^[GA]AC
HindII 19 GTCAAC GT[CT]^[GA]AC
HindIII 2298 AAGCTT A^AGCTT
MaeII 1276 ACGT A^CGT
MvnI 2085 CGCG CG^CG
NcoI 908 CCATGG C^CATGG
NspV 65 TTCGAA TT^CGAA
PflMI 2196 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1578 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1882 CTGCAG CTGCA^G
SapI 557 GAAGAGC GCTCTTC(1/4)
SbfI 1881 CCTGCAGG CCTGCA^GG
ScaI 235 AGTACT AGT^ACT
SexAI 3596 ACCTGGT A^CC[AT]GGT
SfuI 65 TTCGAA TT^CGAA
SphI 3173 GCATGC GCATG^C
Sse8387I 1881 CCTGCAGG CCTGCA^GG
SspBI 41 TGTACA T^GTACA
SspI 3156 AATATT AAT^ATT
StuI 1850 AGGCCT AGG^CCT
Van91I 2196 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 1527 TCCGGA T^CCGGA
2818 TCCGGA
Alw44I 3564 GTGCAC G^TGCAC
3601 GTGCAC
AlwI 1702 GATCC GGATC(4/5)
504 GGATC
ApaLI 3564 GTGCAC G^TGCAC
3601 GTGCAC
BalI 2450 TGGCCA TGG^CCA
2697 TGGCCA
BanI 1640 GGCACC G^G[CT][GA]CC
3288 GGCACC
BbsI 1291 GAAGAC GAAGAC(2/6)
1956 GAAGAC
BlpI 1159 GCTGAGC GC^T[ACGT]AGC
3549 GCTCAGC
Bpu1102I 1159 GCTGAGC GC^T[ACGT]AGC
3549 GCTCAGC
BpuAI 1291 GAAGAC GAAGAC(2/6)
1956 GAAGAC
BseAI 1527 TCCGGA T^CCGGA
2818 TCCGGA
BsmI 323 GCATTC GAATGC(1/-1)
1686 GCATTC
BspEI 1527 TCCGGA T^CCGGA
2818 TCCGGA
BssSI 1674 CACGAG CACGAG(-5/-1)
3519 CACGAG
BstYI 1701 AGATCC [GA]^GATC[CT]
3090 AGATCT
CelII 1159 GCTGAGC GC^T[ACGT]AGC
3549 GCTCAGC
EcoT22I 3171 ATGCAT ATGCA^T
3300 ATGCAT
MflI 1701 AGATCC [GA]^GATC[CT]
3090 AGATCT
MluNI 2450 TGGCCA TGG^CCA
2697 TGGCCA
MroI 1527 TCCGGA T^CCGGA
2818 TCCGGA
MscI 2450 TGGCCA TGG^CCA
2697 TGGCCA
NsiI 3171 ATGCAT ATGCA^T
3300 ATGCAT
SacI 2040 GAGCTC GAGCT^C
2427 GAGCTC
SfcI 1882 CTGCAG C^T[GA][CT]AG
3086 CTGTAG
SpeI 346 ACTAGT A^CTAGT
2182 ACTAGT
XhoII 1701 AGATCC [GA]^GATC[CT]
3090 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI
XmnI